##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934696.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	407481
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98411214265205	33.0	31.0	34.0	30.0	34.0
2	32.17609410009301	33.0	31.0	34.0	30.0	34.0
3	32.039866889499145	33.0	31.0	34.0	30.0	34.0
4	35.8162540093894	37.0	35.0	37.0	35.0	37.0
5	32.07003762138603	37.0	35.0	37.0	0.0	37.0
6	33.95185296983172	37.0	35.0	37.0	19.0	37.0
7	20.74513412895325	32.0	0.0	37.0	0.0	37.0
8	28.095521018157903	35.0	17.0	37.0	17.0	37.0
9	34.86456791850418	37.0	32.0	39.0	32.0	39.0
10	36.30078212235662	37.0	35.0	39.0	32.0	39.0
11	36.6725761446546	38.0	35.0	39.0	33.0	39.0
12	36.98487536842209	39.0	37.0	39.0	33.0	39.0
13	36.733852621349214	39.0	35.0	39.0	33.0	39.0
14	38.02135068874377	40.0	37.0	41.0	33.0	41.0
15	38.20580836897917	40.0	37.0	41.0	33.0	41.0
16	38.30487801885241	40.0	37.0	41.0	34.0	41.0
17	38.28540962646111	40.0	37.0	41.0	34.0	41.0
18	38.21187736360714	40.0	37.0	41.0	34.0	41.0
19	38.16874897234472	40.0	37.0	41.0	34.0	41.0
20	38.14352080219691	40.0	37.0	41.0	34.0	41.0
21	38.02765773128072	40.0	37.0	41.0	33.0	41.0
22	38.021316331313606	40.0	37.0	41.0	34.0	41.0
23	37.970356900076325	40.0	36.0	41.0	34.0	41.0
24	37.83881211639316	40.0	36.0	41.0	33.0	41.0
25	37.68930330493937	40.0	36.0	41.0	33.0	41.0
26	37.55697811677109	39.0	36.0	41.0	33.0	41.0
27	37.50939798420049	39.0	35.0	41.0	33.0	41.0
28	37.43081517911265	39.0	35.0	41.0	33.0	41.0
29	37.36941354320815	39.0	35.0	41.0	33.0	41.0
30	37.162387939560375	39.0	35.0	41.0	32.0	41.0
31	37.016825324370956	39.0	35.0	41.0	31.0	41.0
32	36.89815476059007	39.0	35.0	41.0	31.0	41.0
33	36.71770462917289	39.0	35.0	41.0	31.0	41.0
34	36.54233448921545	39.0	35.0	41.0	30.0	41.0
35	36.33117863164172	39.0	35.0	41.0	30.0	41.0
36	36.12738262642921	39.0	35.0	40.0	29.0	41.0
37	36.06143108513035	39.0	35.0	40.0	29.0	41.0
38	35.89710685897011	39.0	35.0	40.0	28.0	41.0
39	35.77989403186897	39.0	35.0	40.0	27.0	41.0
40	35.64115136656678	39.0	35.0	40.0	27.0	41.0
41	35.46133930170977	38.0	35.0	40.0	26.0	41.0
42	35.35651723638648	38.0	34.0	40.0	26.0	41.0
43	35.285679577698104	38.0	34.0	40.0	25.0	41.0
44	35.094833869554655	38.0	34.0	40.0	25.0	41.0
45	34.94577170469298	38.0	34.0	40.0	24.0	41.0
46	34.78697166248242	38.0	34.0	40.0	23.0	41.0
47	34.68905298651962	38.0	34.0	40.0	23.0	41.0
48	34.541453466541995	37.0	33.0	40.0	23.0	41.0
49	34.401002746140314	37.0	33.0	40.0	23.0	41.0
50	34.22573813257551	37.0	33.0	40.0	23.0	41.0
51	32.26571054846729	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	15.0
9	10.0
10	20.0
11	19.0
12	10.0
13	9.0
14	20.0
15	26.0
16	39.0
17	61.0
18	147.0
19	262.0
20	491.0
21	844.0
22	1416.0
23	2058.0
24	3030.0
25	4149.0
26	5192.0
27	6025.0
28	6740.0
29	7775.0
30	9180.0
31	11759.0
32	15436.0
33	22347.0
34	30026.0
35	37699.0
36	47027.0
37	74903.0
38	74382.0
39	46355.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.66824710845414	17.969426795359784	22.807443782654897	16.554882313531184
2	31.62994102792523	24.018052375448182	26.494977679940906	17.857028916685685
3	31.94259364240296	23.58686662691021	27.551959477865225	16.918580252821606
4	28.28892635484845	25.82500779177434	26.450803841160692	19.43526201221652
5	22.542400749973616	37.28591026330062	23.20648079296949	16.965208193756272
6	26.523445264932597	34.00575732365436	25.8075836664777	13.663213744935346
7	49.78244384400745	42.80199567587201	4.958513403078917	2.457047077041629
8	86.03444086963565	3.753794655456328	7.330648545576358	2.8811159293316746
9	80.19686807483049	6.287409719716992	9.060790564468038	4.454931640984488
10	44.52035800442229	26.362456163600267	15.662325359955434	13.454860472022009
11	31.864307783675805	24.537585801546573	25.13025147184777	18.467854942929854
12	29.320385490366423	21.896481062920724	29.35376127966703	19.429372167045823
13	24.836986264390244	24.397211158311677	30.13220248306056	20.633600094237522
14	20.792871324061736	25.024725079206146	31.318024644093832	22.86437895263828
15	20.29125284369087	24.62765135061512	32.89085871488487	22.19023709080914
16	24.094865772882663	24.090202978789197	30.64241032097202	21.17252092735612
17	24.7802965046223	24.819562139093602	28.41384015451027	21.986301201773824
18	25.844395198794544	23.47986777297592	29.211177944493116	21.46455908373642
19	24.48212309285586	26.534734134843095	26.3438049872264	22.63933778507464
20	26.810329806788534	25.738377985722032	27.779209337367877	19.672082870121553
21	26.506021139635962	26.259874693543996	28.078609800211545	19.155494366608504
22	26.445650226636335	22.965242551186435	28.693362389902845	21.89574483227439
23	25.238477376859294	25.015399491019213	28.422674922266317	21.323448209855183
24	23.809699102534843	24.14713814877258	31.02745894900621	21.015703799686367
25	23.50048223107335	25.903048240286054	28.723057025971766	21.873412502668835
26	23.079849121799544	25.406583374439546	27.90657723918416	23.60699026457675
27	21.822121767640702	25.439222933093813	29.860778784777697	22.87787651448779
28	20.51531237039273	26.3970590039781	29.224675506342628	23.86295311928654
29	22.38951018575099	25.084114351343988	29.42762975451616	23.098745708388858
30	23.720860604543525	25.107919142242213	29.065404276518414	22.105815976695848
31	25.389895479789242	25.216390457469185	27.972592587139033	21.421121475602543
32	25.517999612251856	25.071843840571706	27.44716931586994	21.962987231306492
33	24.572434052139855	24.894166844589073	27.878355064407913	22.655044038863164
34	22.266805078028177	24.609736404887588	30.784012015284144	22.339446501800083
35	23.019232798584476	26.184533757402185	29.349589306004454	21.44664413800889
36	23.805527128872267	27.699451017348046	26.99070631514107	21.50431553863861
37	22.89309194784542	27.297714494663555	28.040325806602024	21.768867750888997
38	22.64105565658276	26.975000061352556	28.514212932627537	21.869731349437153
39	22.413314976649218	26.402212618502457	27.988053430712107	23.19641897413622
40	25.649048667299823	24.742257921228227	27.722764987815385	21.885928423656566
41	21.587264191459234	24.550347132749746	28.815822087410208	25.04656658838081
42	21.6145047253737	24.89833881825165	29.07914724858337	24.408009207791284
43	21.676348099665997	24.93588658121483	29.065649686733856	24.322115632385312
44	22.52154088166074	24.801647193366072	28.29015340592568	24.386658519047515
45	22.440555510563684	24.729742000240503	27.85479568372513	24.974906805470685
46	22.566696361302736	25.563891322540194	27.471219516983613	24.398192799173458
47	20.873365874727902	25.744513241108173	30.27748533060437	23.104635553559554
48	21.10699639983214	25.57370773115802	30.860089182072294	22.45920668693755
49	22.864869773069174	24.971225652239003	29.598680674681766	22.56522390001006
50	22.16839558163448	25.34915738402527	29.912805750452172	22.569641283888085
51	21.776966287998704	24.62814217104601	28.444271021225532	25.150620519729756
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	177.0
1	546.5
2	916.0
3	2779.5
4	4643.0
5	3058.5
6	1474.0
7	1320.0
8	1166.0
9	1159.0
10	1152.0
11	1168.5
12	1185.0
13	1231.5
14	1278.0
15	1228.5
16	1179.0
17	1190.5
18	1202.0
19	1234.5
20	1267.0
21	1446.5
22	1626.0
23	1840.5
24	2055.0
25	2516.5
26	3777.5
27	4577.0
28	5052.0
29	5527.0
30	6374.0
31	7221.0
32	8570.5
33	9920.0
34	11473.5
35	13027.0
36	13644.0
37	14261.0
38	15099.0
39	15937.0
40	17581.5
41	19226.0
42	20913.5
43	22601.0
44	23596.5
45	24592.0
46	25757.0
47	26922.0
48	27244.5
49	27567.0
50	26698.0
51	25829.0
52	25262.0
53	24695.0
54	24548.0
55	24401.0
56	24796.5
57	25192.0
58	24091.0
59	22990.0
60	21111.0
61	19232.0
62	17391.0
63	15550.0
64	13772.5
65	11995.0
66	10139.5
67	8284.0
68	7023.5
69	5763.0
70	4826.0
71	3889.0
72	3256.0
73	2623.0
74	2086.5
75	1233.0
76	916.0
77	695.0
78	474.0
79	341.5
80	209.0
81	152.5
82	96.0
83	71.5
84	47.0
85	38.5
86	30.0
87	25.5
88	21.0
89	15.0
90	9.0
91	6.5
92	4.0
93	3.5
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	407481.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.01770847980768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.3493087851013	32.37313983428765
2	9.372793337882902	8.626289429283581
3	5.005534053409357	6.910296205666257
4	3.6414773722760327	6.702897766128584
5	2.664751393104697	6.131287638952668
6	2.1090721629567595	5.823280077469296
7	1.6656093123829232	5.3653266644943205
8	1.3421373833054524	4.940966947584177
9	0.9842951892302174	4.076550816846596
>10	2.836958275446779	17.105800543846104
>50	0.019808989343711975	0.6084691681797764
>100	0.00715324615189553	0.6720254566579467
>500	5.502497039919638E-4	0.23700671719360894
>1k	5.502497039919638E-4	0.4266627334094349
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1685	0.41351621302588343	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	936	0.2297039616571079	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0331303790851598	0.0
2	0.0	0.0	0.0	0.135957259356878	0.0
3	0.0	0.0	0.0	0.18602094330778612	0.0
4	0.0	0.0	0.0	0.24393775415295438	0.0
5	0.0	0.0	0.0	0.41621572539578533	0.0
6	0.0	0.0	0.0	0.6189245633538741	0.0
7	0.0	0.0	0.0	0.7941474571820527	0.0
8	0.0	0.0	0.0	1.2233699239964562	0.0
9	0.0	0.0	0.0	1.4233792495846431	0.0
10	0.0	0.0	0.0	1.8253611824845821	0.0
11	0.0	0.0	0.0	2.3677177586194205	0.0
12	0.0	0.0	0.0	2.7726446141047068	0.0
13	0.0	0.0	0.0	2.954493583749917	0.0
14	0.0	0.0	0.0	3.0627194887614393	0.0
15	0.0	0.0	0.0	3.2023579013500014	0.0
16	0.0	0.0	0.0	3.4671555238158343	0.0
17	0.0	0.0	0.0	3.7866796243260423	0.0
18	0.0	0.0	0.0	4.117492594746749	0.0
19	0.0	0.0	0.0	4.354558862867226	0.0
20	0.0	0.0	0.0	4.585980696032453	0.0
21	0.0	0.0	0.0	4.883663287368	0.0
22	0.0	0.0	0.0	5.209568053479794	0.0
23	0.0	0.0	0.0	5.544552997563077	0.0
24	2.4541021544562813E-4	0.0	0.0	5.814995054984159	0.0
25	2.4541021544562813E-4	0.0	0.0	6.06752216667771	0.0
26	2.4541021544562813E-4	0.0	0.0	6.299680230489274	0.0
27	2.4541021544562813E-4	0.0	0.0	6.5819019782517465	0.0
28	4.908204308912563E-4	0.0	0.0	6.839337294254211	0.0
29	4.908204308912563E-4	0.0	0.0	7.104380326935489	0.0
30	4.908204308912563E-4	0.0	0.0	7.543419202367717	0.0
31	4.908204308912563E-4	0.0	0.0	7.8391385119797	0.0
32	4.908204308912563E-4	0.0	0.0	8.174368866278428	0.0
33	4.908204308912563E-4	0.0	0.0	8.47573261084566	0.0
34	4.908204308912563E-4	0.0	0.0	8.803355248465573	0.0
35	4.908204308912563E-4	0.0	0.0	9.151346933967472	0.0
36	4.908204308912563E-4	0.0	0.0	9.445593782286782	0.0
37	4.908204308912563E-4	0.0	0.0	9.765854113443327	0.0
38	4.908204308912563E-4	0.0	0.0	10.116054490884238	0.0
39	4.908204308912563E-4	0.0	0.0	10.651294170771152	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGAACT	20	7.027919E-4	45.000004	41
CGTTTAG	20	7.027919E-4	45.000004	2
GCGCGAA	25	3.886358E-5	45.0	1
TACCGGG	25	3.886358E-5	45.0	3
CGTTATT	155	0.0	42.09677	1
CGTTTTA	220	0.0	41.93182	1
GCGATGT	70	0.0	41.785717	9
AGGCGAT	220	0.0	40.909092	7
TACGGGA	100	0.0	40.5	4
AGGATCG	45	1.9230356E-8	40.0	7
ACACGCT	40	3.4508957E-7	39.375004	14
TCGATCA	40	3.4508957E-7	39.375004	17
ACTAGGC	40	3.4508957E-7	39.375004	4
TGACACG	40	3.4508957E-7	39.375004	12
CTCGATC	40	3.4508957E-7	39.375004	16
CTTACGG	40	3.4508957E-7	39.375004	2
ATAGGGT	75	0.0	39.0	5
AGGATCA	145	0.0	38.793106	7
CGCATAG	35	6.238364E-6	38.57143	2
CGTTAGG	35	6.238364E-6	38.57143	3
>>END_MODULE