FastQCFastQC Report
Sat 14 Jan 2017
SRR2934684.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934684.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327465
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14450.44126853251492526No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7770.23727726627273144No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCGC253.8846752E-545.0000049
CGATTGA253.8846752E-545.00000410
TCGATCA253.8846752E-545.00000417
CGTTATT1000.045.0000041
CGTTTAG253.8846752E-545.0000042
CTCACGA207.0258905E-445.024
CCGATTA207.0258905E-445.011
GTCCGCA207.0258905E-445.010
CGCACGG207.0258905E-445.02
CACGGGC302.1607302E-644.9999964
CGTTTTA2100.042.8571471
CGTTGAT1300.041.5384625
TTAATCG1350.040.00000420
ACGGGCG451.9210347E-840.05
ATAGGCG403.448131E-739.3755
TCTCGTA403.448131E-739.37528
ACGGTTA403.448131E-739.37534
GGACCGA1150.039.1304328
AGGCGAT1500.039.07
CGAAGGA750.039.04
Produced by FastQC (version 0.11.5)