##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934702.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	436127
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02798267477134	33.0	31.0	34.0	30.0	34.0
2	32.21411194445655	33.0	31.0	34.0	30.0	34.0
3	31.851089246939537	33.0	31.0	34.0	30.0	34.0
4	35.738711430386104	37.0	35.0	37.0	35.0	37.0
5	32.081265319505555	37.0	35.0	37.0	0.0	37.0
6	33.995583855161456	37.0	35.0	37.0	19.0	37.0
7	20.74201551382969	32.0	0.0	37.0	0.0	37.0
8	28.128476338314297	35.0	17.0	37.0	17.0	37.0
9	34.97317983064566	37.0	32.0	39.0	32.0	39.0
10	36.394552504201755	37.0	35.0	39.0	32.0	39.0
11	36.79877191735435	39.0	37.0	39.0	33.0	39.0
12	37.13475661905821	39.0	37.0	39.0	34.0	39.0
13	36.95429542312216	39.0	37.0	39.0	33.0	39.0
14	38.319968724706335	40.0	38.0	41.0	34.0	41.0
15	38.47946125784462	40.0	38.0	41.0	34.0	41.0
16	38.553843261251885	40.0	38.0	41.0	34.0	41.0
17	38.51717962886957	40.0	38.0	41.0	34.0	41.0
18	38.38529144033733	40.0	38.0	41.0	34.0	41.0
19	38.29155268992748	40.0	37.0	41.0	34.0	41.0
20	38.207441868996874	40.0	37.0	41.0	34.0	41.0
21	38.096061468333765	40.0	37.0	41.0	34.0	41.0
22	38.071330140073876	40.0	36.0	41.0	34.0	41.0
23	37.96940111481289	40.0	36.0	41.0	34.0	41.0
24	37.821735411932764	40.0	35.0	41.0	33.0	41.0
25	37.65870721143153	40.0	35.0	41.0	33.0	41.0
26	37.520281936225	39.0	35.0	41.0	33.0	41.0
27	37.47428845267548	39.0	35.0	41.0	33.0	41.0
28	37.377096579666016	39.0	35.0	41.0	33.0	41.0
29	37.33732605410809	39.0	35.0	41.0	33.0	41.0
30	37.16686882490651	39.0	35.0	41.0	33.0	41.0
31	36.99432734043065	39.0	35.0	41.0	32.0	41.0
32	36.83176689358834	39.0	35.0	41.0	31.0	41.0
33	36.611649817599	39.0	35.0	41.0	31.0	41.0
34	36.32229602845043	39.0	35.0	41.0	30.0	41.0
35	36.04828868655231	39.0	35.0	41.0	29.0	41.0
36	35.83258316958134	39.0	35.0	41.0	27.0	41.0
37	35.72158339199362	39.0	35.0	41.0	27.0	41.0
38	35.566080522416634	39.0	35.0	40.0	26.0	41.0
39	35.41685334776338	39.0	35.0	40.0	25.0	41.0
40	35.2594955139214	38.0	35.0	40.0	24.0	41.0
41	35.09571523890977	38.0	34.0	40.0	23.0	41.0
42	34.968222559025236	38.0	34.0	40.0	23.0	41.0
43	34.86791462119979	38.0	34.0	40.0	23.0	41.0
44	34.69124360564698	38.0	34.0	40.0	22.0	41.0
45	34.55143799856464	38.0	34.0	40.0	23.0	41.0
46	34.39504777278178	38.0	33.0	40.0	22.0	41.0
47	34.32524700373973	38.0	33.0	40.0	22.0	41.0
48	34.19035739589615	37.0	33.0	40.0	21.0	41.0
49	34.07549865062241	37.0	33.0	40.0	20.0	41.0
50	33.924123019212296	37.0	33.0	40.0	20.0	41.0
51	32.095254363981134	35.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	18.0
10	25.0
11	12.0
12	11.0
13	12.0
14	19.0
15	19.0
16	45.0
17	69.0
18	162.0
19	279.0
20	513.0
21	965.0
22	1583.0
23	2683.0
24	4115.0
25	5850.0
26	7193.0
27	7616.0
28	7889.0
29	8277.0
30	9423.0
31	11480.0
32	15302.0
33	22409.0
34	30260.0
35	38681.0
36	49710.0
37	80352.0
38	80275.0
39	50867.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.469360988886265	18.01837538148292	21.757194578643375	22.75506905098744
2	34.11643856032761	23.811871312713954	24.76939056742646	17.30229955953197
3	30.279482811199486	22.64500936653773	30.746319306073687	16.32918851618909
4	26.78783932203234	24.53390870090593	30.20129457703834	18.476957400023387
5	21.914717502012028	35.61737750701057	26.173797999206656	16.294106991770747
6	26.082081595498558	31.921435728583646	28.4297922394165	13.566690436501293
7	47.03744551472393	42.78318012872398	7.839918188967893	2.339456167584213
8	82.62226369841812	3.5308522517523566	11.510408665365821	2.3364753844636996
9	77.26946508700448	5.87993864172592	13.054683612800858	3.795912658468749
10	40.97063470044276	26.626418451506094	20.050581596645014	12.352365251406127
11	30.254260800179765	24.578161865695083	28.100988932122984	17.066588402002168
12	26.500537687416738	22.322396916494508	32.36327950344739	18.813785892641363
13	23.606197277398554	23.051771616983128	33.9724438065059	19.369587299112414
14	20.57313580677188	23.890976710912188	34.95908301939573	20.576804462920204
15	20.537366409325724	23.433999729436607	35.640306607937596	20.38832725330007
16	24.599715220566488	23.758905089572533	32.97021280498571	18.671166884875277
17	24.523590605488767	23.437439094575662	31.520176462360734	20.518793837574833
18	24.78796313917735	23.126749777014492	32.16769427253988	19.91759281126828
19	24.09802649228321	25.56961618977958	30.332219743331645	20.00013757460556
20	25.73218351535218	25.260073327264763	30.70871558055337	18.299027576829687
21	25.50770761727662	25.26741063956141	31.180825768640787	18.044055974521182
22	25.5579223483068	22.30588796382705	31.854024171858196	20.282165516007954
23	23.326920828107408	24.433479238845564	32.34447764068723	19.895122292359794
24	22.792902067517034	23.76830602095261	33.55788566174532	19.880906249785042
25	23.222822709898722	24.580225484778516	31.870074542507115	20.32687726281565
26	22.53701330117145	25.30203358196122	30.76076463965771	21.40018847720962
27	21.843637289138258	25.031928773040878	32.06061537121067	21.063818566610184
28	20.348889199705592	25.89750233303602	32.62833991016378	21.125268557094607
29	21.977313947542804	25.407507446225523	31.60295051670729	21.01222808952438
30	22.866275190483506	24.43668931297535	32.23143717311701	20.46559832342414
31	23.68736629468022	24.355520295693687	31.664171216182442	20.292942193443654
32	23.957700394609827	24.640299729207317	31.11570712200804	20.286292754174816
33	23.57937022931394	24.75563310687025	30.3464357859064	21.318560877909416
34	21.726698874410435	25.710171578462237	32.18672542630931	20.376404120818016
35	22.433144473972032	26.50993861879682	31.237919229949075	19.818997677282077
36	23.517461656810976	27.197123773579712	29.22497345956568	20.060441110043634
37	22.752317558876197	27.257885891036327	29.227495660667653	20.762300889419823
38	23.08364306727169	26.787610031023075	29.426061674695674	20.702685227009564
39	23.13523354435749	25.942901952871527	28.989491593045148	21.932372909725835
40	24.89504204050655	25.359814916297317	28.98651080992463	20.758632233271502
41	22.060317292898628	25.65858110137643	29.547815200618167	22.73328640510677
42	22.42443141561976	25.657434646330085	29.390292277249518	22.527841660800636
43	21.921596232290135	25.21467370742926	30.21092479942769	22.65280526085292
44	22.418469849378734	26.007562017485732	29.647098207632183	21.92686992550335
45	22.701644245827477	25.52375798792553	29.082813033818134	22.691784732428857
46	22.467308834353297	26.232955079598376	28.929875930634886	22.369860155413445
47	21.793193267098804	26.14169725790882	30.55073407516618	21.514375399826196
48	22.133690415865104	26.59959140342148	30.154289920137938	21.112428260575474
49	22.363898589172422	26.06511406081256	29.847727840743637	21.72325950927138
50	21.72050801716014	26.326735102389897	30.285902959459055	21.666853920990903
51	21.842261543082632	25.95597154039993	29.2756467726144	22.926120143903038
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	259.0
1	751.5
2	1244.0
3	6590.0
4	11936.0
5	7939.5
6	3943.0
7	3473.0
8	3003.0
9	2907.5
10	2812.0
11	2814.0
12	2816.0
13	2767.0
14	2718.0
15	2556.5
16	2395.0
17	2343.0
18	2291.0
19	2264.0
20	2237.0
21	2341.0
22	2445.0
23	2623.5
24	2802.0
25	3203.0
26	4132.0
27	4660.0
28	5437.5
29	6215.0
30	7154.5
31	8094.0
32	9112.0
33	10130.0
34	11530.5
35	12931.0
36	13881.5
37	14832.0
38	15906.5
39	16981.0
40	18206.0
41	19431.0
42	20756.0
43	22081.0
44	23413.0
45	24745.0
46	26119.5
47	27494.0
48	27888.5
49	28283.0
50	28295.0
51	28307.0
52	27714.0
53	27121.0
54	26084.5
55	25048.0
56	24111.5
57	23175.0
58	22333.5
59	21492.0
60	19985.0
61	18478.0
62	16684.0
63	14890.0
64	13388.5
65	11887.0
66	10237.0
67	8587.0
68	7331.0
69	6075.0
70	5052.0
71	4029.0
72	3303.0
73	2577.0
74	2191.0
75	1442.5
76	1080.0
77	810.5
78	541.0
79	445.0
80	349.0
81	252.0
82	155.0
83	118.5
84	82.0
85	58.0
86	34.0
87	25.0
88	16.0
89	10.5
90	5.0
91	5.0
92	5.0
93	4.5
94	4.0
95	2.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	436127.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.74976584895417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.17982178095626	34.699996449919055
2	10.32564708844672	10.06745755601426
3	5.379879167802466	7.8680354917811
4	3.4571622485856346	6.741434004815742
5	2.4709167999409676	6.022830771468465
6	1.8225782149428906	5.331015673192248
7	1.344185541980925	4.587011130038375
8	0.9946724145796473	3.8792037845737335
9	0.7255209013584697	3.1832076653772816
>10	2.2750912821251084	14.78631481451462
>50	0.018018043553361108	0.5741159239653333
>100	0.005005012098135122	0.39990480211478757
>500	5.005012098135123E-4	0.22837760511253274
>1k	0.0010010024196270245	1.63109432711248
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4250	0.9744867893985009	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2435	0.5583236075730235	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	936	0.2146163846769404	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007795894315188007	0.0
2	0.0	0.0	0.0	0.04792182093747922	0.0
3	0.0	0.0	0.0	0.0846083824207169	0.0
4	0.0	0.0	0.0	0.12358785399665694	0.0
5	0.0	0.0	0.0	0.25588876634558283	0.0
6	0.0	0.0	0.0	0.43565291761344743	0.0
7	0.0	0.0	0.0	0.5924879679542886	0.0
8	0.0	0.0	0.0	0.8901076979870542	0.0
9	0.0	0.0	0.0	1.0806485266906198	0.0
10	0.0	0.0	0.0	1.3920257172795998	0.0
11	0.0	0.0	0.0	1.7045493629149306	0.0
12	0.0	0.0	0.0	1.9654825314644588	0.0
13	0.0	0.0	0.0	2.101681390970979	0.0
14	0.0	0.0	0.0	2.1849140273360743	0.0
15	0.0	0.0	0.0	2.26814666370117	0.0
16	0.0	0.0	0.0	2.397237501920312	0.0
17	0.0	0.0	0.0	2.543295874825452	0.0
18	0.0	0.0	0.0	2.7207671160006144	0.0
19	0.0	0.0	0.0	2.8386226947655158	0.0
20	0.0	0.0	0.0	2.961751966743632	0.0
21	0.0	0.0	0.0	3.0933650060647473	0.0
22	0.0	0.0	0.0	3.253868712553912	0.0
23	0.0	0.0	0.0	3.421480440330454	0.0
24	0.0	0.0	0.0	3.555615680753542	0.0
25	0.0	0.0	0.0	3.684477227963414	0.0
26	0.0	0.0	0.0	3.811963029117665	0.0
27	0.0	0.0	0.0	3.959397148078427	0.0
28	0.0	0.0	0.0	4.093532388501514	0.0
29	0.0	0.0	0.0	4.253348221962868	0.0
30	0.0	0.0	0.0	4.43930323048103	0.0
31	0.0	0.0	0.0	4.6135643975264085	0.0
32	0.0	0.0	0.0	4.80960821045246	0.0
33	0.0	0.0	0.0	4.997397547044783	0.0
34	0.0	0.0	0.0	5.169824386016	0.0
35	0.0	0.0	0.0	5.358530886645403	0.0
36	0.0	0.0	0.0	5.535314254792755	0.0
37	0.0	0.0	0.0	5.723562173403619	0.0
38	0.0	0.0	0.0	5.934280610922965	0.0
39	0.0	0.0	0.0	6.229378139853758	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTAAG	25	3.88681E-5	45.000004	2
TAGCACG	20	7.028468E-4	45.0	1
GCGTATA	20	7.028468E-4	45.0	1
CGAAGCT	20	7.028468E-4	45.0	39
CGTTTTA	790	0.0	44.14557	1
CGTTATT	385	0.0	43.24675	1
AGGCGAT	235	0.0	43.085106	7
TCAAGCG	265	0.0	42.45283	17
CGTTTTT	1530	0.0	41.911762	1
CGACCAA	275	0.0	41.727272	29
ACACGTG	245	0.0	41.32653	42
TACGAAT	60	3.6379788E-12	41.249996	12
ACGACCA	280	0.0	40.98214	28
AAACACG	275	0.0	40.909092	40
TTACACG	450	0.0	40.5	34
CGAGACA	285	0.0	40.263157	22
CGAAGGA	95	0.0	40.263157	4
TACACGC	460	0.0	40.108692	35
AGTACGG	45	1.9235813E-8	40.0	2
AACACGT	260	0.0	39.807693	41
>>END_MODULE