##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934682.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	457760
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02596994058022	33.0	31.0	34.0	30.0	34.0
2	32.225231562390775	33.0	31.0	34.0	30.0	34.0
3	32.11145796924152	33.0	31.0	34.0	30.0	34.0
4	35.8549239776302	37.0	35.0	37.0	35.0	37.0
5	32.08606256553652	37.0	35.0	37.0	0.0	37.0
6	33.958742572527086	37.0	35.0	37.0	19.0	37.0
7	20.80765248164977	32.0	0.0	37.0	0.0	37.0
8	28.139544739601536	35.0	17.0	37.0	17.0	37.0
9	34.89990387976232	37.0	32.0	39.0	32.0	39.0
10	36.28431055574974	37.0	35.0	39.0	32.0	39.0
11	36.6465571478504	38.0	35.0	39.0	33.0	39.0
12	36.94753364208319	39.0	37.0	39.0	33.0	39.0
13	36.67522063963649	39.0	35.0	39.0	33.0	39.0
14	37.911667686123735	40.0	37.0	41.0	33.0	41.0
15	38.129179045788185	40.0	37.0	41.0	33.0	41.0
16	38.26192764767564	40.0	37.0	41.0	33.0	41.0
17	38.24484227542817	40.0	37.0	41.0	34.0	41.0
18	38.14401433065362	40.0	37.0	41.0	33.0	41.0
19	38.109830478853546	40.0	37.0	41.0	34.0	41.0
20	38.07553303040895	40.0	37.0	41.0	34.0	41.0
21	37.984952813701504	40.0	37.0	41.0	33.0	41.0
22	37.98685555749738	40.0	36.0	41.0	34.0	41.0
23	37.939433327507864	40.0	36.0	41.0	34.0	41.0
24	37.82868752184551	40.0	36.0	41.0	33.0	41.0
25	37.66845945473611	40.0	36.0	41.0	33.0	41.0
26	37.539131859489686	39.0	35.0	41.0	33.0	41.0
27	37.50607742048235	39.0	35.0	41.0	33.0	41.0
28	37.41316628801118	39.0	35.0	41.0	33.0	41.0
29	37.36172885354771	39.0	35.0	41.0	33.0	41.0
30	37.163279884655715	39.0	35.0	41.0	32.0	41.0
31	37.016231212862635	39.0	35.0	41.0	31.0	41.0
32	36.91703075847606	39.0	35.0	41.0	31.0	41.0
33	36.7625502446697	39.0	35.0	41.0	31.0	41.0
34	36.60166681230339	39.0	35.0	41.0	30.0	41.0
35	36.43494407549808	39.0	35.0	41.0	30.0	41.0
36	36.28133301293254	39.0	35.0	41.0	30.0	41.0
37	36.22333100314575	39.0	35.0	41.0	30.0	41.0
38	36.04576852499126	39.0	35.0	40.0	29.0	41.0
39	35.91265073400909	39.0	35.0	40.0	28.0	41.0
40	35.765892607479906	39.0	35.0	40.0	27.0	41.0
41	35.62184987766515	38.0	35.0	40.0	27.0	41.0
42	35.52664278224397	38.0	35.0	40.0	26.0	41.0
43	35.469044914365604	38.0	34.0	40.0	26.0	41.0
44	35.30894792030759	38.0	34.0	40.0	26.0	41.0
45	35.195381859489686	38.0	34.0	40.0	26.0	41.0
46	35.092432715833624	38.0	34.0	40.0	26.0	41.0
47	35.010828818594895	38.0	34.0	40.0	25.0	41.0
48	34.868090265641385	38.0	34.0	40.0	24.0	41.0
49	34.768601450541766	37.0	34.0	40.0	24.0	41.0
50	34.59429613771409	37.0	34.0	40.0	24.0	41.0
51	32.77800157287662	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	24.0
10	20.0
11	18.0
12	14.0
13	13.0
14	17.0
15	35.0
16	48.0
17	91.0
18	219.0
19	395.0
20	555.0
21	1024.0
22	1611.0
23	2359.0
24	3384.0
25	4510.0
26	5633.0
27	6600.0
28	7166.0
29	8134.0
30	9873.0
31	12322.0
32	16665.0
33	24546.0
34	33031.0
35	41211.0
36	52614.0
37	85806.0
38	85750.0
39	54055.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44809507165327	17.96640160782943	23.369669695910524	15.21583362460678
2	31.80334673191192	24.013238378189445	25.81986193638588	18.36355295351276
3	33.54727368053128	23.436953862285915	26.461246067808457	16.554526389374345
4	28.870587207270187	25.877315623907727	25.77420482348829	19.4778923453338
5	23.046137714085983	37.01634044040546	23.073881509961552	16.86364033554701
6	26.6488989863684	35.36176162181056	24.873951415588955	13.115387976232087
7	50.12910695560993	42.822658161481996	4.624257252708843	2.4239776301992313
8	86.7981038098567	3.949012583012933	6.805749737853898	2.4471338692764766
9	80.9756204124432	6.334323663054876	8.67594372596994	4.014112198531982
10	46.04312303390423	25.253407899335894	16.00773331003146	12.695735756728416
11	35.062696609577074	25.526913666550154	22.94542991960853	16.464959804264243
12	30.829255505068158	23.442852149598043	27.769355120587207	17.958537224746593
13	25.865300594197834	25.090003495281373	29.236499475707795	19.808196434813002
14	21.331265291855996	25.53062740300594	31.976800069905625	21.161307235232435
15	21.239295700803915	24.995630898287313	33.64055400209717	20.124519398811604
16	27.21753757427473	23.73361586857742	29.86936385879063	19.179482698357216
17	26.829342887102413	23.769005592450192	27.418079342887104	21.983572177560294
18	27.066366655015727	23.807016777350576	29.140597693114294	19.9860188745194
19	26.98903355470115	25.949624257252708	26.36578119538623	20.69556099265991
20	28.025602936036353	26.79482698357218	26.657637189793775	18.521932890597693
21	27.499563089828733	25.81855120587207	28.20102236980077	18.480863334498427
22	26.701109751835023	23.43127403005942	28.82361936385879	21.043996854246767
23	25.367222998951416	25.48606256553653	29.523767913317023	19.622946522195036
24	24.521583362460678	24.759044040545263	30.01835022719329	20.70102236980077
25	24.67581265291856	25.467493883257603	28.830391471513455	21.02630199231038
26	23.204954561342188	26.791331702202026	27.530146801817544	22.47356693463824
27	22.783991611324712	25.707357567284166	29.61726668996854	21.89138413142258
28	21.2436648025166	27.553084585809156	28.64055400209717	22.56269660957707
29	23.745412443201676	26.59581440055924	28.23684900384481	21.42192415239427
30	24.606343935686823	25.172142607479902	28.621548409646973	21.5999650471863
31	25.67983222649423	25.212993708493535	28.09463474309682	21.012539321915412
32	27.221251310730516	24.880505068157987	26.928958406151693	20.969285214959804
33	26.197570779447744	25.476232086682977	27.22955260398462	21.096644529884657
34	22.995456134218806	26.177909821740652	29.496679482698358	21.329954561342188
35	23.52018524991262	27.16423453337994	28.557322614470465	20.75825760223698
36	25.256903180706047	28.342581265291855	25.964042292904576	20.436473261097518
37	23.394355120587207	28.986805312827684	26.344591052079693	21.274248514505416
38	24.17598741698707	28.06579867179308	27.06833275078644	20.689881160433416
39	23.655408947920307	27.278923453337995	26.511709192590004	22.553958406151693
40	27.006073051380636	24.380242922055224	27.439051031108004	21.174632995456133
41	22.21644529884656	25.623252359314925	27.544783292555053	24.615519049283467
42	22.875524292205522	25.75978678783642	28.08567808458581	23.279010835372247
43	22.42332226494233	25.584367354072	28.297142607479902	23.69516777350577
44	23.07431842013282	26.149729115693816	27.834891646277526	22.941060817895842
45	23.026913666550158	26.066716183152742	26.423234882908076	24.483135267389024
46	23.021233834323663	26.970901782593497	26.66135092624956	23.346513456833275
47	21.32121635791681	26.87740300594198	29.257908074099966	22.543472562041245
48	22.84909122684376	27.086682977979727	28.662399510660606	21.401826284515906
49	23.926074799021322	26.38959279972038	28.482392520097864	21.201939881160435
50	21.486805312827684	27.48995106606082	28.373820342537577	22.649423278573924
51	21.932235232436213	26.917817196784345	26.982261447046486	24.16768612373296
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	248.0
1	711.5
2	1175.0
3	2702.5
4	4230.0
5	2883.0
6	1536.0
7	1359.0
8	1182.0
9	1174.5
10	1167.0
11	1179.0
12	1191.0
13	1187.0
14	1183.0
15	1257.0
16	1331.0
17	1289.5
18	1248.0
19	1340.5
20	1433.0
21	1727.5
22	2022.0
23	2193.0
24	2364.0
25	2789.5
26	3981.0
27	4747.0
28	5544.0
29	6341.0
30	7219.5
31	8098.0
32	9684.5
33	11271.0
34	12845.0
35	14419.0
36	15479.5
37	16540.0
38	17546.5
39	18553.0
40	20076.0
41	21599.0
42	23481.5
43	25364.0
44	26218.5
45	27073.0
46	28213.0
47	29353.0
48	29939.5
49	30526.0
50	30659.0
51	30792.0
52	30230.5
53	29669.0
54	29046.5
55	28424.0
56	27976.5
57	27529.0
58	26764.0
59	25999.0
60	24234.0
61	22469.0
62	20225.5
63	17982.0
64	15346.5
65	12711.0
66	10838.0
67	8965.0
68	7639.0
69	6313.0
70	5013.5
71	3714.0
72	3052.0
73	2390.0
74	1934.5
75	1174.5
76	870.0
77	682.5
78	495.0
79	384.5
80	274.0
81	204.0
82	134.0
83	100.5
84	67.0
85	49.0
86	31.0
87	26.0
88	21.0
89	16.0
90	11.0
91	7.5
92	4.0
93	3.5
94	3.0
95	2.0
96	1.0
97	1.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	457760.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.94337160597023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.8536336195628	26.42449268861989
2	8.736583610678556	6.804640442345683
3	4.896163652573951	5.7201936169749565
4	3.4686600443505586	5.403252683276998
5	2.624125811323199	5.109615330558873
6	2.1738639780795594	5.0794555631511
7	1.8696002993350134	5.096597744814562
8	1.5383811661416256	4.792779953974332
9	1.3356330662104783	4.681264934399048
>10	5.4775086038703815	29.260697288251574
>50	0.016082047565974845	0.43616566120212835
>100	0.008615382624629296	0.6775106604006342
>500	5.74358841641953E-4	0.18520264998736385
>1k	5.74358841641953E-4	0.32813078204282947
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1467	0.3204736106256554	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	828	0.18088081090527788	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00917511359664453	0.0
2	0.0	0.0	0.0	0.04259874169870675	0.0
3	0.0	0.0	0.0	0.06291506466270534	0.0
4	0.0	0.0	0.0	0.09044040545263894	0.0
5	0.0	0.0	0.0	0.1732348829080741	0.0
6	0.0	0.0	0.0	0.27328731212862634	0.0
7	0.0	0.0	0.0	0.3558633344984271	0.0
8	0.0	0.0	0.0	0.5719154141908424	0.0
9	0.0	0.0	0.0	0.6678171967843411	0.0
10	0.0	0.0	0.0	0.8895491087032507	0.0
11	0.0	0.0	0.0	1.1414278224397063	0.0
12	0.0	0.0	0.0	1.3391296749388326	0.0
13	0.0	0.0	0.0	1.4278224397063963	0.0
14	0.0	0.0	0.0	1.475882558545963	0.0
15	0.0	0.0	0.0	1.548628102062216	0.0
16	0.0	0.0	0.0	1.6976144704648723	0.0
17	0.0	0.0	0.0	1.8492222998951415	0.0
18	0.0	0.0	0.0	2.03359839217057	0.0
19	0.0	0.0	0.0	2.1428259349877665	0.0
20	0.0	0.0	0.0	2.279578818594897	0.0
21	0.0	0.0	0.0	2.4381772107654665	0.0
22	0.0	0.0	0.0	2.608790632645928	0.0
23	0.0	0.0	0.0	2.785739252009787	0.0
24	0.0	0.0	0.0	2.9238028661307234	0.0
25	0.0	0.0	0.0	3.079124432016777	0.0
26	0.0	0.0	0.0	3.2228678783642084	0.0
27	0.0	0.0	0.0	3.3849615519049285	0.0
28	0.0	0.0	0.0	3.542467668647326	0.0
29	0.0	0.0	0.0	3.7163579168123033	0.0
30	0.0	0.0	0.0	4.058895491087032	0.0
31	0.0	0.0	0.0	4.269704648724223	0.0
32	0.0	0.0	0.0	4.464785040195736	0.0
33	0.0	0.0	0.0	4.650253407899336	0.0
34	0.0	0.0	0.0	4.861062565536526	0.0
35	0.0	0.0	0.0	5.10791681230339	0.0
36	0.0	0.0	0.0	5.306492485145054	0.0
37	0.0	0.0	0.0	5.5103110800419435	0.0
38	0.0	0.0	0.0	5.796705697308633	0.0
39	0.0	0.0	0.0	6.337600489339392	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCACGC	25	3.887114E-5	45.000004	25
ATTGCGG	50	2.1827873E-11	45.000004	2
TCGCCTG	20	7.028834E-4	45.0	40
CCCCTCG	20	7.028834E-4	45.0	20
CTCGTAC	45	3.8380676E-10	45.0	29
CACGGCC	20	7.028834E-4	45.0	25
GCACGCG	20	7.028834E-4	45.0	1
TCTCGTA	45	3.8380676E-10	45.0	28
CACGTAT	20	7.028834E-4	45.0	21
TAGTACG	20	7.028834E-4	45.0	1
CGGCTAG	30	2.1626292E-6	44.999996	2
CGTTTTA	275	0.0	43.363636	1
ATAGGCG	50	1.0786607E-9	40.500004	5
TCGTACG	50	1.0786607E-9	40.500004	30
CACGACC	150	0.0	40.5	27
CGTTATT	145	0.0	40.34483	1
TACGGTT	45	1.9239451E-8	40.0	33
ATAGCGG	40	3.4521508E-7	39.375	2
TCGTCCC	195	0.0	39.23077	38
TGGGCGA	155	0.0	39.193546	6
>>END_MODULE