##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934675.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	341268
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.936416540665988	31.0	31.0	34.0	30.0	34.0
2	32.12480513848354	33.0	31.0	34.0	30.0	34.0
3	31.962618821571315	33.0	31.0	34.0	30.0	34.0
4	35.76004196115663	37.0	35.0	37.0	35.0	37.0
5	32.0483901215467	37.0	35.0	37.0	0.0	37.0
6	33.95389254193186	37.0	35.0	37.0	19.0	37.0
7	20.860376595520236	32.0	0.0	37.0	0.0	37.0
8	28.172864141964673	35.0	17.0	37.0	17.0	37.0
9	34.91242073678165	37.0	32.0	39.0	32.0	39.0
10	36.15114221081379	37.0	35.0	39.0	32.0	39.0
11	36.57902879848096	38.0	35.0	39.0	32.0	39.0
12	36.97187547616536	39.0	37.0	39.0	33.0	39.0
13	36.76688116084719	39.0	37.0	39.0	33.0	39.0
14	38.17179167106204	40.0	37.0	41.0	33.0	41.0
15	38.3502350059191	40.0	38.0	41.0	34.0	41.0
16	38.41105524104223	40.0	38.0	41.0	34.0	41.0
17	38.36580634574587	40.0	37.0	41.0	34.0	41.0
18	38.29180292321577	40.0	37.0	41.0	34.0	41.0
19	38.22352520599646	40.0	37.0	41.0	34.0	41.0
20	38.20355849361792	40.0	37.0	41.0	34.0	41.0
21	38.12762403741341	40.0	37.0	41.0	34.0	41.0
22	38.10621271258952	40.0	37.0	41.0	34.0	41.0
23	38.04538661696965	40.0	37.0	41.0	34.0	41.0
24	37.92403917156018	40.0	36.0	41.0	33.0	41.0
25	37.806747189891816	40.0	36.0	41.0	33.0	41.0
26	37.66541252036522	40.0	36.0	41.0	33.0	41.0
27	37.627465804001545	40.0	36.0	41.0	33.0	41.0
28	37.569898144566736	40.0	36.0	41.0	33.0	41.0
29	37.56355415685034	40.0	36.0	41.0	33.0	41.0
30	37.399958390473174	40.0	36.0	41.0	33.0	41.0
31	37.26325058311942	39.0	35.0	41.0	32.0	41.0
32	37.15080816249986	39.0	35.0	41.0	32.0	41.0
33	36.97951463366035	39.0	35.0	41.0	31.0	41.0
34	36.837555821231405	39.0	35.0	41.0	31.0	41.0
35	36.661497708545774	39.0	35.0	41.0	30.0	41.0
36	36.49477829740849	39.0	35.0	41.0	30.0	41.0
37	36.40418380861962	39.0	35.0	41.0	30.0	41.0
38	36.2982992838473	39.0	35.0	41.0	30.0	41.0
39	36.25101972643201	39.0	35.0	41.0	30.0	41.0
40	36.17810342604639	39.0	35.0	41.0	29.0	41.0
41	36.076080968622904	39.0	35.0	40.0	29.0	41.0
42	35.932780102441484	39.0	35.0	40.0	28.0	41.0
43	35.79722095244793	39.0	35.0	40.0	27.0	41.0
44	35.649032431988935	39.0	35.0	40.0	27.0	41.0
45	35.52173951264109	38.0	35.0	40.0	26.0	41.0
46	35.36516462135331	38.0	34.0	40.0	26.0	41.0
47	35.311122050705016	38.0	34.0	40.0	26.0	41.0
48	35.22035174701408	38.0	34.0	40.0	25.0	41.0
49	35.078422237068814	38.0	34.0	40.0	24.0	41.0
50	34.92520248016222	38.0	34.0	40.0	24.0	41.0
51	33.05888041070361	36.0	31.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	11.0
9	12.0
10	16.0
11	12.0
12	2.0
13	4.0
14	4.0
15	12.0
16	23.0
17	45.0
18	82.0
19	171.0
20	329.0
21	532.0
22	995.0
23	1582.0
24	2382.0
25	3113.0
26	4075.0
27	4444.0
28	5054.0
29	5691.0
30	6905.0
31	8936.0
32	11736.0
33	17706.0
34	24083.0
35	30440.0
36	37797.0
37	64503.0
38	67074.0
39	43489.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.75261671179249	18.4576344690976	21.870787767971215	16.918961051138695
2	31.79583201472157	23.95448738235053	26.14279686346215	18.106883739465758
3	32.25236471043286	23.227199737449748	27.872815499841767	16.64762005227563
4	27.503897230329244	25.898707174420103	27.81186633379045	18.7855292614602
5	21.970123187641384	38.21278291547991	23.71918843841204	16.09790545846666
6	26.296927927611147	36.0186129376326	25.44129540419846	12.243163730557802
7	50.13010302753261	42.27879555071088	5.435317697528042	2.1557837242284656
8	85.72910439888885	3.889318658649507	7.869475016702415	2.512101925759227
9	80.18214423854566	6.04041398548941	9.637000832190537	4.1404409437743945
10	39.9770854577634	27.609386171571902	18.986251274658038	13.427277096006657
11	30.030943422764512	25.44979312446523	27.211751468054434	17.307511984715823
12	27.428882872112244	23.470703376818218	30.358252165453543	18.742161585615996
13	23.08684084062965	26.461314861047626	31.366550628831302	19.085293669491428
14	19.744892577094834	27.94109028681271	31.60976124336299	20.704255892729467
15	20.19439267672328	26.403588968200946	35.20546901555376	18.196549339522015
16	22.758066973756698	24.755910310957958	32.93394048079515	19.552082234490197
17	22.208352379947723	25.169368355661824	30.700798199655406	21.921481064735048
18	22.80876027052053	24.482518138237396	32.61337130935218	20.095350281889893
19	23.246539376677568	27.129997538591372	29.098831416950897	20.52463166778016
20	24.414243351266453	26.09415474055581	30.682630659774723	18.808971248403015
21	23.719481463248826	26.846056471746543	30.669151517282607	18.765310547722024
22	23.02764982359905	24.57013256443616	30.46139690800192	21.940820703962867
23	21.762368578360704	26.363151540724594	30.74211470164211	21.132365179272593
24	21.069364839363786	24.59679782458361	33.91586670886224	20.41797062719036
25	20.677590632582017	25.984270426761373	31.990107481510133	21.348031459146476
26	20.374016901672586	27.942555410996633	30.999097483502702	20.68433020382808
27	20.340026020605507	28.20422659024579	30.937269242941028	20.518478146207674
28	19.44864446710503	27.5285113166192	32.07127536129962	20.951568854976145
29	20.350281889892987	26.308062871408982	31.55203535051631	21.789619888181722
30	20.927540818359763	27.12443006669245	31.57811456099019	20.369914553957592
31	22.85535121956937	26.320955964227526	29.82318881348383	21.00050400271927
32	22.5031353657536	26.658520576204037	29.787146758559256	21.051197299483103
33	21.94785330004571	26.735586108278536	29.23450191638243	22.082058675293318
34	20.180620509394377	27.227574809240828	30.285875030767606	22.305929650597182
35	21.23082152443241	26.541603666326758	30.62197451856019	21.605600290680638
36	22.347832202257464	27.651581748068963	29.372809639345032	20.62777641032854
37	20.978820164797167	27.88336439396603	30.46344808185942	20.67436735937738
38	20.40449148469824	28.791155338326472	28.79936003375646	22.00499314321882
39	20.534594512230857	28.101374872534198	29.579392149278572	21.784638465956373
40	21.92382526342933	25.506932967638335	29.867142539001605	22.702099229930727
41	19.315611191204564	25.73109696777899	30.26536329219265	24.6879285488238
42	20.55686439982653	25.577551953303562	29.390684154388925	24.474899492480983
43	21.126797707373676	26.140452664767867	29.266148598755233	23.466601029103227
44	20.119085293669492	27.029783044410845	29.25413458044704	23.596997081472626
45	20.632171782880317	28.007606924762946	28.39293458513544	22.967286707221305
46	21.058815945239516	27.944899609690914	28.489925806111327	22.506358638958236
47	19.671636367898543	27.019820199960147	30.298768123586157	23.009775308555152
48	20.582650585463625	26.18206219159136	30.679407386570084	22.55587983637493
49	20.70718614109732	25.947349297326443	30.294372751034377	23.051091810541863
50	19.58343589202621	26.756390871690282	30.259502795456942	23.400670440826566
51	19.60511972994831	26.915796382901412	29.257650878488462	24.221433008661812
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	165.0
1	495.0
2	825.0
3	2660.5
4	4496.0
5	3058.0
6	1620.0
7	1419.0
8	1218.0
9	1172.0
10	1126.0
11	1154.5
12	1183.0
13	1167.5
14	1152.0
15	1134.0
16	1116.0
17	1142.5
18	1169.0
19	1314.0
20	1459.0
21	1529.0
22	1599.0
23	1910.5
24	2222.0
25	2488.0
26	3556.0
27	4358.0
28	5150.5
29	5943.0
30	6687.5
31	7432.0
32	8780.5
33	10129.0
34	11081.5
35	12034.0
36	13026.0
37	14018.0
38	15226.5
39	16435.0
40	18286.5
41	20138.0
42	21781.0
43	23424.0
44	24586.5
45	25749.0
46	26262.0
47	26775.0
48	26607.0
49	26439.0
50	25318.0
51	24197.0
52	22348.0
53	20499.0
54	19034.5
55	17570.0
56	16347.0
57	15124.0
58	14012.0
59	12900.0
60	12077.0
61	11254.0
62	9951.0
63	8648.0
64	7331.0
65	6014.0
66	4980.5
67	3947.0
68	3161.0
69	2375.0
70	1885.0
71	1395.0
72	1213.0
73	1031.0
74	846.5
75	513.0
76	364.0
77	263.5
78	163.0
79	114.5
80	66.0
81	51.5
82	37.0
83	27.5
84	18.0
85	13.0
86	8.0
87	9.0
88	10.0
89	5.5
90	1.0
91	3.0
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	341268.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.77275704261516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.99242816766488	30.365418818808386
2	8.620181009296857	7.37417815948039
3	4.422371912258369	5.674711180653379
4	3.121268114824104	5.340209709609313
5	2.397258013410226	5.126866728802894
6	1.954511254525565	5.015990101612737
7	1.6033513682762457	4.8005830970457115
8	1.3254314624294388	4.535388633530599
9	1.0605744829255535	4.08273252153947
>10	4.482514691120758	26.335577836136494
>50	0.016642364083836736	0.44401007248846747
>100	0.002080295510479592	0.19190014488980525
>500	6.934318368265306E-4	0.25655892632099153
>1k	6.934318368265306E-4	0.4558740690813457
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1537	0.45037917413879996	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	865	0.25346648381916853	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	342	0.10021449418052675	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03164668237279792	0.0
2	0.0	0.0	0.0	0.15149384061793078	0.0
3	0.0	0.0	0.0	0.20013596352426832	0.0
4	0.0	0.0	0.0	0.2637223531066493	0.0
5	0.0	0.0	0.0	0.4623931924469918	0.0
6	0.0	0.0	0.0	0.6314685232720325	0.0
7	0.0	0.0	0.0	0.7970275560556512	0.0
8	0.0	0.0	0.0	1.2233786935780677	0.0
9	0.0	0.0	0.0	1.3552398701313924	0.0
10	0.0	0.0	0.0	1.8246656586612282	0.0
11	0.0	0.0	0.0	2.380533774042688	0.0
12	0.0	0.0	0.0	2.835308320733265	0.0
13	0.0	0.0	0.0	2.997351055475462	0.0
14	0.0	0.0	0.0	3.0732444882028203	0.0
15	0.0	0.0	0.0	3.228254626862173	0.0
16	0.0	0.0	0.0	3.576075108126165	0.0
17	0.0	0.0	0.0	4.001547171138226	0.0
18	0.0	0.0	0.0	4.499103343999438	0.0
19	0.0	0.0	0.0	4.784509535028189	0.0
20	0.0	0.0	0.0	5.0687436267098	0.0
21	0.0	0.0	0.0	5.4578806099605	0.0
22	0.0	0.0	0.0	5.8549292638044	0.0
23	0.0	0.0	0.0	6.312341034026044	0.0
24	0.0	0.0	0.0	6.65019867083934	0.0
25	0.0	0.0	0.0	6.968130618751245	0.0
26	0.0	0.0	0.0	7.256467058147849	0.0
27	0.0	0.0	0.0	7.554180292321577	0.0
28	0.0	0.0	0.0	7.8501353774745946	0.0
29	0.0	0.0	0.0	8.146969537137968	0.0
30	0.0	0.0	0.0	8.492738844544464	0.0
31	0.0	0.0	0.0	8.827080183316339	0.0
32	0.0	0.0	0.0	9.171384366538907	0.0
33	0.0	0.0	0.0	9.514809475251122	0.0
34	0.0	0.0	0.0	9.852667112064418	0.0
35	0.0	0.0	0.0	10.20839926392161	0.0
36	0.0	0.0	0.0	10.564131415778801	0.0
37	0.0	0.0	0.0	10.937445057843103	0.0
38	0.0	0.0	0.0	11.306363327355626	0.0
39	0.0	0.0	0.0	11.644513989005707	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTT	30	2.1609994E-6	45.000004	27
TCGACGT	30	2.1609994E-6	45.000004	26
TTCGACG	30	2.1609994E-6	45.000004	25
CGACGGT	20	7.026309E-4	45.0	28
ACGTTCC	20	7.026309E-4	45.0	17
CACGACG	20	7.026309E-4	45.0	26
CGTAGCC	20	7.026309E-4	45.0	16
CTTGCGA	20	7.026309E-4	45.0	1
ATAAGGT	40	6.7902874E-9	45.0	4
GTCTTCG	20	7.026309E-4	45.0	32
CACCGGA	20	7.026309E-4	45.0	31
ACGACGG	20	7.026309E-4	45.0	27
CACGCCG	50	2.1827873E-11	44.999996	26
GCGATAT	50	2.1827873E-11	44.999996	9
CATCGTT	25	3.8850234E-5	44.999996	36
CGATATC	25	3.8850234E-5	44.999996	10
TGGTCGG	25	3.8850234E-5	44.999996	45
CGAAGGA	90	0.0	42.500004	4
GCGAGAC	80	0.0	42.1875	21
ACACGAC	80	0.0	42.1875	26
>>END_MODULE