##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934684.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	327465
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.976620402180387	33.0	31.0	34.0	30.0	34.0
2	32.1667720214374	33.0	31.0	34.0	30.0	34.0
3	32.02384682332463	33.0	31.0	34.0	30.0	34.0
4	35.79312903669094	37.0	35.0	37.0	35.0	37.0
5	32.04722947490571	37.0	35.0	37.0	0.0	37.0
6	33.94459560563725	37.0	35.0	37.0	19.0	37.0
7	20.71353274395737	32.0	0.0	37.0	0.0	37.0
8	28.07057242758768	35.0	17.0	37.0	17.0	37.0
9	34.840660833982255	37.0	32.0	39.0	32.0	39.0
10	36.26992197639442	37.0	35.0	39.0	32.0	39.0
11	36.655828867207184	38.0	35.0	39.0	33.0	39.0
12	36.98420594567359	39.0	37.0	39.0	33.0	39.0
13	36.74152352159773	39.0	35.0	39.0	33.0	39.0
14	38.07997190539447	40.0	37.0	41.0	33.0	41.0
15	38.26213183088269	40.0	37.0	41.0	33.0	41.0
16	38.34627517444613	40.0	38.0	41.0	34.0	41.0
17	38.30951399386194	40.0	37.0	41.0	34.0	41.0
18	38.2149725924908	40.0	37.0	41.0	34.0	41.0
19	38.15738170491503	40.0	37.0	41.0	34.0	41.0
20	38.130383399752645	40.0	37.0	41.0	34.0	41.0
21	38.04959919380697	40.0	37.0	41.0	34.0	41.0
22	38.05826882262227	40.0	37.0	41.0	34.0	41.0
23	38.001575740918874	40.0	36.0	41.0	34.0	41.0
24	37.8758004672255	40.0	36.0	41.0	33.0	41.0
25	37.71667506451071	40.0	36.0	41.0	33.0	41.0
26	37.58954697448582	39.0	36.0	41.0	33.0	41.0
27	37.56064312216573	39.0	36.0	41.0	33.0	41.0
28	37.480472722275664	39.0	36.0	41.0	33.0	41.0
29	37.459951445192615	40.0	36.0	41.0	33.0	41.0
30	37.302236880277285	39.0	35.0	41.0	33.0	41.0
31	37.1606767135419	39.0	35.0	41.0	32.0	41.0
32	37.05629303895073	39.0	35.0	41.0	32.0	41.0
33	36.92348495259035	39.0	35.0	41.0	31.0	41.0
34	36.750999343441286	39.0	35.0	41.0	31.0	41.0
35	36.604757760371335	39.0	35.0	41.0	30.0	41.0
36	36.42632342387736	39.0	35.0	41.0	30.0	41.0
37	36.33279892507596	39.0	35.0	41.0	30.0	41.0
38	36.18215687172675	39.0	35.0	41.0	29.0	41.0
39	36.06679797840991	39.0	35.0	40.0	29.0	41.0
40	35.927552562869316	39.0	35.0	40.0	28.0	41.0
41	35.78103003374407	39.0	35.0	40.0	27.0	41.0
42	35.67201685676332	39.0	35.0	40.0	27.0	41.0
43	35.60591513596873	38.0	35.0	40.0	27.0	41.0
44	35.45716336096987	38.0	35.0	40.0	26.0	41.0
45	35.329329852045255	38.0	34.0	40.0	26.0	41.0
46	35.204794405508984	38.0	34.0	40.0	26.0	41.0
47	35.12761363809873	38.0	34.0	40.0	25.0	41.0
48	35.014410700380196	38.0	34.0	40.0	25.0	41.0
49	34.90430122303147	38.0	34.0	40.0	24.0	41.0
50	34.751484891515126	38.0	34.0	40.0	24.0	41.0
51	32.89556441146382	36.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	13.0
9	17.0
10	6.0
11	7.0
12	4.0
13	8.0
14	10.0
15	12.0
16	41.0
17	60.0
18	120.0
19	195.0
20	347.0
21	595.0
22	959.0
23	1518.0
24	2292.0
25	3134.0
26	3942.0
27	4593.0
28	4953.0
29	5634.0
30	6850.0
31	8782.0
32	11738.0
33	17179.0
34	23657.0
35	30054.0
36	37453.0
37	61596.0
38	62292.0
39	39401.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.11869970836578	17.97108087887255	22.162368497396667	17.747850915365003
2	30.651214633624967	24.47040141694532	26.566808666575053	18.311575282854655
3	32.09197929549723	24.19067686622998	27.480188722458887	16.237155115813902
4	27.737010062144048	25.60578993174843	27.64509184187623	19.012108164231293
5	22.48698334173118	37.01586429084024	23.597636388621684	16.899515978806896
6	26.295329271830575	34.27633487548288	25.94903272105416	13.479303131632388
7	49.17411021025148	42.989632479807	5.526697509657521	2.3095598002839997
8	85.2338417846182	4.398027270089933	7.911379842120532	2.456751103171331
9	79.32969935718322	6.8581375108790255	9.512772357351167	4.299390774586597
10	42.36391675446231	28.626570778556488	16.81614829065702	12.193364176324186
11	31.69254729513078	24.529644389476736	26.41350984074634	17.364298474646144
12	28.03108729177164	23.320660223229964	29.711266852946117	18.93698563205228
13	23.610767562945657	26.02904127158628	31.25891316629258	19.101277999175483
14	20.85413708335242	26.882567602644556	31.868749331989676	20.394545982013344
15	20.19116546806529	25.984456354114183	33.74467500343548	20.07970317438505
16	24.302444536057287	25.00847418808117	31.483059258241337	19.206022017620203
17	24.328706884705237	24.78402271998534	29.290763898431894	21.59650649687753
18	25.360572885651898	24.196173636877223	30.287206266318538	20.156047211152337
19	24.71348083001237	26.02415525323317	27.94161208068038	21.320751836074084
20	26.00247354679126	26.130426152413232	29.160062907486296	18.707037393309207
21	25.28331272044341	25.93437466599484	30.110698853312567	18.671613760249187
22	24.780358206220512	23.890186737513933	30.323851403966835	21.005603652298717
23	23.138656039576748	26.242804574534684	29.82395065121463	20.794588734673937
24	22.326050112225733	25.339807307651203	31.827218175988275	20.506924404134793
25	22.758768112622725	26.42053349212893	29.733864687829232	21.086833707419114
26	22.496755378437392	27.313453346159132	28.74414059517811	21.445650680225366
27	21.625822606996167	26.296550776418854	30.857038156749578	21.2205884598354
28	19.619195944604765	27.3336081718657	30.835356450307668	22.211839433221872
29	21.65300108408532	26.529552776632613	30.360496541615134	21.456949597666927
30	22.04266104774556	25.779243583283712	31.042096101873483	21.135999267097247
31	23.770784664009895	25.582275968424106	29.270914448872396	21.376024918693602
32	24.135709159757532	25.97712732658452	29.132579054250073	20.754584459407877
33	23.137434534988472	25.224985876353195	28.809185714503837	22.82839387415449
34	21.00926816606355	25.60243079413067	31.16638419373063	22.221916846075153
35	21.534515139022492	26.64803872169545	30.57334371612233	21.244102423159728
36	23.54175255370803	27.097247034034172	28.140106576275326	21.220893835982473
37	21.622768845525474	28.018261493594736	28.202097934130364	22.156871726749426
38	22.253675965370345	28.139495823981186	27.532408043607713	22.074420167040753
39	22.16939214877926	27.646313346464506	27.881452979707756	22.30284152504848
40	24.3161864626754	25.52303299589269	28.19934954880674	21.961430992625168
41	21.010795046798894	25.130930023055896	28.774067457590885	25.084207472554322
42	21.361061487487213	25.739239308017652	28.556639640877652	24.343059563617487
43	22.383155451727667	25.9496434733483	28.976226466950667	22.69097460797337
44	21.88447620356374	26.491380758248972	28.4928160261402	23.13132701204709
45	20.858412349411388	27.100911547799	28.041164704624922	23.99951139816469
46	21.34793031316324	27.312537217717924	27.839921823706348	23.499610645412485
47	20.647702807933673	27.225199639656143	29.599499183118805	22.527598369291375
48	21.229749744247478	26.95891163941185	29.72470340341716	22.086635212923518
49	22.205121157986348	26.452597987571192	28.897744797153894	22.444536057288563
50	21.159513230421574	27.130227657917636	29.509107843586335	22.20115126807445
51	20.903913395324693	26.89233963935077	27.95504863115142	24.24869833417312
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	188.0
1	543.5
2	899.0
3	2472.0
4	4045.0
5	2768.5
6	1492.0
7	1324.0
8	1156.0
9	1171.5
10	1187.0
11	1168.5
12	1150.0
13	1148.5
14	1147.0
15	1084.5
16	1022.0
17	1053.5
18	1085.0
19	1137.5
20	1190.0
21	1268.0
22	1346.0
23	1642.0
24	1938.0
25	2537.5
26	3530.5
27	3924.0
28	4742.0
29	5560.0
30	6405.5
31	7251.0
32	8051.5
33	8852.0
34	10043.0
35	11234.0
36	11994.5
37	12755.0
38	13579.5
39	14404.0
40	15671.5
41	16939.0
42	18018.0
43	19097.0
44	20071.5
45	21046.0
46	21453.5
47	21861.0
48	22674.5
49	23488.0
50	23121.5
51	22755.0
52	21928.5
53	21102.0
54	19884.0
55	18666.0
56	17801.0
57	16936.0
58	16123.5
59	15311.0
60	14094.5
61	12878.0
62	11398.5
63	9919.0
64	8758.5
65	7598.0
66	6495.0
67	5392.0
68	4517.5
69	3643.0
70	2948.0
71	2253.0
72	1875.0
73	1497.0
74	1242.5
75	797.5
76	607.0
77	450.5
78	294.0
79	213.0
80	132.0
81	90.5
82	49.0
83	36.0
84	23.0
85	18.0
86	13.0
87	9.0
88	5.0
89	5.0
90	5.0
91	3.5
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	327465.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.306204692966716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.93281876243958	29.852187593893852
2	9.590802795275225	8.306825380441325
3	5.285092724989012	6.866319221090501
4	3.6331190207996062	6.293463839546343
5	2.6622643821194867	5.764628313943052
6	2.1584025279265733	5.6083333010522995
7	1.7147061211860375	5.198018999065588
8	1.3030475765057323	4.514403605826516
9	1.0203921066787152	3.977037849502437
>10	3.672920432985087	21.856506172908816
>50	0.016431665653630183	0.5039935309561007
>100	0.008573042949720095	0.570821402464092
>500	7.144202458100079E-4	0.24039470445235742
>1k	7.144202458100079E-4	0.4470660848567007
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1445	0.44126853251492526	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	777	0.23727726627273144	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.021070954147771517	0.0
2	0.0	0.0	0.0	0.10749240376834165	0.0
3	0.0	0.0	0.0	0.13772464232818774	0.0
4	0.0	0.0	0.0	0.19941062403615653	0.0
5	0.0	0.0	0.0	0.39454598201334495	0.0
6	0.0	0.0	0.0	0.5191394500175591	0.0
7	0.0	0.0	0.0	0.6293802391095231	0.0
8	0.0	0.0	0.0	0.8657413769410471	0.0
9	0.0	0.0	0.0	0.9573542210617929	0.0
10	0.0	0.0	0.0	1.3470141847220314	0.0
11	0.0	0.0	0.0	1.6832333226451681	0.0
12	0.0	0.0	0.0	2.0429664238926297	0.0
13	0.0	0.0	0.0	2.16908677263219	0.0
14	0.0	0.0	0.0	2.235658772693265	0.0
15	0.0	0.0	0.0	2.3504802039912662	0.0
16	0.0	0.0	0.0	2.5569144794100134	0.0
17	0.0	0.0	0.0	2.784419708976532	0.0
18	0.0	0.0	0.0	3.042767929397035	0.0
19	0.0	0.0	0.0	3.2162215809323134	0.0
20	3.0537614706915243E-4	0.0	0.0	3.3939504985265603	0.0
21	3.0537614706915243E-4	0.0	0.0	3.622066480387217	0.0
22	3.0537614706915243E-4	0.0	0.0	3.8773609393370285	0.0
23	3.0537614706915243E-4	0.0	0.0	4.115554334050968	0.0
24	3.0537614706915243E-4	0.0	0.0	4.325347747087475	0.0
25	3.0537614706915243E-4	0.0	0.0	4.512237949093796	0.0
26	3.0537614706915243E-4	0.0	0.0	4.700044279541325	0.0
27	3.0537614706915243E-4	0.0	0.0	4.926938756813705	0.0
28	3.0537614706915243E-4	0.0	0.0	5.139480555173836	0.0
29	3.0537614706915243E-4	0.0	0.0	5.386529858152779	0.0
30	3.0537614706915243E-4	0.0	0.0	5.7001511611927995	0.0
31	3.0537614706915243E-4	0.0	0.0	5.947505840318812	0.0
32	3.0537614706915243E-4	0.0	0.0	6.19363901485655	0.0
33	3.0537614706915243E-4	0.0	0.0	6.43824530865894	0.0
34	3.0537614706915243E-4	0.0	0.0	6.686210740079092	0.0
35	3.0537614706915243E-4	0.0	0.0	6.955247125647015	0.0
36	3.0537614706915243E-4	0.0	0.0	7.206266318537859	0.0
37	3.0537614706915243E-4	0.0	0.0	7.460950025193532	0.0
38	3.0537614706915243E-4	0.0	0.0	7.7495304841738815	0.0
39	3.0537614706915243E-4	0.0	0.0	8.181637732276732	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCCGC	25	3.8846752E-5	45.000004	9
CGATTGA	25	3.8846752E-5	45.000004	10
TCGATCA	25	3.8846752E-5	45.000004	17
CGTTATT	100	0.0	45.000004	1
CGTTTAG	25	3.8846752E-5	45.000004	2
CTCACGA	20	7.0258905E-4	45.0	24
CCGATTA	20	7.0258905E-4	45.0	11
GTCCGCA	20	7.0258905E-4	45.0	10
CGCACGG	20	7.0258905E-4	45.0	2
CACGGGC	30	2.1607302E-6	44.999996	4
CGTTTTA	210	0.0	42.857147	1
CGTTGAT	130	0.0	41.53846	25
TTAATCG	135	0.0	40.000004	20
ACGGGCG	45	1.9210347E-8	40.0	5
ATAGGCG	40	3.448131E-7	39.375	5
TCTCGTA	40	3.448131E-7	39.375	28
ACGGTTA	40	3.448131E-7	39.375	34
GGACCGA	115	0.0	39.130432	8
AGGCGAT	150	0.0	39.0	7
CGAAGGA	75	0.0	39.0	4
>>END_MODULE