FastQCFastQC Report
Sat 14 Jan 2017
SRR2934692.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934692.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences313505
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19510.6223186233074433No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11280.35980287395735316No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.14672812235849508No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGGC253.8842936E-545.0000044
GCGATCT406.7884685E-945.0000049
TAGGCCG207.0254295E-445.0000046
AGTACGG207.0254295E-445.0000042
CGAAGGA453.8198777E-1045.0000044
CGAATAT302.1604337E-644.99999614
CGTTTAG302.1604337E-644.9999962
GACCGAT1450.043.4482739
CGTTATT1400.043.3928571
CGTTTTA4800.043.1249961
AGGCGAT1450.041.896557
GCCGATG451.920671E-840.0000049
CGTTTTT7700.039.448051
GTAGGAC403.4474942E-739.3750045
ACACGAC1900.039.0789526
TACGAAT356.233762E-638.57142612
TAAGGTC356.233762E-638.5714265
GCTACGA356.233762E-638.57142610
CACGACC2000.038.25000427
ACGACCA2000.038.25000428
Produced by FastQC (version 0.11.5)