##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934672.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	274053
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0525664743681	33.0	31.0	34.0	30.0	34.0
2	32.24370468486023	33.0	31.0	34.0	30.0	34.0
3	32.1293253494762	33.0	31.0	34.0	30.0	34.0
4	35.884463954052684	37.0	35.0	37.0	35.0	37.0
5	32.16405585780853	37.0	35.0	37.0	0.0	37.0
6	34.01516130091625	37.0	35.0	37.0	19.0	37.0
7	20.645911557253523	32.0	0.0	37.0	0.0	37.0
8	28.038332001474167	35.0	17.0	37.0	17.0	37.0
9	34.891732621062346	37.0	32.0	39.0	32.0	39.0
10	36.2705133678522	37.0	35.0	39.0	32.0	39.0
11	36.689592888966736	38.0	35.0	39.0	33.0	39.0
12	37.017624328140904	39.0	37.0	39.0	34.0	39.0
13	36.806427953716984	39.0	37.0	39.0	33.0	39.0
14	38.12948590236195	40.0	38.0	41.0	33.0	41.0
15	38.32153999408874	40.0	38.0	41.0	34.0	41.0
16	38.4272896118634	40.0	38.0	41.0	34.0	41.0
17	38.40934052902176	40.0	38.0	41.0	34.0	41.0
18	38.30220066921362	40.0	37.0	41.0	34.0	41.0
19	38.26277763790216	40.0	37.0	41.0	34.0	41.0
20	38.23832616318741	40.0	37.0	41.0	34.0	41.0
21	38.142622047560145	40.0	37.0	41.0	34.0	41.0
22	38.14177184705148	40.0	37.0	41.0	34.0	41.0
23	38.04978963923037	40.0	37.0	41.0	34.0	41.0
24	37.93465132656822	40.0	36.0	41.0	33.0	41.0
25	37.78325725315906	40.0	36.0	41.0	33.0	41.0
26	37.64477674026557	40.0	36.0	41.0	33.0	41.0
27	37.61959183077726	40.0	36.0	41.0	33.0	41.0
28	37.54180395762863	40.0	36.0	41.0	33.0	41.0
29	37.50000912232306	40.0	36.0	41.0	33.0	41.0
30	37.33419083170044	40.0	36.0	41.0	33.0	41.0
31	37.22175272666236	40.0	35.0	41.0	32.0	41.0
32	37.10769084812062	39.0	35.0	41.0	32.0	41.0
33	36.98086866409052	39.0	35.0	41.0	31.0	41.0
34	36.81480954413927	39.0	35.0	41.0	31.0	41.0
35	36.68072234202873	39.0	35.0	41.0	30.0	41.0
36	36.53024414985423	39.0	35.0	41.0	30.0	41.0
37	36.4533466154357	39.0	35.0	41.0	30.0	41.0
38	36.32315282080474	39.0	35.0	41.0	30.0	41.0
39	36.208780783279146	39.0	35.0	41.0	29.0	41.0
40	36.07836805289488	39.0	35.0	41.0	29.0	41.0
41	35.95127584810238	39.0	35.0	40.0	28.0	41.0
42	35.854659500169674	39.0	35.0	40.0	28.0	41.0
43	35.77256589053942	39.0	35.0	40.0	28.0	41.0
44	35.622372314844206	38.0	35.0	40.0	27.0	41.0
45	35.53085352103425	38.0	35.0	40.0	27.0	41.0
46	35.446121735576696	38.0	34.0	40.0	27.0	41.0
47	35.39787194447789	38.0	34.0	40.0	27.0	41.0
48	35.259756324506576	38.0	34.0	40.0	26.0	41.0
49	35.17007294209514	38.0	34.0	40.0	26.0	41.0
50	34.99807701430015	38.0	34.0	40.0	26.0	41.0
51	33.27859574607832	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	16.0
10	8.0
11	11.0
12	5.0
13	6.0
14	6.0
15	15.0
16	39.0
17	55.0
18	121.0
19	167.0
20	354.0
21	575.0
22	828.0
23	1419.0
24	1880.0
25	2670.0
26	3049.0
27	3387.0
28	3815.0
29	4461.0
30	5301.0
31	6835.0
32	9165.0
33	13478.0
34	18939.0
35	24124.0
36	31928.0
37	52819.0
38	54122.0
39	34448.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.335435116565044	18.544952983546978	23.21704195903712	16.902569940850857
2	31.481501753310493	24.294570758211005	26.596315311271905	17.627612177206597
3	32.10108993515853	23.889904507522267	27.563646447949846	16.445359109369356
4	28.055157214115518	26.12961726381393	27.33449369282584	18.48073182924471
5	22.168704593637	37.81896202559359	23.53595837301544	16.476375007753973
6	26.21098838545829	35.51393343623314	24.735726301116937	13.539351877191638
7	50.22714584405207	43.154791226514575	4.582690209558005	2.0353727198753524
8	87.79652111088001	3.597114426771464	6.511514196159138	2.0948502661893866
9	82.2497838009436	5.893020693077617	8.351304309750304	3.5058911962284665
10	42.32320025688462	28.85901632166041	15.9341441254064	12.883639296048575
11	31.46727092934578	26.456561322080034	24.726239085140467	17.349928663433715
12	28.590090238019656	23.94171930247069	28.562358375934583	18.905832083575074
13	24.84811332114591	25.600522526664548	30.111328830554672	19.440035321634866
14	21.79651381302157	26.982736915852044	30.550295015927574	20.670454255198813
15	20.61316606641781	26.07561311133248	33.45958628440484	19.85163453784487
16	25.268834860410212	24.67916789818028	30.92941876206427	19.122578479345236
17	25.59906295497586	24.44125771292414	28.90718218738711	21.052497144712884
18	25.838067818998518	24.273042075802856	29.348337730293046	20.540552374905584
19	25.49725782968988	26.23762556877684	27.689534506099182	20.575582095434093
20	26.988575202606796	26.25331596443024	27.892414970826813	18.86569386213616
21	26.100425830040173	25.87747625459309	28.813404706388912	19.208693208977827
22	26.270830824694492	23.4648042531919	28.824351494054067	21.440013428059537
23	24.093149865172066	25.614388457707086	29.658496714139236	20.63396496298161
24	23.048826321915833	25.413697350512493	30.733471262857915	20.80400506471376
25	23.509321189696884	26.65907689388549	28.754656945919223	21.076944970498406
26	22.94300737448596	27.682601540577917	27.693183435320908	21.68120764961522
27	22.227087461184517	27.026524066512682	29.388475951731962	21.35791252057084
28	21.894670009085836	26.981277344163356	29.71578490292024	21.40826774383057
29	22.73246415839272	26.196757561493577	29.168080626740085	21.902697653373618
30	23.630830532780156	25.844635891597612	29.420586528883096	21.103947046739137
31	24.354413197447208	26.199311811948782	27.948608480841298	21.497666509762713
32	24.264284645670728	26.8517403567923	28.163165519078426	20.720809478458545
33	24.60436484913502	27.17248123538148	27.609987849065693	20.61316606641781
34	22.836093748289564	26.803209598143425	29.165526376284877	21.195170277282134
35	22.946656303707677	27.557808161195098	28.328097119900164	21.16743841519706
36	24.62625842446534	27.7479173736467	26.8269276380846	20.79889656380335
37	23.48158932761181	28.755021838841394	27.083812255293683	20.679576578253112
38	23.530120086260688	27.79571834645123	27.51657526098966	21.157586306298416
39	23.09772197348688	27.49212743520414	27.18890141687922	22.221249174429765
40	24.519709691191117	26.856483964780537	27.676763253823168	20.947043090205177
41	22.261752288790852	26.776207521902695	28.204033526361687	22.75800666294476
42	23.089329436276927	26.33833601529631	28.021587065275693	22.55074748315107
43	22.893017044148394	26.287615899114403	28.18615377317526	22.63321328356194
44	22.615333530375512	27.044038926776935	28.405089526478456	21.935538016369097
45	22.032599533666847	27.437758389800514	27.565106019638534	22.964536056894104
46	22.505865653723912	27.392146774529014	27.742079086891952	22.359908484855122
47	22.385086096484986	27.32902029899326	28.386844880369853	21.8990487241519
48	22.60037292056646	27.619110172119992	28.37151937763863	21.408997529674917
49	22.803983171138427	27.555983696584235	28.486460648122808	21.153572484154527
50	21.531236658602534	28.54739776612553	28.239793032734546	21.681572542537392
51	22.210302386764607	27.633705889006873	27.420973315380603	22.735018408847925
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	224.0
1	538.0
2	852.0
3	1803.0
4	2754.0
5	1932.5
6	1111.0
7	999.0
8	887.0
9	880.5
10	874.0
11	898.0
12	922.0
13	921.5
14	921.0
15	873.5
16	826.0
17	880.5
18	935.0
19	976.0
20	1017.0
21	1092.0
22	1167.0
23	1358.5
24	1550.0
25	1909.5
26	2783.0
27	3297.0
28	3850.5
29	4404.0
30	5198.0
31	5992.0
32	6816.5
33	7641.0
34	8353.0
35	9065.0
36	9812.5
37	10560.0
38	11326.0
39	12092.0
40	13275.0
41	14458.0
42	15161.0
43	15864.0
44	16485.5
45	17107.0
46	17401.5
47	17696.0
48	17927.5
49	18159.0
50	18201.5
51	18244.0
52	17767.0
53	17290.0
54	16441.0
55	15592.0
56	15108.0
57	14624.0
58	14019.0
59	13414.0
60	12439.0
61	11464.0
62	10262.5
63	9061.0
64	8087.5
65	7114.0
66	6177.0
67	5240.0
68	4414.5
69	3589.0
70	2871.0
71	2153.0
72	1808.5
73	1464.0
74	1197.0
75	752.0
76	574.0
77	433.0
78	292.0
79	227.5
80	163.0
81	129.5
82	96.0
83	75.0
84	54.0
85	40.5
86	27.0
87	20.0
88	13.0
89	9.0
90	5.0
91	2.5
92	0.0
93	1.5
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	274053.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.88799364932112
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.991609166538055	29.364465550439366
2	12.02457464484171	11.035672098765682
3	7.228877796382537	9.951560952363607
4	4.903628471054388	9.00070688553496
5	3.4941795567813725	8.017044465558563
6	2.453144245727675	6.754192054129211
7	1.8511572955381896	5.9462125955085625
8	1.2982280190128952	4.7658463273467575
9	0.8507281673268419	3.5134387865633436
>10	1.8918228287767802	10.81444408713111
>50	0.008836525881018693	0.25103544730043503
>100	0.002409961603914189	0.2875295872163573
>500	8.03320534638063E-4	0.2978511621420727
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	808	0.2948334811149668	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	488	0.17806774601993045	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007662751365611761	0.0
2	0.0	0.0	0.0	0.037948863905886816	0.0
3	0.0	0.0	0.0	0.04889565157104648	0.0
4	0.0	0.0	0.0	0.06312647553575404	0.0
5	0.0	0.0	0.0	0.10654873327422068	0.0
6	0.0	0.0	0.0	0.1769730672534145	0.0
7	0.0	0.0	0.0	0.22221978960274108	0.0
8	0.0	0.0	0.0	0.35467592035117296	0.0
9	0.0	0.0	0.0	0.4199917534199589	0.0
10	0.0	0.0	0.0	0.546244704491467	0.0
11	0.0	0.0	0.0	0.707892269013658	0.0
12	0.0	0.0	0.0	0.8523898661937654	0.0
13	0.0	0.0	0.0	0.9100429478969396	0.0
14	0.0	0.0	0.0	0.9472620259584825	0.0
15	0.0	0.0	0.0	0.995062998763013	0.0
16	0.0	0.0	0.0	1.0757043345630224	0.0
17	0.0	0.0	0.0	1.1764147810824914	0.0
18	0.0	0.0	0.0	1.2727465125358963	0.0
19	0.0	0.0	0.0	1.3420761677485742	0.0
20	0.0	0.0	0.0	1.4165143238716598	0.0
21	0.0	0.0	0.0	1.5026290535042492	0.0
22	0.0	0.0	0.0	1.5949469628137622	0.0
23	0.0	0.0	0.0	1.6923733730336832	0.0
24	0.0	0.0	0.0	1.7828668177323364	0.0
25	0.0	0.0	0.0	1.8711709048979577	0.0
26	0.0	0.0	0.0	1.9496228831649351	0.0
27	0.0	0.0	0.0	2.0470492933848563	0.0
28	0.0	0.0	0.0	2.1371778451613372	0.0
29	0.0	0.0	0.0	2.239712756291666	0.0
30	0.0	0.0	0.0	2.3681550648962064	0.0
31	0.0	0.0	0.0	2.465581475116127	0.0
32	0.0	0.0	0.0	2.5717653154681757	0.0
33	0.0	0.0	0.0	2.6794087275089127	0.0
34	0.0	0.0	0.0	2.7870521395496493	0.0
35	0.0	0.0	0.0	2.9293603791967247	0.0
36	0.0	0.0	0.0	3.048315471824793	0.0
37	0.0	0.0	0.0	3.1833258530284287	0.0
38	0.0	0.0	0.0	3.3318372723524283	0.0
39	3.6489292217198863E-4	0.0	0.0	3.6095207861253114	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTAAG	20	7.023874E-4	45.000004	2
CCCCATA	20	7.023874E-4	45.000004	32
AGTAGCG	20	7.023874E-4	45.000004	1
GGCGTAG	25	3.8830072E-5	45.0	1
ATAGCGG	25	3.8830072E-5	45.0	2
CGAATAT	25	3.8830072E-5	45.0	14
GCTACGA	25	3.8830072E-5	45.0	10
AGCTACG	25	3.8830072E-5	45.0	9
AGCGACT	55	6.002665E-11	40.909092	19
TTACGGG	50	1.0750227E-9	40.5	3
GGCCGAT	40	3.4453842E-7	39.375004	8
CGACCAA	80	0.0	39.375004	29
GTCAAGC	80	0.0	39.375004	16
ACGACCA	80	0.0	39.375004	28
TAGCGGG	115	0.0	39.130432	3
CACGGGC	35	6.23089E-6	38.571426	4
CACGAGG	35	6.23089E-6	38.571426	3
CGTTATT	70	0.0	38.571426	1
GCAATCG	35	6.23089E-6	38.571426	21
CAATCGG	35	6.23089E-6	38.571426	22
>>END_MODULE