##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934700.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	674150
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88407921085812	31.0	31.0	34.0	30.0	34.0
2	32.064119261292	33.0	31.0	34.0	30.0	34.0
3	31.59608544092561	33.0	31.0	34.0	28.0	34.0
4	35.56009048431358	37.0	35.0	37.0	33.0	37.0
5	31.935279982199805	37.0	35.0	37.0	0.0	37.0
6	33.861670251427725	37.0	35.0	37.0	18.0	37.0
7	20.97890083809241	32.0	0.0	37.0	0.0	37.0
8	28.20702514277238	35.0	17.0	37.0	17.0	37.0
9	34.884097011050955	37.0	32.0	39.0	32.0	39.0
10	36.31563746940592	37.0	35.0	39.0	32.0	39.0
11	36.734686642438625	38.0	37.0	39.0	33.0	39.0
12	37.07377438255581	39.0	37.0	39.0	34.0	39.0
13	36.88495883705407	39.0	37.0	39.0	33.0	39.0
14	38.255558851887564	40.0	38.0	41.0	34.0	41.0
15	38.415177631091005	40.0	38.0	41.0	34.0	41.0
16	38.47047096343544	40.0	38.0	41.0	34.0	41.0
17	38.43222279908032	40.0	38.0	41.0	34.0	41.0
18	38.272670770600016	40.0	38.0	41.0	34.0	41.0
19	38.15287102276941	40.0	37.0	41.0	34.0	41.0
20	38.054987762367425	40.0	37.0	41.0	34.0	41.0
21	37.94503893792183	40.0	36.0	41.0	33.0	41.0
22	37.88609508269673	40.0	36.0	41.0	33.0	41.0
23	37.7839175257732	39.0	36.0	41.0	33.0	41.0
24	37.625860713491065	39.0	35.0	41.0	33.0	41.0
25	37.47157754209004	39.0	35.0	41.0	33.0	41.0
26	37.33051991396574	39.0	35.0	41.0	33.0	41.0
27	37.28855892605503	39.0	35.0	41.0	33.0	41.0
28	37.17499814581325	39.0	35.0	41.0	33.0	41.0
29	37.14064822368909	39.0	35.0	41.0	33.0	41.0
30	36.94445301490766	39.0	35.0	41.0	32.0	41.0
31	36.77480827708967	39.0	35.0	41.0	31.0	41.0
32	36.597475339316176	39.0	35.0	41.0	31.0	41.0
33	36.3077371504858	39.0	35.0	41.0	30.0	41.0
34	35.97552176815249	39.0	35.0	41.0	28.0	41.0
35	35.690080842542464	39.0	35.0	41.0	26.0	41.0
36	35.45004524215679	39.0	35.0	40.0	25.0	41.0
37	35.32967588815546	39.0	35.0	40.0	24.0	41.0
38	35.158058295631534	39.0	34.0	40.0	23.0	41.0
39	35.03610620781725	38.0	34.0	40.0	23.0	41.0
40	34.91609730772083	38.0	34.0	40.0	23.0	41.0
41	34.75363494771193	38.0	34.0	40.0	22.0	41.0
42	34.63409923607506	38.0	34.0	40.0	21.0	41.0
43	34.54925906697323	38.0	34.0	40.0	21.0	41.0
44	34.40005340057851	38.0	34.0	40.0	20.0	41.0
45	34.31259215308166	38.0	33.0	40.0	20.0	41.0
46	34.1965319290959	38.0	33.0	40.0	20.0	41.0
47	34.1415590002225	38.0	33.0	40.0	20.0	41.0
48	34.0143692056664	38.0	33.0	40.0	20.0	41.0
49	33.90844619150041	37.0	33.0	40.0	20.0	41.0
50	33.75537343321219	37.0	33.0	40.0	19.0	41.0
51	32.01264407031076	35.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	8.0
9	29.0
10	22.0
11	19.0
12	22.0
13	13.0
14	27.0
15	38.0
16	90.0
17	156.0
18	275.0
19	590.0
20	929.0
21	1669.0
22	2767.0
23	4625.0
24	7212.0
25	10507.0
26	13154.0
27	13982.0
28	13954.0
29	14105.0
30	15460.0
31	18899.0
32	24184.0
33	34011.0
34	45493.0
35	57826.0
36	73959.0
37	120240.0
38	120878.0
39	78993.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.33427278795521	17.13135058963139	20.544537565823628	22.98983905658978
2	36.68515908922347	22.66320551805978	23.987243195134614	16.66439219758214
3	30.868946080249206	22.528369057331453	31.280872209448933	15.321812652970406
4	26.277386338352	24.50107542831714	31.51034636208559	17.711191871245273
5	21.346139583178818	35.64280946376919	27.095601869020246	15.915449084031744
6	24.85515093080175	32.60846992509086	29.815916339093672	12.720462805013721
7	46.26062449009864	42.08232589186383	9.384706667655566	2.2723429503819625
8	79.79188607876586	4.05206556404361	13.524883186234518	2.631165170956019
9	74.21998071645775	5.986649855373433	15.556330193577098	4.237039234591708
10	39.450419046206335	25.849291700660093	22.144774901728102	12.555514351405472
11	29.283987243195135	25.04561299414077	29.328191055403103	16.342208707260994
12	26.77490172810205	22.471556775198398	32.853519246458504	17.900022250241044
13	23.543276718831123	22.28005636727731	34.817622190907066	19.3590447229845
14	20.566491136987317	23.356078024178593	35.685233256693614	20.392197582140472
15	20.43877475339316	24.052213898983908	36.258399465994216	19.250611881628718
16	24.167915152414153	23.534376622413408	34.68130238077579	17.61640584439665
17	24.100719424460433	23.772750871467775	33.11073203293036	19.01579767114144
18	23.907884002076692	23.416153674998146	33.49388118371282	19.182081139212343
19	23.568938663502188	24.986427352962988	32.050878884521254	19.393755099013575
20	25.203738040495438	25.215011495957874	32.14818660535489	17.433063858191797
21	25.072164948453608	24.808870429429653	32.594526440703106	17.524438181413633
22	24.213750648965362	22.54602091522658	33.72424534599125	19.515983089816807
23	22.837499072906624	24.519617295854037	33.669806422902916	18.973077208336424
24	22.79626195950456	23.10539197507973	34.363272268782914	19.735073796632797
25	22.521248980197285	24.605354891344653	32.58888971297189	20.284506415486167
26	21.936067640732777	25.31469257583624	32.32411184454499	20.425127938886003
27	21.696803382036638	24.949640287769785	33.08358673885633	20.26996959133724
28	20.875621152562488	25.570570347845432	33.205963064599864	20.347845434992212
29	21.927464214195656	25.09130015575169	32.760364903953125	20.220870726099534
30	22.33746198917155	24.36920566639472	33.25758362382259	20.035748720611142
31	22.825780612623305	24.362975598902324	32.19506044648817	20.616183341986204
32	23.340651190387895	25.065786546020913	31.7103018616035	19.883260401987688
33	23.043832974857228	25.202403026032783	31.35533634947712	20.398427649632872
34	21.257286953942	25.958317881777056	32.61677668174738	20.167618482533562
35	21.332937773492546	26.70177260253653	31.450419046206335	20.514870577764594
36	21.89408885262924	28.09775272565453	29.83149150782467	20.176666913891566
37	22.22368908996514	27.79529778239264	29.680486538604168	20.30052658903805
38	22.028776978417266	27.324779351776314	30.135429800489504	20.51101386931692
39	21.998961655417933	26.59630645998665	29.912037380404954	21.492694504190464
40	23.63717273603797	25.6425127938886	30.329896907216497	20.39041756285693
41	20.887636282726397	26.299191574575392	31.00645256990284	21.806719572795373
42	21.35251798561151	26.1449232366684	30.478083512571384	22.024475265148705
43	22.075057479789365	25.88073870800267	30.611436623896758	21.432767188311207
44	21.911889045464658	26.161833419862045	30.23852258399466	21.68775495067863
45	21.647556181858636	25.89438552250983	29.73596380627457	22.722094489356966
46	21.22405992731588	26.902914781576797	29.613587480531038	22.259437810576284
47	20.767336646147	26.830972335533637	31.015055996439962	21.386635021879403
48	21.33219609879107	25.822443076466662	31.154194170436845	21.691166654305423
49	21.467329229399983	26.06645405325224	31.098568567826153	21.36764814952162
50	20.404509382184973	26.49276867166061	31.512423051249723	21.590298894904695
51	20.608173255210264	25.955796187792036	30.373655714603576	23.062374842394124
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	271.0
1	806.0
2	1341.0
3	12755.0
4	24169.0
5	16170.0
6	8171.0
7	7019.5
8	5868.0
9	5577.5
10	5287.0
11	5063.5
12	4840.0
13	4670.5
14	4501.0
15	4242.0
16	3983.0
17	3824.5
18	3666.0
19	3622.0
20	3578.0
21	3673.0
22	3768.0
23	3939.5
24	4111.0
25	4887.5
26	6572.0
27	7480.0
28	8778.5
29	10077.0
30	11581.0
31	13085.0
32	15026.5
33	16968.0
34	18855.5
35	20743.0
36	22174.0
37	23605.0
38	25250.5
39	26896.0
40	28798.5
41	30701.0
42	32898.5
43	35096.0
44	38204.5
45	41313.0
46	41624.5
47	41936.0
48	42026.0
49	42116.0
50	41003.5
51	39891.0
52	38871.0
53	37851.0
54	36943.0
55	36035.0
56	35214.5
57	34394.0
58	33508.5
59	32623.0
60	30763.0
61	28903.0
62	26154.5
63	23406.0
64	20337.0
65	17268.0
66	14819.5
67	12371.0
68	10395.0
69	8419.0
70	6927.5
71	5436.0
72	4508.5
73	3581.0
74	2889.0
75	1733.5
76	1270.0
77	958.5
78	647.0
79	465.0
80	283.0
81	214.0
82	145.0
83	109.5
84	74.0
85	58.5
86	43.0
87	36.5
88	30.0
89	27.0
90	24.0
91	18.5
92	13.0
93	10.5
94	8.0
95	5.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	674150.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.41565261833611
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.81440577544056	32.62879959385512
2	10.384482175163905	9.847739988777503
3	5.988792941962835	8.518875772177587
4	4.192173538978694	7.950985769599779
5	3.0808134774972915	7.303939081544981
6	2.2726992073212116	6.465690967218625
7	1.6021469262545063	5.3176789469173045
8	1.1314321703875156	4.291807580184362
9	0.7860562724193559	3.3544234036350318
>10	1.7149312645121866	10.481751990770308
>50	0.021150082850116256	0.7011826717410576
>100	0.009892776535803238	0.8309052429953201
>500	3.411302253724148E-4	0.1297227963562625
>1k	3.411302253724148E-4	0.7992024149579712
>5k	3.411302253724148E-4	1.3772937792687967
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8515	1.2630720166135134	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4941	0.7329229399985167	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	802	0.1189646221167396	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013943484387747535	0.0
2	0.0	0.0	0.0	0.06022398575984573	0.0
3	0.0	0.0	0.0	0.09448935696803382	0.0
4	0.0	0.0	0.0	0.15352666320551805	0.0
5	0.0	0.0	0.0	0.34606541570867017	0.0
6	0.0	0.0	0.0	0.5872580286286435	0.0
7	0.0	0.0	0.0	0.7746050582214641	0.0
8	0.0	0.0	0.0	1.1773344211228955	0.0
9	0.0	0.0	0.0	1.4216420677890678	0.0
10	0.0	0.0	0.0	1.7806126233034192	0.0
11	0.0	0.0	0.0	2.0606689905807314	0.0
12	0.0	0.0	0.0	2.29429652154565	0.0
13	0.0	0.0	0.0	2.4162278424682935	0.0
14	0.0	0.0	0.0	2.5101238596751463	0.0
15	0.0	0.0	0.0	2.59630645998665	0.0
16	0.0	0.0	0.0	2.706370985685678	0.0
17	0.0	0.0	0.0	2.8297856560112735	0.0
18	0.0	0.0	0.0	2.9661054661425497	0.0
19	1.4833494029518654E-4	0.0	0.0	3.0662315508418008	0.0
20	1.4833494029518654E-4	0.0	0.0	3.1853445078988356	0.0
21	1.4833494029518654E-4	0.0	0.0	3.321664318030112	0.0
22	1.4833494029518654E-4	0.0	0.0	3.480679374026552	0.0
23	1.4833494029518654E-4	0.0	0.0	3.63821108062004	0.0
24	1.4833494029518654E-4	0.0	0.0	3.775124230512497	0.0
25	1.4833494029518654E-4	0.0	0.0	3.913817399688497	0.0
26	1.4833494029518654E-4	0.0	0.0	4.042423792924423	0.0
27	1.4833494029518654E-4	0.0	0.0	4.190313728398724	0.0
28	1.4833494029518654E-4	0.0	0.0	4.33434695542535	0.0
29	1.4833494029518654E-4	0.0	0.0	4.481940221019061	0.0
30	1.4833494029518654E-4	0.0	0.0	4.694355855521768	0.0
31	1.4833494029518654E-4	0.0	0.0	4.8673143959059555	0.0
32	1.4833494029518654E-4	0.0	0.0	5.0651932062597345	0.0
33	1.4833494029518654E-4	0.0	0.0	5.23696506712156	0.0
34	1.4833494029518654E-4	0.0	0.0	5.414818660535489	0.0
35	1.4833494029518654E-4	0.0	0.0	5.616257509456353	0.0
36	1.4833494029518654E-4	0.0	0.0	5.791292739004673	0.0
37	1.4833494029518654E-4	0.0	0.0	5.982051472224282	0.0
38	1.4833494029518654E-4	0.0	0.0	6.2165690128309725	0.0
39	1.4833494029518654E-4	0.0	0.0	6.554179336942817	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCTAC	20	7.031208E-4	45.000004	38
CGTTTTA	1930	0.0	44.067356	1
CGTTATT	770	0.0	42.954548	1
CGTTTTT	3240	0.0	42.430553	1
CACGACG	45	1.9263098E-8	40.0	26
CGGTCTA	45	1.9263098E-8	40.0	31
AGGCGAT	345	0.0	39.782608	7
GTTTTAT	2230	0.0	39.450672	2
AACACGT	155	0.0	39.193546	41
AAACACG	175	0.0	38.571426	40
AGCGTAA	30	1.13942115E-4	37.500004	1
ACTCGTT	30	1.13942115E-4	37.500004	45
GCGTATG	30	1.13942115E-4	37.500004	1
TAATCGT	60	1.546141E-10	37.500004	21
CACGACC	180	0.0	37.5	27
TCAAGCG	180	0.0	36.25	17
TCACGAC	50	4.8738002E-8	36.000004	25
TACGGGT	50	4.8738002E-8	36.000004	4
GTTACGA	25	0.0021064915	36.000004	1
ACACGAC	190	0.0	35.526314	26
>>END_MODULE