##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934669.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	343301
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.896676677318155	31.0	31.0	34.0	30.0	34.0
2	32.08958319375708	33.0	31.0	34.0	30.0	34.0
3	31.580819747102396	33.0	31.0	34.0	28.0	34.0
4	35.540799473348464	37.0	35.0	37.0	33.0	37.0
5	32.01187587568926	37.0	35.0	37.0	0.0	37.0
6	33.93217031118465	37.0	35.0	37.0	19.0	37.0
7	20.943405349824207	32.0	0.0	37.0	0.0	37.0
8	28.247036856869045	35.0	17.0	37.0	17.0	37.0
9	35.03090291027407	37.0	32.0	39.0	32.0	39.0
10	36.32341006871521	37.0	35.0	39.0	32.0	39.0
11	36.71617035779098	39.0	37.0	39.0	33.0	39.0
12	37.14968497033216	39.0	37.0	39.0	34.0	39.0
13	37.02340220389687	39.0	37.0	39.0	33.0	39.0
14	38.47476704116796	40.0	38.0	41.0	34.0	41.0
15	38.63404126408021	40.0	38.0	41.0	35.0	41.0
16	38.6702805992409	40.0	38.0	41.0	35.0	41.0
17	38.62675319908768	40.0	38.0	41.0	35.0	41.0
18	38.48564088074314	40.0	38.0	41.0	34.0	41.0
19	38.36853373570133	40.0	37.0	41.0	34.0	41.0
20	38.24860399474514	40.0	37.0	41.0	34.0	41.0
21	38.15507965313238	40.0	37.0	41.0	34.0	41.0
22	38.06778308248447	40.0	36.0	41.0	34.0	41.0
23	37.96914369605681	40.0	36.0	41.0	34.0	41.0
24	37.80951701276722	40.0	35.0	41.0	33.0	41.0
25	37.665826781745466	40.0	35.0	41.0	33.0	41.0
26	37.57175481574478	39.0	35.0	41.0	33.0	41.0
27	37.530697551128604	39.0	35.0	41.0	33.0	41.0
28	37.45650027235575	39.0	35.0	41.0	33.0	41.0
29	37.4464711725279	40.0	35.0	41.0	33.0	41.0
30	37.28710373695387	39.0	35.0	41.0	33.0	41.0
31	37.11880536322353	39.0	35.0	41.0	32.0	41.0
32	36.92223733691425	39.0	35.0	41.0	31.0	41.0
33	36.636263803484404	39.0	35.0	41.0	31.0	41.0
34	36.309605856085476	39.0	35.0	41.0	30.0	41.0
35	35.97878829365484	39.0	35.0	41.0	27.0	41.0
36	35.74459730673665	39.0	35.0	41.0	25.0	41.0
37	35.62450735651804	39.0	35.0	41.0	25.0	41.0
38	35.46986463773773	39.0	35.0	41.0	24.0	41.0
39	35.412387380170756	39.0	35.0	41.0	23.0	41.0
40	35.31615695847085	39.0	35.0	41.0	23.0	41.0
41	35.21452894107503	39.0	35.0	41.0	23.0	41.0
42	35.098418006355935	39.0	35.0	40.0	22.0	41.0
43	35.0089775444872	39.0	35.0	40.0	22.0	41.0
44	34.87961876021334	39.0	34.0	40.0	21.0	41.0
45	34.79680513601767	39.0	34.0	40.0	20.0	41.0
46	34.68138455757484	38.0	34.0	40.0	20.0	41.0
47	34.65612683912951	38.0	34.0	40.0	20.0	41.0
48	34.54494743679744	38.0	34.0	40.0	20.0	41.0
49	34.4498559573086	38.0	34.0	40.0	20.0	41.0
50	34.31962913012196	38.0	34.0	40.0	20.0	41.0
51	32.745846938983576	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	12.0
10	14.0
11	7.0
12	3.0
13	4.0
14	10.0
15	10.0
16	20.0
17	64.0
18	107.0
19	218.0
20	457.0
21	808.0
22	1313.0
23	2467.0
24	3946.0
25	5554.0
26	6691.0
27	6576.0
28	6383.0
29	6116.0
30	6689.0
31	8018.0
32	10460.0
33	14939.0
34	20575.0
35	27221.0
36	35456.0
37	63252.0
38	69506.0
39	46391.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.436544024048864	18.701372847734206	23.603193698824064	23.258889429392866
2	35.44527979819459	23.946332809983076	24.05352737102426	16.554860020798078
3	29.304604414202114	22.8630269064174	32.312460493852335	15.519908185528152
4	24.844087258703006	24.67746962578029	32.62151872554988	17.85692438996682
5	20.96644052886534	35.05320403960373	28.347135604032616	15.633219827498317
6	24.540563528798344	32.440045324656786	30.651236087282008	12.368155059262863
7	47.15162495885535	41.907247575742566	9.110663819796622	1.83046364560546
8	82.80109874425067	3.053005962697458	12.494866021363176	1.6510292716886932
9	77.31262070311476	6.175047553021984	13.84674090666791	2.6655908371953476
10	33.491891954873424	34.388772534889206	21.451728949231143	10.667606561006231
11	24.384723609893356	25.064884751282403	35.24108581099385	15.30930582783039
12	23.35792788252874	23.411525163049333	35.88367059810487	17.34687635631705
13	20.175006772482458	26.148773233984173	36.643062502002614	17.033157491530755
14	18.20851089859919	27.397240322632328	35.75637705686846	18.637871721900023
15	17.732252454842833	26.575512451172585	38.20320942846074	17.48902566552384
16	20.95944957923222	25.841171450126854	36.88483284348138	16.31454612715955
17	20.62825334036312	25.30548993448898	35.39255638637813	18.673700338769766
18	22.008965892904477	23.845546619438917	36.80123273745197	17.34425475020463
19	20.90643487784772	26.17440671597228	34.48955872543337	18.429599680746634
20	22.282778086868372	25.709799854937792	35.51693703193408	16.490485026259755
21	21.254234622095478	26.265580350770897	35.85716324741262	16.623021779721004
22	21.42696933594717	23.477939184563983	36.25593866606855	18.83915281342029
23	19.0456771171654	26.72436142044445	35.75229900291581	18.477662459474338
24	18.112676630711825	25.31568506937061	38.76714603219915	17.804492267718416
25	18.329687358906614	27.835339832974558	36.07563042344765	17.75934238467118
26	18.810897725319762	28.190421816423488	34.83473686356871	18.163943594688043
27	18.167439069504603	28.040407688879437	35.64889120625923	18.143262035356727
28	17.219874104648692	28.23236751422221	36.50673898415676	18.041019396972334
29	18.070148353777007	25.83126760481327	36.96144199987766	19.137142041532066
30	18.239678882380186	26.777667411396994	36.937847544865875	18.044806161356945
31	20.417068403529264	26.85922849045007	34.859787766420716	17.86391533959994
32	19.34687053052569	26.679794116533305	35.58422492215286	18.38911043078814
33	19.336092816507964	27.46831497723572	34.128359661055455	19.06723254520086
34	18.52368621122572	26.95622791660962	35.06194272664513	19.45814314551953
35	18.2428830676287	27.64104969108741	34.96028266739683	19.155784573887054
36	20.129856889435217	28.432483447470297	33.01767253809339	18.419987125001093
37	19.304050964022824	28.51579226393165	33.58918267060102	18.590974101444505
38	18.773903950177832	29.223625914285133	33.159239268164086	18.843230867372945
39	19.30346838488673	28.074197278772857	32.77968896099924	19.84264537534117
40	21.548145796254598	27.20149373290494	32.49772065913004	18.752639811710424
41	18.611946950343867	27.704259527353546	33.0520447071229	20.63174881517968
42	19.63349946548364	27.947777606240585	32.24196841838503	20.17675450989074
43	19.77827038080285	28.010696152938674	32.14467770265744	20.066355763601038
44	19.66350229099245	28.45258242766552	31.81581178033271	20.06810350100932
45	19.46513409515265	29.459861753971005	31.04010766062435	20.034896490251995
46	19.906146501175353	28.818442125132172	31.13361161196734	20.14179976172513
47	19.14821104511784	28.229745908109795	32.80357470557907	19.818468341193295
48	19.252201420910513	28.414714783819445	32.50209000265073	19.830993792619307
49	19.740111447388735	27.919522518140056	32.249250657586195	20.091115376885007
50	18.992662415780902	28.42490991870108	32.855424248691385	19.727003416826633
51	19.127238196218478	27.753196174785394	31.884556118391732	21.235009510604396
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	242.0
1	500.5
2	759.0
3	5944.5
4	11130.0
5	7740.0
6	4350.0
7	3817.5
8	3285.0
9	3135.5
10	2986.0
11	2871.5
12	2757.0
13	2683.0
14	2609.0
15	2528.5
16	2448.0
17	2411.5
18	2375.0
19	2373.5
20	2372.0
21	2556.5
22	2741.0
23	3054.5
24	3368.0
25	3845.0
26	5178.5
27	6035.0
28	6919.5
29	7804.0
30	8927.5
31	10051.0
32	11295.5
33	12540.0
34	13637.5
35	14735.0
36	16062.5
37	17390.0
38	18360.0
39	19330.0
40	20680.5
41	22031.0
42	22601.0
43	23171.0
44	23090.0
45	23009.0
46	22705.5
47	22402.0
48	22468.5
49	22535.0
50	22084.5
51	21634.0
52	20573.5
53	19513.0
54	17568.0
55	15623.0
56	13808.5
57	11994.0
58	10415.0
59	8836.0
60	7720.5
61	6605.0
62	5610.5
63	4616.0
64	3766.0
65	2916.0
66	2305.5
67	1695.0
68	1408.5
69	1122.0
70	907.5
71	693.0
72	584.0
73	475.0
74	416.5
75	299.0
76	240.0
77	173.5
78	107.0
79	77.0
80	47.0
81	36.0
82	25.0
83	18.5
84	12.0
85	10.5
86	9.0
87	5.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	343301.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.41936402911162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.926922835849	33.15890209388722
2	10.08706607022258	9.5664451593917
3	5.523806752915792	7.85806209728937
4	3.6904282000886988	6.999910329732206
5	2.775110362694434	6.579698425478367
6	2.0798704696359582	5.917568095984011
7	1.5598290502524006	5.177627108997142
8	1.189424179505905	4.512139052241433
9	0.9019356326736702	3.849229268692188
>10	2.250565607668545	13.699576397829594
>50	0.010653927265889306	0.33402797636801906
>100	0.0025068064155033664	0.26864883508602244
>500	6.267016038758416E-4	0.24457742397765098
>1k	0.0012534032077516832	1.8335877350450867
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3982	1.1599150599619574	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2188	0.6373415748861786	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	823	0.2397313145024337	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	442	0.1287499890766412	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9128956804669954E-4	0.0	0.0	0.009321266177494385	0.0
2	2.9128956804669954E-4	0.0	0.0	0.041945697798724736	0.0
3	2.9128956804669954E-4	0.0	0.0	0.0667053110826942	0.0
4	2.9128956804669954E-4	0.0	0.0	0.09466910961517735	0.0
5	2.9128956804669954E-4	0.0	0.0	0.1785605052126268	0.0
6	2.9128956804669954E-4	0.0	0.0	0.30468888817684775	0.0
7	2.9128956804669954E-4	0.0	0.0	0.40430992044881897	0.0
8	2.9128956804669954E-4	0.0	0.0	0.6140384094424426	0.0
9	2.9128956804669954E-4	0.0	0.0	0.7186113643712078	0.0
10	2.9128956804669954E-4	0.0	0.0	0.9251356681163178	0.0
11	2.9128956804669954E-4	0.0	0.0	1.1788488818849931	0.0
12	2.9128956804669954E-4	0.0	0.0	1.3731390237721417	0.0
13	2.9128956804669954E-4	0.0	0.0	1.467808133387319	0.0
14	2.9128956804669954E-4	0.0	0.0	1.517327359955258	0.0
15	2.9128956804669954E-4	0.0	0.0	1.5779155901089714	0.0
16	2.9128956804669954E-4	0.0	0.0	1.7066655791856127	0.0
17	2.9128956804669954E-4	0.0	0.0	1.8552232588894293	0.0
18	2.9128956804669954E-4	0.0	0.0	2.014558652610974	0.0
19	2.9128956804669954E-4	0.0	0.0	2.1205880553799727	0.0
20	2.9128956804669954E-4	0.0	0.0	2.217296191971477	0.0
21	2.9128956804669954E-4	0.0	0.0	2.348959076728585	0.0
22	2.9128956804669954E-4	0.0	0.0	2.5144115513791103	0.0
23	2.9128956804669954E-4	0.0	0.0	2.6734556555326083	0.0
24	2.9128956804669954E-4	0.0	0.0	2.8121094899228374	0.0
25	2.9128956804669954E-4	0.0	0.0	2.948433007768693	0.0
26	2.9128956804669954E-4	0.0	0.0	3.0707746263483067	0.0
27	2.9128956804669954E-4	0.0	0.0	3.202146221537368	0.0
28	0.0058257913609339905	0.0	0.0	3.3384697393832234	0.0
29	0.0058257913609339905	0.0	0.0	3.4934357895840678	0.0
30	0.0058257913609339905	0.0	0.0	3.699668803761131	0.0
31	0.0058257913609339905	0.0	0.0	3.8648299888436095	0.0
32	0.0058257913609339905	0.0	0.0	4.046012100168657	0.0
33	0.0058257913609339905	0.0	0.0	4.219329393156443	0.0
34	0.0058257913609339905	0.0	0.0	4.383907999102828	0.0
35	0.0058257913609339905	0.0	0.0	4.574702666173416	0.0
36	0.0058257913609339905	0.0	0.0	4.757049935770651	0.0
37	0.0058257913609339905	0.0	0.0	4.95658328988264	0.0
38	0.0058257913609339905	0.0	0.0	5.156407933562675	0.0
39	0.0058257913609339905	0.0	0.0	5.386526692319568	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAG	20	7.026367E-4	45.0	32
AAGCGAT	20	7.026367E-4	45.0	6
CGACAAT	25	3.8850718E-5	45.0	20
AGCGAAG	40	6.7902874E-9	45.0	2
TAGGCGT	20	7.026367E-4	45.0	6
CGGAGCA	20	7.026367E-4	45.0	26
AATACGG	20	7.026367E-4	45.0	2
TTCTCGG	20	7.026367E-4	45.0	42
TTTACGG	30	2.1610394E-6	44.999996	2
TAGGCGA	30	2.1610394E-6	44.999996	6
TCGACAA	30	2.1610394E-6	44.999996	19
CGTTTTA	810	0.0	44.722225	1
CGTTATT	415	0.0	43.915665	1
CGTTTTT	1625	0.0	43.33846	1
AGGCGAT	135	0.0	43.333332	7
GCGATGC	60	3.6379788E-12	41.249996	9
CGGTTTA	45	1.9215804E-8	40.0	1
CGAGGAT	45	1.9215804E-8	40.0	5
GGCGATA	85	0.0	39.705883	8
ACGGGTG	40	3.4487857E-7	39.375	5
>>END_MODULE