##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934679.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	425764
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.995809885288562	33.0	31.0	34.0	30.0	34.0
2	32.18171569226144	33.0	31.0	34.0	30.0	34.0
3	31.96581439482906	33.0	31.0	34.0	30.0	34.0
4	35.78804455050216	37.0	35.0	37.0	35.0	37.0
5	32.001874277768906	37.0	35.0	37.0	0.0	37.0
6	33.93917287511391	37.0	35.0	37.0	18.0	37.0
7	20.933188339079866	32.0	0.0	37.0	0.0	37.0
8	28.20053832639678	35.0	17.0	37.0	17.0	37.0
9	34.9570560216458	37.0	32.0	39.0	32.0	39.0
10	36.32827340968236	37.0	35.0	39.0	32.0	39.0
11	36.67309354478068	38.0	35.0	39.0	33.0	39.0
12	36.99417752557755	39.0	37.0	39.0	33.0	39.0
13	36.71747963660619	39.0	35.0	39.0	33.0	39.0
14	37.974817034789226	40.0	37.0	41.0	33.0	41.0
15	38.19468062118921	40.0	37.0	41.0	33.0	41.0
16	38.31418579306846	40.0	37.0	41.0	34.0	41.0
17	38.30930750368749	40.0	37.0	41.0	34.0	41.0
18	38.19703638635488	40.0	37.0	41.0	34.0	41.0
19	38.116480021796114	40.0	37.0	41.0	34.0	41.0
20	38.06675764038294	40.0	37.0	41.0	34.0	41.0
21	37.98213564322019	40.0	36.0	41.0	33.0	41.0
22	37.98957168760158	40.0	36.0	41.0	34.0	41.0
23	37.94439172875114	40.0	36.0	41.0	34.0	41.0
24	37.78596358546049	40.0	36.0	41.0	33.0	41.0
25	37.632066121137534	39.0	35.0	41.0	33.0	41.0
26	37.53400005636926	39.0	35.0	41.0	33.0	41.0
27	37.48599458855141	39.0	35.0	41.0	33.0	41.0
28	37.4070846760177	39.0	35.0	41.0	33.0	41.0
29	37.352303153859886	39.0	35.0	41.0	33.0	41.0
30	37.17718971073177	39.0	35.0	41.0	32.0	41.0
31	37.00392001202544	39.0	35.0	41.0	32.0	41.0
32	36.87450089721066	39.0	35.0	41.0	31.0	41.0
33	36.687199951146646	39.0	35.0	41.0	31.0	41.0
34	36.4268186131284	39.0	35.0	41.0	30.0	41.0
35	36.210233838464504	39.0	35.0	41.0	30.0	41.0
36	36.02565505773151	39.0	35.0	41.0	29.0	41.0
37	35.926409936020896	39.0	35.0	40.0	28.0	41.0
38	35.74459559756109	39.0	35.0	40.0	27.0	41.0
39	35.614361477250306	39.0	35.0	40.0	26.0	41.0
40	35.508932178389905	39.0	35.0	40.0	26.0	41.0
41	35.34373502691632	38.0	35.0	40.0	25.0	41.0
42	35.24993188714875	38.0	34.0	40.0	24.0	41.0
43	35.199371013049486	38.0	34.0	40.0	24.0	41.0
44	35.04108144418034	38.0	34.0	40.0	24.0	41.0
45	34.948271812553436	38.0	34.0	40.0	24.0	41.0
46	34.84560695596622	38.0	34.0	40.0	23.0	41.0
47	34.76847972116008	38.0	34.0	40.0	23.0	41.0
48	34.617952668614535	38.0	34.0	40.0	23.0	41.0
49	34.51657021260605	38.0	34.0	40.0	23.0	41.0
50	34.36067633712573	37.0	33.0	40.0	23.0	41.0
51	32.534979472195865	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	18.0
10	17.0
11	16.0
12	14.0
13	10.0
14	15.0
15	15.0
16	36.0
17	73.0
18	153.0
19	257.0
20	527.0
21	877.0
22	1549.0
23	2342.0
24	3699.0
25	5104.0
26	6172.0
27	6610.0
28	7075.0
29	7747.0
30	9109.0
31	11630.0
32	15434.0
33	22927.0
34	30810.0
35	37890.0
36	48562.0
37	78900.0
38	78277.0
39	49886.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.53466239512969	18.422412416268166	23.8693266692346	17.173598519367538
2	32.166411439201056	24.385575107336457	26.399131913454404	17.04888154000808
3	31.689151736642835	23.528057797277366	28.767815033680634	16.01497543239917
4	27.31160924831597	25.022547702483067	29.308725021373345	18.357118027827624
5	22.209721817720617	37.058088518522	24.712047049539184	16.020142614218205
6	26.196437463007676	34.11138565026634	26.860185454852925	12.831991431873057
7	49.68339267763362	42.22127751524319	5.884010860476696	2.211318946646499
8	86.59844420852866	3.5557257071992936	7.804793265752859	2.0410368185191796
9	81.13602841010513	5.7870087654193405	9.397459625520241	3.6795031989552895
10	44.286271267650626	26.67416690936763	16.68201163085653	12.357550192125215
11	32.25589763343073	24.44311872304845	26.529955562236356	16.771028081284467
12	29.31671066600276	22.518108623556714	29.901776571058146	18.26340413938238
13	25.167933409118664	23.370223879895903	32.5624054640599	18.899437246925526
14	21.432295825856578	24.952555876025215	33.054696968273504	20.560451329844703
15	20.181133209947294	24.872229685929295	35.30476977856277	19.64186732556064
16	25.409146851307295	23.870031284937195	31.515816273804266	19.20500558995124
17	26.30635751261262	23.87261487584671	29.444480980073468	20.3765466314672
18	25.76521265301904	23.72488045020246	31.005439633224043	19.504467263554456
19	25.578489491831156	25.615364380267003	28.811266335340708	19.994879792561136
20	25.70579006210013	26.313168797737713	29.82661756278126	18.154423577380896
21	26.111883578696176	25.82698396294661	30.28085981905469	17.780272639302524
22	25.455651487678622	23.14615608647044	31.081538129104384	20.316654296746556
23	23.564462941911483	24.325917644516682	31.847455397826025	20.262164015745814
24	23.141693520353996	24.6361834255597	31.978279046607984	20.24384400747832
25	23.658176830356723	25.65623209101756	29.741828806568897	20.94376227205682
26	22.488514764047686	26.277468268806192	28.844618145263574	22.389398821882544
27	21.5964712845614	26.12761999605415	31.097274546462362	21.178634172922088
28	20.562800048853354	25.95451940511645	31.31312182335754	22.169558722672654
29	22.289343392113942	25.941836322469726	30.079574600013153	21.68924568540318
30	23.372337727003693	25.771554194342407	30.72664668689697	20.129461391756937
31	24.553038772653395	24.805760937984424	30.232711079377307	20.40848920998487
32	25.943245553874917	25.147734425644252	28.332597401377292	20.57642261910354
33	25.732330586897906	24.915915859490234	28.947961781644295	20.40379177196757
34	22.512941441737677	25.30274988021533	30.977724748921936	21.206583929125056
35	23.001944739339166	27.06428913670484	29.41700096767223	20.51676515628376
36	24.72449526028504	27.035869636700145	27.872953091383962	20.366682011630857
37	23.057609379844234	28.040651628601758	28.09983934761981	20.8018996439342
38	23.490948036940654	26.89377213667666	28.640044719609925	20.975235106772764
39	23.068648359184902	27.481421632641556	28.139062955064308	21.310867053109234
40	25.007515900827688	25.490647400907545	27.86027000873724	21.64156668952753
41	21.756888792852376	25.19799701242942	29.043084901494726	24.002029293223476
42	21.32448962335942	25.90331733072782	29.742533422271496	23.029659623641265
43	21.892175007750772	25.302515008314465	29.541952818932554	23.26335716500221
44	22.026521735045705	26.069841508441293	28.85918020311722	23.04445655339578
45	21.441455829990325	26.47570015313648	28.356084591463816	23.726759425409384
46	22.26162850781184	27.191119963172085	27.774541764921413	22.77270976409466
47	21.3611296398944	26.21311336796911	30.38702191824579	22.0387350738907
48	21.86845294576338	26.578104301913736	29.397976343702144	22.15546640862074
49	23.117501714564874	25.87748142163264	29.82544320327693	21.17957366052555
50	21.80245394162024	26.332898037410395	29.50695690570363	22.357691115265734
51	21.38203323907141	26.803816198645258	28.516502099754792	23.297648462528535
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	215.0
1	646.5
2	1078.0
3	4002.0
4	6926.0
5	4678.5
6	2431.0
7	2138.5
8	1846.0
9	1746.5
10	1647.0
11	1653.5
12	1660.0
13	1609.0
14	1558.0
15	1509.5
16	1461.0
17	1452.5
18	1444.0
19	1412.5
20	1381.0
21	1555.5
22	1730.0
23	2117.0
24	2504.0
25	3145.5
26	4330.0
27	4873.0
28	5832.0
29	6791.0
30	7805.5
31	8820.0
32	10070.0
33	11320.0
34	12782.5
35	14245.0
36	14962.5
37	15680.0
38	16974.5
39	18269.0
40	19870.0
41	21471.0
42	22410.0
43	23349.0
44	24281.5
45	25214.0
46	26031.0
47	26848.0
48	27865.0
49	28882.0
50	28321.5
51	27761.0
52	27408.5
53	27056.0
54	26292.5
55	25529.0
56	24707.5
57	23886.0
58	22729.5
59	21573.0
60	19828.5
61	18084.0
62	16486.5
63	14889.0
64	13021.5
65	11154.0
66	9443.5
67	7733.0
68	6278.5
69	4824.0
70	3993.5
71	3163.0
72	2630.0
73	2097.0
74	1649.0
75	920.0
76	639.0
77	495.5
78	352.0
79	263.5
80	175.0
81	138.5
82	102.0
83	76.0
84	50.0
85	39.5
86	29.0
87	21.5
88	14.0
89	12.5
90	11.0
91	10.0
92	9.0
93	5.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	425764.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.45662307969428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.41576230685315	29.0470223297202
2	9.516123273102934	8.080449179720759
3	5.107368024443352	6.505247974292228
4	3.5288999988124727	5.99300708542059
5	2.6753523228598577	5.679321258852276
6	2.2817695424377527	5.812573765080369
7	1.8073670037868195	5.371428975251737
8	1.4780845437765195	5.020358268403326
9	1.2385802696476573	4.732734209613902
>10	3.933678174689094	21.947091352018415
>50	0.007940118475538108	0.2509066166083181
>100	0.00737296715585664	0.5191503506790066
>500	5.6715131968128E-4	0.12376775521753682
>1k	0.00113430263936256	0.9169408791213622
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2406	0.565101793481835	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1402	0.32929040501310586	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	514	0.12072415704474779	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010569235538937063	0.0
2	0.0	0.0	0.0	0.05824823141458649	0.0
3	0.0	0.0	0.0	0.07703798348380793	0.0
4	0.0	0.0	0.0	0.11203389671273288	0.0
5	2.348719008652681E-4	0.0	0.0	0.25107806202497157	0.0
6	2.348719008652681E-4	0.0	0.0	0.3511334917935758	0.0
7	2.348719008652681E-4	0.0	0.0	0.44837045875179676	0.0
8	2.348719008652681E-4	0.0	0.0	0.6745520992850499	0.0
9	2.348719008652681E-4	0.0	0.0	0.7861162521960523	0.0
10	2.348719008652681E-4	0.0	0.0	1.143356413412125	0.0
11	2.348719008652681E-4	0.0	0.0	1.3841001117990248	0.0
12	2.348719008652681E-4	0.0	0.0	1.6617186986217718	0.0
13	2.348719008652681E-4	0.0	0.0	1.7446284796272113	0.0
14	2.348719008652681E-4	0.0	0.0	1.7979444011236272	0.0
15	2.348719008652681E-4	0.0	0.0	1.871459306094456	0.0
16	2.348719008652681E-4	0.0	0.0	1.9832583309063236	0.0
17	2.348719008652681E-4	0.0	0.0	2.1194840334081793	0.0
18	2.348719008652681E-4	0.0	0.0	2.2784923102939656	0.0
19	2.348719008652681E-4	0.0	0.0	2.386533384691989	0.0
20	2.348719008652681E-4	0.0	0.0	2.4980975376029915	0.0
21	2.348719008652681E-4	0.0	0.0	2.6418391409325355	0.0
22	2.348719008652681E-4	0.0	0.0	2.798498698809669	0.0
23	2.348719008652681E-4	0.0	0.0	2.9659623641266055	0.0
24	2.348719008652681E-4	0.0	0.0	3.0998393476198083	0.0
25	2.348719008652681E-4	0.0	0.0	3.2351255625182027	0.0
26	2.348719008652681E-4	0.0	0.0	3.36876767411054	0.0
27	2.348719008652681E-4	0.0	0.0	3.5348221080222846	0.0
28	2.348719008652681E-4	0.0	0.0	3.676919608045772	0.0
29	2.348719008652681E-4	0.0	0.0	3.8490807113800134	0.0
30	2.348719008652681E-4	0.0	0.0	4.094052103982488	0.0
31	2.348719008652681E-4	0.0	0.0	4.263159872605481	0.0
32	2.348719008652681E-4	0.0	0.0	4.444480980073468	0.0
33	2.348719008652681E-4	0.0	0.0	4.618755930515497	0.0
34	4.697438017305362E-4	0.0	0.0	4.813464736332804	0.0
35	4.697438017305362E-4	0.0	0.0	5.03306996364183	0.0
36	4.697438017305362E-4	0.0	0.0	5.217444405821065	0.0
37	4.697438017305362E-4	0.0	0.0	5.441277327345666	0.0
38	4.697438017305362E-4	0.0	0.0	5.6975225711896735	0.0
39	4.697438017305362E-4	0.0	0.0	6.143309439031952	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAAG	30	2.162269E-6	45.000004	2
CTCACGA	20	7.028279E-4	45.0	24
GTCGATA	20	7.028279E-4	45.0	1
TCGATAG	25	3.8866543E-5	45.0	2
GCGATAT	25	3.8866543E-5	45.0	9
TATCGTG	20	7.028279E-4	45.0	1
GCGTATG	25	3.8866543E-5	45.0	1
CGTTATT	200	0.0	43.875	1
CGTTTTA	585	0.0	43.846157	1
CGATAAG	45	1.9233994E-8	40.0	2
CGTTTTT	935	0.0	39.94652	1
AGGCGAT	255	0.0	39.705883	7
CATACGA	250	0.0	39.6	18
ACATACG	250	0.0	39.6	17
CGAATAT	40	3.451387E-7	39.375	14
CGAAAAG	35	6.2390245E-6	38.57143	2
ACGGGTA	35	6.2390245E-6	38.57143	5
CGTATGG	60	1.546141E-10	37.500004	2
ATAGGCC	30	1.1387148E-4	37.500004	5
CGCATAG	30	1.1387148E-4	37.500004	2
>>END_MODULE