##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934678.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	85915
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.905045684688353	31.0	31.0	34.0	30.0	34.0
2	32.09664202991328	33.0	31.0	34.0	30.0	34.0
3	31.750648897165803	33.0	31.0	34.0	30.0	34.0
4	35.63425478670779	37.0	35.0	37.0	33.0	37.0
5	31.98167956701391	37.0	35.0	37.0	0.0	37.0
6	33.923342838852356	37.0	35.0	37.0	19.0	37.0
7	20.915125414654018	32.0	0.0	37.0	0.0	37.0
8	28.219007158237794	35.0	17.0	37.0	17.0	37.0
9	34.9620089623465	37.0	32.0	39.0	32.0	39.0
10	36.360344526566955	37.0	35.0	39.0	32.0	39.0
11	36.72057265902345	39.0	35.0	39.0	33.0	39.0
12	36.90385846476168	39.0	35.0	39.0	33.0	39.0
13	36.39030437059885	39.0	35.0	39.0	32.0	39.0
14	37.72113135075365	40.0	36.0	41.0	33.0	41.0
15	38.03085607868242	40.0	36.0	41.0	33.0	41.0
16	38.1646511086539	40.0	36.0	41.0	34.0	41.0
17	38.2113600651807	40.0	36.0	41.0	34.0	41.0
18	38.086422627015075	40.0	36.0	41.0	34.0	41.0
19	37.96254437525461	40.0	36.0	41.0	34.0	41.0
20	37.893569225397194	40.0	35.0	41.0	34.0	41.0
21	37.75977419542571	39.0	35.0	41.0	34.0	41.0
22	37.67226910318338	39.0	35.0	41.0	33.0	41.0
23	37.61594599313275	39.0	35.0	41.0	33.0	41.0
24	37.502822557178604	39.0	35.0	41.0	33.0	41.0
25	37.338229645579936	39.0	35.0	41.0	33.0	41.0
26	37.20975382645638	39.0	35.0	41.0	33.0	41.0
27	37.140999825408834	39.0	35.0	41.0	33.0	41.0
28	37.118431007391024	39.0	35.0	41.0	33.0	41.0
29	37.153686783448755	39.0	35.0	41.0	33.0	41.0
30	36.99075830762963	39.0	35.0	41.0	33.0	41.0
31	36.80437641855322	39.0	35.0	40.0	32.0	41.0
32	36.62968049816679	39.0	35.0	40.0	31.0	41.0
33	36.382552522842346	39.0	35.0	40.0	31.0	41.0
34	36.16702554850725	39.0	35.0	40.0	30.0	41.0
35	35.90868882034569	38.0	35.0	40.0	30.0	41.0
36	35.66857940988186	38.0	35.0	40.0	28.0	41.0
37	35.51321655124251	38.0	35.0	40.0	27.0	41.0
38	35.395611942035735	38.0	35.0	40.0	26.0	41.0
39	35.35722516440668	38.0	35.0	40.0	26.0	41.0
40	35.227934586509924	38.0	35.0	40.0	25.0	41.0
41	35.1437350870046	38.0	35.0	40.0	24.0	41.0
42	35.031950183320724	38.0	34.0	40.0	24.0	41.0
43	34.910842111389165	38.0	34.0	40.0	23.0	41.0
44	34.72469301053367	38.0	34.0	40.0	23.0	41.0
45	34.62489670022697	38.0	34.0	40.0	23.0	41.0
46	34.49560612233021	38.0	34.0	40.0	23.0	41.0
47	34.46351626607694	38.0	34.0	40.0	23.0	41.0
48	34.425327358435666	37.0	34.0	40.0	23.0	41.0
49	34.359774195425715	37.0	34.0	40.0	22.0	41.0
50	34.13545946575103	37.0	34.0	40.0	20.0	41.0
51	32.42802770179829	35.0	31.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	5.0
11	5.0
12	1.0
13	0.0
14	0.0
15	1.0
16	10.0
17	12.0
18	24.0
19	46.0
20	99.0
21	191.0
22	304.0
23	497.0
24	757.0
25	1175.0
26	1480.0
27	1506.0
28	1526.0
29	1647.0
30	1864.0
31	2366.0
32	3215.0
33	5181.0
34	7154.0
35	9041.0
36	8686.0
37	14487.0
38	15307.0
39	9319.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.50648897165803	17.801315253448173	23.277658150497587	19.414537624396207
2	35.02182389571088	23.836349880696037	24.244893208403656	16.89693301518943
3	30.107664552173656	22.587441075481582	30.974800675085838	16.330093697258917
4	25.57993365535704	24.96071698772042	31.105162078798816	18.354187278123728
5	20.925333178141187	36.143863120526106	27.047663388232557	15.883140313100158
6	25.07361927486469	33.47843799103765	29.004248385031715	12.443694349065936
7	46.575103299773026	42.87493452831287	8.17319443636152	2.376767735552581
8	81.27102368620149	4.428795902927312	11.71972298201711	2.5804574288541002
9	76.47325845312227	6.483151952511203	13.101321073153699	3.9422685212128266
10	46.75667811208753	21.61438631205261	20.08380375952977	11.545131816330095
11	37.51964150613979	22.37676773555258	24.876913228190652	15.226677530116975
12	32.627597043589596	21.479369143921318	30.23220625036373	15.660827562125357
13	22.535063725775476	30.57789675842402	31.49391840772857	15.39312110807193
14	17.34272245824361	33.66932433218879	32.13292207414305	16.855031135424547
15	17.53593668160391	24.219286504102893	42.5688180178083	15.675958796484899
16	18.393761275679452	20.386428446720593	43.300936972589184	17.918873305010766
17	20.186230576732818	20.6494791363557	32.881336204388056	26.282954082523425
18	21.857650002909853	22.48967002269685	35.30000581970552	20.352674154687772
19	24.598731304196008	25.1201769190479	30.627946225920972	19.653145550835127
20	26.37141360647151	23.382412849909795	31.673165337833904	18.573008205784788
21	22.100913693767097	25.946575103299775	32.823139149159054	19.12937205377408
22	21.9717162311587	23.018099284176223	32.008380375952974	23.001804108712097
23	20.067508584065646	26.640284001629517	31.547459698539253	21.744747715765584
24	20.878775533957985	23.179887097712857	36.96793342256882	18.973403945760346
25	18.227317697724494	25.34481755223186	35.982075306989465	20.44578944305418
26	17.8758074841413	29.78641680730955	31.6440668102194	20.693708898329742
27	17.825758016644357	29.837630215911076	33.45865099225979	18.877960775184775
28	17.40091951347262	27.33050107664552	37.1739509980795	18.094628411802365
29	18.05854623756038	24.658092300529592	37.06686841645813	20.216493045451898
30	19.12937205377408	28.258162136995868	34.658674271081885	17.95379153814817
31	24.432287726241054	26.69498923354478	30.890996915556073	17.981726124658092
32	25.270325321538728	25.989640924169237	31.109817843217137	17.630215911074902
33	23.160100098934993	27.587732060757723	29.741023104230923	19.511144736076353
34	20.513298027119827	27.790257812954668	30.013385322702675	21.683058837222838
35	20.12221381598091	27.119827736716523	31.6149682826049	21.142990164697668
36	26.003608217424194	25.441424663912006	30.66286445905837	17.892102659605424
37	21.750567421288483	27.63079788162719	31.811674329278937	18.806960367805388
38	20.193214223360297	29.632776581504977	28.40016295175464	21.773846243380085
39	20.735610778094628	29.057789675842404	30.362567654076706	19.844031891986265
40	22.319734621428154	26.98946633300355	28.936739800966073	21.75405924460222
41	18.91637083163592	25.321538730140254	30.85142291800035	24.910667520223477
42	21.70633765931444	25.535703893382994	30.1169760810103	22.640982366292263
43	22.66775301169761	25.1318163300937	29.8888436245126	22.311587033696096
44	20.31077227492289	29.36623406855613	30.044811732526334	20.278181923994644
45	19.67526043182215	32.78589303381249	26.505266833498226	21.033579700867136
46	23.214805330850258	31.06209625792935	27.911307687830995	17.811790723389397
47	21.435139381947273	28.04166909154397	29.25915148693476	21.264040039574
48	22.98201710993424	26.099051387999765	30.3509282430309	20.56800325903509
49	22.03573299191061	25.290112320316595	31.20875283710644	21.465401850666357
50	20.23162427981144	29.441890240353843	29.2510038992027	21.07548158063202
51	19.98719664784962	29.353430716405747	27.917127393353898	22.742245242390734
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	107.0
1	186.5
2	266.0
3	1173.0
4	2080.0
5	1442.0
6	804.0
7	714.5
8	625.0
9	603.0
10	581.0
11	609.5
12	638.0
13	604.5
14	571.0
15	574.0
16	577.0
17	522.5
18	468.0
19	433.0
20	398.0
21	382.5
22	367.0
23	420.5
24	474.0
25	575.5
26	745.0
27	813.0
28	1029.5
29	1246.0
30	1361.0
31	1476.0
32	1634.0
33	1792.0
34	2165.5
35	2539.0
36	2795.5
37	3052.0
38	3328.5
39	3605.0
40	4426.5
41	5248.0
42	6047.5
43	6847.0
44	7106.0
45	7365.0
46	7084.0
47	6803.0
48	6620.0
49	6437.0
50	6315.0
51	6193.0
52	5804.0
53	5415.0
54	4972.0
55	4529.0
56	4043.0
57	3557.0
58	3217.0
59	2877.0
60	2636.5
61	2396.0
62	2157.0
63	1918.0
64	1535.5
65	1153.0
66	957.5
67	762.0
68	610.0
69	458.0
70	369.5
71	281.0
72	250.0
73	219.0
74	181.0
75	112.5
76	82.0
77	61.5
78	41.0
79	30.0
80	19.0
81	14.0
82	9.0
83	6.0
84	3.0
85	3.0
86	3.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	85915.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.88855263923646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.45236737022248	31.1761624861782
2	9.33205414095317	8.378048070767619
3	5.390758699372504	7.259500669266135
4	3.6742208162630297	6.597218180760053
5	2.784836384380024	6.250363731595181
6	2.1288181299590314	5.733573881161613
7	1.643935072343515	5.165570622126521
8	1.3431519991702536	4.823371937379969
9	1.0916351190167504	4.4101728452540305
>10	3.1452574806824667	18.406564627829834
>50	0.005185915054711404	0.20252575219693883
>100	0.005185915054711404	0.730955013676308
>500	0.002592957527355702	0.8659721818076005
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	744	0.8659721818076005	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	457	0.5319210847931095	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	171	0.1990339288831985	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	95	0.1105744049351103	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03491823313740325	0.0
2	0.0	0.0	0.0	0.13152534481755224	0.0
3	0.0	0.0	0.0	0.182738753419077	0.0
4	0.0	0.0	0.0	0.24209974975266252	0.0
5	0.0	0.0	0.0	0.4225106209625793	0.0
6	0.0	0.0	0.0	0.5423965547343305	0.0
7	0.0	0.0	0.0	0.6809055461793633	0.0
8	0.0	0.0	0.0	1.0498748763312575	0.0
9	0.0	0.0	0.0	1.1569574579526276	0.0
10	0.0	0.0	0.0	1.4782052028167374	0.0
11	0.0	0.0	0.0	1.9321422336029797	0.0
12	0.0	0.0	0.0	2.36163650119304	0.0
13	0.0	0.0	0.0	2.503637315951813	0.0
14	0.0	0.0	0.0	2.5664901355991385	0.0
15	0.0	0.0	0.0	2.68870395158005	0.0
16	0.0	0.0	0.0	2.9401152301693534	0.0
17	0.0	0.0	0.0	3.2625269161380435	0.0
18	0.0	0.0	0.0	3.5849386021067335	0.0
19	0.0	0.0	0.0	3.7595297677937496	0.0
20	0.0	0.0	0.0	3.957399755572368	0.0
21	0.0	0.0	0.0	4.209974975266252	0.0
22	0.0	0.0	0.0	4.456730489437234	0.0
23	0.0	0.0	0.0	4.745387883373101	0.0
24	0.0	0.0	0.0	4.971192457661642	0.0
25	0.0	0.0	0.0	5.172554268754001	0.0
26	0.0	0.0	0.0	5.365768492114299	0.0
27	0.0	0.0	0.0	5.547343304428796	0.0
28	0.0	0.0	0.0	5.768492114299017	0.0
29	0.0	0.0	0.0	5.980329395332596	0.0
30	0.0	0.0	0.0	6.23290461502648	0.0
31	0.0	0.0	0.0	6.506430774602805	0.0
32	0.0	0.0	0.0	6.708956526799744	0.0
33	0.0	0.0	0.0	6.921957748937904	0.0
34	0.0	0.0	0.0	7.144270499912705	0.0
35	0.0	0.0	0.0	7.435255776057732	0.0
36	0.0	0.0	0.0	7.6726997613920735	0.0
37	0.0	0.0	0.0	7.926438922190537	0.0
38	0.0011639411045801082	0.0	0.0	8.183669906302741	0.0
39	0.0011639411045801082	0.0	0.0	8.453704242565326	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGCGAT	60	0.0	45.000004	7
TAGGAGA	30	2.1420474E-6	45.000004	6
CGTTTTA	115	0.0	45.000004	1
AAGGAGC	30	2.1420474E-6	45.000004	6
AGGTATT	25	3.86064E-5	45.0	7
GGGCTTA	20	6.996856E-4	45.0	8
GGCACTC	20	6.996856E-4	45.0	8
AGGACTC	20	6.996856E-4	45.0	7
AGGACTA	40	6.688424E-9	45.0	7
TGTATGG	25	3.86064E-5	45.0	2
GATCAAT	20	6.996856E-4	45.0	9
CGATTGG	20	6.996856E-4	45.0	10
CGTTATT	45	3.765308E-10	45.0	1
TAGTTGG	20	6.996856E-4	45.0	2
CGTTAGG	25	3.86064E-5	45.0	3
AGGCCTT	20	6.996856E-4	45.0	7
CCTCCGC	20	6.996856E-4	45.0	45
TAATTGT	25	3.86064E-5	45.0	36
CACTAGG	20	6.996856E-4	45.0	3
TTGCGGG	20	6.996856E-4	45.0	3
>>END_MODULE