##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934704.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	373877
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.833766185135755	31.0	31.0	34.0	30.0	34.0
2	32.02085445213265	33.0	31.0	34.0	30.0	34.0
3	31.916657082409454	33.0	31.0	34.0	30.0	34.0
4	35.705044707216544	37.0	35.0	37.0	35.0	37.0
5	32.13416979380919	37.0	35.0	37.0	10.0	37.0
6	33.94658136231969	37.0	35.0	37.0	19.0	37.0
7	20.642855805518927	32.0	0.0	37.0	0.0	37.0
8	28.02310385501114	35.0	17.0	37.0	17.0	37.0
9	34.73320370068231	35.0	32.0	39.0	31.0	39.0
10	36.06322667615285	37.0	35.0	39.0	32.0	39.0
11	36.492584459595	37.0	35.0	39.0	32.0	39.0
12	36.86201344292374	39.0	35.0	39.0	33.0	39.0
13	36.65286711940023	39.0	35.0	39.0	33.0	39.0
14	37.94094046972668	40.0	37.0	41.0	33.0	41.0
15	38.126774313477426	40.0	37.0	41.0	33.0	41.0
16	38.22433581097527	40.0	37.0	41.0	34.0	41.0
17	38.19844761779944	40.0	37.0	41.0	34.0	41.0
18	38.08936896358963	40.0	37.0	41.0	33.0	41.0
19	38.0424069948138	40.0	37.0	41.0	33.0	41.0
20	38.03707636468678	40.0	37.0	41.0	34.0	41.0
21	37.94131492442702	40.0	37.0	41.0	33.0	41.0
22	37.9421413994442	40.0	37.0	41.0	33.0	41.0
23	37.8899129927757	40.0	36.0	41.0	33.0	41.0
24	37.7662279305761	40.0	36.0	41.0	33.0	41.0
25	37.62772783562509	39.0	36.0	41.0	33.0	41.0
26	37.499239054555375	39.0	36.0	41.0	33.0	41.0
27	37.43497727862372	39.0	36.0	41.0	33.0	41.0
28	37.377396844416744	39.0	36.0	41.0	33.0	41.0
29	37.33052581463957	39.0	36.0	41.0	32.0	41.0
30	37.13891199512139	39.0	35.0	41.0	32.0	41.0
31	37.003709776209824	39.0	35.0	41.0	31.0	41.0
32	36.89783270968795	39.0	35.0	41.0	31.0	41.0
33	36.724794517983185	39.0	35.0	40.0	31.0	41.0
34	36.59024224544436	39.0	35.0	40.0	30.0	41.0
35	36.38945696044421	39.0	35.0	40.0	30.0	41.0
36	36.19961377672336	39.0	35.0	40.0	30.0	41.0
37	36.106227449134344	39.0	35.0	40.0	29.0	41.0
38	35.95883940440305	39.0	35.0	40.0	29.0	41.0
39	35.88085118902741	39.0	35.0	40.0	28.0	41.0
40	35.73209638463987	39.0	35.0	40.0	27.0	41.0
41	35.623453702688316	38.0	35.0	40.0	27.0	41.0
42	35.477333454585334	38.0	34.0	40.0	26.0	41.0
43	35.328460964434825	38.0	34.0	40.0	26.0	41.0
44	35.14727035896832	38.0	34.0	40.0	25.0	41.0
45	34.9917566472396	38.0	34.0	40.0	24.0	41.0
46	34.795015473003154	38.0	34.0	40.0	23.0	41.0
47	34.70869296586845	38.0	34.0	40.0	23.0	41.0
48	34.59738898086804	38.0	33.0	40.0	23.0	41.0
49	34.471486611907125	38.0	33.0	40.0	23.0	41.0
50	34.2555439355724	37.0	33.0	40.0	22.0	41.0
51	32.18172286607628	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	22.0
10	17.0
11	11.0
12	10.0
13	9.0
14	13.0
15	15.0
16	29.0
17	64.0
18	116.0
19	262.0
20	442.0
21	791.0
22	1225.0
23	1921.0
24	2746.0
25	3748.0
26	4697.0
27	5552.0
28	6245.0
29	7292.0
30	8823.0
31	11325.0
32	14438.0
33	20418.0
34	27548.0
35	34820.0
36	44482.0
37	69209.0
38	66298.0
39	41274.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.892922003760596	18.609596204099212	23.70779694926406	15.789684842876134
2	30.141998571722784	25.20080133305873	26.288324769910957	18.36887532530752
3	31.02089724695555	23.542769413470204	28.77256423904118	16.663769100533063
4	28.08704466977107	25.2815765612755	27.494603840300417	19.136774928653004
5	22.23565504163134	36.669813869267166	24.100974384623818	16.993556704477676
6	25.91788208421486	35.331138315542276	24.904714652144957	13.846264948097902
7	49.222337827681294	43.02377519879533	5.201978190688381	2.5519087828349964
8	84.73508667288975	4.040098749053833	7.688624868606521	3.5361897094498995
9	78.9385279115859	6.472449495422292	9.843344201435231	4.745678391556582
10	40.94555161189375	27.65454949087534	17.434075912666465	13.965822984564442
11	30.05186197599745	25.45141851464519	26.166894459942707	18.329825049414648
12	26.706376696079193	22.889345961372324	30.30247915758391	20.101798184964572
13	23.4280257945795	24.897225558138103	32.29591550162219	19.378833145660206
14	20.148337554864302	26.791163938942486	32.13489998047486	20.92559852571835
15	19.310628896669225	25.195451980196697	34.70553149832699	20.78838762480709
16	22.865273873493155	24.731128151771838	32.058136766904624	20.345461207830382
17	22.7577518809662	25.01758599753395	29.46958491696467	22.755077204535183
18	23.6128459359629	23.613380871249102	31.689031419424037	21.08474177336397
19	23.121775343227853	26.48197134351672	29.0028538797519	21.393399433503532
20	24.712405416754706	26.05322071162441	29.52602058965916	19.708353281961717
21	23.768512104248188	26.957796280594952	30.00746234724254	19.266229267914316
22	23.662594917579845	23.750591772160362	29.91010412515346	22.67670918510633
23	21.846757088561212	25.984214059704126	29.567745541983058	22.6012833097516
24	21.44716042976701	24.842662158945323	30.933435327661236	22.776742083626434
25	21.358361172257187	26.525836036985424	29.40111320033059	22.714689590426797
26	21.46588316478414	27.24773120571739	29.082291769753155	22.204093859745317
27	21.021351941948822	26.21263142691313	30.99682515907638	21.769191472061667
28	19.313303573100242	26.5651537805214	31.41621442346012	22.705328222918233
29	21.27036431767667	25.905311104989075	30.53036158950671	22.29396298782755
30	22.167450792640363	25.301101699221935	31.454462296423692	21.076985211714014
31	23.398336886195192	25.628482094378636	28.663437440655613	22.309743578770558
32	23.800875689063517	25.655228858688822	28.536390310182224	22.00750514206544
33	22.10753804058554	25.367433674711204	29.35778344214809	23.16724484255517
34	21.435124385827425	24.94536973389644	30.867905755101276	22.751600125174857
35	22.044415676813497	25.175659374607157	29.856075661246877	22.923849287332466
36	22.954608066289182	26.024334206169407	29.009808038472546	22.011249689068865
37	21.846222153275008	25.765692995289896	29.583526132926067	22.80455871850903
38	21.345522725388296	26.624264129646917	29.23421339103502	22.79599975392977
39	21.814660971388985	24.992711506725474	28.88490064914397	24.30772687274157
40	23.39084779218834	24.063528914589558	28.97022282729347	23.575400465928634
41	20.710554540664443	23.91561930795422	27.95946260401148	27.41436354736986
42	21.9877125364759	24.220264953447256	29.421440741206332	24.37058176887051
43	21.85772326192839	24.423807829847785	29.685966240234084	24.03250266798974
44	22.158356892774897	24.73861724577869	29.42545275585286	23.67757310559355
45	21.780692580715048	24.955266036691214	28.437694749877636	24.826346632716106
46	22.063940814759935	25.220059003362067	28.49386295492903	24.22213722694897
47	20.39681499530594	25.722630704750493	30.415350502972906	23.465203796970663
48	21.533819946132017	24.980407995142787	29.777975109461135	23.70779694926406
49	22.115294602235494	23.67784057323665	30.425781741053875	23.781083083473977
50	20.75843124877968	24.710265675609893	30.124880642564268	24.406422433046163
51	20.815401856760378	24.195925397924984	28.13652618374492	26.85214656156971
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	176.0
1	502.0
2	828.0
3	2129.5
4	3431.0
5	2320.5
6	1210.0
7	1117.5
8	1025.0
9	1046.0
10	1067.0
11	1100.0
12	1133.0
13	1148.5
14	1164.0
15	1106.0
16	1048.0
17	1087.0
18	1126.0
19	1221.5
20	1317.0
21	1417.0
22	1517.0
23	1736.5
24	1956.0
25	2474.5
26	3585.5
27	4178.0
28	4989.0
29	5800.0
30	6580.5
31	7361.0
32	8174.0
33	8987.0
34	9860.0
35	10733.0
36	12044.0
37	13355.0
38	14821.0
39	16287.0
40	17638.0
41	18989.0
42	20434.5
43	21880.0
44	22857.5
45	23835.0
46	25678.5
47	27522.0
48	29541.0
49	31560.0
50	31773.0
51	31986.0
52	30772.5
53	29559.0
54	26693.0
55	23827.0
56	21272.0
57	18717.0
58	17071.0
59	15425.0
60	13919.0
61	12413.0
62	11290.0
63	10167.0
64	8805.0
65	7443.0
66	6342.0
67	5241.0
68	4315.5
69	3390.0
70	2752.0
71	2114.0
72	1680.5
73	1247.0
74	1062.0
75	699.0
76	521.0
77	381.0
78	241.0
79	178.5
80	116.0
81	84.0
82	52.0
83	44.5
84	37.0
85	23.5
86	10.0
87	8.0
88	6.0
89	5.0
90	4.0
91	4.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	373877.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.76291305106578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.32676492099453	35.75626662158397
2	8.840476506263853	8.621747744098682
3	4.47259307524197	6.542900017224692
4	2.9810127225039293	5.814514567663199
5	2.278019600140649	5.554143584514105
6	1.853132263621157	5.421847646584991
7	1.4555372018570891	4.968336381172418
8	1.1689832556259212	4.560242308179087
9	0.8682709922738902	3.810548061091288
>10	2.733978041233454	17.52127582471274
>50	0.014526761219278329	0.5010335654709209
>100	0.0055872158535685695	0.4135774618732224
>500	5.587215853568569E-4	0.195345876260277
>1k	5.587215853568569E-4	0.3182203395704373
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1168	0.3124022071429909	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	717	0.19177430010404492	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6746764310187575E-4	0.0	0.0	0.06365729905824642	0.0
2	2.6746764310187575E-4	0.0	0.0	0.3009010984896102	0.0
3	2.6746764310187575E-4	0.0	0.0	0.38863048542702544	0.0
4	2.6746764310187575E-4	0.0	0.0	0.5052463778194433	0.0
5	2.6746764310187575E-4	0.0	0.0	0.8692698400810962	0.0
6	2.6746764310187575E-4	0.0	0.0	1.1961152999515883	0.0
7	2.6746764310187575E-4	0.0	0.0	1.494074254367078	0.0
8	2.6746764310187575E-4	0.0	0.0	2.2721376281504346	0.0
9	2.6746764310187575E-4	0.0	0.0	2.6016577644519456	0.0
10	2.6746764310187575E-4	0.0	0.0	3.472264942748551	0.0
11	2.6746764310187575E-4	0.0	0.0	4.58439540276615	0.0
12	2.6746764310187575E-4	0.0	0.0	5.463561545642016	0.0
13	2.6746764310187575E-4	0.0	0.0	5.82063084918302	0.0
14	2.6746764310187575E-4	0.0	0.0	5.972285002821784	0.0
15	2.6746764310187575E-4	0.0	0.0	6.2483116105029195	0.0
16	2.6746764310187575E-4	0.0	0.0	6.8188200932392204	0.0
17	2.6746764310187575E-4	0.0	0.0	7.460207501397519	0.0
18	2.6746764310187575E-4	0.0	0.0	8.176485849624342	0.0
19	2.6746764310187575E-4	0.0	0.0	8.626366425321697	0.0
20	2.6746764310187575E-4	0.0	0.0	9.05030263963817	0.0
21	2.6746764310187575E-4	0.0	0.0	9.574271752474745	0.0
22	2.6746764310187575E-4	0.0	0.0	10.1239177590491	0.0
23	2.6746764310187575E-4	0.0	0.0	10.697903321145724	0.0
24	2.6746764310187575E-4	0.0	0.0	11.143236946910347	0.0
25	2.6746764310187575E-4	0.0	0.0	11.54283360570455	0.0
26	2.6746764310187575E-4	0.0	0.0	11.911136550255833	0.0
27	2.6746764310187575E-4	0.0	0.0	12.311000676693137	0.0
28	2.6746764310187575E-4	0.0	0.0	12.65228938929113	0.0
29	2.6746764310187575E-4	0.0	0.0	13.032360910138896	0.0
30	2.6746764310187575E-4	0.0	0.0	13.514605070651578	0.0
31	2.6746764310187575E-4	0.0	0.0	13.916876405876799	0.0
32	2.6746764310187575E-4	0.0	0.0	14.318345338172714	0.0
33	2.6746764310187575E-4	0.0	0.0	14.729175637977196	0.0
34	2.6746764310187575E-4	0.0	0.0	15.12609762034038	0.0
35	2.6746764310187575E-4	0.0	0.0	15.564744555027456	0.0
36	2.6746764310187575E-4	0.0	0.0	15.95177023459587	0.0
37	2.6746764310187575E-4	0.0	0.0	16.344947669955626	0.0
38	2.6746764310187575E-4	0.0	0.0	16.756580372689413	0.0
39	2.6746764310187575E-4	0.0	0.0	17.262629153438162	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGT	20	7.027173E-4	45.000004	21
CGAAAAG	20	7.027173E-4	45.000004	2
ACCTATC	20	7.027173E-4	45.000004	3
TATACGG	20	7.027173E-4	45.000004	2
CGTTTAG	20	7.027173E-4	45.000004	2
AACACGG	20	7.027173E-4	45.000004	2
CACGGGT	25	3.8857394E-5	45.0	4
ATAACGG	25	3.8857394E-5	45.0	2
TAAGGCG	30	2.1615579E-6	44.999996	5
CGTTATT	75	0.0	42.0	1
CGTTTTA	230	0.0	41.086956	1
AAGGCGA	110	0.0	40.909092	6
ACAACGA	50	1.0768417E-9	40.5	13
CACAACG	50	1.0768417E-9	40.5	12
CGTTTTT	460	0.0	40.108692	1
GTAGCAA	45	1.922308E-8	40.0	1
AGGCGAT	245	0.0	39.489796	7
AGGTACC	40	3.449877E-7	39.375004	7
TTAATCG	35	6.2369872E-6	38.571426	20
CATACGA	70	0.0	38.571426	18
>>END_MODULE