##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934699.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	254293
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.661846767311722	31.0	31.0	34.0	30.0	34.0
2	31.851498075055154	31.0	31.0	34.0	30.0	34.0
3	31.619777186159272	31.0	31.0	34.0	30.0	34.0
4	35.48490520777214	37.0	35.0	37.0	33.0	37.0
5	31.872281187449122	37.0	35.0	37.0	0.0	37.0
6	33.781999504508576	37.0	35.0	37.0	19.0	37.0
7	20.905777980518536	32.0	0.0	37.0	0.0	37.0
8	28.163724522499635	35.0	17.0	37.0	17.0	37.0
9	34.751546444455805	37.0	32.0	39.0	30.0	39.0
10	35.99646864050524	37.0	35.0	39.0	32.0	39.0
11	36.44494736386766	37.0	35.0	39.0	32.0	39.0
12	36.86664988812118	39.0	35.0	39.0	33.0	39.0
13	36.62151533860547	39.0	35.0	39.0	32.0	39.0
14	37.927339722288856	40.0	37.0	41.0	33.0	41.0
15	38.12313748313953	40.0	37.0	41.0	33.0	41.0
16	38.1974808586945	40.0	37.0	41.0	34.0	41.0
17	38.1838351822504	40.0	37.0	41.0	34.0	41.0
18	38.07208220438628	40.0	37.0	41.0	33.0	41.0
19	38.00770371185994	40.0	37.0	41.0	34.0	41.0
20	37.963243187976076	40.0	36.0	41.0	34.0	41.0
21	37.85834450810679	39.0	36.0	41.0	33.0	41.0
22	37.84247698520998	39.0	36.0	41.0	33.0	41.0
23	37.77536149245162	39.0	36.0	41.0	33.0	41.0
24	37.642616981198856	39.0	36.0	41.0	33.0	41.0
25	37.52048227831674	39.0	35.0	41.0	33.0	41.0
26	37.38127278375732	39.0	35.0	41.0	33.0	41.0
27	37.35644709056089	39.0	35.0	41.0	33.0	41.0
28	37.29385394013992	39.0	35.0	41.0	33.0	41.0
29	37.29190343422744	39.0	35.0	41.0	33.0	41.0
30	37.142162780729315	39.0	35.0	40.0	32.0	41.0
31	36.959747220725696	39.0	35.0	40.0	31.0	41.0
32	36.850766635337976	39.0	35.0	40.0	31.0	41.0
33	36.69006618349699	39.0	35.0	40.0	31.0	41.0
34	36.475471208409196	39.0	35.0	40.0	30.0	41.0
35	36.31345337858297	39.0	35.0	40.0	30.0	41.0
36	36.09082043154943	39.0	35.0	40.0	29.0	41.0
37	35.93984498196962	39.0	35.0	40.0	29.0	41.0
38	35.84580778865325	39.0	35.0	40.0	28.0	41.0
39	35.76955323190178	39.0	35.0	40.0	27.0	41.0
40	35.6525543369263	39.0	35.0	40.0	26.0	41.0
41	35.57230438903155	39.0	35.0	40.0	26.0	41.0
42	35.43958740507997	38.0	35.0	40.0	25.0	41.0
43	35.27288600158085	38.0	35.0	40.0	24.0	41.0
44	35.070060913985046	38.0	34.0	40.0	24.0	41.0
45	34.92820486604035	38.0	34.0	40.0	23.0	41.0
46	34.691627374721286	38.0	34.0	40.0	23.0	41.0
47	34.62028447499538	38.0	34.0	40.0	23.0	41.0
48	34.505570346018175	38.0	34.0	40.0	22.0	41.0
49	34.354185919392194	38.0	33.0	40.0	22.0	41.0
50	34.10975921476407	38.0	33.0	40.0	20.0	41.0
51	32.055872556460464	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	8.0
11	4.0
12	5.0
13	5.0
14	2.0
15	11.0
16	13.0
17	22.0
18	60.0
19	150.0
20	286.0
21	464.0
22	843.0
23	1372.0
24	2144.0
25	3009.0
26	3653.0
27	4283.0
28	4347.0
29	5167.0
30	6118.0
31	7487.0
32	9961.0
33	13724.0
34	18722.0
35	24515.0
36	29353.0
37	45437.0
38	44874.0
39	28244.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.76591176320229	19.45393699394006	23.407250691131885	18.372900551725767
2	31.33550667930301	24.680191747315106	25.738813101422377	18.24548847195951
3	30.008690762230973	23.3899478161019	30.516766092656894	16.084595329010238
4	25.923639266515398	25.720723732072848	29.153771436885794	19.20186556452596
5	20.89440134018632	36.30536428450645	26.005827922907827	16.794406452399397
6	23.807969546939947	34.86254045530156	28.128182844199408	13.201307153559084
7	47.6458258780226	42.216655590205	7.305745734251435	2.8317727975209697
8	80.49336788665043	4.282068322761539	10.962551072974875	4.262012717613147
9	75.22936140593724	6.77997428163575	13.048727255567396	4.941937056859607
10	35.782345562009176	29.783753386841163	20.761877047343024	13.672024003806632
11	26.804906151565323	25.534324578340733	29.868694773352	17.792074496741947
12	25.295230305199123	23.769431325282252	32.69220938051775	18.243128989000876
13	20.842099467936592	28.281155989350864	33.55342066041928	17.32332388229326
14	17.467252342769953	29.996106853118253	32.942314574132986	19.594326229978805
15	16.754295242102614	26.832040205589614	38.21811846963935	18.195546082668418
16	19.009960950557033	26.765581435588082	35.09141030228909	19.133047311565793
17	19.630898215837636	25.879988831780665	31.12590594314433	23.363207009237374
18	20.447672566684886	25.39354209514222	34.352105641917	19.806679696255895
19	20.682441121069004	27.566232652884665	31.371292170842295	20.380034055204035
20	21.899541080564546	26.891027279555473	32.6473792043037	18.562052435576284
21	19.995045085786867	28.422331719709153	33.10865812271671	18.47396507178727
22	19.77836590075228	26.014479360422822	31.35320280149277	22.85395193733213
23	17.583260254902807	28.154137156744387	32.035486623697864	22.227115964654946
24	18.009146928936307	25.697128902486497	35.417805444900175	20.875918723677017
25	17.044118398854867	28.035769761652894	32.866417872296914	22.053693967195322
26	17.715784547746104	29.84077422500816	31.51640037279831	20.92704085444743
27	19.194000621330513	28.681874845158934	32.092507461864855	20.031617071645698
28	16.461719355231956	27.963805531414547	34.71192679310874	20.862548320244755
29	17.706739863071338	25.980660104682396	33.56836405249063	22.744235979755636
30	19.585674792463813	27.589040988151464	32.06851938511874	20.756764834265983
31	20.14880472525787	28.213517477870013	30.62294282579544	21.01473497107667
32	19.858195074186078	27.86077477555418	30.310704580936164	21.970325569323577
33	19.998977557384592	26.57564305741802	28.992146854219346	24.433232530978046
34	16.449528693279014	27.0487193906242	32.18570703873092	24.316044877365872
35	17.54708151620375	26.87411765168526	30.01183673950915	25.56696409260184
36	21.109507536581816	27.06838174861282	29.484885545414148	22.337225169391214
37	17.80229892289603	28.19424836704117	31.39606674190796	22.607385968154844
38	16.267062011144624	28.315368492251064	29.714935133880992	25.702634362723316
39	18.139311738820965	27.563086675606485	29.593028514351555	24.70457307122099
40	19.936058011821007	26.628338176827516	29.4243254828092	24.01127832854227
41	17.159733063827947	26.341267750193676	29.062538095818603	27.436461090159774
42	19.057937104049266	25.629883638165424	30.060599387320924	25.251579870464386
43	19.689885289803495	25.656624445029948	30.766871286272135	23.886618978894425
44	19.21169674352027	26.769120660026037	29.581231099558387	24.437951496895312
45	19.247088987899787	27.126975575418903	28.384973239530776	25.24096219715053
46	20.04459422791819	27.673195880342753	28.267392338758835	24.014817552980226
47	17.991057559586775	27.117144396424596	30.787713385740073	24.104084658248556
48	18.394135898353476	25.72583594514989	30.80226353065165	25.07776462584499
49	19.400455380211014	24.216160098783686	31.421627807293163	24.961756713712134
50	18.37840601196258	25.810777331660724	30.648503891180646	25.162312765196056
51	17.602136118571885	25.5091567601153	28.064870051476053	28.82383706983676
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	235.0
1	457.0
2	679.0
3	2503.0
4	4327.0
5	2997.0
6	1667.0
7	1483.5
8	1300.0
9	1304.0
10	1308.0
11	1331.0
12	1354.0
13	1325.0
14	1296.0
15	1304.0
16	1312.0
17	1263.0
18	1214.0
19	1220.0
20	1226.0
21	1273.5
22	1321.0
23	1403.5
24	1486.0
25	1760.5
26	2447.0
27	2859.0
28	3455.5
29	4052.0
30	4650.5
31	5249.0
32	6153.0
33	7057.0
34	7892.0
35	8727.0
36	9259.0
37	9791.0
38	10949.5
39	12108.0
40	13593.5
41	15079.0
42	16170.5
43	17262.0
44	18054.5
45	18847.0
46	20187.0
47	21527.0
48	23236.5
49	24946.0
50	25080.0
51	25214.0
52	23226.0
53	21238.0
54	17730.5
55	14223.0
56	11635.5
57	9048.0
58	7423.0
59	5798.0
60	4841.0
61	3884.0
62	3256.5
63	2629.0
64	2150.0
65	1671.0
66	1295.5
67	920.0
68	726.0
69	532.0
70	457.0
71	382.0
72	312.5
73	243.0
74	188.5
75	95.0
76	56.0
77	43.0
78	30.0
79	22.5
80	15.0
81	9.0
82	3.0
83	3.0
84	3.0
85	2.5
86	2.0
87	3.0
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	254293.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.81256608278182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.11417008935946	40.725972037631735
2	8.812945077403528	9.30868488568601
3	3.809916176733679	6.0363434956082065
4	2.3256296402849994	4.9128987624651055
5	1.6589557992343449	4.380685638773898
6	1.3151522882631834	4.167394027969266
7	1.0557961692664306	3.903151347153188
8	0.8255893730053038	3.4881194655268013
9	0.635259710844628	3.0194725912839706
>10	2.424861606318317	17.767813144579065
>50	0.011985693399255025	0.43597546100945117
>100	0.00824016421198783	0.8715553000034673
>500	7.491058374534391E-4	0.33825682319698797
>1k	7.491058374534391E-4	0.6436770191128648
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1627	0.6398131289496761	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	855	0.33622632160539223	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	330	0.1297715627248882	No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	281	0.11050245189604117	RNA PCR Primer, Index 16 (96% over 25bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	258	0.10145776722127625	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1407824831985151	0.0
2	0.0	0.0	0.0	0.6303751971151388	0.0
3	0.0	0.0	0.0	0.8159878565277062	0.0
4	0.0	0.0	0.0	1.0448577035152364	0.0
5	0.0	0.0	0.0	1.6992209773764908	0.0
6	0.0	0.0	0.0	2.2328573731876222	0.0
7	0.0	0.0	0.0	2.774751959353974	0.0
8	0.0	0.0	0.0	4.100388134946695	0.0
9	0.0	0.0	0.0	4.548689897087218	0.0
10	0.0	0.0	0.0	5.7068027826169025	0.0
11	0.0	0.0	0.0	7.323441856441192	0.0
12	0.0	0.0	0.0	8.56452989268285	0.0
13	0.0	0.0	0.0	9.05333611227993	0.0
14	0.0	0.0	0.0	9.25428541092362	0.0
15	0.0	0.0	0.0	9.630622942825795	0.0
16	0.0	0.0	0.0	10.44818378799259	0.0
17	0.0	0.0	0.0	11.454503269850134	0.0
18	0.0	0.0	0.0	12.55677505869214	0.0
19	0.0	0.0	0.0	13.18282453704978	0.0
20	0.0	0.0	0.0	13.773481771027908	0.0
21	0.0	0.0	0.0	14.5540773831761	0.0
22	0.0	0.0	0.0	15.393266822130377	0.0
23	0.0	0.0	0.0	16.260770056588267	0.0
24	0.0	0.0	0.0	16.945413361751996	0.0
25	0.0	0.0	0.0	17.538430078688755	0.0
26	0.0	0.0	0.0	18.096447798405777	0.0
27	0.0	0.0	0.0	18.64699382208712	0.0
28	0.0	0.0	0.0	19.228999618550255	0.0
29	0.0	0.0	0.0	19.815331133770886	0.0
30	0.0	0.0	0.0	20.50744613497029	0.0
31	0.0	0.0	0.0	21.142147050842926	0.0
32	0.0	0.0	0.0	21.6993782762404	0.0
33	0.0	0.0	0.0	22.237733637968798	0.0
34	0.0	0.0	0.0	22.783167448573103	0.0
35	0.0	0.0	0.0	23.397812759297345	0.0
36	0.0	0.0	0.0	23.956223726174137	0.0
37	0.0	0.0	0.0	24.48986012198527	0.0
38	0.0	0.0	0.0	25.03018171951253	0.0
39	0.0	0.0	0.0	25.575615530116835	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAG	35	1.2069358E-7	45.000004	2
ACGGGAC	35	1.2069358E-7	45.000004	5
TAAGGCG	30	2.1588112E-6	45.000004	5
TCACGGC	20	7.0229155E-4	45.0	35
CGGGTCA	25	3.88221E-5	45.0	6
GTCGAGG	25	3.88221E-5	45.0	3
ATAGGCG	20	7.0229155E-4	45.0	5
TTTACGG	20	7.0229155E-4	45.0	2
ACGCACG	20	7.0229155E-4	45.0	1
CGCGCAA	20	7.0229155E-4	45.0	18
TCGGCGG	20	7.0229155E-4	45.0	15
ATCGTCC	20	7.0229155E-4	45.0	39
AACGGGT	20	7.0229155E-4	45.0	4
CCATCGT	20	7.0229155E-4	45.0	37
CTACGAA	40	6.7793735E-9	45.0	11
TACGAAT	40	6.7793735E-9	45.0	12
CAAGGCG	25	3.88221E-5	45.0	5
CGGGCTA	20	7.0229155E-4	45.0	6
AACGCGG	20	7.0229155E-4	45.0	2
TCGTCCT	20	7.0229155E-4	45.0	40
>>END_MODULE