##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934670.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 293538 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92605727367496 31.0 31.0 34.0 30.0 34.0 2 32.11650280372558 33.0 31.0 34.0 30.0 34.0 3 31.908373021550872 33.0 31.0 34.0 30.0 34.0 4 35.7337107972392 37.0 35.0 37.0 35.0 37.0 5 32.13714067684593 37.0 35.0 37.0 10.0 37.0 6 33.97737941935967 37.0 35.0 37.0 19.0 37.0 7 20.670502626576457 32.0 0.0 37.0 0.0 37.0 8 28.070825583059094 35.0 17.0 37.0 17.0 37.0 9 34.86720288344269 37.0 32.0 39.0 32.0 39.0 10 36.176890215236185 37.0 35.0 39.0 32.0 39.0 11 36.61989589082163 38.0 35.0 39.0 33.0 39.0 12 36.96823239239894 39.0 37.0 39.0 33.0 39.0 13 36.704903624062304 39.0 35.0 39.0 33.0 39.0 14 38.07932192765502 40.0 37.0 41.0 33.0 41.0 15 38.25796660057642 40.0 37.0 41.0 33.0 41.0 16 38.323402762163674 40.0 37.0 41.0 34.0 41.0 17 38.29754920998303 40.0 37.0 41.0 34.0 41.0 18 38.2169259175984 40.0 37.0 41.0 34.0 41.0 19 38.151063916767164 40.0 37.0 41.0 34.0 41.0 20 38.10812910083192 40.0 37.0 41.0 34.0 41.0 21 38.01621595841084 40.0 36.0 41.0 34.0 41.0 22 37.967251258780806 40.0 36.0 41.0 34.0 41.0 23 37.8979689171419 40.0 36.0 41.0 33.0 41.0 24 37.76350251074818 40.0 36.0 41.0 33.0 41.0 25 37.65290013558722 39.0 35.0 41.0 33.0 41.0 26 37.47065797273266 39.0 35.0 41.0 33.0 41.0 27 37.44117967690725 39.0 35.0 41.0 33.0 41.0 28 37.384427229183274 39.0 35.0 41.0 33.0 41.0 29 37.3848428482854 39.0 35.0 41.0 33.0 41.0 30 37.221419373300904 39.0 35.0 41.0 33.0 41.0 31 37.088717644734245 39.0 35.0 41.0 32.0 41.0 32 36.98395437728676 39.0 35.0 41.0 32.0 41.0 33 36.80800100838733 39.0 35.0 41.0 31.0 41.0 34 36.59363012625282 39.0 35.0 41.0 30.0 41.0 35 36.441356826032745 39.0 35.0 41.0 30.0 41.0 36 36.27408376428265 39.0 35.0 41.0 30.0 41.0 37 36.188827340923496 39.0 35.0 40.0 30.0 41.0 38 36.08491234525002 39.0 35.0 40.0 29.0 41.0 39 36.00380189277027 39.0 35.0 40.0 29.0 41.0 40 35.881293052347566 39.0 35.0 40.0 28.0 41.0 41 35.79441162643337 39.0 35.0 40.0 28.0 41.0 42 35.681325756801506 38.0 35.0 40.0 27.0 41.0 43 35.60447029004762 38.0 35.0 40.0 27.0 41.0 44 35.43940137222438 38.0 35.0 40.0 26.0 41.0 45 35.34585641382036 38.0 34.0 40.0 26.0 41.0 46 35.210528108796815 38.0 34.0 40.0 26.0 41.0 47 35.18224216285456 38.0 34.0 40.0 26.0 41.0 48 35.05317199136057 38.0 34.0 40.0 25.0 41.0 49 34.92782195150202 38.0 34.0 40.0 24.0 41.0 50 34.743389271576426 38.0 34.0 40.0 24.0 41.0 51 32.87514393366447 36.0 31.0 39.0 19.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 7.0 9 15.0 10 9.0 11 10.0 12 3.0 13 9.0 14 12.0 15 13.0 16 17.0 17 49.0 18 96.0 19 187.0 20 327.0 21 554.0 22 847.0 23 1411.0 24 2192.0 25 3018.0 26 3682.0 27 4069.0 28 4480.0 29 4962.0 30 6143.0 31 7893.0 32 10612.0 33 15765.0 34 21883.0 35 27625.0 36 33270.0 37 54315.0 38 55428.0 39 34628.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.86093793648522 18.979144097186737 23.908318514127643 17.2515994522004 2 32.48608357350667 25.050249030789878 25.925774516416954 16.5378928792865 3 31.00245964747324 23.6698485374977 29.053478595616237 16.274213219412818 4 28.18101915254584 25.59941131982912 27.646165062104394 18.57340446552065 5 21.430615456942544 36.76219092587672 24.78214064277879 17.02505297440195 6 25.906015575496188 34.49740749068263 26.459947264068024 13.13662966975315 7 48.6935251994631 43.056094951931264 5.919165491350354 2.331214357255279 8 84.87316803957239 4.065231758750145 8.636019867955767 2.425580333721699 9 79.30046535712583 6.443458768541041 10.412280522453651 3.8437953518794843 10 42.446974497339355 25.649149343526222 18.555008210180624 13.3488679489538 11 32.1341700222799 25.358556643432877 25.803473485545315 16.7037998487419 12 30.21721208157036 22.275139845607725 29.21461616553904 18.293031907282874 13 23.569009804522754 26.23817018580218 30.64373266834277 19.549087341332296 14 20.120052599663417 27.441762224993017 31.753980745252747 20.684204430090823 15 20.0730399471278 25.411020038291465 35.05713059297263 19.458809421608105 16 22.368824479283774 23.783973454884887 34.71714054057737 19.13006152525397 17 22.515994522003965 24.269430193024412 30.60796217184828 22.606613113123345 18 23.949199081549917 24.575012434505926 31.440903733077146 20.034884750867008 19 23.752291015132624 27.144015425600777 28.900176467782707 20.203517091483896 20 25.520375556146053 25.357193957852136 30.020304015153066 19.10212647084875 21 24.143041105410543 26.84558728341816 29.90583842637069 19.105533184800606 22 23.85858049043054 24.476217729902093 29.561419645838015 22.10378213382935 23 22.375637907187485 26.02116250706893 30.55107004885228 21.052129536891307 24 21.790023778863386 24.336542457875982 33.54591228392917 20.327521479331466 25 21.160803711955523 25.658006799801047 31.253193794329864 21.927995693913566 26 20.473328836470916 27.70339785649558 30.285346360607484 21.537926946426015 27 20.02636796598737 27.7006724853341 31.30395383221252 20.96900571646601 28 18.886140806301057 27.859766026885787 32.39853102494396 20.855562141869196 29 20.40996395696639 25.679128426302555 32.451335091197734 21.45957252553332 30 20.490703077625383 26.753265335322858 31.99585743583454 20.76017415121722 31 23.31350625813353 26.143463537940576 29.285135144342473 21.257895059583426 32 23.625901927518754 26.253500398585533 29.527011834924267 20.593585838971446 33 22.825664820227704 27.192390763717135 28.834086217116695 21.14785819893847 34 20.69238054357528 26.669119500712 31.032438730249577 21.606061225463144 35 21.140363428244385 26.51002595916031 30.541871921182267 21.807738691413036 36 22.48294939667096 27.897239880356206 29.03099428353399 20.588816439438844 37 22.18860931123057 28.345222765025312 29.78490008107979 19.681267842664322 38 21.255169688421944 27.842732457126505 28.88178021244268 22.020317642008873 39 21.71780144308403 27.059869590989923 30.034952885146044 21.187376080780002 40 22.738112271665 26.45006779360764 29.253452704590206 21.558367230137154 41 20.003202311114745 26.119957211672766 30.532333122117066 23.344507355095423 42 21.120604487323618 25.703316095360734 29.443206671708605 23.732872745607043 43 21.90040131090353 25.079206099380656 29.570277102112847 23.450115487602968 44 20.63310372081298 26.379548814804217 29.421744373811908 23.565603090570896 45 20.79832934747801 27.93437306243144 28.077795719804595 23.18950187028596 46 21.403361745327693 28.46479842473547 28.169095653714336 21.962744176222497 47 20.829330444439904 27.134135955140394 29.915036554040704 22.121497046379005 48 21.46434192506592 25.469274846868206 31.047428271637745 22.01895495642813 49 21.4980683931893 26.152661665610584 29.844858246632462 22.504411694567654 50 19.991960155073617 27.526248730999054 30.078899495124993 22.402891618802336 51 19.792326717494838 26.840477212490377 28.987388344950226 24.379807725064556 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 256.0 1 615.0 2 974.0 3 2847.0 4 4720.0 5 3237.5 6 1755.0 7 1531.5 8 1308.0 9 1294.0 10 1280.0 11 1293.0 12 1306.0 13 1338.0 14 1370.0 15 1334.0 16 1298.0 17 1239.0 18 1180.0 19 1288.0 20 1396.0 21 1438.5 22 1481.0 23 1630.0 24 1779.0 25 2197.0 26 3128.0 27 3641.0 28 4461.5 29 5282.0 30 5839.5 31 6397.0 32 7126.0 33 7855.0 34 8838.0 35 9821.0 36 10685.0 37 11549.0 38 12482.0 39 13415.0 40 14620.0 41 15825.0 42 17203.0 43 18581.0 44 19303.5 45 20026.0 46 20572.5 47 21119.0 48 21121.0 49 21123.0 50 20219.0 51 19315.0 52 18243.5 53 17172.0 54 16040.5 55 14909.0 56 14206.5 57 13504.0 58 12983.0 59 12462.0 60 11780.0 61 11098.0 62 10087.0 63 9076.0 64 7594.5 65 6113.0 66 5110.0 67 4107.0 68 3531.0 69 2955.0 70 2479.5 71 2004.0 72 1735.5 73 1467.0 74 1188.5 75 708.0 76 506.0 77 373.0 78 240.0 79 199.5 80 159.0 81 117.0 82 75.0 83 62.5 84 50.0 85 35.0 86 20.0 87 18.5 88 17.0 89 15.5 90 14.0 91 9.0 92 4.0 93 3.5 94 3.0 95 4.0 96 5.0 97 2.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 293538.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.10596645459798 #Duplication Level Percentage of deduplicated Percentage of total 1 70.73611086504886 31.906206438076172 2 9.19658992173497 8.296421530129429 3 4.940645663437235 6.685577926771648 4 3.341050052814307 6.028051664214998 5 2.5217916340729967 5.68739244259912 6 2.047934195252507 5.542443066737022 7 1.5089266432440676 4.764311618683124 8 1.2934796822228976 4.667492092484006 9 0.9726919128061485 3.9486787910723598 >10 3.4195026047238555 20.74251575788336 >50 0.015957618481619716 0.4795131150924616 >100 0.0037994329718142182 0.4010224050451609 >500 7.598865943628435E-4 0.3119063150351251 >1k 7.598865943628435E-4 0.5384668361760236 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1571 0.5351947618366276 No Hit CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 910 0.310010969618925 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 375 0.12775177319461192 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.013967527202610906 0.0 2 0.0 0.0 0.0 0.08482717740122232 0.0 3 0.0 0.0 0.0 0.12979580156572573 0.0 4 0.0 0.0 0.0 0.17408308293985786 0.0 5 0.0 0.0 0.0 0.35566093657379966 0.0 6 0.0 0.0 0.0 0.521908577424388 0.0 7 0.0 0.0 0.0 0.6636278778216108 0.0 8 0.0 0.0 0.0 1.0029365874265002 0.0 9 0.0 0.0 0.0 1.1466999161948368 0.0 10 0.0 0.0 0.0 1.5422194060053553 0.0 11 0.0 0.0 0.0 2.115569364102774 0.0 12 0.0 0.0 0.0 2.5253970525110887 0.0 13 0.0 0.0 0.0 2.6998208068461325 0.0 14 0.0 0.0 0.0 2.772043142625486 0.0 15 0.0 0.0 0.0 2.9076303579093676 0.0 16 0.0 0.0 0.0 3.220366698689778 0.0 17 0.0 0.0 0.0 3.59612724757953 0.0 18 0.0 0.0 0.0 3.98994338041412 0.0 19 0.0 0.0 0.0 4.2209185863499785 0.0 20 0.0 0.0 0.0 4.444399021591753 0.0 21 0.0 0.0 0.0 4.765311475856619 0.0 22 0.0 0.0 0.0 5.095422057791495 0.0 23 0.0 0.0 0.0 5.432005396234899 0.0 24 0.0 0.0 0.0 5.727708167256028 0.0 25 0.0 0.0 0.0 5.991387827129707 0.0 26 0.0 0.0 0.0 6.229176460969279 0.0 27 0.0 0.0 0.0 6.469009123179963 0.0 28 0.0 0.0 0.0 6.715314541899175 0.0 29 0.0 0.0 0.0 6.9711587596835844 0.0 30 0.0 0.0 0.0 7.286961143020665 0.0 31 0.0 0.0 0.0 7.587773984969578 0.0 32 0.0 0.0 0.0 7.87768534227255 0.0 33 0.0 0.0 0.0 8.166915356785152 0.0 34 0.0 0.0 0.0 8.475904312218521 0.0 35 0.0 0.0 0.0 8.768200369287792 0.0 36 0.0 0.0 0.0 9.062199783332993 0.0 37 0.0 0.0 0.0 9.372892095742289 0.0 38 0.0 0.0 0.0 9.692782535821598 0.0 39 0.0 0.0 0.0 10.029706545660186 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTCGGT 25 3.8836843E-5 45.000004 24 GGGCGTA 25 3.8836843E-5 45.000004 8 CGATATA 30 2.159959E-6 45.000004 10 TGTCGCA 25 3.8836843E-5 45.000004 39 GTACGGG 45 3.8198777E-10 45.000004 3 CCGTGAC 20 7.0246944E-4 45.0 42 TACGCGG 20 7.0246944E-4 45.0 2 TGCGCAA 20 7.0246944E-4 45.0 1 GGTATAC 20 7.0246944E-4 45.0 8 CGAATAT 55 1.8189894E-12 45.0 14 TTAACGG 20 7.0246944E-4 45.0 2 ACTCGCT 20 7.0246944E-4 45.0 18 TTCGGTA 20 7.0246944E-4 45.0 25 TTACGCG 20 7.0246944E-4 45.0 1 CGTTTTA 315 0.0 43.57143 1 AGGCGAT 165 0.0 42.272728 7 AAGGCGA 80 0.0 42.1875 6 AGGACCG 55 6.002665E-11 40.909092 7 CGAGACA 55 6.002665E-11 40.909092 22 CGTTTTT 585 0.0 40.384617 1 >>END_MODULE