##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934670.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	293538
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92605727367496	31.0	31.0	34.0	30.0	34.0
2	32.11650280372558	33.0	31.0	34.0	30.0	34.0
3	31.908373021550872	33.0	31.0	34.0	30.0	34.0
4	35.7337107972392	37.0	35.0	37.0	35.0	37.0
5	32.13714067684593	37.0	35.0	37.0	10.0	37.0
6	33.97737941935967	37.0	35.0	37.0	19.0	37.0
7	20.670502626576457	32.0	0.0	37.0	0.0	37.0
8	28.070825583059094	35.0	17.0	37.0	17.0	37.0
9	34.86720288344269	37.0	32.0	39.0	32.0	39.0
10	36.176890215236185	37.0	35.0	39.0	32.0	39.0
11	36.61989589082163	38.0	35.0	39.0	33.0	39.0
12	36.96823239239894	39.0	37.0	39.0	33.0	39.0
13	36.704903624062304	39.0	35.0	39.0	33.0	39.0
14	38.07932192765502	40.0	37.0	41.0	33.0	41.0
15	38.25796660057642	40.0	37.0	41.0	33.0	41.0
16	38.323402762163674	40.0	37.0	41.0	34.0	41.0
17	38.29754920998303	40.0	37.0	41.0	34.0	41.0
18	38.2169259175984	40.0	37.0	41.0	34.0	41.0
19	38.151063916767164	40.0	37.0	41.0	34.0	41.0
20	38.10812910083192	40.0	37.0	41.0	34.0	41.0
21	38.01621595841084	40.0	36.0	41.0	34.0	41.0
22	37.967251258780806	40.0	36.0	41.0	34.0	41.0
23	37.8979689171419	40.0	36.0	41.0	33.0	41.0
24	37.76350251074818	40.0	36.0	41.0	33.0	41.0
25	37.65290013558722	39.0	35.0	41.0	33.0	41.0
26	37.47065797273266	39.0	35.0	41.0	33.0	41.0
27	37.44117967690725	39.0	35.0	41.0	33.0	41.0
28	37.384427229183274	39.0	35.0	41.0	33.0	41.0
29	37.3848428482854	39.0	35.0	41.0	33.0	41.0
30	37.221419373300904	39.0	35.0	41.0	33.0	41.0
31	37.088717644734245	39.0	35.0	41.0	32.0	41.0
32	36.98395437728676	39.0	35.0	41.0	32.0	41.0
33	36.80800100838733	39.0	35.0	41.0	31.0	41.0
34	36.59363012625282	39.0	35.0	41.0	30.0	41.0
35	36.441356826032745	39.0	35.0	41.0	30.0	41.0
36	36.27408376428265	39.0	35.0	41.0	30.0	41.0
37	36.188827340923496	39.0	35.0	40.0	30.0	41.0
38	36.08491234525002	39.0	35.0	40.0	29.0	41.0
39	36.00380189277027	39.0	35.0	40.0	29.0	41.0
40	35.881293052347566	39.0	35.0	40.0	28.0	41.0
41	35.79441162643337	39.0	35.0	40.0	28.0	41.0
42	35.681325756801506	38.0	35.0	40.0	27.0	41.0
43	35.60447029004762	38.0	35.0	40.0	27.0	41.0
44	35.43940137222438	38.0	35.0	40.0	26.0	41.0
45	35.34585641382036	38.0	34.0	40.0	26.0	41.0
46	35.210528108796815	38.0	34.0	40.0	26.0	41.0
47	35.18224216285456	38.0	34.0	40.0	26.0	41.0
48	35.05317199136057	38.0	34.0	40.0	25.0	41.0
49	34.92782195150202	38.0	34.0	40.0	24.0	41.0
50	34.743389271576426	38.0	34.0	40.0	24.0	41.0
51	32.87514393366447	36.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	15.0
10	9.0
11	10.0
12	3.0
13	9.0
14	12.0
15	13.0
16	17.0
17	49.0
18	96.0
19	187.0
20	327.0
21	554.0
22	847.0
23	1411.0
24	2192.0
25	3018.0
26	3682.0
27	4069.0
28	4480.0
29	4962.0
30	6143.0
31	7893.0
32	10612.0
33	15765.0
34	21883.0
35	27625.0
36	33270.0
37	54315.0
38	55428.0
39	34628.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.86093793648522	18.979144097186737	23.908318514127643	17.2515994522004
2	32.48608357350667	25.050249030789878	25.925774516416954	16.5378928792865
3	31.00245964747324	23.6698485374977	29.053478595616237	16.274213219412818
4	28.18101915254584	25.59941131982912	27.646165062104394	18.57340446552065
5	21.430615456942544	36.76219092587672	24.78214064277879	17.02505297440195
6	25.906015575496188	34.49740749068263	26.459947264068024	13.13662966975315
7	48.6935251994631	43.056094951931264	5.919165491350354	2.331214357255279
8	84.87316803957239	4.065231758750145	8.636019867955767	2.425580333721699
9	79.30046535712583	6.443458768541041	10.412280522453651	3.8437953518794843
10	42.446974497339355	25.649149343526222	18.555008210180624	13.3488679489538
11	32.1341700222799	25.358556643432877	25.803473485545315	16.7037998487419
12	30.21721208157036	22.275139845607725	29.21461616553904	18.293031907282874
13	23.569009804522754	26.23817018580218	30.64373266834277	19.549087341332296
14	20.120052599663417	27.441762224993017	31.753980745252747	20.684204430090823
15	20.0730399471278	25.411020038291465	35.05713059297263	19.458809421608105
16	22.368824479283774	23.783973454884887	34.71714054057737	19.13006152525397
17	22.515994522003965	24.269430193024412	30.60796217184828	22.606613113123345
18	23.949199081549917	24.575012434505926	31.440903733077146	20.034884750867008
19	23.752291015132624	27.144015425600777	28.900176467782707	20.203517091483896
20	25.520375556146053	25.357193957852136	30.020304015153066	19.10212647084875
21	24.143041105410543	26.84558728341816	29.90583842637069	19.105533184800606
22	23.85858049043054	24.476217729902093	29.561419645838015	22.10378213382935
23	22.375637907187485	26.02116250706893	30.55107004885228	21.052129536891307
24	21.790023778863386	24.336542457875982	33.54591228392917	20.327521479331466
25	21.160803711955523	25.658006799801047	31.253193794329864	21.927995693913566
26	20.473328836470916	27.70339785649558	30.285346360607484	21.537926946426015
27	20.02636796598737	27.7006724853341	31.30395383221252	20.96900571646601
28	18.886140806301057	27.859766026885787	32.39853102494396	20.855562141869196
29	20.40996395696639	25.679128426302555	32.451335091197734	21.45957252553332
30	20.490703077625383	26.753265335322858	31.99585743583454	20.76017415121722
31	23.31350625813353	26.143463537940576	29.285135144342473	21.257895059583426
32	23.625901927518754	26.253500398585533	29.527011834924267	20.593585838971446
33	22.825664820227704	27.192390763717135	28.834086217116695	21.14785819893847
34	20.69238054357528	26.669119500712	31.032438730249577	21.606061225463144
35	21.140363428244385	26.51002595916031	30.541871921182267	21.807738691413036
36	22.48294939667096	27.897239880356206	29.03099428353399	20.588816439438844
37	22.18860931123057	28.345222765025312	29.78490008107979	19.681267842664322
38	21.255169688421944	27.842732457126505	28.88178021244268	22.020317642008873
39	21.71780144308403	27.059869590989923	30.034952885146044	21.187376080780002
40	22.738112271665	26.45006779360764	29.253452704590206	21.558367230137154
41	20.003202311114745	26.119957211672766	30.532333122117066	23.344507355095423
42	21.120604487323618	25.703316095360734	29.443206671708605	23.732872745607043
43	21.90040131090353	25.079206099380656	29.570277102112847	23.450115487602968
44	20.63310372081298	26.379548814804217	29.421744373811908	23.565603090570896
45	20.79832934747801	27.93437306243144	28.077795719804595	23.18950187028596
46	21.403361745327693	28.46479842473547	28.169095653714336	21.962744176222497
47	20.829330444439904	27.134135955140394	29.915036554040704	22.121497046379005
48	21.46434192506592	25.469274846868206	31.047428271637745	22.01895495642813
49	21.4980683931893	26.152661665610584	29.844858246632462	22.504411694567654
50	19.991960155073617	27.526248730999054	30.078899495124993	22.402891618802336
51	19.792326717494838	26.840477212490377	28.987388344950226	24.379807725064556
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	256.0
1	615.0
2	974.0
3	2847.0
4	4720.0
5	3237.5
6	1755.0
7	1531.5
8	1308.0
9	1294.0
10	1280.0
11	1293.0
12	1306.0
13	1338.0
14	1370.0
15	1334.0
16	1298.0
17	1239.0
18	1180.0
19	1288.0
20	1396.0
21	1438.5
22	1481.0
23	1630.0
24	1779.0
25	2197.0
26	3128.0
27	3641.0
28	4461.5
29	5282.0
30	5839.5
31	6397.0
32	7126.0
33	7855.0
34	8838.0
35	9821.0
36	10685.0
37	11549.0
38	12482.0
39	13415.0
40	14620.0
41	15825.0
42	17203.0
43	18581.0
44	19303.5
45	20026.0
46	20572.5
47	21119.0
48	21121.0
49	21123.0
50	20219.0
51	19315.0
52	18243.5
53	17172.0
54	16040.5
55	14909.0
56	14206.5
57	13504.0
58	12983.0
59	12462.0
60	11780.0
61	11098.0
62	10087.0
63	9076.0
64	7594.5
65	6113.0
66	5110.0
67	4107.0
68	3531.0
69	2955.0
70	2479.5
71	2004.0
72	1735.5
73	1467.0
74	1188.5
75	708.0
76	506.0
77	373.0
78	240.0
79	199.5
80	159.0
81	117.0
82	75.0
83	62.5
84	50.0
85	35.0
86	20.0
87	18.5
88	17.0
89	15.5
90	14.0
91	9.0
92	4.0
93	3.5
94	3.0
95	4.0
96	5.0
97	2.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	293538.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.10596645459798
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.73611086504886	31.906206438076172
2	9.19658992173497	8.296421530129429
3	4.940645663437235	6.685577926771648
4	3.341050052814307	6.028051664214998
5	2.5217916340729967	5.68739244259912
6	2.047934195252507	5.542443066737022
7	1.5089266432440676	4.764311618683124
8	1.2934796822228976	4.667492092484006
9	0.9726919128061485	3.9486787910723598
>10	3.4195026047238555	20.74251575788336
>50	0.015957618481619716	0.4795131150924616
>100	0.0037994329718142182	0.4010224050451609
>500	7.598865943628435E-4	0.3119063150351251
>1k	7.598865943628435E-4	0.5384668361760236
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1571	0.5351947618366276	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	910	0.310010969618925	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	375	0.12775177319461192	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013967527202610906	0.0
2	0.0	0.0	0.0	0.08482717740122232	0.0
3	0.0	0.0	0.0	0.12979580156572573	0.0
4	0.0	0.0	0.0	0.17408308293985786	0.0
5	0.0	0.0	0.0	0.35566093657379966	0.0
6	0.0	0.0	0.0	0.521908577424388	0.0
7	0.0	0.0	0.0	0.6636278778216108	0.0
8	0.0	0.0	0.0	1.0029365874265002	0.0
9	0.0	0.0	0.0	1.1466999161948368	0.0
10	0.0	0.0	0.0	1.5422194060053553	0.0
11	0.0	0.0	0.0	2.115569364102774	0.0
12	0.0	0.0	0.0	2.5253970525110887	0.0
13	0.0	0.0	0.0	2.6998208068461325	0.0
14	0.0	0.0	0.0	2.772043142625486	0.0
15	0.0	0.0	0.0	2.9076303579093676	0.0
16	0.0	0.0	0.0	3.220366698689778	0.0
17	0.0	0.0	0.0	3.59612724757953	0.0
18	0.0	0.0	0.0	3.98994338041412	0.0
19	0.0	0.0	0.0	4.2209185863499785	0.0
20	0.0	0.0	0.0	4.444399021591753	0.0
21	0.0	0.0	0.0	4.765311475856619	0.0
22	0.0	0.0	0.0	5.095422057791495	0.0
23	0.0	0.0	0.0	5.432005396234899	0.0
24	0.0	0.0	0.0	5.727708167256028	0.0
25	0.0	0.0	0.0	5.991387827129707	0.0
26	0.0	0.0	0.0	6.229176460969279	0.0
27	0.0	0.0	0.0	6.469009123179963	0.0
28	0.0	0.0	0.0	6.715314541899175	0.0
29	0.0	0.0	0.0	6.9711587596835844	0.0
30	0.0	0.0	0.0	7.286961143020665	0.0
31	0.0	0.0	0.0	7.587773984969578	0.0
32	0.0	0.0	0.0	7.87768534227255	0.0
33	0.0	0.0	0.0	8.166915356785152	0.0
34	0.0	0.0	0.0	8.475904312218521	0.0
35	0.0	0.0	0.0	8.768200369287792	0.0
36	0.0	0.0	0.0	9.062199783332993	0.0
37	0.0	0.0	0.0	9.372892095742289	0.0
38	0.0	0.0	0.0	9.692782535821598	0.0
39	0.0	0.0	0.0	10.029706545660186	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGGT	25	3.8836843E-5	45.000004	24
GGGCGTA	25	3.8836843E-5	45.000004	8
CGATATA	30	2.159959E-6	45.000004	10
TGTCGCA	25	3.8836843E-5	45.000004	39
GTACGGG	45	3.8198777E-10	45.000004	3
CCGTGAC	20	7.0246944E-4	45.0	42
TACGCGG	20	7.0246944E-4	45.0	2
TGCGCAA	20	7.0246944E-4	45.0	1
GGTATAC	20	7.0246944E-4	45.0	8
CGAATAT	55	1.8189894E-12	45.0	14
TTAACGG	20	7.0246944E-4	45.0	2
ACTCGCT	20	7.0246944E-4	45.0	18
TTCGGTA	20	7.0246944E-4	45.0	25
TTACGCG	20	7.0246944E-4	45.0	1
CGTTTTA	315	0.0	43.57143	1
AGGCGAT	165	0.0	42.272728	7
AAGGCGA	80	0.0	42.1875	6
AGGACCG	55	6.002665E-11	40.909092	7
CGAGACA	55	6.002665E-11	40.909092	22
CGTTTTT	585	0.0	40.384617	1
>>END_MODULE