##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934683.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	322228
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.944045830902343	33.0	31.0	34.0	30.0	34.0
2	32.133402435542536	33.0	31.0	34.0	30.0	34.0
3	31.95632905892722	33.0	31.0	34.0	30.0	34.0
4	35.751433767394516	37.0	35.0	37.0	35.0	37.0
5	32.05687587670842	37.0	35.0	37.0	0.0	37.0
6	33.937668359050114	37.0	35.0	37.0	19.0	37.0
7	20.804250406544433	32.0	0.0	37.0	0.0	37.0
8	28.119052348026862	35.0	17.0	37.0	17.0	37.0
9	34.85388917164244	37.0	32.0	39.0	32.0	39.0
10	36.238343657286144	37.0	35.0	39.0	32.0	39.0
11	36.658061993371156	38.0	35.0	39.0	33.0	39.0
12	36.990388793028536	39.0	37.0	39.0	33.0	39.0
13	36.762180816068124	39.0	37.0	39.0	33.0	39.0
14	38.13749581041995	40.0	37.0	41.0	33.0	41.0
15	38.33314609531139	40.0	38.0	41.0	34.0	41.0
16	38.398488027111235	40.0	38.0	41.0	34.0	41.0
17	38.3845289670668	40.0	38.0	41.0	34.0	41.0
18	38.25092170761076	40.0	37.0	41.0	34.0	41.0
19	38.18526943654803	40.0	37.0	41.0	34.0	41.0
20	38.163238452276026	40.0	37.0	41.0	34.0	41.0
21	38.06253336147076	40.0	37.0	41.0	34.0	41.0
22	38.03199908139578	40.0	37.0	41.0	34.0	41.0
23	37.96617612373847	40.0	36.0	41.0	34.0	41.0
24	37.80023151309011	40.0	36.0	41.0	33.0	41.0
25	37.665693235845424	40.0	36.0	41.0	33.0	41.0
26	37.512081507504	39.0	35.0	41.0	33.0	41.0
27	37.493231500676536	39.0	36.0	41.0	33.0	41.0
28	37.40583686085629	39.0	35.0	41.0	33.0	41.0
29	37.389559566518116	39.0	36.0	41.0	33.0	41.0
30	37.25021413409139	39.0	35.0	41.0	32.0	41.0
31	37.097654455851135	39.0	35.0	41.0	32.0	41.0
32	36.99336184316695	39.0	35.0	41.0	31.0	41.0
33	36.82709758307782	39.0	35.0	41.0	31.0	41.0
34	36.63743684596	39.0	35.0	41.0	30.0	41.0
35	36.48082724033914	39.0	35.0	41.0	30.0	41.0
36	36.2711154834465	39.0	35.0	41.0	30.0	41.0
37	36.19199138498206	39.0	35.0	41.0	30.0	41.0
38	36.058117233759944	39.0	35.0	40.0	29.0	41.0
39	35.962687910423675	39.0	35.0	40.0	28.0	41.0
40	35.84159042665442	39.0	35.0	40.0	28.0	41.0
41	35.71021140310587	39.0	35.0	40.0	27.0	41.0
42	35.596822746626614	38.0	35.0	40.0	27.0	41.0
43	35.50386682721551	38.0	35.0	40.0	26.0	41.0
44	35.3884702757054	38.0	34.0	40.0	26.0	41.0
45	35.279417058728605	38.0	34.0	40.0	26.0	41.0
46	35.13405725138722	38.0	34.0	40.0	25.0	41.0
47	35.0819947366461	38.0	34.0	40.0	25.0	41.0
48	34.999460009682586	38.0	34.0	40.0	25.0	41.0
49	34.85807564829872	38.0	34.0	40.0	24.0	41.0
50	34.698725746986604	37.0	34.0	40.0	24.0	41.0
51	32.84483036855891	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	10.0
10	10.0
11	17.0
12	7.0
13	12.0
14	13.0
15	19.0
16	38.0
17	68.0
18	116.0
19	232.0
20	404.0
21	662.0
22	1054.0
23	1631.0
24	2484.0
25	3390.0
26	4142.0
27	4531.0
28	4885.0
29	5485.0
30	6791.0
31	8592.0
32	11340.0
33	16197.0
34	23016.0
35	29505.0
36	37364.0
37	60066.0
38	61339.0
39	38794.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.64925456509056	18.46580682001564	23.67298931191579	18.211949302978013
2	31.283749394838438	24.372494010452225	25.595230706208028	18.748525888501312
3	31.12640738855717	23.71736782650794	28.75820847350323	16.398016311431657
4	26.366113435207367	25.703228769691027	27.696537855183283	20.23411993991832
5	22.178705761138076	37.61622205394938	24.00070757351937	16.204364611393174
6	25.660091612150403	35.043819903918966	25.538128281837707	13.757960202092928
7	48.13548170860385	42.77468128157702	6.064960214506499	3.0248767953126356
8	83.6339486326452	4.361197661283315	9.301488387104783	2.703365318966694
9	78.229390369552	6.334024355425351	11.154524125774296	4.282061149248358
10	41.59911615377931	29.359956304231787	16.824112119368895	12.216815422620009
11	30.168700423302752	26.131186613205553	26.621832987822287	17.078279975669403
12	27.802363543826107	23.041448911950543	30.242871507131596	18.91331603709175
13	23.339995282843205	25.70788385863426	30.52434921856574	20.427771639956802
14	20.171741748079	27.462542050970118	30.867274104050544	21.49844209690033
15	19.38565239519843	25.67126382561416	34.76048015690753	20.182603622279878
16	23.37630497660042	24.868416152537954	32.086286728651764	19.668992142209866
17	23.136102387129608	24.180394006728157	29.346301376664968	23.337202229477263
18	24.154325508646053	25.22934071526993	30.577106893255706	20.039226882828306
19	24.249910001613763	26.63641893317775	28.366870663008804	20.746800402199685
20	25.81650260064302	25.672194843402806	29.85680946410616	18.65449309184801
21	24.625110170438322	27.233201335700187	28.830827861017667	19.310860632843823
22	26.099842347654455	23.712402398301823	28.169494891815734	22.018260362227988
23	23.136412726392493	26.702521196171652	29.281130131459708	20.87993594597614
24	21.813126109462864	26.09518725871122	31.665777027446406	20.42590960437951
25	22.01763968370222	27.242821852849534	30.01694452375337	20.722593939694875
26	21.66074953138771	27.590091488014696	29.071340789751353	21.67781819084623
27	21.29299750487233	26.840311828891345	30.43807490348449	21.42861576275184
28	20.52987325744504	26.728279354990875	31.26109462864804	21.480752758916047
29	20.67821542510272	25.69360825254168	30.955100115446204	22.673076206909393
30	21.161723996673164	27.358578397904587	30.334731928944723	21.144965676477526
31	23.14168849386149	27.12427225442854	28.47300669091451	21.261032560795464
32	22.894348101344388	27.389612324192807	27.726330424419977	21.989709150042827
33	22.442494134587932	27.31016547289497	27.73719229862085	22.510148093896248
34	20.96807229663468	27.114651737279193	29.495884901374183	22.421391064711944
35	22.040294449892624	26.911689859354247	28.92858472882555	22.11943096192758
36	22.73110964906836	28.0428764725598	28.249872760902218	20.97614111746962
37	21.525441612771083	27.960946907158906	28.65393448117482	21.859676998895193
38	21.34885857219112	28.958377298062242	27.778157081321304	21.91460704842534
39	22.06419057313455	27.770088260486364	28.3929391610909	21.772782005288178
40	23.836538103454696	26.424767555892103	28.549970828109288	21.188723512543913
41	20.32101493352533	26.96630956962151	29.0462033094579	23.66647218739526
42	21.817470859143214	26.330424419975916	27.734399245254913	24.117705475625957
43	22.10639671288653	26.65876336010527	28.104323646610474	23.130516280397732
44	21.665714959593828	27.812915078764107	28.562384398624573	21.958985563017492
45	21.47268393808111	27.839293916109092	27.15251312735082	23.53550901845898
46	22.213463758580883	28.183770497908313	27.226994550442544	22.37577119306826
47	20.6577330337525	27.523989225020795	30.15225244237	21.666025298856713
48	21.837953250493438	26.721451891207472	29.263130454212543	22.177464404086546
49	21.86495276636419	26.440594858299093	29.42667924575146	22.267773129585265
50	20.23691299328426	27.351130255595418	29.57843514530084	22.83352160581948
51	20.671387961319315	26.90517273483372	28.49038568963591	23.933053614211055
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	266.0
1	632.0
2	998.0
3	2695.0
4	4392.0
5	3035.0
6	1678.0
7	1530.0
8	1382.0
9	1353.0
10	1324.0
11	1331.0
12	1338.0
13	1353.0
14	1368.0
15	1294.0
16	1220.0
17	1235.5
18	1251.0
19	1263.0
20	1275.0
21	1415.5
22	1556.0
23	1683.5
24	1811.0
25	2075.0
26	2803.0
27	3267.0
28	4442.0
29	5617.0
30	6322.5
31	7028.0
32	7633.5
33	8239.0
34	9720.0
35	11201.0
36	11973.0
37	12745.0
38	13741.0
39	14737.0
40	15976.0
41	17215.0
42	19164.0
43	21113.0
44	22728.5
45	24344.0
46	23639.5
47	22935.0
48	22484.0
49	22033.0
50	21042.5
51	20052.0
52	19392.0
53	18732.0
54	17557.0
55	16382.0
56	15669.0
57	14956.0
58	14339.0
59	13722.0
60	13110.0
61	12498.0
62	11257.5
63	10017.0
64	9061.5
65	8106.0
66	6870.0
67	5634.0
68	4667.0
69	3700.0
70	2988.5
71	2277.0
72	1893.5
73	1510.0
74	1208.5
75	715.5
76	524.0
77	378.5
78	233.0
79	194.5
80	156.0
81	111.0
82	66.0
83	50.5
84	35.0
85	28.5
86	22.0
87	14.0
88	6.0
89	8.0
90	10.0
91	8.0
92	6.0
93	4.0
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	322228.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.94112740830558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.22064525962853	29.03191902119204
2	9.090745612575134	7.6255223994701735
3	5.141287048571719	6.468941254404537
4	3.5550556943669376	5.964121752842639
5	2.6461830170920235	5.549194953277551
6	2.0801933145159133	5.234739170281045
7	1.688032761120516	4.9558597972484595
8	1.3118535472931618	4.401649341444811
9	1.1070663960539704	4.178845148971794
>10	4.128208320314735	24.116263492181833
>50	0.01798772398087615	0.5375281140779943
>100	0.01049283898884442	0.952147752948681
>500	0.0014989769984063458	0.49006218119742284
>1k	7.494884992031729E-4	0.49320562046103683
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1569	0.48692230346214477	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	899	0.27899499733108235	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	660	0.20482391350224063	No Hit
GCTCAAATTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	464	0.14399741797733284	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	375	0.11637722358081855	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019241034298695333	0.0
2	0.0	0.0	0.0	0.11513586652928982	0.0
3	0.0	0.0	0.0	0.15082488176074085	0.0
4	0.0	0.0	0.0	0.21258239507429522	0.0
5	0.0	0.0	0.0	0.44812989560187194	0.0
6	0.0	0.0	0.0	0.5989547773626128	0.0
7	0.0	0.0	0.0	0.7441935523914743	0.0
8	0.0	0.0	0.0	1.1013940439688668	0.0
9	0.0	0.0	0.0	1.2283228024876796	0.0
10	0.0	0.0	0.0	1.6385913080179253	0.0
11	0.0	0.0	0.0	1.9988951922241394	0.0
12	0.0	0.0	0.0	2.3908536812443364	0.0
13	0.0	0.0	0.0	2.573022828556178	0.0
14	0.0	0.0	0.0	2.636332038184143	0.0
15	0.0	0.0	0.0	2.780639795424358	0.0
16	0.0	0.0	0.0	2.9761535310401332	0.0
17	0.0	0.0	0.0	3.2411832615415173	0.0
18	3.103392628821828E-4	0.0	0.0	3.50745434909443	0.0
19	3.103392628821828E-4	0.0	0.0	3.6827960326228633	0.0
20	3.103392628821828E-4	0.0	0.0	3.885447571284929	0.0
21	3.103392628821828E-4	0.0	0.0	4.116339982869273	0.0
22	3.103392628821828E-4	0.0	0.0	4.381990391896421	0.0
23	3.103392628821828E-4	0.0	0.0	4.656950978810035	0.0
24	3.103392628821828E-4	0.0	0.0	4.871395409461623	0.0
25	3.103392628821828E-4	0.0	0.0	5.123080551659074	0.0
26	3.103392628821828E-4	0.0	0.0	5.310525466439912	0.0
27	3.103392628821828E-4	0.0	0.0	5.512866665839096	0.0
28	3.103392628821828E-4	0.0	0.0	5.714897525975396	0.0
29	3.103392628821828E-4	0.0	0.0	5.9203421180034015	0.0
30	3.103392628821828E-4	0.0	0.0	6.16613081420609	0.0
31	3.103392628821828E-4	0.0	0.0	6.399816279156374	0.0
32	3.103392628821828E-4	0.0	0.0	6.659570242188761	0.0
33	3.103392628821828E-4	0.0	0.0	6.890772993035987	0.0
34	3.103392628821828E-4	0.0	0.0	7.17845748972777	0.0
35	3.103392628821828E-4	0.0	0.0	7.457762826321735	0.0
36	3.103392628821828E-4	0.0	0.0	7.703861861787306	0.0
37	3.103392628821828E-4	0.0	0.0	7.949029879464231	0.0
38	3.103392628821828E-4	0.0	0.0	8.206611467656442	0.0
39	3.103392628821828E-4	0.0	0.0	8.52191615874474	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGCAA	25	3.8845355E-5	45.000004	1
TGCGACG	25	3.8845355E-5	45.000004	1
CGACTAG	25	3.8845355E-5	45.000004	2
AGTACGG	25	3.8845355E-5	45.000004	2
GCGAATG	20	7.0257223E-4	45.0	1
AGGCGAT	195	0.0	45.0	7
ACCTCGT	20	7.0257223E-4	45.0	17
CGTAGGA	45	3.8380676E-10	45.0	4
CGCTAGC	20	7.0257223E-4	45.0	41
CCTCAAC	20	7.0257223E-4	45.0	33
TGTACGG	40	6.7884685E-9	45.0	2
CGTTTTA	315	0.0	43.571426	1
CACGACC	90	0.0	42.5	27
CGTTTTT	590	0.0	41.18644	1
CGTTATT	105	0.0	40.714287	1
ACGGGAT	150	0.0	40.500004	5
GCGAGAC	95	0.0	40.263157	21
CGACCAA	95	0.0	40.263157	29
TCAAGCG	95	0.0	40.263157	17
AACACGT	90	0.0	40.0	41
>>END_MODULE