##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934677.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	456413
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.918534309934206	31.0	31.0	34.0	30.0	34.0
2	32.10282134820875	33.0	31.0	34.0	30.0	34.0
3	31.92963390613326	33.0	31.0	34.0	30.0	34.0
4	35.721677515758756	37.0	35.0	37.0	35.0	37.0
5	32.087414249813214	37.0	35.0	37.0	10.0	37.0
6	33.92514674209543	37.0	35.0	37.0	19.0	37.0
7	20.763939677441265	32.0	0.0	37.0	0.0	37.0
8	28.05719600449593	35.0	17.0	37.0	17.0	37.0
9	34.784377307394834	37.0	32.0	39.0	32.0	39.0
10	36.208160153194584	37.0	35.0	39.0	32.0	39.0
11	36.59725511762373	38.0	35.0	39.0	32.0	39.0
12	36.89562961615905	39.0	35.0	39.0	33.0	39.0
13	36.62506983806333	39.0	35.0	39.0	33.0	39.0
14	37.86344823657521	40.0	37.0	41.0	33.0	41.0
15	38.039376617230445	40.0	37.0	41.0	33.0	41.0
16	38.16517058015438	40.0	37.0	41.0	33.0	41.0
17	38.15513580901508	40.0	37.0	41.0	33.0	41.0
18	38.04942234336007	40.0	37.0	41.0	33.0	41.0
19	37.98178185108662	40.0	37.0	41.0	33.0	41.0
20	37.94476274777449	40.0	36.0	41.0	33.0	41.0
21	37.83735783161303	40.0	36.0	41.0	33.0	41.0
22	37.84609334089958	40.0	36.0	41.0	33.0	41.0
23	37.808923058720936	40.0	36.0	41.0	33.0	41.0
24	37.66665279034559	39.0	35.0	41.0	33.0	41.0
25	37.524792238608455	39.0	35.0	41.0	33.0	41.0
26	37.388722494758035	39.0	35.0	41.0	33.0	41.0
27	37.34974463917548	39.0	35.0	41.0	33.0	41.0
28	37.25004765420792	39.0	35.0	41.0	32.0	41.0
29	37.209455033051206	39.0	35.0	41.0	32.0	41.0
30	37.00663215114381	39.0	35.0	41.0	32.0	41.0
31	36.84620069980478	39.0	35.0	41.0	31.0	41.0
32	36.738114383245	39.0	35.0	41.0	31.0	41.0
33	36.554158185678325	39.0	35.0	40.0	30.0	41.0
34	36.32338693244934	39.0	35.0	40.0	30.0	41.0
35	36.158796966782276	39.0	35.0	40.0	29.0	41.0
36	35.96904777033082	39.0	35.0	40.0	29.0	41.0
37	35.919702111903035	39.0	35.0	40.0	29.0	41.0
38	35.691961008998426	38.0	35.0	40.0	27.0	41.0
39	35.57712203640124	38.0	35.0	40.0	27.0	41.0
40	35.40560194385348	38.0	34.0	40.0	26.0	41.0
41	35.24673924712924	38.0	34.0	40.0	26.0	41.0
42	35.19010194713998	38.0	34.0	40.0	26.0	41.0
43	35.140729996735416	38.0	34.0	40.0	25.0	41.0
44	34.99735108333899	38.0	34.0	40.0	25.0	41.0
45	34.89860499153179	38.0	34.0	40.0	24.0	41.0
46	34.73433491158227	38.0	34.0	40.0	24.0	41.0
47	34.64788031892168	37.0	34.0	40.0	24.0	41.0
48	34.49844329587457	37.0	33.0	40.0	23.0	41.0
49	34.38849024896311	37.0	33.0	40.0	23.0	41.0
50	34.207198305043896	37.0	33.0	40.0	23.0	41.0
51	32.25294415365031	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	10.0
9	20.0
10	20.0
11	18.0
12	9.0
13	11.0
14	17.0
15	27.0
16	56.0
17	117.0
18	199.0
19	341.0
20	627.0
21	1028.0
22	1686.0
23	2506.0
24	3603.0
25	5002.0
26	6116.0
27	7084.0
28	7919.0
29	8826.0
30	10817.0
31	13540.0
32	18170.0
33	25972.0
34	34479.0
35	42728.0
36	54006.0
37	83562.0
38	79416.0
39	48471.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.16488136840975	18.168851456904164	22.015367660430357	17.65089951425573
2	32.520984283970876	24.474105689364674	25.679154625306467	17.32575540135798
3	32.21292995598285	24.24974748747297	27.337301961162368	16.200020595381815
4	27.95056231965347	25.253662801015746	28.350419466579613	18.445355412751173
5	22.75548680690515	36.39642166195967	24.0356869764884	16.81240455464678
6	26.108152046501743	34.294378118064124	26.194039170663412	13.403430664770724
7	49.23829952258152	42.57240700856461	5.8039538751087285	2.3853395937451385
8	84.57690731859083	4.37717593495365	8.289641180246837	2.756275566208675
9	79.32377035711077	5.8330941493778665	10.00037246967111	4.8427630238402495
10	44.42314307436466	24.83693496898642	17.385788748348535	13.354133208300379
11	33.18880049428916	25.252129102370002	23.93709206354771	17.621978339793127
12	29.65340601604249	22.1023502836247	28.693748863419756	19.55049483691306
13	25.93287220127385	23.61874004465254	30.75788814078477	19.690499613288843
14	22.47569635396012	24.566127608109323	31.102532136464124	21.855643901466433
15	20.482326314105865	25.490509691879943	33.30645709039839	20.720706903615806
16	26.089528562946278	24.31985942556413	29.713439363033046	19.87717264845655
17	26.395610992675493	23.877277816363687	27.94727582255545	21.77983536840537
18	26.093910559076978	23.739902237666325	29.192639122899656	20.973548080357045
19	26.252757918814755	25.542217246222172	25.952591183861983	22.252433651101086
20	27.468980944889825	26.524660778724535	27.032095930659295	18.97426234572635
21	26.23654453313118	26.11121944379323	28.867056810388835	18.785179212686757
22	26.437020856110582	22.411719210451935	29.174892038570334	21.97636789486715
23	25.994439246910144	24.389971363655285	28.769776496287353	20.845812893147215
24	24.213376919588182	23.416072723607783	30.10277971924551	22.267770637558527
25	24.127270695619977	25.054939276488618	27.706923334786694	23.110866693104708
26	23.39306724392162	24.914934500112835	27.98014079353568	23.71185746242986
27	22.716925240955014	24.64566083788148	29.78026480402618	22.85714911713733
28	20.926222522145512	26.77859745449845	28.35392506348417	23.94125495987187
29	24.273848466191804	25.67784002646726	27.518497501166706	22.52981400617423
30	25.308875952262532	24.883384127971812	28.306161305659565	21.501578614106084
31	25.413605659786203	25.01506311169927	27.748333198221786	21.82299803029274
32	26.917944931454624	24.0074231014454	27.487823528251827	21.58680843884815
33	25.55251493712931	24.282831558259733	28.65672099611536	21.507932508495596
34	22.902940976703118	24.49820666808351	30.95880266337725	21.640049691836122
35	23.765098715417835	26.13225302522058	29.13172937668296	20.970918882678628
36	25.011557514794713	27.478402236570826	27.014129746523434	20.495910502111027
37	24.549476022812673	26.989809667998067	27.825675429928598	20.63503887926067
38	24.47651578723656	26.48368911490251	27.624541807529585	21.415253290331346
39	24.770985927219428	25.497520885689056	27.288661804111626	22.44283138297989
40	26.292414983797567	24.12507969755463	28.640069410818707	20.942435907829093
41	21.624931805185216	25.073562760044084	28.839231134958908	24.462274299811792
42	22.12754676137621	24.976063346136065	29.40648053407769	23.489909358410035
43	22.7771776877521	24.699559390289057	29.259245464086252	23.264017457872583
44	23.089394912064293	24.54618952571465	29.04189845600366	23.322517106217393
45	23.010738081518273	24.538740132292464	28.101302986549463	24.3492187996398
46	23.75502012431723	25.657025544846444	27.032315030465824	23.555639300370498
47	22.087013297167257	25.963327074382192	28.88830949162272	23.06135013682783
48	21.940654626401965	25.868018658539526	29.093825110152427	23.097501604906082
49	23.43162880987176	25.020102407249574	28.478592853402514	23.069675929476155
50	21.891138070125084	25.91008582139422	28.941988944223763	23.256787164256934
51	21.67401016184903	25.505408478724313	28.01256756490284	24.80801379452382
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	222.0
1	633.5
2	1045.0
3	3948.5
4	6852.0
5	4500.5
6	2149.0
7	1866.5
8	1584.0
9	1517.5
10	1451.0
11	1437.5
12	1424.0
13	1424.0
14	1424.0
15	1385.5
16	1347.0
17	1326.5
18	1306.0
19	1327.0
20	1348.0
21	1447.5
22	1547.0
23	1804.5
24	2062.0
25	2480.0
26	3509.5
27	4121.0
28	5038.5
29	5956.0
30	6779.5
31	7603.0
32	8659.0
33	9715.0
34	11090.0
35	12465.0
36	13331.5
37	14198.0
38	15212.5
39	16227.0
40	18000.0
41	19773.0
42	20983.0
43	22193.0
44	23715.0
45	25237.0
46	28081.0
47	30925.0
48	31227.5
49	31530.0
50	32177.0
51	32824.0
52	32719.5
53	32615.0
54	31572.0
55	30529.0
56	29339.0
57	28149.0
58	26904.5
59	25660.0
60	23803.5
61	21947.0
62	19779.0
63	17611.0
64	15518.0
65	13425.0
66	11489.0
67	9553.0
68	7990.0
69	6427.0
70	5373.5
71	4320.0
72	3591.5
73	2863.0
74	2336.5
75	1430.5
76	1051.0
77	793.0
78	535.0
79	384.0
80	233.0
81	180.0
82	127.0
83	89.5
84	52.0
85	48.5
86	45.0
87	29.5
88	14.0
89	14.0
90	14.0
91	9.5
92	5.0
93	2.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	456413.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.906767451151964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.75991447796807	33.19118853281449
2	9.752225168472346	9.148907162176073
3	5.2520425149895935	7.390690106825404
4	3.479019312428153	6.527581993845359
5	2.5770412150580286	6.044033649338052
6	1.9651283376671855	5.53066907679741
7	1.4651788398675065	4.810876218120963
8	1.189061877691684	4.462003918553114
9	0.8809344116206661	3.718960702704717
>10	2.647176113379337	16.387355095876615
>50	0.01866056315196044	0.6103489936195808
>100	0.01159980952687294	0.9971399256213052
>500	0.001008679089293299	0.2711085064678566
>1k	0.001008679089293299	0.909136117239069
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2412	0.5284687333621084	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1431	0.31353182315140016	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	608	0.13321268237320147	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	538	0.11787569591576051	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1909980653487084E-4	0.0	0.0	0.02081448162081273	0.0
2	2.1909980653487084E-4	0.0	0.0	0.10998810288050516	0.0
3	2.1909980653487084E-4	0.0	0.0	0.15139796631559574	0.0
4	2.1909980653487084E-4	0.0	0.0	0.2329030943465677	0.0
5	2.1909980653487084E-4	0.0	0.0	0.5409574223345961	0.0
6	2.1909980653487084E-4	0.0	0.0	0.7613718277086762	0.0
7	2.1909980653487084E-4	0.0	0.0	0.9655728473991757	0.0
8	2.1909980653487084E-4	0.0	0.0	1.3985140651120804	0.0
9	2.1909980653487084E-4	0.0	0.0	1.6465350461095543	0.0
10	2.1909980653487084E-4	0.0	0.0	2.2302169307184503	0.0
11	2.1909980653487084E-4	0.0	0.0	2.6642536474640295	0.0
12	2.1909980653487084E-4	0.0	0.0	3.07046468877968	0.0
13	2.1909980653487084E-4	0.0	0.0	3.235008643487368	0.0
14	2.1909980653487084E-4	0.0	0.0	3.3355754546868734	0.0
15	2.1909980653487084E-4	0.0	0.0	3.461557843444424	0.0
16	2.1909980653487084E-4	0.0	0.0	3.656994870873529	0.0
17	2.1909980653487084E-4	0.0	0.0	3.865796986501261	0.0
18	2.1909980653487084E-4	0.0	0.0	4.115132566337944	0.0
19	2.1909980653487084E-4	0.0	0.0	4.263244035555517	0.0
20	2.1909980653487084E-4	0.0	0.0	4.429102589102413	0.0
21	2.1909980653487084E-4	0.0	0.0	4.629359812275286	0.0
22	2.1909980653487084E-4	0.0	0.0	4.8429821236467845	0.0
23	2.1909980653487084E-4	0.0	0.0	5.058576333277098	0.0
24	2.1909980653487084E-4	0.0	0.0	5.235828076763808	0.0
25	2.1909980653487084E-4	0.0	0.0	5.40322032895645	0.0
26	2.1909980653487084E-4	0.0	0.0	5.568859782696812	0.0
27	2.1909980653487084E-4	0.0	0.0	5.763420410899777	0.0
28	2.1909980653487084E-4	0.0	0.0	5.951627144713231	0.0
29	2.1909980653487084E-4	0.0	0.0	6.1492551702076845	0.0
30	2.1909980653487084E-4	0.0	0.0	6.453365701678085	0.0
31	2.1909980653487084E-4	0.0	0.0	6.671150909373747	0.0
32	2.1909980653487084E-4	0.0	0.0	6.966716548389288	0.0
33	2.1909980653487084E-4	0.0	0.0	7.188007352989508	0.0
34	2.1909980653487084E-4	0.0	0.0	7.413899253526959	0.0
35	2.1909980653487084E-4	0.0	0.0	7.667397729687805	0.0
36	2.1909980653487084E-4	0.0	0.0	7.883868338544257	0.0
37	2.1909980653487084E-4	0.0	0.0	8.126630924184894	0.0
38	2.1909980653487084E-4	0.0	0.0	8.406859576742994	0.0
39	2.1909980653487084E-4	0.0	0.0	8.84659288845848	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGTC	20	7.028811E-4	45.000004	5
TACGAAT	60	0.0	45.000004	12
CGAATAT	60	0.0	45.000004	14
CGCACGG	45	3.8380676E-10	45.000004	2
AATACCG	20	7.028811E-4	45.000004	1
CGTTTTA	515	0.0	42.37864	1
TTACGGG	75	0.0	42.0	3
CGTTATT	205	0.0	40.609756	1
CGTTTTT	850	0.0	40.5	1
ATTATAC	140	0.0	40.178574	42
TATGCGG	45	1.9239451E-8	40.000004	2
ATAGGCC	40	3.4521145E-7	39.375004	5
CGCATGG	40	3.4521145E-7	39.375004	2
ATAGCGG	40	3.4521145E-7	39.375004	2
TATACGG	35	6.240012E-6	38.57143	2
TCGTTGA	100	0.0	38.25	24
TTAATCG	100	0.0	38.25	20
CGTTGAT	100	0.0	38.25	25
ATAGGCG	30	1.13884365E-4	37.500004	5
ACGTTAG	30	1.13884365E-4	37.500004	2
>>END_MODULE