##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934701.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	254666
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59135102447912	31.0	31.0	34.0	30.0	34.0
2	31.762536027581223	31.0	31.0	34.0	30.0	34.0
3	31.366036298524342	31.0	31.0	34.0	28.0	34.0
4	35.33797601564402	37.0	35.0	37.0	33.0	37.0
5	31.68863138385179	35.0	35.0	37.0	0.0	37.0
6	33.6833656632609	37.0	35.0	37.0	17.0	37.0
7	21.083945245929964	32.0	0.0	37.0	0.0	37.0
8	28.295665695459935	35.0	17.0	37.0	17.0	37.0
9	34.86624834096424	37.0	32.0	39.0	32.0	39.0
10	36.13246369754895	37.0	35.0	39.0	32.0	39.0
11	36.50916887216982	38.0	35.0	39.0	32.0	39.0
12	36.90629687512271	39.0	35.0	39.0	33.0	39.0
13	36.627237244076554	39.0	35.0	39.0	33.0	39.0
14	37.92595006793211	40.0	37.0	41.0	33.0	41.0
15	38.1203419380679	40.0	37.0	41.0	33.0	41.0
16	38.22141157437585	40.0	37.0	41.0	34.0	41.0
17	38.21110395576952	40.0	37.0	41.0	34.0	41.0
18	38.08585362788908	40.0	37.0	41.0	34.0	41.0
19	38.00076570881075	40.0	37.0	41.0	34.0	41.0
20	37.914213126212374	39.0	36.0	41.0	34.0	41.0
21	37.82541839114762	39.0	36.0	41.0	33.0	41.0
22	37.7728161592046	39.0	36.0	41.0	33.0	41.0
23	37.70528456880777	39.0	36.0	41.0	33.0	41.0
24	37.55315589831387	39.0	35.0	41.0	33.0	41.0
25	37.419329631752966	39.0	35.0	41.0	33.0	41.0
26	37.27560412461813	39.0	35.0	40.0	33.0	41.0
27	37.244948285204934	39.0	35.0	40.0	33.0	41.0
28	37.19332380451258	39.0	35.0	40.0	33.0	41.0
29	37.17526093000244	39.0	35.0	40.0	33.0	41.0
30	37.02643855088626	39.0	35.0	40.0	32.0	41.0
31	36.85702056811667	39.0	35.0	40.0	31.0	41.0
32	36.67664705928549	39.0	35.0	40.0	31.0	41.0
33	36.44206529336464	39.0	35.0	40.0	30.0	41.0
34	36.18213660245184	39.0	35.0	40.0	30.0	41.0
35	35.902389796831926	39.0	35.0	40.0	29.0	41.0
36	35.65953052233121	39.0	35.0	40.0	26.0	41.0
37	35.51494899201307	39.0	35.0	40.0	25.0	41.0
38	35.36479545757973	38.0	35.0	40.0	25.0	41.0
39	35.29212380137121	39.0	35.0	40.0	24.0	41.0
40	35.23785271689193	38.0	35.0	40.0	24.0	41.0
41	35.16530278875076	38.0	35.0	40.0	24.0	41.0
42	35.01618983295768	38.0	35.0	40.0	23.0	41.0
43	34.881586077450464	38.0	34.0	40.0	23.0	41.0
44	34.70879112248985	38.0	34.0	40.0	22.0	41.0
45	34.6011520972568	38.0	34.0	40.0	22.0	41.0
46	34.41621574925589	38.0	34.0	40.0	20.0	41.0
47	34.39338584655981	38.0	34.0	40.0	20.0	41.0
48	34.25019044552473	38.0	33.0	40.0	20.0	41.0
49	34.124421791680085	38.0	33.0	40.0	19.0	41.0
50	33.87838973400454	38.0	33.0	40.0	18.0	41.0
51	31.88727980963301	35.0	29.0	39.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	5.0
10	11.0
11	2.0
12	2.0
13	1.0
14	3.0
15	5.0
16	8.0
17	28.0
18	55.0
19	128.0
20	256.0
21	558.0
22	887.0
23	1700.0
24	2605.0
25	3861.0
26	4616.0
27	4782.0
28	4771.0
29	5205.0
30	6068.0
31	7420.0
32	9886.0
33	13679.0
34	18665.0
35	23985.0
36	29064.0
37	44327.0
38	44231.0
39	27843.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.56554074748887	18.896908107089285	24.395875381872727	20.14167576354912
2	32.76369833428883	25.220092199194237	25.668522692467782	16.347686774049148
3	27.828214209984843	23.81158065858811	33.447731538564234	14.912473592862806
4	25.08697666747819	24.47362427650334	33.96841352987835	16.47098552614012
5	20.343508752640712	36.30912646368184	27.526250068717456	15.821114714959986
6	23.31799297903921	33.00676179780576	30.635420511572022	13.039824711583014
7	47.356144911374116	41.055735747999336	9.008269655156164	2.5798496854703807
8	80.50740970526101	3.3278882929012905	12.4684881374035	3.6962138644342
9	75.01747386773265	6.7237087008081176	14.257890727462637	4.000926703996607
10	32.846159283139485	38.112272545216086	19.187484783991582	9.854083387652848
11	24.700980892620137	24.097052610085367	35.73739721831733	15.46456927897717
12	24.634619462354614	21.93775376375331	36.43713726999285	16.990489503899227
13	19.956334964227654	26.256744127602428	38.15428836201142	15.632632546158497
14	16.6331587255464	29.41617648213739	35.120118115492446	18.83054667682376
15	14.928180440262933	27.23685140537017	41.57170568509342	16.263262469273478
16	17.704758389419865	26.503341631784377	37.43216605279072	18.359733926005042
17	18.05776978473766	24.73946266875044	34.4435456637321	22.759221882779798
18	18.6711221757125	24.889463061421626	37.81541312935374	18.62400163351213
19	18.929499815444544	27.23881476129519	34.376791562281575	19.454893860978693
20	19.604108911279873	27.221144557970046	35.297605491113856	17.87714103963623
21	18.950704059434713	27.27336982557546	36.38883871423747	17.387087400752357
22	18.51012698986123	24.572577415124123	34.721556862714294	22.195738732300345
23	16.21889062536813	26.989075887633213	34.924567865360906	21.867465621637752
24	17.639967643894355	25.505564150691495	37.157688894473544	19.696779310940606
25	15.840355603025138	28.5943156919259	35.611742439116334	19.953586265932632
26	16.346116089309135	30.15439830994322	34.17927795622502	19.32020764452263
27	16.670462488121697	29.376516692452075	34.73490768300441	19.218113136421824
28	14.952133382548121	27.49758507221223	37.46750645944099	20.082775085798655
29	15.580014607368081	26.662766132895637	36.93465166139178	20.8225675983445
30	17.476616430933063	27.863947287820125	35.094987159652256	19.56444912159456
31	19.037091720135393	28.37638318424917	33.38647483370376	19.20005026191168
32	18.867457768214052	28.769447040437278	33.54511399244501	18.817981198903663
33	18.131984638703244	27.503867811172277	32.670635263443096	21.69351228668138
34	16.911955266898605	27.17952141235972	34.50087565674256	21.40764766399912
35	15.494412289037406	27.693527993528775	34.594724069958296	22.217335647475515
36	18.67740491467255	28.321016547163737	32.50414268100179	20.497435857161932
37	17.15933811345056	27.77755962711944	35.98085335301925	19.08224890641075
38	16.774913023332523	28.23659224238807	33.30754792551813	21.680946808761277
39	17.455412186942898	26.15857633135165	34.621818381723514	21.764193099981938
40	18.491671444166084	26.08632483331108	33.8993034013178	21.52270032120503
41	16.627661328956357	26.521404506294523	32.92115947947508	23.929774685274044
42	18.357377898895024	26.952557467427923	32.41618433556109	22.273880298115962
43	18.734342236498	27.104128544839124	31.373249668192848	22.78827955047003
44	18.27609496359938	27.444181791051808	31.277045227867088	23.00267801748172
45	17.750308246880227	27.6958840206388	31.090919086175617	23.46288864630536
46	19.118374655431037	28.28253477103343	31.026913683020112	21.572176890515422
47	16.608027769706204	27.687245254568733	34.24681740004555	21.45790957567952
48	17.98591095788209	26.045879701255764	33.124955824491686	22.843253516370464
49	19.124264723206082	24.28239340940683	33.74184225613156	22.851499611255527
50	17.866931588826148	25.117605019908428	33.11003431946157	23.90542907180385
51	17.119285652580242	25.59705653679722	31.214610509451596	26.069047301170944
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	153.0
1	290.0
2	427.0
3	3526.0
4	6625.0
5	4600.0
6	2575.0
7	2364.5
8	2154.0
9	2043.5
10	1933.0
11	1906.5
12	1880.0
13	1860.0
14	1840.0
15	1783.5
16	1727.0
17	1659.0
18	1591.0
19	1566.5
20	1542.0
21	1593.0
22	1644.0
23	1676.5
24	1709.0
25	2072.0
26	2854.0
27	3273.0
28	3746.0
29	4219.0
30	4884.0
31	5549.0
32	6512.0
33	7475.0
34	8820.0
35	10165.0
36	11094.0
37	12023.0
38	13123.5
39	14224.0
40	16539.5
41	18855.0
42	21723.5
43	24592.0
44	26716.0
45	28840.0
46	28025.0
47	27210.0
48	24847.5
49	22485.0
50	19054.5
51	15624.0
52	12932.0
53	10240.0
54	8548.0
55	6856.0
56	5987.5
57	5119.0
58	4462.0
59	3805.0
60	3055.5
61	2306.0
62	1917.0
63	1528.0
64	1203.0
65	878.0
66	697.0
67	516.0
68	408.5
69	301.0
70	222.5
71	144.0
72	125.0
73	106.0
74	83.0
75	42.0
76	24.0
77	14.0
78	4.0
79	5.0
80	6.0
81	3.5
82	1.0
83	2.0
84	3.0
85	1.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	254666.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.10088674070837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.17596588766087	43.07565042221482
2	9.184368690066263	10.121337179524984
3	3.7363694926931457	6.17631816714969
4	2.2024357434666952	4.854246498177848
5	1.5422462099340017	4.248956686993009
6	1.1161540104995136	3.6900645430632673
7	0.9049114296898451	3.4902995538398853
8	0.6744784778115618	2.97314897719522
9	0.48746112603472275	2.4173586266523683
>10	1.9482899088760168	15.368018955654605
>50	0.011502746638885742	0.411581368629252
>100	0.01365951163367682	1.3409075387969374
>500	7.189216649303589E-4	0.2467903682926121
>1k	0.0014378433298607178	1.5853211138154633
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2582	1.0138769996780097	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1420	0.557593082704405	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	623	0.24463414825693258	No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	267	0.10484320639582827	Illumina PCR Primer Index 7 (95% over 23bp)
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	265	0.10405786402582205	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13154484697603921	0.0
2	0.0	0.0	0.0	0.5760486283995507	0.0
3	0.0	0.0	0.0	0.7668868243110584	0.0
4	0.0	0.0	0.0	1.0244791216730933	0.0
5	0.0	0.0	0.0	1.71518773609355	0.0
6	0.0	0.0	0.0	2.1836444598022506	0.0
7	0.0	0.0	0.0	2.737703501841628	0.0
8	0.0	0.0	0.0	4.0586493681920635	0.0
9	0.0	0.0	0.0	4.496870410655525	0.0
10	0.0	0.0	0.0	5.799360731310815	0.0
11	0.0	0.0	0.0	7.300150000392671	0.0
12	0.0	0.0	0.0	8.58732614483284	0.0
13	0.0	0.0	0.0	9.071882387126667	0.0
14	0.0	0.0	0.0	9.272930033848256	0.0
15	0.0	0.0	0.0	9.627119442721055	0.0
16	0.0	0.0	0.0	10.366911955266898	0.0
17	0.0	0.0	0.0	11.406705253155113	0.0
18	0.0	0.0	0.0	12.540739635444071	0.0
19	0.0	0.0	0.0	13.209851334689358	0.0
20	0.0	0.0	0.0	13.873072966159597	0.0
21	0.0	0.0	0.0	14.731059505391375	0.0
22	0.0	0.0	0.0	15.664831583328752	0.0
23	0.0	0.0	0.0	16.717190359137067	0.0
24	0.0	0.0	0.0	17.386302058382352	0.0
25	0.0	0.0	0.0	18.018109995052342	0.0
26	0.0	0.0	0.0	18.599656020041937	0.0
27	0.0	0.0	0.0	19.20005026191168	0.0
28	0.0	0.0	0.0	19.81536600881154	0.0
29	0.0	0.0	0.0	20.508037979157013	0.0
30	0.0	0.0	0.0	21.229374946007713	0.0
31	0.0	0.0	0.0	21.923224929908194	0.0
32	0.0	0.0	0.0	22.5440380733981	0.0
33	0.0	0.0	0.0	23.160139162667964	0.0
34	0.0	0.0	0.0	23.784093675637894	0.0
35	0.0	0.0	0.0	24.47087557820832	0.0
36	0.0	0.0	0.0	25.117997691093432	0.0
37	0.0	0.0	0.0	25.756481037908475	0.0
38	0.0	0.0	0.0	26.386718289838456	0.0
39	0.0	0.0	0.0	27.07350019240888	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGGC	20	7.0229336E-4	45.000004	4
TTTACGG	20	7.0229336E-4	45.000004	2
CGGTTTA	20	7.0229336E-4	45.000004	1
TGAACGG	40	6.7793735E-9	45.000004	2
GTTAGCG	20	7.0229336E-4	45.000004	1
CCGCTTT	20	7.0229336E-4	45.000004	14
AGGCGAC	20	7.0229336E-4	45.000004	7
GTGGCCG	30	2.1588257E-6	45.000004	9
GCCGCTT	20	7.0229336E-4	45.000004	13
GTTACGG	40	6.7793735E-9	45.000004	2
AAGCCGC	20	7.0229336E-4	45.000004	11
TGCGCAA	20	7.0229336E-4	45.000004	1
GCGGGTA	20	7.0229336E-4	45.000004	5
TTGCGAT	20	7.0229336E-4	45.000004	32
GCGATTC	30	2.1588257E-6	45.000004	9
CGCAGGA	30	2.1588257E-6	45.000004	4
TAGGCGA	20	7.0229336E-4	45.000004	6
GCGGAAG	20	7.0229336E-4	45.000004	1
ATCGGTT	20	7.0229336E-4	45.000004	11
CACCGGG	30	2.1588257E-6	45.000004	3
>>END_MODULE