FastQCFastQC Report
Sat 14 Jan 2017
SRR2934695.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934695.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535584
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23520.43914680050188204No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13910.25971649638525424No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT207.029907E-445.00000428
CGAATAA207.029907E-445.00000426
CGTTAAG302.1633223E-644.9999962
GTTAACG302.1633223E-644.9999961
CGTTTTA5150.042.8155331
TACGAAT900.042.512
CGAGACA1700.041.0294122
TTAACGG556.002665E-1140.9090922
CGTTATT2650.040.754721
TAGGCGA1050.040.7142876
CACAACG3100.040.64516412
ACAACGA3150.040.013
CGTCATA451.9250365E-840.038
CGACCAA1750.039.85714329
ACGACCA1750.039.85714328
AACACGT1650.039.54545641
CACGACC1800.038.7527
TCAAGCG1800.038.7517
CTCGTAC356.242042E-638.57142629
TACGGTT356.242042E-638.57142633
Produced by FastQC (version 0.11.5)