##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934680.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	123103
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.782637303721273	31.0	31.0	34.0	30.0	34.0
2	31.96685702216843	33.0	31.0	34.0	30.0	34.0
3	31.81534162449331	33.0	31.0	34.0	30.0	34.0
4	35.64670235493855	37.0	35.0	37.0	33.0	37.0
5	31.958798729519184	37.0	35.0	37.0	0.0	37.0
6	33.82571505162344	37.0	35.0	37.0	19.0	37.0
7	20.848777040364574	32.0	0.0	37.0	0.0	37.0
8	28.100363110565947	35.0	17.0	37.0	17.0	37.0
9	34.704986880904606	35.0	32.0	39.0	30.0	39.0
10	36.13006181815228	37.0	35.0	39.0	32.0	39.0
11	36.43858395002559	37.0	35.0	39.0	32.0	39.0
12	36.74964866818843	39.0	35.0	39.0	33.0	39.0
13	36.38292324313786	39.0	35.0	39.0	32.0	39.0
14	37.563186924770314	39.0	36.0	41.0	33.0	41.0
15	37.789818282251446	40.0	36.0	41.0	33.0	41.0
16	37.926321860555795	40.0	37.0	41.0	33.0	41.0
17	37.93385214007782	40.0	36.0	41.0	33.0	41.0
18	37.8230099997563	40.0	36.0	41.0	33.0	41.0
19	37.77157339788632	40.0	36.0	41.0	33.0	41.0
20	37.73220798843245	39.0	36.0	41.0	33.0	41.0
21	37.644931480142645	39.0	36.0	41.0	33.0	41.0
22	37.596248669813086	39.0	35.0	41.0	33.0	41.0
23	37.54220449542253	39.0	35.0	41.0	33.0	41.0
24	37.411833992672804	39.0	35.0	41.0	33.0	41.0
25	37.218175024166754	39.0	35.0	41.0	32.0	41.0
26	37.106569295630486	39.0	35.0	40.0	32.0	41.0
27	37.07080249872058	39.0	35.0	40.0	32.0	41.0
28	36.96224299976443	39.0	35.0	40.0	32.0	41.0
29	36.92338935688001	39.0	35.0	40.0	32.0	41.0
30	36.673817859840945	39.0	35.0	40.0	31.0	41.0
31	36.5164212082565	39.0	35.0	40.0	30.0	41.0
32	36.415603194073256	39.0	35.0	40.0	30.0	41.0
33	36.26174016880174	39.0	35.0	40.0	30.0	41.0
34	36.091159435594584	38.0	35.0	40.0	30.0	41.0
35	35.852310666677496	38.0	35.0	40.0	29.0	41.0
36	35.64796146316499	38.0	35.0	40.0	27.0	41.0
37	35.56529897727919	38.0	34.0	40.0	27.0	41.0
38	35.36371168858598	38.0	34.0	40.0	26.0	41.0
39	35.3024702891075	38.0	34.0	40.0	26.0	41.0
40	35.171458047326226	38.0	34.0	40.0	25.0	41.0
41	35.01445943640691	38.0	34.0	40.0	24.0	41.0
42	34.952974338562015	38.0	34.0	40.0	24.0	41.0
43	34.871440988440575	38.0	34.0	40.0	24.0	41.0
44	34.67869182716912	38.0	34.0	40.0	23.0	41.0
45	34.56885697342876	37.0	33.0	40.0	23.0	41.0
46	34.43721111589482	37.0	33.0	40.0	23.0	41.0
47	34.332209613088224	37.0	33.0	40.0	23.0	41.0
48	34.1849426902675	37.0	33.0	40.0	23.0	41.0
49	34.05737471873147	37.0	33.0	40.0	22.0	41.0
50	33.794635386627455	37.0	33.0	40.0	21.0	41.0
51	31.789672063231603	35.0	29.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	1.0
12	1.0
13	3.0
14	2.0
15	6.0
16	10.0
17	20.0
18	49.0
19	85.0
20	181.0
21	294.0
22	511.0
23	770.0
24	1162.0
25	1499.0
26	1867.0
27	2095.0
28	2353.0
29	2651.0
30	3202.0
31	4074.0
32	5213.0
33	7596.0
34	10105.0
35	11817.0
36	14337.0
37	21553.0
38	19882.0
39	11760.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.554486893089525	15.673866599514227	19.02796844918483	17.743678058211415
2	35.01458128559011	22.450305841449843	22.827225981495168	19.70788689146487
3	34.68233917938637	21.552683525178104	27.087885754205825	16.677091541229704
4	28.662989529093526	25.714239295549255	26.836876436804953	18.78589473855227
5	22.683443945314085	37.17781045141061	23.541262195072417	16.597483408202887
6	28.851449599116187	33.150288782564196	25.92706920221278	12.071192416106838
7	49.26443709738999	41.91611902228215	6.3767739210254835	2.4426699593023726
8	81.23766277020056	4.312648757544495	10.505024247987457	3.9446642242674836
9	75.46038683054029	6.714702322445431	12.621138396302284	5.203772450712005
10	43.98024418576314	24.65902536899994	18.545445683695768	12.815284761541148
11	31.347733199028454	25.628132539418207	26.2641852757447	16.75994898580863
12	31.593868549101163	20.557581862342918	28.851449599116187	18.997099989439736
13	26.222756553455234	24.69233081240912	30.546777901432133	18.538134732703508
14	20.903633542643153	23.434034913852628	32.264039056724855	23.39829248677936
15	19.62746643055003	25.808469330560584	33.91956329252739	20.64450094636199
16	25.156982364361554	24.131824569669302	30.835154301682334	19.876038764286815
17	24.351153099437056	25.037570164821325	28.30150361892074	22.309773116820875
18	24.315410672363793	23.21470638408487	31.781516291235796	20.68836665231554
19	23.02462165828615	25.455106699268093	28.18046676360446	23.339804878841296
20	24.197623128599627	27.600464651551952	29.332347708829193	18.869564511019227
21	25.622446244202006	25.918133595444466	29.7596321779323	18.699787982421224
22	23.95473709007904	24.282917556842644	29.65727886404068	22.105066489037636
23	23.845072825195164	24.25936004808981	30.933445976133804	20.96212115058122
24	23.784960561481036	22.87921496632901	32.130004955200114	21.205819516989838
25	22.415375742264608	23.84750980885925	31.468770054344734	22.268344394531407
26	21.070160759689042	26.032671827656518	29.948092247954968	22.94907516469948
27	21.893861238150166	24.339780509004655	31.11946906249238	22.646889190352795
28	18.577938799216916	26.596427381948452	31.467957726456707	23.357676092377925
29	21.895485893926224	23.640366197411925	29.513496827859598	24.950651080802256
30	22.68831791264226	25.159419348025637	29.809996506990082	22.342266232342023
31	23.31381038642438	25.37468623835325	29.49806259798705	21.813440777235325
32	24.26667099908207	25.2065343655313	28.06511620350438	22.461678431882245
33	23.358488420265957	23.49658416123084	28.81245786049081	24.332469558012395
34	21.131897679179225	23.929554925550146	31.756334126706903	23.18221326856372
35	21.430834341973796	26.045669073864975	29.537054336612428	22.9864422475488
36	23.03843123238264	26.369787901188435	27.476178484683555	23.11560238174537
37	21.727334021104276	26.089534779818525	29.781565030909075	22.40156616816812
38	21.19932089388561	26.99690503074661	29.69220896322592	22.111565112141864
39	22.684256273202116	26.366538589636324	28.16259555006783	22.786609587093736
40	25.671998245371764	24.371461296637776	28.26494886395945	21.691591594031014
41	20.055563227541164	24.892163472864187	28.382736407723613	26.669536891871036
42	21.36341112726741	23.248824155382078	28.75397025255274	26.633794464797774
43	21.157892171596142	24.401517428494838	29.814870474318255	24.625719925590765
44	21.328481028082173	24.67852123831263	29.108957539621294	24.8840401939839
45	21.05228954615241	24.144821815877762	29.262487510458723	25.54040112751111
46	21.54862188573796	25.254461710924996	28.37217614517924	24.824740258157803
47	20.367497136544195	24.867793636223325	30.21697277889247	24.547736448340007
48	21.81669008878744	24.223617621016547	30.38999861904259	23.569693671153424
49	23.00025182164529	24.05221643664249	30.05694418495081	22.89058755676141
50	22.208232130817283	25.00426472141215	29.179630065879792	23.607873081890773
51	21.214755123758152	24.236614867225008	28.03912171108746	26.509508297929372
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	124.0
1	348.5
2	573.0
3	1229.5
4	1886.0
5	1259.0
6	632.0
7	595.5
8	559.0
9	533.5
10	508.0
11	522.0
12	536.0
13	577.0
14	618.0
15	577.0
16	536.0
17	496.0
18	456.0
19	484.0
20	512.0
21	509.5
22	507.0
23	484.0
24	461.0
25	578.0
26	967.0
27	1239.0
28	1502.5
29	1766.0
30	1881.5
31	1997.0
32	2235.0
33	2473.0
34	2781.5
35	3090.0
36	3175.0
37	3260.0
38	3525.0
39	3790.0
40	4272.0
41	4754.0
42	5421.0
43	6088.0
44	6705.0
45	7322.0
46	8045.0
47	8768.0
48	9187.5
49	9607.0
50	9818.5
51	10030.0
52	9352.0
53	8674.0
54	8220.5
55	7767.0
56	7344.5
57	6922.0
58	6687.5
59	6453.0
60	6029.0
61	5605.0
62	5084.5
63	4564.0
64	4002.0
65	3440.0
66	2852.5
67	2265.0
68	1953.0
69	1641.0
70	1393.0
71	1145.0
72	918.0
73	691.0
74	612.5
75	428.0
76	322.0
77	239.0
78	156.0
79	114.0
80	72.0
81	50.0
82	28.0
83	25.5
84	23.0
85	13.5
86	4.0
87	4.5
88	5.0
89	4.5
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	123103.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.632421630667004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.4659814399581	35.47029723077423
2	9.84958837296846	9.77717846031372
3	5.257041850112113	7.827591529044783
4	3.5368827640386917	7.021762264120289
5	2.7758228448910782	6.888540490483579
6	1.9100148938607835	5.687919871977125
7	1.4321019983960457	4.975508314175934
8	1.0262033748506523	4.074636686352079
9	0.813433935088954	3.6335426431524818
>10	1.8936480138791143	12.246655239921042
>50	0.027823695968837464	1.024345466804221
>100	0.009820127989001457	0.8261374621252123
>500	0.0016366879981669095	0.5458843407553025
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	672	0.5458843407553025	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	403	0.3273681388755757	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	142	0.1153505601000788	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	135	0.10966426488387773	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	129	0.10479029755570539	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0917930513472458	0.0
2	0.0	0.0	0.0	0.4313461085432524	0.0
3	0.0	0.0	0.0	0.5548199475236184	0.0
4	0.0	0.0	0.0	0.7042882789209036	0.0
5	0.0	0.0	0.0	1.1080152392711795	0.0
6	0.0	0.0	0.0	1.3703971471044571	0.0
7	0.0	0.0	0.0	1.6807064003314298	0.0
8	0.0	0.0	0.0	2.4613535007270335	0.0
9	0.0	0.0	0.0	2.6928669488152197	0.0
10	0.0	0.0	0.0	3.2818046676360444	0.0
11	0.0	0.0	0.0	4.1599311145950955	0.0
12	0.0	0.0	0.0	4.740745554535633	0.0
13	0.0	0.0	0.0	5.026684971121743	0.0
14	0.0	0.0	0.0	5.148534154326052	0.0
15	0.0	0.0	0.0	5.387358553406497	0.0
16	0.0	0.0	0.0	5.808144399405376	0.0
17	0.0	0.0	0.0	6.319098640975444	0.0
18	0.0	0.0	0.0	6.7983720949123905	0.0
19	0.0	0.0	0.0	7.142799119436569	0.0
20	0.0	0.0	0.0	7.544089096122759	0.0
21	0.0	0.0	0.0	8.050981698252683	0.0
22	0.0	0.0	0.0	8.531067480077658	0.0
23	0.0	0.0	0.0	8.990845064701917	0.0
24	0.0	0.0	0.0	9.352330974874699	0.0
25	0.0	0.0	0.0	9.754433279448916	0.0
26	0.0	0.0	0.0	10.054994598019544	0.0
27	0.0	0.0	0.0	10.436788705393045	0.0
28	0.0	0.0	0.0	10.766593827932708	0.0
29	0.0	0.0	0.0	11.097211278360398	0.0
30	0.0	0.0	0.0	11.680462701965022	0.0
31	0.0	0.0	0.0	12.03138834959343	0.0
32	0.0	0.0	0.0	12.36850442312535	0.0
33	0.0	0.0	0.0	12.762483448819282	0.0
34	0.0	0.0	0.0	13.148339195632925	0.0
35	0.0	0.0	0.0	13.52119769623811	0.0
36	0.0	0.0	0.0	13.841254884121426	0.0
37	0.0	0.0	0.0	14.173496990325175	0.0
38	0.0	0.0	0.0	14.565851360243048	0.0
39	0.0	0.0	0.0	15.095489143237776	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTATA	35	1.200151E-7	45.000004	7
TCACTTA	35	1.200151E-7	45.000004	5
GGCGATA	35	1.200151E-7	45.000004	8
GCGAGAC	85	0.0	45.0	21
GTTGATT	75	0.0	45.0	26
GGTGTTA	20	7.008741E-4	45.0	13
ATTAGGA	20	7.008741E-4	45.0	4
TTAATCG	75	0.0	45.0	20
ATAGGGT	20	7.008741E-4	45.0	5
CGGTTTT	25	3.8704733E-5	45.0	1
CGTTGAT	75	0.0	45.0	25
TAGCGGG	20	7.008741E-4	45.0	3
TTCGTGA	20	7.008741E-4	45.0	17
GGTCAAG	85	0.0	45.0	15
TATGCTT	40	6.730261E-9	45.0	37
AGGCGAT	45	3.783498E-10	45.0	7
ACGGGCT	20	7.008741E-4	45.0	5
ACGGGAA	25	3.8704733E-5	45.0	5
GCACCTA	20	7.008741E-4	45.0	36
ATCGTTG	75	0.0	45.0	23
>>END_MODULE