##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934692.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	313505
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02269501283871	33.0	31.0	34.0	30.0	34.0
2	32.212529305752696	33.0	31.0	34.0	30.0	34.0
3	31.959834771375256	33.0	31.0	34.0	30.0	34.0
4	35.79238608634631	37.0	35.0	37.0	35.0	37.0
5	32.09056952839668	37.0	35.0	37.0	0.0	37.0
6	33.97758887418063	37.0	35.0	37.0	19.0	37.0
7	20.617792379706863	32.0	0.0	37.0	0.0	37.0
8	28.045026395113315	35.0	17.0	37.0	17.0	37.0
9	34.907931292961834	37.0	32.0	39.0	32.0	39.0
10	36.3086075182214	37.0	35.0	39.0	32.0	39.0
11	36.72144303918598	38.0	37.0	39.0	33.0	39.0
12	37.08097478509114	39.0	37.0	39.0	34.0	39.0
13	36.879057750275116	39.0	37.0	39.0	33.0	39.0
14	38.20156935296088	40.0	38.0	41.0	33.0	41.0
15	38.3680962026124	40.0	38.0	41.0	34.0	41.0
16	38.467160013396914	40.0	38.0	41.0	34.0	41.0
17	38.4399802236009	40.0	38.0	41.0	34.0	41.0
18	38.33152262324365	40.0	38.0	41.0	34.0	41.0
19	38.268805920160766	40.0	37.0	41.0	34.0	41.0
20	38.2169821852921	40.0	37.0	41.0	34.0	41.0
21	38.10397601314174	40.0	37.0	41.0	34.0	41.0
22	38.07876748377219	40.0	37.0	41.0	34.0	41.0
23	38.010940814341076	40.0	36.0	41.0	34.0	41.0
24	37.888792842219424	40.0	36.0	41.0	33.0	41.0
25	37.74826557790147	40.0	36.0	41.0	33.0	41.0
26	37.61864723050669	40.0	36.0	41.0	33.0	41.0
27	37.579496339771296	40.0	36.0	41.0	33.0	41.0
28	37.492878901452926	40.0	35.0	41.0	33.0	41.0
29	37.453437744214604	40.0	35.0	41.0	33.0	41.0
30	37.28536386979474	39.0	35.0	41.0	33.0	41.0
31	37.14797212165675	39.0	35.0	41.0	32.0	41.0
32	37.0065804373136	39.0	35.0	41.0	32.0	41.0
33	36.83770593770434	39.0	35.0	41.0	31.0	41.0
34	36.610143378893476	39.0	35.0	41.0	30.0	41.0
35	36.40961388175627	39.0	35.0	41.0	30.0	41.0
36	36.22878741965838	39.0	35.0	41.0	30.0	41.0
37	36.171722939028086	39.0	35.0	41.0	29.0	41.0
38	35.992185132613514	39.0	35.0	41.0	29.0	41.0
39	35.886132597566224	39.0	35.0	40.0	28.0	41.0
40	35.775700547040714	39.0	35.0	40.0	27.0	41.0
41	35.63999936205164	39.0	35.0	40.0	26.0	41.0
42	35.539905264668825	39.0	35.0	40.0	26.0	41.0
43	35.47426994784772	38.0	35.0	40.0	26.0	41.0
44	35.311768552335685	38.0	34.0	40.0	25.0	41.0
45	35.1832506658586	38.0	34.0	40.0	25.0	41.0
46	35.067319500486434	38.0	34.0	40.0	24.0	41.0
47	35.0084751439371	38.0	34.0	40.0	24.0	41.0
48	34.882633450822155	38.0	34.0	40.0	24.0	41.0
49	34.76963365815537	38.0	34.0	40.0	24.0	41.0
50	34.61456755075677	38.0	34.0	40.0	24.0	41.0
51	32.887446771183875	36.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	18.0
10	17.0
11	14.0
12	19.0
13	10.0
14	9.0
15	16.0
16	41.0
17	44.0
18	124.0
19	222.0
20	352.0
21	608.0
22	1029.0
23	1661.0
24	2643.0
25	3534.0
26	4162.0
27	4639.0
28	4972.0
29	5293.0
30	6249.0
31	7981.0
32	10598.0
33	15589.0
34	21450.0
35	27707.0
36	36347.0
37	59634.0
38	59904.0
39	38609.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.668203058962376	18.123793878885504	21.014337889347857	17.193665172804263
2	33.77043428334476	23.791008117892858	25.031817674359257	17.40673992440312
3	31.22725315385719	23.912537280107173	28.597311047670694	16.26289851836494
4	27.468461428047398	25.31283392609368	28.45887625396724	18.759828391891674
5	22.08322036331159	36.2252595652382	24.98779923765171	16.703720833798506
6	26.43562303631521	33.617326677405465	26.830194095787945	13.11685619049138
7	48.71979713242213	43.176344874882375	5.954291000143538	2.1495669925519527
8	85.31698059042121	3.7007384252244777	8.8282483532958	2.1540326310585156
9	79.64785250633962	6.036267364156871	10.554855584440439	3.761024545063077
10	40.69568268448669	28.997942616545192	17.672126441364572	12.634248257603547
11	30.358686464330713	25.69145627661441	27.050605253504727	16.89925200555015
12	27.092071896779956	23.863734230714023	29.95167541187541	19.09251846063061
13	24.186217125723672	25.119216599416276	32.06073268368926	18.633833591170795
14	21.336501810178465	25.820321844946655	32.41702684167716	20.426149503197717
15	20.514186376612813	25.599910687229873	33.99148338941963	19.89441954673769
16	25.328782635045695	25.536115851421826	31.233951611617037	17.90114990191544
17	25.352067750115626	24.515398478493168	30.110524553037433	20.022009218353773
18	25.69305114750961	23.895312674439005	30.82375081737133	19.58788536068005
19	25.07200842091833	25.618092215435162	28.959984689239405	20.349914674407106
20	26.44359739079122	26.240410838742605	29.170188673226903	18.14580309723928
21	25.977576115213473	25.764820337793655	29.813559592350998	18.444043954641874
22	25.895599751200137	23.505207253472832	30.436516164016524	20.162676831310506
23	23.623865648075785	26.205642653227223	30.525828934147782	19.64466276454921
24	22.60346724932617	24.864356230363153	32.417983764214284	20.114192756096394
25	23.110636194000094	26.271032359930462	30.4974402322132	20.120891213856236
26	22.74509178482002	26.936093523229292	29.622813033284956	20.69600165866573
27	21.917672764389724	26.302610803655448	30.869364124974087	20.91035230698075
28	20.149917864148897	27.396054289405274	31.24990032056905	21.204127525876782
29	22.06918549943382	26.046474537886162	30.736351892314318	21.147988070365702
30	22.539991387697167	25.47774357665747	30.900304620340986	21.081960415304383
31	23.515095453023076	25.593850177828102	30.199518348989653	20.69153602015917
32	23.875855249517553	25.145691456276616	30.07575636752205	20.902696926683785
33	22.677469258863496	25.242021658346758	29.997607693657198	22.08290138913255
34	21.402529465239787	26.15173601696943	31.888167652828503	20.557566864962283
35	21.998692205865936	27.288878965247765	30.331573659112294	20.380855169774005
36	23.305848391572702	28.41740961069201	27.976268321079406	20.300473676655876
37	22.52850831725172	27.83528173394364	28.31820864101051	21.318001307794134
38	21.85292100604456	28.40528859188849	29.654391477009934	20.087398925057016
39	22.679064129758697	27.254748728090462	28.266534824005994	21.799652318144847
40	24.562606656991118	26.35875025916652	28.74116840241782	20.33747468142454
41	21.520868885663706	26.474218911979076	28.989330313711104	23.015581888646114
42	22.36678840847833	26.398303057367507	28.84387808806877	22.391030446085388
43	21.737133379052967	26.228927768297154	29.29107988708314	22.742858965566736
44	22.010494250490424	26.96703401859619	29.024098499226486	21.998373231686898
45	21.768073874419866	26.56385065628937	28.117892856573263	23.550182612717503
46	21.814963078738774	27.049648330967607	28.729047383614297	22.40634120667932
47	21.11194398813416	27.21998054257508	30.08596354125134	21.582111928039424
48	21.844627677389518	27.673242850991215	29.205913781279406	21.276215690339868
49	22.51255960829971	27.102917018867323	29.055676942951468	21.3288464298815
50	21.753401062184015	27.552989585493055	29.492033619878473	21.201575732444457
51	22.137445973748427	26.972137605460837	27.754581266646465	23.13583515414427
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	138.0
1	388.0
2	638.0
3	3050.5
4	5463.0
5	3690.5
6	1918.0
7	1683.0
8	1448.0
9	1404.5
10	1361.0
11	1363.5
12	1366.0
13	1366.5
14	1367.0
15	1372.0
16	1377.0
17	1376.5
18	1376.0
19	1404.5
20	1433.0
21	1524.0
22	1615.0
23	1790.5
24	1966.0
25	2277.5
26	3188.0
27	3787.0
28	4549.5
29	5312.0
30	6005.0
31	6698.0
32	7511.0
33	8324.0
34	9354.0
35	10384.0
36	11198.0
37	12012.0
38	13051.0
39	14090.0
40	15092.0
41	16094.0
42	17065.5
43	18037.0
44	19339.5
45	20642.0
46	20673.0
47	20704.0
48	20889.0
49	21074.0
50	20623.5
51	20173.0
52	19513.0
53	18853.0
54	18414.0
55	17975.0
56	17223.0
57	16471.0
58	15184.5
59	13898.0
60	13049.0
61	12200.0
62	11069.5
63	9939.0
64	8761.0
65	7583.0
66	6471.5
67	5360.0
68	4455.5
69	3551.0
70	2975.5
71	2400.0
72	1987.0
73	1574.0
74	1310.0
75	844.0
76	642.0
77	480.0
78	318.0
79	238.0
80	158.0
81	118.5
82	79.0
83	57.0
84	35.0
85	26.0
86	17.0
87	12.5
88	8.0
89	5.0
90	2.0
91	3.5
92	5.0
93	3.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	313505.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.471759939781414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.78486401600223	31.70395032178604
2	11.26182763640421	10.69237556077153
3	6.4644568905777025	9.206374369517114
4	4.485116365636305	8.516654696458863
5	3.2747582926946364	7.772926976580413
6	2.3928308172554944	6.815513407995829
7	1.6424514486749962	5.457904261897194
8	1.1718327260203232	4.4503169487373135
9	0.8029295897674297	3.4304832660587934
>10	1.700538984707565	10.111781260379445
>50	0.01089969319060228	0.3573484490690647
>100	0.006131077419713783	0.4886457072790559
>500	0.0	0.0
>1k	0.001362461648825285	0.9957247734693666
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1951	0.6223186233074433	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1128	0.35980287395735316	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	460	0.14672812235849508	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012758967161608268	0.0
2	0.0	0.0	0.0	0.04912202357219183	0.0
3	0.0	0.0	0.0	0.07527790625348878	0.0
4	0.0	0.0	0.0	0.10143378893478573	0.0
5	0.0	0.0	0.0	0.2057383454809333	0.0
6	0.0	0.0	0.0	0.3486387776909459	0.0
7	0.0	0.0	0.0	0.46346948214542033	0.0
8	0.0	0.0	0.0	0.7310888183601537	0.0
9	0.0	0.0	0.0	0.8727133538540055	0.0
10	0.0	0.0	0.0	1.1195993684311254	0.0
11	0.0	0.0	0.0	1.3674423055453662	0.0
12	0.0	0.0	0.0	1.5687150125197364	0.0
13	0.0	0.0	0.0	1.6720626465287636	0.0
14	0.0	0.0	0.0	1.7479785011403326	0.0
15	0.0	0.0	0.0	1.8267651233632638	0.0
16	0.0	0.0	0.0	1.9578635109487887	0.0
17	0.0	0.0	0.0	2.095660356294158	0.0
18	0.0	0.0	0.0	2.2535525749190604	0.0
19	0.0	0.0	0.0	2.3581761056442483	0.0
20	0.0	0.0	0.0	2.482895009648969	0.0
21	0.0	0.0	0.0	2.642382099169072	0.0
22	0.0	0.0	0.0	2.791980989138929	0.0
23	0.0	0.0	0.0	2.951787052838073	0.0
24	0.0	0.0	0.0	3.0825664662445575	0.0
25	0.0	0.0	0.0	3.2056904993540773	0.0
26	0.0	0.0	0.0	3.323710945598954	0.0
27	0.0	0.0	0.0	3.4650165069137655	0.0
28	0.0	0.0	0.0	3.601537455542974	0.0
29	0.0	0.0	0.0	3.7667660802858007	0.0
30	0.0	0.0	0.0	4.025773113666449	0.0
31	0.0	0.0	0.0	4.209502240793608	0.0
32	0.0	0.0	0.0	4.417473405527822	0.0
33	0.0	0.0	0.0	4.610452783847148	0.0
34	0.0	0.0	0.0	4.779190124559417	0.0
35	0.0	0.0	0.0	4.992902824516356	0.0
36	0.0	0.0	0.0	5.177907848359675	0.0
37	0.0	0.0	0.0	5.350153905041387	0.0
38	0.0	0.0	0.0	5.557487121417521	0.0
39	0.0	0.0	0.0	5.923988453134719	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAGGC	25	3.8842936E-5	45.000004	4
GCGATCT	40	6.7884685E-9	45.000004	9
TAGGCCG	20	7.0254295E-4	45.000004	6
AGTACGG	20	7.0254295E-4	45.000004	2
CGAAGGA	45	3.8198777E-10	45.000004	4
CGAATAT	30	2.1604337E-6	44.999996	14
CGTTTAG	30	2.1604337E-6	44.999996	2
GACCGAT	145	0.0	43.448273	9
CGTTATT	140	0.0	43.392857	1
CGTTTTA	480	0.0	43.124996	1
AGGCGAT	145	0.0	41.89655	7
GCCGATG	45	1.920671E-8	40.000004	9
CGTTTTT	770	0.0	39.44805	1
GTAGGAC	40	3.4474942E-7	39.375004	5
ACACGAC	190	0.0	39.07895	26
TACGAAT	35	6.233762E-6	38.571426	12
TAAGGTC	35	6.233762E-6	38.571426	5
GCTACGA	35	6.233762E-6	38.571426	10
CACGACC	200	0.0	38.250004	27
ACGACCA	200	0.0	38.250004	28
>>END_MODULE