FastQCFastQC Report
Sat 14 Jan 2017
SRR2934679.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934679.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425764
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24060.565101793481835No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14020.32929040501310586No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5140.12072415704474779No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG302.162269E-645.0000042
CTCACGA207.028279E-445.024
GTCGATA207.028279E-445.01
TCGATAG253.8866543E-545.02
GCGATAT253.8866543E-545.09
TATCGTG207.028279E-445.01
GCGTATG253.8866543E-545.01
CGTTATT2000.043.8751
CGTTTTA5850.043.8461571
CGATAAG451.9233994E-840.02
CGTTTTT9350.039.946521
AGGCGAT2550.039.7058837
CATACGA2500.039.618
ACATACG2500.039.617
CGAATAT403.451387E-739.37514
CGAAAAG356.2390245E-638.571432
ACGGGTA356.2390245E-638.571435
CGTATGG601.546141E-1037.5000042
ATAGGCC301.1387148E-437.5000045
CGCATAG301.1387148E-437.5000042
Produced by FastQC (version 0.11.5)