##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934687.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	216631
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.926340182153062	31.0	31.0	34.0	30.0	34.0
2	32.10645752454635	33.0	31.0	34.0	30.0	34.0
3	31.91183625612216	33.0	31.0	34.0	30.0	34.0
4	35.724097659153124	37.0	35.0	37.0	35.0	37.0
5	32.01695048261791	37.0	35.0	37.0	0.0	37.0
6	33.92630325299703	37.0	35.0	37.0	19.0	37.0
7	20.80964404909731	32.0	0.0	37.0	0.0	37.0
8	28.177176858344374	35.0	17.0	37.0	17.0	37.0
9	34.96312623770375	37.0	32.0	39.0	32.0	39.0
10	36.22347678771736	37.0	35.0	39.0	32.0	39.0
11	36.582617446256535	38.0	35.0	39.0	32.0	39.0
12	37.063795117042346	39.0	37.0	39.0	34.0	39.0
13	36.916678591706635	39.0	37.0	39.0	33.0	39.0
14	38.31602125272929	40.0	38.0	41.0	34.0	41.0
15	38.49630016018021	40.0	38.0	41.0	34.0	41.0
16	38.559749066384775	40.0	38.0	41.0	34.0	41.0
17	38.528206950990395	40.0	38.0	41.0	34.0	41.0
18	38.42637480323684	40.0	38.0	41.0	34.0	41.0
19	38.372190499051385	40.0	37.0	41.0	34.0	41.0
20	38.339891335958384	40.0	37.0	41.0	34.0	41.0
21	38.24805314105553	40.0	37.0	41.0	34.0	41.0
22	38.25730851078562	40.0	37.0	41.0	34.0	41.0
23	38.17739381713605	40.0	37.0	41.0	34.0	41.0
24	38.05834345038337	40.0	37.0	41.0	34.0	41.0
25	37.9421135479225	40.0	36.0	41.0	33.0	41.0
26	37.823894087180506	40.0	36.0	41.0	33.0	41.0
27	37.79405071296352	40.0	36.0	41.0	33.0	41.0
28	37.760343625796864	40.0	36.0	41.0	33.0	41.0
29	37.76325179683425	40.0	36.0	41.0	33.0	41.0
30	37.645733990056826	40.0	36.0	41.0	33.0	41.0
31	37.53694069639156	40.0	36.0	41.0	33.0	41.0
32	37.45018949273188	40.0	36.0	41.0	33.0	41.0
33	37.29685963689408	40.0	36.0	41.0	32.0	41.0
34	37.1619712783489	40.0	36.0	41.0	32.0	41.0
35	37.008558331910024	40.0	36.0	41.0	31.0	41.0
36	36.84625930730135	40.0	35.0	41.0	31.0	41.0
37	36.785076928048156	40.0	35.0	41.0	31.0	41.0
38	36.6945727989069	40.0	35.0	41.0	30.0	41.0
39	36.63494144420697	39.0	35.0	41.0	30.0	41.0
40	36.5840299864747	39.0	35.0	41.0	30.0	41.0
41	36.50966389851868	39.0	35.0	41.0	30.0	41.0
42	36.420826197543285	39.0	35.0	41.0	30.0	41.0
43	36.3454030124959	39.0	35.0	41.0	30.0	41.0
44	36.209803767697146	39.0	35.0	40.0	29.0	41.0
45	36.12902123888086	39.0	35.0	40.0	29.0	41.0
46	36.03048040215851	39.0	35.0	40.0	28.0	41.0
47	36.0268059511335	39.0	35.0	40.0	28.0	41.0
48	35.94045173590114	39.0	35.0	40.0	28.0	41.0
49	35.834169624845934	39.0	35.0	40.0	28.0	41.0
50	35.70187553951189	39.0	35.0	40.0	27.0	41.0
51	33.97893653262922	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	7.0
11	4.0
12	4.0
13	2.0
14	5.0
15	5.0
16	13.0
17	30.0
18	44.0
19	123.0
20	186.0
21	279.0
22	533.0
23	822.0
24	1354.0
25	1861.0
26	2350.0
27	2472.0
28	2880.0
29	3235.0
30	3851.0
31	4860.0
32	6654.0
33	9644.0
34	13623.0
35	18743.0
36	23788.0
37	41851.0
38	46031.0
39	31364.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.06181479105022	18.998665934238407	24.16043871837364	17.779080556337735
2	30.27313727028911	25.566054719776947	26.78194718207459	17.378860827859356
3	29.911693155642542	24.114738887786142	29.676269785949376	16.297298170621932
4	26.817029880303373	26.654079979319672	28.92337661738163	17.605513522995324
5	21.41752565422308	37.67420175321168	24.688525649606934	16.219746942958302
6	24.942413597315248	35.032382253694074	27.064916840156766	12.960287308833914
7	50.14286967239222	42.617169287867384	5.157618254081826	2.0823427856585623
8	87.5262543218653	3.4801113414054314	7.089013114466535	1.9046212222627417
9	81.5834298876892	7.306433520594929	8.394920394588032	2.7152161971278352
10	34.8883585451759	41.05414275888493	14.876910506806503	9.180588189132672
11	24.43048317184521	25.787168041508373	33.86819060983885	15.914158176807566
12	24.49741726715013	24.656212638080422	33.78879292437371	17.057577170395742
13	21.074546117591662	27.041836117637825	35.05500136176263	16.828616403007878
14	17.90279322903924	30.299910908411075	32.34901745364237	19.448278408907314
15	17.678910220605545	29.181880709593734	34.821886064321355	18.317323005479363
16	20.35719726170308	28.639022115948315	34.07083935355512	16.932941268793478
17	20.299495455405737	27.246792933605995	32.39425566977949	20.059455941208785
18	20.885745807386755	27.40004893113174	32.679533400113556	19.03467186136795
19	21.062544141881816	29.139873794609265	30.84323111650687	18.954350947002045
20	22.390147301171115	28.67179674192521	31.482567130281446	17.45548882662223
21	21.403677220711717	28.740577295031645	32.43210805471055	17.423637429546094
22	21.444299292345047	26.622690196693917	32.045275145293154	19.887735365667886
23	19.71601479012699	28.632097899192637	32.138521264269656	19.513366046410717
24	18.25177375352558	27.833966514487766	35.01991866353384	18.894341068452807
25	18.48211936426458	30.08341373118344	32.085897216926476	19.348569687625503
26	19.154691618466423	30.94155499443755	30.757370828736423	19.146382558359605
27	18.67784389122517	30.326222932082665	31.925255388194675	19.07067778849749
28	17.809085495612354	29.721508002086498	33.5824512650544	18.886955237246745
29	17.979421227802113	27.250024234758648	33.7149346123131	21.055619925126138
30	19.550295202441017	28.855980907626332	32.179143335902985	19.414580554029666
31	20.237639119054982	28.98984909823617	30.971559933712168	19.800951848996682
32	20.39828094778679	28.95061186995398	30.513638398936436	20.13746878332279
33	19.97544211123985	28.969076447969126	31.070345426093223	19.985136014697805
34	18.557362519676314	29.651804220079303	30.792915141415587	20.997918118828792
35	18.691230710286156	28.886909075801707	31.86755358189733	20.55430663201481
36	20.329962009130735	29.870609469558833	29.91584768569595	19.883580835614477
37	18.754010275537667	29.730278676643696	31.817699221256422	19.69801182656222
38	18.375948040677464	30.431009412318645	31.26653156750419	19.926510979499703
39	18.495967797775943	30.804455502675054	31.336696963961757	19.362879735587242
40	21.137787297293553	29.712275713078924	29.755667471414526	19.394269518212997
41	18.168221538007025	29.252969334952063	31.390244240205696	21.188564886835216
42	19.603380864234573	28.88413938909944	30.31375934192244	21.19872040474355
43	19.812030595805773	28.73365307827596	30.330377462136077	21.12393886378219
44	19.21516311146604	30.282831173747066	29.31621051465395	21.185795200132944
45	18.697693312591458	30.658585336355372	29.56548231785848	21.078239033194695
46	19.647695851470935	30.80584034602619	28.807511390336565	20.738952412166313
47	18.698154927041834	29.64580323222438	31.097119064215185	20.558922776518596
48	19.47966819153307	28.751194427390352	31.24114277273336	20.52799460834322
49	19.399347277167163	28.93814827979375	31.08234740180307	20.58015704123602
50	18.276700933846033	29.54055513753803	31.057420221482612	21.12532370713333
51	18.527357580401695	29.11494661428881	29.75151294136112	22.606182863948373
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	131.0
1	274.5
2	418.0
3	1770.5
4	3123.0
5	2161.5
6	1200.0
7	1063.0
8	926.0
9	953.0
10	980.0
11	997.0
12	1014.0
13	1014.0
14	1014.0
15	1023.5
16	1033.0
17	1082.5
18	1132.0
19	1166.5
20	1201.0
21	1270.5
22	1340.0
23	1553.0
24	1766.0
25	2234.0
26	3237.0
27	3772.0
28	4605.0
29	5438.0
30	6247.0
31	7056.0
32	8009.5
33	8963.0
34	9660.0
35	10357.0
36	11196.0
37	12035.0
38	12772.5
39	13510.0
40	14484.0
41	15458.0
42	16246.5
43	17035.0
44	17716.0
45	18397.0
46	18100.0
47	17803.0
48	17078.0
49	16353.0
50	15001.5
51	13650.0
52	12305.0
53	10960.0
54	9666.0
55	8372.0
56	7308.0
57	6244.0
58	5326.5
59	4409.0
60	3900.0
61	3391.0
62	2829.0
63	2267.0
64	1796.0
65	1325.0
66	1041.5
67	758.0
68	589.0
69	420.0
70	345.5
71	271.0
72	214.0
73	157.0
74	127.0
75	75.0
76	53.0
77	49.0
78	45.0
79	33.0
80	21.0
81	17.5
82	14.0
83	13.5
84	13.0
85	7.5
86	2.0
87	1.5
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	216631.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.04546328133254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.2279328347078	32.780426417711766
2	11.35590797431758	10.91199719212533
3	6.09875531963662	8.79052574314298
4	3.9516912988320234	7.594433567887809
5	2.753754824136412	6.615271314441914
6	2.0495667245858264	5.908342968523759
7	1.4772449069716056	4.968244115480784
8	1.1376482782700188	4.37270708645547
9	0.8300137937815514	3.589055752691793
>10	2.104499472788318	13.109483214485183
>50	0.0079905058290659	0.23130430153181966
>100	0.002996439685899712	0.26345655921362865
>500	9.988132286332374E-4	0.3104852346288119
>1k	9.988132286332374E-4	0.5542665316789456
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1155	0.5331646901874616	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	647	0.29866454939505427	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	291	0.1343298050602176	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.035082698228785354	0.0
2	0.0	0.0	0.0	0.13063688945718757	0.0
3	0.0	0.0	0.0	0.18603062350263813	0.0
4	0.0	0.0	0.0	0.26081216446399635	0.0
5	0.0	0.0	0.0	0.4348408122567869	0.0
6	0.0	0.0	0.0	0.6148704479045012	0.0
7	4.6161445037875464E-4	0.0	0.0	0.7699729032317628	0.0
8	4.6161445037875464E-4	0.0	0.0	1.2422044859692287	0.0
9	4.6161445037875464E-4	0.0	0.0	1.418541206013913	0.0
10	4.6161445037875464E-4	0.0	0.0	1.819222548942672	0.0
11	4.6161445037875464E-4	0.0	0.0	2.3394620345195287	0.0
12	4.6161445037875464E-4	0.0	0.0	2.7604544132649527	0.0
13	4.6161445037875464E-4	0.0	0.0	2.957102169126302	0.0
14	4.6161445037875464E-4	0.0	0.0	3.049886673652432	0.0
15	4.6161445037875464E-4	0.0	0.0	3.1671367440486358	0.0
16	4.6161445037875464E-4	0.0	0.0	3.413177246100512	0.0
17	4.6161445037875464E-4	0.0	0.0	3.7529254815792754	0.0
18	4.6161445037875464E-4	0.0	0.0	4.1217554274319	0.0
19	4.6161445037875464E-4	0.0	0.0	4.382567591895897	0.0
20	4.6161445037875464E-4	0.0	0.0	4.6175293471386825	0.0
21	4.6161445037875464E-4	0.0	0.0	4.9300423300451	0.0
22	4.6161445037875464E-4	0.0	0.0	5.256865360913258	0.0
23	4.6161445037875464E-4	0.0	0.0	5.643698270330654	0.0
24	4.6161445037875464E-4	0.0	0.0	5.926667928412831	4.6161445037875464E-4
25	4.6161445037875464E-4	0.0	0.0	6.173170044915086	4.6161445037875464E-4
26	4.6161445037875464E-4	0.0	0.0	6.396129824448025	4.6161445037875464E-4
27	4.6161445037875464E-4	0.0	0.0	6.6486329288052035	4.6161445037875464E-4
28	4.6161445037875464E-4	0.0	0.0	6.912214779971472	4.6161445037875464E-4
29	4.6161445037875464E-4	0.0	0.0	7.199800582557437	4.6161445037875464E-4
30	4.6161445037875464E-4	0.0	0.0	7.515544866616505	4.6161445037875464E-4
31	4.6161445037875464E-4	0.0	0.0	7.831289150675572	4.6161445037875464E-4
32	4.6161445037875464E-4	0.0	0.0	8.152572808139187	4.6161445037875464E-4
33	4.6161445037875464E-4	0.0	0.0	8.457238345389165	4.6161445037875464E-4
34	4.6161445037875464E-4	0.0	0.0	8.755441280333839	4.6161445037875464E-4
35	4.6161445037875464E-4	0.0	0.0	9.072108793293665	4.6161445037875464E-4
36	4.6161445037875464E-4	0.0	0.0	9.38554500510084	4.6161445037875464E-4
37	4.6161445037875464E-4	0.0	0.0	9.73037099953377	4.6161445037875464E-4
38	4.6161445037875464E-4	0.0	0.0	10.066426319409503	4.6161445037875464E-4
39	4.6161445037875464E-4	0.0	0.0	10.432024964109477	4.6161445037875464E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAC	40	6.7702786E-9	45.000004	26
ACGCCGG	20	7.020599E-4	45.000004	27
ATAGGCG	40	6.7702786E-9	45.000004	5
GAACTTA	20	7.020599E-4	45.000004	9
GGCCGAT	20	7.020599E-4	45.000004	8
TAAACGG	20	7.020599E-4	45.000004	2
CACGCCG	20	7.020599E-4	45.000004	26
TCTACGG	20	7.020599E-4	45.000004	2
GACGGGC	20	7.020599E-4	45.000004	4
CGAGACA	40	6.7702786E-9	45.000004	22
CGCCGGT	20	7.020599E-4	45.000004	28
ATCATCC	20	7.020599E-4	45.000004	35
CCACCTT	20	7.020599E-4	45.000004	24
TGGTACC	20	7.020599E-4	45.000004	19
ACACGCG	25	3.880294E-5	45.0	36
CGCAGGA	35	1.2058263E-7	45.0	4
TAGGCGA	35	1.2058263E-7	45.0	6
AGGCGAT	130	0.0	44.999996	7
TATGCGG	30	2.1573214E-6	44.999996	2
CGTTTTT	605	0.0	42.396694	1
>>END_MODULE