##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934691.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	400913
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.961679965478794	33.0	31.0	34.0	30.0	34.0
2	32.143811749681355	33.0	31.0	34.0	30.0	34.0
3	32.051031021692985	33.0	31.0	34.0	30.0	34.0
4	35.80181236328081	37.0	35.0	37.0	35.0	37.0
5	32.10207700922644	37.0	35.0	37.0	0.0	37.0
6	33.949235869128714	37.0	35.0	37.0	19.0	37.0
7	20.75338290352271	32.0	0.0	37.0	0.0	37.0
8	28.072085464926307	35.0	17.0	37.0	17.0	37.0
9	34.81473786083265	37.0	32.0	39.0	32.0	39.0
10	36.18843988596029	37.0	35.0	39.0	32.0	39.0
11	36.597371499552274	38.0	35.0	39.0	32.0	39.0
12	36.909137393898426	39.0	37.0	39.0	33.0	39.0
13	36.66491732620294	39.0	35.0	39.0	33.0	39.0
14	37.95438162394335	40.0	37.0	41.0	33.0	41.0
15	38.14741352862092	40.0	37.0	41.0	33.0	41.0
16	38.25603310443912	40.0	37.0	41.0	33.0	41.0
17	38.23664984672485	40.0	37.0	41.0	34.0	41.0
18	38.14480198945906	40.0	37.0	41.0	33.0	41.0
19	38.10244367231793	40.0	37.0	41.0	34.0	41.0
20	38.08560460748342	40.0	37.0	41.0	34.0	41.0
21	37.976089575543824	40.0	37.0	41.0	33.0	41.0
22	38.00148161820644	40.0	37.0	41.0	34.0	41.0
23	37.952139242179726	40.0	36.0	41.0	33.0	41.0
24	37.84231990481725	40.0	36.0	41.0	33.0	41.0
25	37.67541336898529	40.0	36.0	41.0	33.0	41.0
26	37.54543753881765	39.0	36.0	41.0	33.0	41.0
27	37.50734947482371	39.0	36.0	41.0	33.0	41.0
28	37.41465105895793	39.0	36.0	41.0	33.0	41.0
29	37.37296121602442	39.0	36.0	41.0	33.0	41.0
30	37.21123784960827	39.0	35.0	41.0	32.0	41.0
31	37.08899686465642	39.0	35.0	41.0	32.0	41.0
32	37.00223988745688	39.0	35.0	41.0	31.0	41.0
33	36.837979811081205	39.0	35.0	41.0	31.0	41.0
34	36.68769533539696	39.0	35.0	41.0	31.0	41.0
35	36.564306969342475	39.0	35.0	41.0	30.0	41.0
36	36.370150132322976	39.0	35.0	41.0	30.0	41.0
37	36.336686513034	39.0	35.0	41.0	30.0	41.0
38	36.13096606994535	39.0	35.0	40.0	29.0	41.0
39	36.02339410295999	39.0	35.0	40.0	29.0	41.0
40	35.899102797863875	39.0	35.0	40.0	28.0	41.0
41	35.740025890904015	39.0	35.0	40.0	27.0	41.0
42	35.63893163853504	38.0	35.0	40.0	27.0	41.0
43	35.55330957090441	38.0	35.0	40.0	27.0	41.0
44	35.419811779613035	38.0	34.0	40.0	26.0	41.0
45	35.290818706302865	38.0	34.0	40.0	26.0	41.0
46	35.165247821846634	38.0	34.0	40.0	26.0	41.0
47	35.08581662355673	38.0	34.0	40.0	26.0	41.0
48	34.949804571066544	38.0	34.0	40.0	25.0	41.0
49	34.83239006966599	38.0	34.0	40.0	24.0	41.0
50	34.67999541047559	37.0	34.0	40.0	24.0	41.0
51	32.8082177430016	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	22.0
10	27.0
11	16.0
12	13.0
13	7.0
14	9.0
15	17.0
16	45.0
17	106.0
18	179.0
19	303.0
20	536.0
21	849.0
22	1323.0
23	2016.0
24	2842.0
25	3750.0
26	4657.0
27	5322.0
28	6031.0
29	7079.0
30	8676.0
31	11017.0
32	14964.0
33	20848.0
34	29162.0
35	36759.0
36	47080.0
37	75873.0
38	74461.0
39	46914.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.22879776909205	17.91336274952421	22.04243813495696	15.81540134642678
2	31.685926871914855	24.412029542569087	25.840768446021954	18.061275139494104
3	33.05081152269943	24.203256068024732	26.789103870415776	15.956828538860051
4	28.39194538465952	25.7472319430899	26.924794157335878	18.936028514914707
5	22.495404239822605	36.87957237605166	23.02344897770838	17.601574406417352
6	26.630964822791974	35.03378538485906	25.21694232913375	13.118307463215212
7	49.7327350322888	42.92552249490538	4.956187502026625	2.3855549707791965
8	85.52653568230514	4.447348926076232	7.158410927058988	2.8677044645596426
9	79.80833746972536	6.420096130581947	8.928620423882489	4.842945975810213
10	43.160985051619676	27.067717933816066	16.52228787791865	13.249009136645606
11	33.34089939712606	25.586598588721245	23.89396203166273	17.178539982489966
12	29.39964530958088	24.56393282333075	27.383247737040207	18.653174130048164
13	24.98721667793262	25.18052545065887	30.39287825538209	19.43937961602642
14	21.76457236358013	26.609513784786227	31.035162242182217	20.590751609451427
15	20.79129387173776	25.25186262356172	33.21194373841706	20.74489976628346
16	25.34639684919172	25.0390982582256	29.983063657202436	19.631441235380244
17	25.487574610950503	23.86028889060719	28.468271171052073	22.183865327390233
18	26.830758793054855	23.75078882450806	29.268444774801516	20.150007607635573
19	25.900382377223984	25.82605203622731	26.568108292821634	21.70545729372707
20	27.493246664488307	26.072738973293458	27.607735344077145	18.826279018141094
21	26.22888257552137	26.295729996283484	28.528383963603076	18.94700346459207
22	26.366568307837362	22.9234272772397	29.15719869398099	21.55280572094195
23	24.08851795776141	26.22414339270615	29.060419592280617	20.626919057251822
24	23.700902689610963	24.807626592303066	30.64729754335729	20.84417317472868
25	23.94808848802608	26.56087480326156	28.586002449409225	20.90503425930314
26	23.129706445039197	26.558629927191184	27.867642106891022	22.444021520878596
27	22.766285952313844	26.383529593702377	29.36422615380394	21.48595830017984
28	21.63062809138142	27.675081626188224	29.532092997732672	21.16219728469768
29	23.188821514892258	26.11115129716422	29.063662191048934	21.63636499689459
30	23.947839057351594	25.286533487315204	29.5642695547413	21.2013579005919
31	25.459388944733647	25.75820689276726	28.073671844016033	20.70873231848306
32	25.82605203622731	25.608049626727	27.40844023516324	21.157458101882455
33	24.704362293066076	25.969474674056464	27.09091498654321	22.23524804633424
34	23.24070309518524	25.902377822619872	30.11326646928386	20.74365261291103
35	23.542264780638195	27.13880567604443	28.509427232342176	20.8095023109752
36	25.303245342505733	27.900816386597594	26.54141921065169	20.25451906024499
37	24.427743675061674	27.34433655182047	26.912821484960574	21.31509828815728
38	23.049140337180386	27.819003125366354	27.689299174633895	21.442557362819365
39	23.643284203804814	26.984657519212398	27.17472369317034	22.19733458381245
40	26.620987595812558	25.55167829429327	26.920803266544112	20.906530843350055
41	22.46921401900163	25.68337769042161	28.345800709879697	23.50160758069706
42	23.426279517002442	25.0682816471404	28.434099168647563	23.071339667209596
43	23.21251742896838	25.574625916345937	28.316866751639385	22.895989903046296
44	23.566459556063286	25.90462269869024	28.19140312237318	22.337514622873293
45	23.300317026387273	26.14657045294116	27.210641710296247	23.342470810375318
46	23.2207486412264	26.657903335636412	26.721508157630208	23.39983986550698
47	21.783529094841025	26.908581163494322	29.258966409171066	22.048923332493583
48	22.91893752509896	26.91381920765852	28.53037940899896	21.63686385824356
49	23.72135600491877	26.382282440329945	28.63089997081661	21.26546158393467
50	22.136224068563504	27.42739696642414	28.61942615979028	21.81695280522208
51	22.928415890729408	26.221150224612323	27.11037057915308	23.740063305505185
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	213.0
1	641.0
2	1069.0
3	2181.5
4	3294.0
5	2290.0
6	1286.0
7	1217.0
8	1148.0
9	1113.0
10	1078.0
11	1078.0
12	1078.0
13	1131.5
14	1185.0
15	1240.0
16	1295.0
17	1225.0
18	1155.0
19	1220.5
20	1286.0
21	1429.0
22	1572.0
23	1796.0
24	2020.0
25	2560.5
26	3671.5
27	4242.0
28	5231.0
29	6220.0
30	6867.0
31	7514.0
32	8739.0
33	9964.0
34	11313.5
35	12663.0
36	13667.0
37	14671.0
38	15842.5
39	17014.0
40	18414.5
41	19815.0
42	21268.5
43	22722.0
44	23686.5
45	24651.0
46	24813.5
47	24976.0
48	26687.0
49	28398.0
50	28545.5
51	28693.0
52	28202.0
53	27711.0
54	26583.0
55	25455.0
56	24264.5
57	23074.0
58	21783.5
59	20493.0
60	18870.0
61	17247.0
62	15458.5
63	13670.0
64	12008.5
65	10347.0
66	8857.0
67	7367.0
68	6179.0
69	4991.0
70	4089.5
71	3188.0
72	2660.5
73	2133.0
74	1720.5
75	1025.0
76	742.0
77	569.5
78	397.0
79	319.0
80	241.0
81	179.5
82	118.0
83	88.5
84	59.0
85	40.5
86	22.0
87	15.5
88	9.0
89	6.0
90	3.0
91	5.0
92	7.0
93	5.0
94	3.0
95	3.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	400913.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.33815211160195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.53250456575947	27.24136441651058
2	9.197646211268864	7.420321038777256
3	5.140507747531077	6.2207575035233065
4	3.6880939932417074	5.950835860050777
5	2.837880966638841	5.723743705344877
6	2.3200197517121413	5.6151185787891205
7	1.8822258911015828	5.314786001255581
8	1.5327662575432615	4.946316675864875
9	1.270268000229687	4.611623743418899
>10	4.558488873489946	25.032541401708087
>50	0.02702702990141492	0.7284193798992279
>100	0.011313640423848106	0.76746448414722
>500	6.285355791026724E-4	0.157448115181838
>1k	6.285355791026724E-4	0.2692590955283606
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1062	0.26489537630358706	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	621	0.15489644885548737	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014716409794643726	0.0
2	0.0	0.0	0.0	0.0912916268616882	0.0
3	0.0	0.0	0.0	0.11648412498472237	0.0
4	0.0	0.0	0.0	0.16512310650939233	0.0
5	0.0	0.0	0.0	0.3793840558924255	0.0
6	0.0	0.0	0.0	0.5205618176512111	0.0
7	0.0	0.0	0.0	0.6495174763602078	0.0
8	0.0	0.0	0.0	0.9044356256843754	0.0
9	0.0	0.0	0.0	0.9992192819888605	0.0
10	0.0	0.0	0.0	1.3618914826907584	0.0
11	0.0	0.0	0.0	1.642500991486931	0.0
12	0.0	0.0	0.0	1.956534210664159	0.0
13	0.0	0.0	0.0	2.075762073068222	0.0
14	0.0	0.0	0.0	2.14385664720276	0.0
15	0.0	0.0	0.0	2.238640303507245	0.0
16	0.0	0.0	0.0	2.4217224185795923	0.0
17	0.0	0.0	0.0	2.6185232207486413	0.0
18	0.0	0.0	0.0	2.8497454559966875	0.0
19	0.0	0.0	0.0	2.994914108547241	0.0
20	0.0	0.0	0.0	3.148563404030301	0.0
21	0.0	0.0	0.0	3.346860790246263	0.0
22	0.0	0.0	0.0	3.5678563678404043	0.0
23	0.0	0.0	0.0	3.7808701638510103	0.0
24	0.0	0.0	0.0	3.951480745199083	0.0
25	0.0	0.0	0.0	4.117601574406417	0.0
26	0.0	0.0	0.0	4.271250869889477	0.0
27	0.0	0.0	0.0	4.460319321149476	0.0
28	0.0	0.0	0.0	4.645147450943222	0.0
29	0.0	0.0	0.0	4.827231843317628	0.0
30	0.0	0.0	0.0	5.131287835515436	0.0
31	0.0	0.0	0.0	5.352532843784063	0.0
32	0.0	0.0	0.0	5.579015896216885	0.0
33	0.0	0.0	0.0	5.787789370761238	0.0
34	0.0	0.0	0.0	5.9925719545138225	0.0
35	0.0	0.0	0.0	6.247988965186961	0.0
36	0.0	0.0	0.0	6.464993651989334	0.0
37	0.0	0.0	0.0	6.6862386602579615	0.0
38	0.0	0.0	0.0	6.947891437793237	0.0
39	0.0	0.0	0.0	7.362694649462601	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	60	0.0	45.000004	4
TATGCGG	30	2.1619526E-6	45.000004	2
ACTAGGT	30	2.1619526E-6	45.000004	4
CTATCGT	20	7.027785E-4	45.0	33
CATTCGA	25	3.8862454E-5	45.0	15
TCGTGCG	20	7.027785E-4	45.0	45
ATACCGG	25	3.8862454E-5	45.0	2
AGGCGAT	140	0.0	41.785713	7
CGTTTTA	245	0.0	41.32653	1
TATGGGC	100	0.0	40.5	4
CGCACGG	40	3.4507138E-7	39.375	2
GTACGGG	160	0.0	39.375	3
CGTTTTT	425	0.0	38.647057	1
CAATTAG	70	0.0	38.571426	2
TAGGCAC	35	6.238117E-6	38.571426	6
CGCATAG	65	9.094947E-12	38.07692	2
AACGTTC	30	1.138596E-4	37.500004	40
CGAACGT	30	1.138596E-4	37.500004	38
AACGGGC	30	1.138596E-4	37.500004	4
CTGCACG	30	1.138596E-4	37.500004	1
>>END_MODULE