##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934697.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	499286
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.871983191998172	31.0	31.0	34.0	30.0	34.0
2	32.06192042236314	33.0	31.0	34.0	30.0	34.0
3	31.722221332062183	33.0	31.0	34.0	30.0	34.0
4	35.62332410682455	37.0	35.0	37.0	33.0	37.0
5	32.01787953197166	37.0	35.0	37.0	0.0	37.0
6	33.89734140352423	37.0	35.0	37.0	19.0	37.0
7	20.874336552597107	32.0	0.0	37.0	0.0	37.0
8	28.145551848039	35.0	17.0	37.0	17.0	37.0
9	34.846827269340615	37.0	32.0	39.0	32.0	39.0
10	36.25136494914738	37.0	35.0	39.0	32.0	39.0
11	36.62739792423581	38.0	35.0	39.0	33.0	39.0
12	36.94145840259891	39.0	37.0	39.0	33.0	39.0
13	36.70845767756356	39.0	35.0	39.0	33.0	39.0
14	38.014480678408766	40.0	37.0	41.0	33.0	41.0
15	38.172700616480334	40.0	37.0	41.0	33.0	41.0
16	38.23820215267402	40.0	37.0	41.0	33.0	41.0
17	38.18981105017966	40.0	37.0	41.0	33.0	41.0
18	38.051349326838725	40.0	37.0	41.0	33.0	41.0
19	37.95454909610925	40.0	37.0	41.0	33.0	41.0
20	37.877777466221765	40.0	36.0	41.0	33.0	41.0
21	37.79059496961661	40.0	36.0	41.0	33.0	41.0
22	37.716847658456274	39.0	36.0	41.0	33.0	41.0
23	37.65645541833739	39.0	35.0	41.0	33.0	41.0
24	37.52862687918348	39.0	35.0	41.0	33.0	41.0
25	37.37875486194285	39.0	35.0	41.0	33.0	41.0
26	37.23255408723657	39.0	35.0	41.0	33.0	41.0
27	37.19782249051646	39.0	35.0	41.0	33.0	41.0
28	37.109093785926305	39.0	35.0	41.0	32.0	41.0
29	37.05321799529728	39.0	35.0	41.0	32.0	41.0
30	36.85720809315703	39.0	35.0	41.0	31.0	41.0
31	36.6979566821421	39.0	35.0	41.0	31.0	41.0
32	36.538569076641444	39.0	35.0	40.0	31.0	41.0
33	36.332352599512106	39.0	35.0	40.0	30.0	41.0
34	36.06527521300417	39.0	35.0	40.0	29.0	41.0
35	35.77967737929764	39.0	35.0	40.0	27.0	41.0
36	35.59672612490637	38.0	35.0	40.0	26.0	41.0
37	35.493430618923824	38.0	35.0	40.0	26.0	41.0
38	35.3490103868324	38.0	34.0	40.0	25.0	41.0
39	35.23525594549016	38.0	34.0	40.0	25.0	41.0
40	35.126412517074385	38.0	34.0	40.0	24.0	41.0
41	34.980369968314754	38.0	34.0	40.0	24.0	41.0
42	34.85483069823708	38.0	34.0	40.0	23.0	41.0
43	34.76433947677283	38.0	34.0	40.0	23.0	41.0
44	34.60865315670778	38.0	34.0	40.0	23.0	41.0
45	34.50927123932976	38.0	34.0	40.0	23.0	41.0
46	34.4011608577048	38.0	33.0	40.0	23.0	41.0
47	34.34456804316564	37.0	33.0	40.0	23.0	41.0
48	34.2273025880958	37.0	33.0	40.0	22.0	41.0
49	34.1128972172262	37.0	33.0	40.0	22.0	41.0
50	33.9420933092456	37.0	33.0	40.0	20.0	41.0
51	32.12595786783527	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	27.0
10	20.0
11	20.0
12	16.0
13	11.0
14	21.0
15	35.0
16	77.0
17	125.0
18	229.0
19	410.0
20	801.0
21	1210.0
22	1970.0
23	3075.0
24	4866.0
25	6731.0
26	8394.0
27	9018.0
28	9524.0
29	10181.0
30	11774.0
31	14917.0
32	19410.0
33	28201.0
34	37473.0
35	45805.0
36	56119.0
37	88833.0
38	86007.0
39	53969.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.09508377963732	17.51961801452474	20.064452037509565	21.320846168328373
2	35.30501556222285	22.457469266112007	24.784592397944262	17.452922773720875
3	31.41405927664705	22.82219008744487	30.026277524304707	15.73747311160337
4	27.557351898511072	24.723905737392997	29.430026077238296	18.288716286857632
5	21.53935820351462	36.145015081536435	26.065621707798737	16.25000500715021
6	25.10945630360154	33.30576062617418	28.439812051609696	13.144971018614582
7	47.5044363350865	42.38893139403068	7.677964132781612	2.4286681381012083
8	82.11806459624343	3.9430306477650086	11.28150999627468	2.6573947597168757
9	76.45257427606622	6.158394186898892	13.044227156379309	4.344804380655576
10	42.77948911044972	24.393233537491536	19.954695304895388	12.87258204716335
11	32.04776420728801	24.23440673281446	26.957895875309944	16.75993318458759
12	29.176664276586965	21.753263660507205	30.492343065898105	18.57772899700773
13	25.50742460233213	21.913492467243223	32.35780694832221	20.221275982102444
14	22.099558169065425	22.44705439367417	34.137748705150955	21.315638732109452
15	22.285824156896048	23.21495094995654	35.07368522249772	19.425539670649687
16	26.99815336300237	22.481703873130833	31.934202040513853	18.585940723352948
17	26.116894925954266	24.18513637474313	30.221356096505808	19.476612602796795
18	26.396894765725452	22.947368842707387	32.03314332867335	18.622593062893813
19	25.182760982683273	25.901387180894318	29.72524765364941	19.190604182773
20	26.35303212988147	25.93243151219942	30.02727895434681	17.6872574035723
21	27.19984137348133	25.34879808366347	30.115004226034774	17.33635631682042
22	25.034949908469294	22.84041611421109	32.697892590619404	19.426741386700208
23	24.598927268138905	23.685422783735174	32.03935219493437	19.67629775319156
24	24.618955868980905	22.61729750083119	32.43651934963127	20.327227280556635
25	23.90493624896352	24.387024671230517	30.346735137776744	21.361303942029217
26	22.426625220815325	24.987081552456907	30.80258609294072	21.783707133787047
27	21.44782749766667	24.158097763606428	32.021126168168145	22.372948570558755
28	20.227284562355045	26.305964917902763	31.185332655031385	22.281417864710807
29	22.778928309626146	25.364220106311812	30.565848030988253	21.29100355307379
30	23.526796265066515	24.07638107217106	31.448708756103716	20.94811390665871
31	24.468340790649044	24.00347696510617	30.795375796637597	20.73280644760718
32	24.403247837912538	24.702475134492055	29.695204752386406	21.199072275208998
33	23.7467103023117	24.897954278709996	30.229567822851035	21.12576759612727
34	21.599243720032206	25.026137324098812	32.35880837836431	21.015810577504677
35	21.583220839358606	26.265507144201923	31.770768657643117	20.38050335879636
36	22.427626650857423	29.02264433611197	27.904046979086132	20.645682033944475
37	22.757697992733625	28.63449005179396	28.159611925830085	20.44820002964233
38	22.785738033912427	27.826336007819165	28.53995505581971	20.847970902448697
39	22.80336320265339	27.422759700852815	27.87120007370525	21.90267702278854
40	24.313319420131947	25.11826888797202	28.26856751441058	22.29984417748545
41	21.148199629070312	25.81326133718951	28.88665013639477	24.15188889734541
42	21.096926410914786	26.297552905549125	28.86061295530017	23.74490772823592
43	21.573406824946023	25.278697980716462	29.611084628849998	23.536810565487514
44	21.72642533537892	25.01572245166097	29.2780089968475	23.97984321611261
45	21.870230689424496	25.459556246319742	29.227336636717233	23.442876427538526
46	22.06611040565928	26.228654518652633	28.672143821376928	23.033091254311156
47	20.692348673906338	25.72934149966152	30.752514590835712	22.825795235596434
48	21.305824717696872	25.410085602240002	31.03652015077531	22.247569529287823
49	22.913520507284403	25.08762512868376	29.80956806319424	22.189286300837598
50	21.417384024386823	25.2690842523123	30.490540491822323	22.82299123147855
51	21.168428515920734	25.00570815123997	30.12161366431264	23.704249668526657
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	256.0
1	718.5
2	1181.0
3	7405.0
4	13629.0
5	8919.0
6	4209.0
7	3640.5
8	3072.0
9	2903.0
10	2734.0
11	2702.5
12	2671.0
13	2665.0
14	2659.0
15	2515.0
16	2371.0
17	2334.5
18	2298.0
19	2279.5
20	2261.0
21	2325.5
22	2390.0
23	2653.5
24	2917.0
25	3374.0
26	4391.0
27	4951.0
28	5908.0
29	6865.0
30	7971.0
31	9077.0
32	10251.0
33	11425.0
34	12588.5
35	13752.0
36	14899.0
37	16046.0
38	17138.5
39	18231.0
40	19725.0
41	21219.0
42	22486.0
43	23753.0
44	25047.5
45	26342.0
46	28470.0
47	30598.0
48	32034.5
49	33471.0
50	33461.5
51	33452.0
52	32506.5
53	31561.0
54	30876.5
55	30192.0
56	29583.0
57	28974.0
58	28216.5
59	27459.0
60	25643.0
61	23827.0
62	21750.5
63	19674.0
64	17101.0
65	14528.0
66	12181.0
67	9834.0
68	8209.0
69	6584.0
70	5446.0
71	4308.0
72	3533.0
73	2758.0
74	2286.5
75	1385.0
76	955.0
77	756.0
78	557.0
79	418.0
80	279.0
81	219.5
82	160.0
83	113.0
84	66.0
85	52.5
86	39.0
87	28.5
88	18.0
89	18.5
90	19.0
91	12.0
92	5.0
93	7.0
94	9.0
95	6.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	499286.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.98906041407189
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.60556258594242	36.51100356678224
2	10.47189451158004	10.67904123801485
3	5.5313778090119685	8.461192718303037
4	3.561602776566274	7.264111165810558
5	2.4237857319325906	6.179327855813815
6	1.77709019362964	5.436729554654185
7	1.3235671145532204	4.724121049622307
8	0.9436900060590886	3.8494293384882194
9	0.6902704849904301	3.167661911510497
>10	1.6525560166735547	11.041664812563106
>50	0.012401846041488276	0.4187116487160393
>100	0.005315076873698004	0.5083710469727268
>500	0.0	0.0
>1k	8.858461456163341E-4	1.758634092748389
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4912	0.9838048733591568	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2875	0.5758222742075684	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016623738698861974	0.0
2	0.0	0.0	0.0	0.06809724286280809	0.0
3	0.0	0.0	0.0	0.10274672231947221	0.0
4	0.0	0.0	0.0	0.13899848984349653	0.0
5	0.0	0.0	0.0	0.2595706669123508	0.0
6	0.0	0.0	0.0	0.496509014873239	0.0
7	0.0	0.0	0.0	0.7036047475795436	0.0
8	0.0	0.0	0.0	1.1588548447182576	0.0
9	0.0	0.0	0.0	1.4584827133146132	0.0
10	0.0	0.0	0.0	1.8668658844830417	0.0
11	0.0	0.0	0.0	2.2261789835885644	0.0
12	0.0	0.0	0.0	2.500370529115577	0.0
13	0.0	0.0	0.0	2.650585035430595	0.0
14	0.0	0.0	0.0	2.761142912078448	0.0
15	0.0	0.0	0.0	2.8652916364568606	0.0
16	0.0	0.0	0.0	3.011500422603478	0.0
17	0.0	0.0	0.0	3.173732089423697	0.0
18	0.0	0.0	0.0	3.352787780951198	0.0
19	0.0	0.0	0.0	3.487179692601034	0.0
20	0.0	0.0	0.0	3.645205353244433	0.0
21	0.0	0.0	0.0	3.8232596147298343	0.0
22	0.0	0.0	0.0	4.010126460585717	0.0
23	0.0	0.0	0.0	4.20921075295522	0.0
24	0.0	0.0	0.0	4.368638415657559	0.0
25	0.0	0.0	0.0	4.54569124710086	0.0
26	0.0	0.0	0.0	4.720941504468381	0.0
27	0.0	0.0	0.0	4.918824080787364	0.0
28	0.0	0.0	0.0	5.103287494542206	0.0
29	0.0	0.0	0.0	5.290955484431769	0.0
30	0.0	0.0	0.0	5.578766478531342	0.0
31	0.0	0.0	0.0	5.786463069262908	0.0
32	0.0	0.0	0.0	5.9901539398260715	0.0
33	0.0	0.0	0.0	6.1850322260187545	0.0
34	0.0	0.0	0.0	6.39413081880926	0.0
35	0.0	0.0	0.0	6.620654294332306	0.0
36	2.002860084200238E-4	0.0	0.0	6.861598362461595	0.0
37	2.002860084200238E-4	0.0	0.0	7.095732706304603	0.0
38	2.002860084200238E-4	0.0	0.0	7.409180309481941	0.0
39	2.002860084200238E-4	0.0	0.0	7.850009814014412	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	25	3.8876256E-5	45.0	25
TATGACG	25	3.8876256E-5	45.0	1
TATTACG	20	7.0294493E-4	45.0	1
TATACGG	25	3.8876256E-5	45.0	2
CGTTTTA	1060	0.0	43.726414	1
AAGGCGA	85	0.0	42.352943	6
CGTTTTT	1565	0.0	41.54952	1
GCGATGT	65	0.0	41.538464	9
AGGCGAT	245	0.0	41.32653	7
ACAACGA	110	0.0	40.909092	13
CGTTATT	450	0.0	40.5	1
GTTTTAT	1250	0.0	39.239998	2
CACAACG	115	0.0	39.130432	12
ACACGAC	75	0.0	39.000004	26
GCGCGAA	35	6.241191E-6	38.571426	1
TACGGTT	35	6.241191E-6	38.571426	33
CCGTAGG	35	6.241191E-6	38.571426	3
GTACGGG	200	0.0	38.25	3
TACGAAA	165	0.0	38.181816	20
CATACGA	165	0.0	38.181816	18
>>END_MODULE