FastQCFastQC Report
Sat 14 Jan 2017
SRR2934697.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934697.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499286
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49120.9838048733591568No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28750.5758222742075684No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC253.8876256E-545.025
TATGACG253.8876256E-545.01
TATTACG207.0294493E-445.01
TATACGG253.8876256E-545.02
CGTTTTA10600.043.7264141
AAGGCGA850.042.3529436
CGTTTTT15650.041.549521
GCGATGT650.041.5384649
AGGCGAT2450.041.326537
ACAACGA1100.040.90909213
CGTTATT4500.040.51
GTTTTAT12500.039.2399982
CACAACG1150.039.13043212
ACACGAC750.039.00000426
GCGCGAA356.241191E-638.5714261
TACGGTT356.241191E-638.57142633
CCGTAGG356.241191E-638.5714263
GTACGGG2000.038.253
TACGAAA1650.038.18181620
CATACGA1650.038.18181618
Produced by FastQC (version 0.11.5)