##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934703.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	366578
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.872196367485227	31.0	31.0	34.0	30.0	34.0
2	32.06366175820698	33.0	31.0	34.0	30.0	34.0
3	31.929106493024676	33.0	31.0	34.0	30.0	34.0
4	35.725133532290535	37.0	35.0	37.0	35.0	37.0
5	31.94613697494121	37.0	35.0	37.0	0.0	37.0
6	33.867433397530675	37.0	35.0	37.0	19.0	37.0
7	20.806098565653148	32.0	0.0	37.0	0.0	37.0
8	28.135469122533266	35.0	17.0	37.0	17.0	37.0
9	34.82879496314563	37.0	32.0	39.0	32.0	39.0
10	36.10849259911942	37.0	35.0	39.0	32.0	39.0
11	36.530086366339496	38.0	35.0	39.0	32.0	39.0
12	36.941985061842225	39.0	37.0	39.0	33.0	39.0
13	36.7548980026079	39.0	37.0	39.0	33.0	39.0
14	38.11387480972672	40.0	37.0	41.0	33.0	41.0
15	38.2563110715864	40.0	37.0	41.0	34.0	41.0
16	38.33025713490717	40.0	37.0	41.0	34.0	41.0
17	38.32340729667356	40.0	37.0	41.0	34.0	41.0
18	38.21193852331564	40.0	37.0	41.0	34.0	41.0
19	38.154711957618844	40.0	37.0	41.0	34.0	41.0
20	38.14813218469193	40.0	37.0	41.0	34.0	41.0
21	38.04791340451418	40.0	37.0	41.0	34.0	41.0
22	38.05428585457938	40.0	37.0	41.0	34.0	41.0
23	38.015011811947254	40.0	37.0	41.0	34.0	41.0
24	37.88385555052404	40.0	36.0	41.0	33.0	41.0
25	37.7588371369804	40.0	36.0	41.0	33.0	41.0
26	37.610473078035234	39.0	36.0	41.0	33.0	41.0
27	37.581319664573435	39.0	36.0	41.0	33.0	41.0
28	37.533007981930176	40.0	36.0	41.0	33.0	41.0
29	37.5403924949124	40.0	36.0	41.0	33.0	41.0
30	37.404110448526644	39.0	36.0	41.0	33.0	41.0
31	37.300836384071054	39.0	36.0	41.0	32.0	41.0
32	37.20976981706485	39.0	35.0	41.0	32.0	41.0
33	37.08301098265581	39.0	35.0	41.0	31.0	41.0
34	36.88397557954924	39.0	35.0	41.0	31.0	41.0
35	36.780106825832426	39.0	35.0	41.0	31.0	41.0
36	36.66134901712596	39.0	35.0	41.0	30.0	41.0
37	36.61029849036221	39.0	35.0	41.0	30.0	41.0
38	36.50723174876834	39.0	35.0	41.0	30.0	41.0
39	36.44404192286498	39.0	35.0	41.0	30.0	41.0
40	36.38011828314847	39.0	35.0	40.0	30.0	41.0
41	36.275229282717454	39.0	35.0	40.0	30.0	41.0
42	36.17475680482735	39.0	35.0	40.0	30.0	41.0
43	36.10093895432895	39.0	35.0	40.0	29.0	41.0
44	35.985367370655084	39.0	35.0	40.0	29.0	41.0
45	35.88208239446993	39.0	35.0	40.0	29.0	41.0
46	35.73666723043936	38.0	35.0	40.0	28.0	41.0
47	35.726860313493994	38.0	35.0	40.0	28.0	41.0
48	35.61119598011883	38.0	35.0	40.0	27.0	41.0
49	35.48253577683331	38.0	34.0	40.0	27.0	41.0
50	35.28832335819389	38.0	34.0	40.0	26.0	41.0
51	33.42987576995892	36.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	14.0
10	10.0
11	9.0
12	9.0
13	6.0
14	8.0
15	13.0
16	15.0
17	41.0
18	105.0
19	182.0
20	314.0
21	536.0
22	982.0
23	1475.0
24	2284.0
25	2969.0
26	3842.0
27	4451.0
28	4926.0
29	5773.0
30	7341.0
31	9790.0
32	12600.0
33	18780.0
34	25766.0
35	33667.0
36	42748.0
37	70547.0
38	71234.0
39	46135.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.19729498224116	18.253959593865428	23.21415906028185	16.334586363611564
2	31.122435061569437	24.29305632089214	26.801390154346414	17.783118463192007
3	31.421416451614665	24.458914610260297	28.147897582506314	15.971771355618722
4	27.390077964307736	26.331094610151183	27.69915270419938	18.579674721341707
5	21.963947645521554	37.428596369667574	23.939243489789348	16.66821249502152
6	25.379046205718836	35.69472254199652	25.900354085624343	13.025877166660301
7	49.36984761769664	42.57156730627588	5.516424880925752	2.5421601951017245
8	84.95026979251347	4.162279242071264	7.7934300476297	3.0940209177855738
9	78.93245093813594	6.919127716338679	9.785093486243037	4.363327859282336
10	39.62076283901379	28.764683096094146	18.170757655942253	13.443796408949801
11	29.463033788170595	25.497165678245832	27.1912116930094	17.848588840574177
12	25.926815029816304	24.7731724216947	29.48922193912346	19.810790609365537
13	22.90590270010748	27.12546852238814	31.961819858256636	18.006808919247746
14	20.942609758359747	28.292205205986175	30.95548559924491	19.80969943640917
15	19.932729187239822	25.814969801788436	35.42793075416419	18.824370256807555
16	22.638292532557873	25.71212675065061	32.57451347325808	19.075067243533436
17	23.1265924305332	24.71888656711532	30.514924518110742	21.639596484240734
18	23.973888231154078	24.155841321628685	32.11594803834382	19.754322408873417
19	23.583793899252	26.021474283781348	29.344095935926322	21.050635881040325
20	25.248105450954505	26.559968137749674	29.246163163092163	18.945763248203658
21	23.04666401147914	27.24740710026243	31.00186044989061	18.704068438367823
22	23.592523282902956	24.742619578916354	29.684269105074502	21.980588033106187
23	21.277872649204262	26.744921953854295	30.75962005357659	21.21758534336485
24	21.21185668534391	24.905204349415406	32.61243173349192	21.270507231748766
25	20.810305037399953	27.48473721827278	30.78853613692038	20.916421607406882
26	20.775387502796132	27.74825548723601	30.00643792044258	21.469919089525284
27	20.361560159093017	27.65659695890097	31.163354047433288	20.818488834572722
28	19.05924523566608	27.1802999634457	32.298446715296606	21.46200808559161
29	20.571338159955044	26.371740802775946	31.599277643502884	21.45764339376613
30	20.944246517794305	26.364102592081355	31.18681426599523	21.50483662412911
31	23.045027252044587	26.709458832772288	29.06530124557393	21.180212669609197
32	23.06057646667285	26.62871203400095	29.926236708149425	20.38447479117678
33	22.418693975088523	25.656476929875772	29.867040575266383	22.057788519769325
34	20.18397176044389	26.756379269896176	31.206182585970787	21.853466383689145
35	20.7371964493232	27.242769615197858	30.64477409991871	21.375259835560236
36	23.018839101091718	27.564938430565938	28.72921997501214	20.687002493330205
37	21.64696190169623	27.894199870150416	29.875497165678244	20.583341062475107
38	20.969343495790802	28.792235213242474	28.271472919815153	21.96694837115157
39	20.885868764628537	26.721734528531442	30.083092820627535	22.309303886212483
40	23.102859418732166	25.283295778797417	29.86758616174457	21.74625864072585
41	19.788148770520873	26.059665337254284	30.486281228006046	23.6659046642188
42	21.557485719273934	26.682452302102146	29.068029177964856	22.692032800659067
43	21.124835642073446	26.380742979665996	30.120465494383186	22.373955883877375
44	21.058274091734912	26.415660514269813	30.02962534576543	22.496440048229847
45	21.176939150740086	27.08154881089427	28.328486706785462	23.41302533158018
46	20.79830213487989	27.614041213602565	29.065574038813025	22.522082612704526
47	20.44994516855894	27.199668283421264	30.514378931632557	21.836007616387235
48	21.47482936782895	25.951639214573706	30.83327422813153	21.740257189465815
49	21.31360856352536	25.593461691645437	30.906928402686468	22.186001342142735
50	20.13459618416817	27.08973260806704	30.208304917370928	22.56736629039386
51	20.690548805438404	26.065666788514314	28.8342453720627	24.409539033984583
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	162.0
1	507.0
2	852.0
3	2446.5
4	4041.0
5	2775.0
6	1509.0
7	1382.5
8	1256.0
9	1203.0
10	1150.0
11	1193.5
12	1237.0
13	1309.5
14	1382.0
15	1296.0
16	1210.0
17	1202.5
18	1195.0
19	1319.0
20	1443.0
21	1609.5
22	1776.0
23	2051.0
24	2326.0
25	2667.5
26	3622.0
27	4235.0
28	5183.0
29	6131.0
30	6994.5
31	7858.0
32	9188.5
33	10519.0
34	11990.0
35	13461.0
36	14491.5
37	15522.0
38	16606.5
39	17691.0
40	19225.0
41	20759.0
42	22829.5
43	24900.0
44	26579.0
45	28258.0
46	29452.5
47	30647.0
48	30344.5
49	30042.0
50	29367.5
51	28693.0
52	26177.0
53	23661.0
54	21386.0
55	19111.0
56	17174.0
57	15237.0
58	13834.5
59	12432.0
60	11497.0
61	10562.0
62	9469.0
63	8376.0
64	7061.0
65	5746.0
66	4791.0
67	3836.0
68	3218.5
69	2601.0
70	2102.5
71	1604.0
72	1269.0
73	934.0
74	731.5
75	420.5
76	312.0
77	239.0
78	166.0
79	125.0
80	84.0
81	67.0
82	50.0
83	39.5
84	29.0
85	20.5
86	12.0
87	13.5
88	15.0
89	12.5
90	10.0
91	5.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	2.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	366578.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.14841351135971
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.86021118690785	31.72514318493245
2	8.965760370923487	7.916481925985838
3	4.4224463877645475	5.857319755765449
4	2.974308330727359	5.252439763809339
5	2.2418582241913945	4.948724195772213
6	1.7910786670429848	4.744396897439319
7	1.4592905580538496	4.509775409312896
8	1.1769311524344765	4.156771455366272
9	0.9882951081389454	3.926849499483491
>10	4.095302031850491	25.38924384921898
>50	0.017602653718223987	0.5539821262706625
>100	0.005657995838000567	0.43019654094164667
>500	6.286662042222853E-4	0.21870637049162425
>1k	6.286662042222853E-4	0.3699690252098161
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1333	0.363633387710119	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	788	0.21496107240478154	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04228295205931616	0.0
2	0.0	0.0	0.0	0.23078308027213854	0.0
3	0.0	0.0	0.0	0.2976174238497673	0.0
4	0.0	0.0	0.0	0.3876391927502469	0.0
5	0.0	0.0	0.0	0.6675250560590106	0.0
6	0.0	0.0	0.0	0.9624145475178543	0.0
7	0.0	0.0	0.0	1.2292063353501848	0.0
8	0.0	0.0	0.0	1.9035512223865043	0.0
9	0.0	0.0	0.0	2.138426201245028	0.0
10	0.0	0.0	0.0	2.7159294884035594	0.0
11	0.0	0.0	0.0	3.38809202952714	0.0
12	0.0	0.0	0.0	3.960139451903824	0.0
13	0.0	0.0	0.0	4.2004702955441955	0.0
14	0.0	0.0	0.0	4.304131726399293	0.0
15	0.0	0.0	0.0	4.480083365613867	0.0
16	0.0	0.0	0.0	4.872360043428683	0.0
17	0.0	0.0	0.0	5.397487028681481	0.0
18	0.0	0.0	0.0	5.976627075274567	0.0
19	0.0	0.0	0.0	6.320073763291851	0.0
20	0.0	0.0	0.0	6.657519000049103	0.0
21	0.0	0.0	0.0	7.103263152726023	0.0
22	0.0	0.0	0.0	7.578196181985826	0.0
23	0.0	0.0	0.0	8.050674072093797	0.0
24	0.0	0.0	0.0	8.432311813584013	0.0
25	0.0	0.0	0.0	8.78421509201316	0.0
26	0.0	0.0	0.0	9.109657426250347	0.0
27	0.0	0.0	0.0	9.44955780215943	0.0
28	0.0	0.0	0.0	9.775272929635712	0.0
29	0.0	0.0	0.0	10.15854743056048	0.0
30	0.0	0.0	0.0	10.61574889927928	0.0
31	0.0	0.0	0.0	10.980746253184861	0.0
32	0.0	0.0	0.0	11.34792595300318	0.0
33	0.0	0.0	0.0	11.733655593079781	0.0
34	0.0	0.0	0.0	12.112838195418165	0.0
35	0.0	0.0	0.0	12.523937606730355	0.0
36	0.0	0.0	0.0	12.901756242873276	0.0
37	0.0	0.0	0.0	13.291850574775355	0.0
38	0.0	0.0	0.0	13.681944906677433	0.0
39	0.0	0.0	0.0	14.136145649766217	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACAG	25	3.885589E-5	45.000004	2
AGCGTAA	20	7.026994E-4	45.0	1
CGCGAAG	20	7.026994E-4	45.0	2
TCGATAG	20	7.026994E-4	45.0	2
TTCATCG	40	6.7939254E-9	45.0	15
CGTCATA	20	7.026994E-4	45.0	38
TATAACG	20	7.026994E-4	45.0	1
AATACCG	20	7.026994E-4	45.0	21
CAGACGA	20	7.026994E-4	45.0	2
ACGGGTC	30	2.1614414E-6	44.999996	5
GCGATCA	30	2.1614414E-6	44.999996	9
CGTTTTA	310	0.0	43.54839	1
CGCAGGA	70	0.0	41.785713	4
TACGAAT	65	0.0	41.538464	12
CGAATAT	65	0.0	41.538464	14
GCTACGA	65	0.0	41.538464	10
GTATAAG	55	6.002665E-11	40.909092	2
CACAACG	105	0.0	40.714287	12
TCGAAAG	50	1.0768417E-9	40.500004	2
TACGGGA	130	0.0	39.807693	4
>>END_MODULE