##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934667.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	316016
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91646625487317	31.0	31.0	34.0	30.0	34.0
2	32.10619082578097	33.0	31.0	34.0	30.0	34.0
3	31.982304693433242	33.0	31.0	34.0	30.0	34.0
4	35.7593792719356	37.0	35.0	37.0	35.0	37.0
5	32.0897707711002	37.0	35.0	37.0	10.0	37.0
6	33.96010645030631	37.0	35.0	37.0	19.0	37.0
7	20.850494911650042	32.0	0.0	37.0	0.0	37.0
8	28.177478355526304	35.0	17.0	37.0	17.0	37.0
9	34.91735545035694	37.0	32.0	39.0	32.0	39.0
10	36.253762467723156	37.0	35.0	39.0	32.0	39.0
11	36.590587185458965	38.0	35.0	39.0	32.0	39.0
12	36.89720773631715	39.0	35.0	39.0	33.0	39.0
13	36.61312401903701	39.0	35.0	39.0	33.0	39.0
14	37.937632904663054	40.0	37.0	41.0	33.0	41.0
15	38.1604855450357	40.0	37.0	41.0	33.0	41.0
16	38.24718368690193	40.0	37.0	41.0	34.0	41.0
17	38.24442749734191	40.0	37.0	41.0	34.0	41.0
18	38.14227127740367	40.0	37.0	41.0	34.0	41.0
19	38.0903087185459	40.0	37.0	41.0	34.0	41.0
20	38.069778112500636	40.0	37.0	41.0	34.0	41.0
21	37.967719988861326	40.0	36.0	41.0	33.0	41.0
22	37.95677117614298	40.0	36.0	41.0	33.0	41.0
23	37.92282985671611	40.0	36.0	41.0	33.0	41.0
24	37.784165991595366	40.0	36.0	41.0	33.0	41.0
25	37.64953673231735	39.0	36.0	41.0	33.0	41.0
26	37.51766049820262	39.0	35.0	41.0	33.0	41.0
27	37.48914928358058	39.0	35.0	41.0	33.0	41.0
28	37.401520809072956	39.0	35.0	41.0	33.0	41.0
29	37.409216621943195	39.0	35.0	41.0	33.0	41.0
30	37.2779859247633	39.0	35.0	41.0	32.0	41.0
31	37.13284453951699	39.0	35.0	41.0	32.0	41.0
32	37.03642853526404	39.0	35.0	41.0	32.0	41.0
33	36.904625082274315	39.0	35.0	41.0	31.0	41.0
34	36.739057516075135	39.0	35.0	41.0	31.0	41.0
35	36.612608222368486	39.0	35.0	41.0	31.0	41.0
36	36.47408042630753	39.0	35.0	41.0	30.0	41.0
37	36.37230709837477	39.0	35.0	40.0	30.0	41.0
38	36.24288010733634	39.0	35.0	40.0	30.0	41.0
39	36.21535934889373	39.0	35.0	40.0	30.0	41.0
40	36.12303490962483	39.0	35.0	40.0	30.0	41.0
41	36.017651005012404	39.0	35.0	40.0	29.0	41.0
42	35.91732380638955	39.0	35.0	40.0	29.0	41.0
43	35.82963204394714	38.0	35.0	40.0	28.0	41.0
44	35.699461419674954	38.0	35.0	40.0	28.0	41.0
45	35.56101273353248	38.0	35.0	40.0	27.0	41.0
46	35.450958179332694	38.0	34.0	40.0	27.0	41.0
47	35.44008214773935	38.0	34.0	40.0	27.0	41.0
48	35.30569338261354	38.0	34.0	40.0	26.0	41.0
49	35.20439787858842	38.0	34.0	40.0	26.0	41.0
50	35.03132436332338	38.0	34.0	40.0	26.0	41.0
51	33.13070857171789	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	11.0
10	6.0
11	16.0
12	5.0
13	2.0
14	7.0
15	8.0
16	18.0
17	57.0
18	110.0
19	181.0
20	329.0
21	591.0
22	881.0
23	1339.0
24	1981.0
25	2833.0
26	3519.0
27	4008.0
28	4442.0
29	5346.0
30	6492.0
31	8609.0
32	11501.0
33	16858.0
34	23922.0
35	29897.0
36	36123.0
37	59338.0
38	60242.0
39	37333.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.60807047744418	18.72088754999747	23.629183332489497	17.041858640068856
2	30.4823173510202	24.519961014632173	27.25653131487013	17.741190319477496
3	31.08513492987697	24.355095944509138	28.13053769429396	16.42923143131993
4	27.31159181813579	25.84299529137765	27.835615918181354	19.0097969723052
5	22.26089818237051	37.2386208293251	24.07947698850691	16.421003999797477
6	26.92047238114526	35.851032859095746	24.547491266265	12.681003493494
7	51.17462406966736	42.26241709280543	4.5681231330059235	1.9948357045212899
8	88.0468077565693	3.0947800111386767	6.484798238063895	2.37361399422814
9	82.44898992456078	6.161080451622702	7.964786593083895	3.425143030732621
10	42.93263632221153	31.457267986431063	13.932838843602854	11.677256847754544
11	31.347146979899755	25.698382360386816	26.27778340337198	16.676687256341452
12	28.75234165358716	23.4178016302972	29.81462963900562	18.01522707711002
13	23.521593843349702	26.925851855602247	31.323414004354213	18.22914029669384
14	20.209103336539922	29.061503215027084	30.571236899397498	20.158156549035493
15	19.437939851146776	25.449344336995594	36.00956913573996	19.103146676117667
16	22.606133866639663	24.432623664624575	33.99163333502101	18.969609133714748
17	23.300086071591313	23.072249506354108	29.51654346615361	24.111120955900965
18	24.228520074932916	23.71367272543162	31.530365551111334	20.527441648524125
19	25.064237253809935	25.374981013619564	28.66753582097109	20.89324591159941
20	26.5454913675257	25.331628778289705	28.908979292187738	19.213900561996862
21	24.532302161915855	26.743266163738543	29.308326160700727	19.41610551364488
22	23.80797174826591	23.63203128955496	29.099159536226015	23.460837425953116
23	21.882752772011543	26.913510708318565	29.23111488025923	21.972621639410665
24	21.985595666042226	24.77944154726343	32.64423320338211	20.590729583312235
25	21.470115437193055	26.095197711508277	30.9231178168194	21.511569034479265
26	20.76667004202319	27.961242468735758	29.129537744924306	22.142549744316746
27	21.31031340185307	27.740367576325248	29.68520581236393	21.26411320945775
28	19.552807452787203	27.1587514556225	31.999012708217307	21.289428383372993
29	20.28789681535112	25.047149511417143	31.896169814186624	22.76878385904511
30	21.933383119842034	25.849956964204345	30.578831451572075	21.63782846438155
31	24.482937572781125	25.930649081059187	28.827337856311075	20.759075489848616
32	24.7183686901929	26.237279125107587	28.454888360083036	20.589463824616473
33	23.985494405346564	25.543643359829883	28.01788517037112	22.452977064452433
34	21.14639765075186	25.611045010379218	30.583578046681183	22.658979292187738
35	21.358728671965977	26.635043795250873	29.13048706394613	22.875740468837023
36	24.367120652118878	26.428408688167686	27.547339375221508	21.657131284491925
37	21.95426813832211	28.14604323831705	29.49818996506506	20.401498658295782
38	20.869512936053873	28.771644473697535	28.228950432889473	22.129892157359123
39	21.786871550807554	26.59770391372589	28.796326768264898	22.81909776720166
40	23.381411067794037	25.555984507113568	28.569123082375576	22.493481342716827
41	19.98727912510759	25.397764670143285	29.089033466659913	25.525922738089214
42	21.707761632322413	25.373082375575922	28.030859197002684	24.88829679509898
43	21.753645385043797	24.93829426358159	28.947268492734544	24.36079185864007
44	21.57517340894132	25.74173459571667	29.443762341147284	23.239329654194723
45	20.8533745126829	27.230583261607006	28.15363779049162	23.76240443521847
46	21.742253556781936	26.989772669738244	28.18876259429902	23.0792111791808
47	20.480608576780924	25.97083691964964	29.961141208040097	23.58741329552934
48	21.874208900815148	24.896207786947496	30.42947192547213	22.800111386765227
49	21.584350159485595	25.006961672826694	30.597184952660623	22.811503215027088
50	20.287263936003242	26.837881626246773	29.64944812920865	23.22540630854134
51	19.928737785428584	26.21512834793175	28.62671510303276	25.229418763606905
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	174.0
1	411.5
2	649.0
3	1841.0
4	3033.0
5	2113.0
6	1193.0
7	1092.0
8	991.0
9	1036.0
10	1081.0
11	1085.5
12	1090.0
13	1092.0
14	1094.0
15	1062.0
16	1030.0
17	1004.0
18	978.0
19	1002.0
20	1026.0
21	1073.5
22	1121.0
23	1300.0
24	1479.0
25	1877.0
26	2950.5
27	3626.0
28	4318.0
29	5010.0
30	5524.0
31	6038.0
32	6883.5
33	7729.0
34	9024.0
35	10319.0
36	11195.5
37	12072.0
38	13115.0
39	14158.0
40	15817.5
41	17477.0
42	19114.0
43	20751.0
44	22295.0
45	23839.0
46	24806.0
47	25773.0
48	25408.0
49	25043.0
50	24188.5
51	23334.0
52	21753.0
53	20172.0
54	18600.5
55	17029.0
56	15863.0
57	14697.0
58	13875.5
59	13054.0
60	12124.0
61	11194.0
62	9974.5
63	8755.0
64	7488.5
65	6222.0
66	5230.5
67	4239.0
68	3644.5
69	3050.0
70	2565.0
71	2080.0
72	1643.5
73	1207.0
74	1031.0
75	676.5
76	498.0
77	405.0
78	312.0
79	226.5
80	141.0
81	104.5
82	68.0
83	48.0
84	28.0
85	22.5
86	17.0
87	12.5
88	8.0
89	6.5
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	316016.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.56818340673662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.05603459971205	29.121020949895165
2	8.664540764375722	7.203384392574318
3	4.51232837090958	5.627078799401715
4	3.2167674618667417	5.348607193267974
5	2.41490948803775	5.019170025471081
6	2.002399691593241	4.994167058024441
7	1.6516368990165828	4.805888187775853
8	1.3786020610866085	4.584478665612261
9	1.246012128017982	4.661501459802269
>10	4.836091720785295	27.46164818106057
>50	0.014550351013714512	0.43420546508112423
>100	0.004594847688541425	0.24352432609021993
>500	7.658079480902375E-4	0.17189952429897878
>1k	7.658079480902375E-4	0.32342577164400454
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1016	0.321502708723609	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	540	0.1708774239279024	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04177003696015392	0.0
2	0.0	0.0	0.0	0.19144600273403878	0.0
3	0.0	0.0	0.0	0.24777226469545846	0.0
4	0.0	0.0	0.0	0.32435066578907396	0.0
5	0.0	0.0	0.0	0.5756037668978786	0.0
6	0.0	0.0	0.0	0.7578730190876412	0.0
7	0.0	0.0	0.0	0.9689382816059946	0.0
8	0.0	0.0	0.0	1.4730266821933067	0.0
9	0.0	0.0	0.0	1.6290314414460028	0.0
10	0.0	0.0	0.0	2.10780466811807	0.0
11	0.0	0.0	0.0	2.700812617082679	0.0
12	0.0	0.0	0.0	3.182117361146271	0.0
13	0.0	0.0	0.0	3.371981165510607	0.0
14	0.0	0.0	0.0	3.4450787301908763	0.0
15	0.0	0.0	0.0	3.6118424383575514	0.0
16	0.0	0.0	0.0	3.9311300693635767	0.0
17	0.0	0.0	0.0	4.3368057313553745	0.0
18	0.0	0.0	0.0	4.759569135739962	0.0
19	0.0	0.0	0.0	5.010505797174827	0.0
20	0.0	0.0	0.0	5.2753658042630756	0.0
21	0.0	0.0	0.0	5.626297402663156	0.0
22	0.0	0.0	0.0	6.032605944002835	0.0
23	0.0	0.0	0.0	6.460748822844413	0.0
24	0.0	0.0	0.0	6.764847349501291	0.0
25	0.0	0.0	0.0	7.051541694091439	0.0
26	0.0	0.0	0.0	7.327160650093666	0.0
27	0.0	0.0	0.0	7.615753632727457	0.0
28	0.0	0.0	0.0	7.933142625689839	0.0
29	0.0	0.0	0.0	8.246101463217052	0.0
30	0.0	0.0	0.0	8.616652321401448	0.0
31	0.0	0.0	0.0	8.961888005670598	0.0
32	0.0	0.0	0.0	9.269467368740823	0.0
33	0.0	0.0	0.0	9.58938787909473	0.0
34	0.0	0.0	0.0	9.92323173510202	0.0
35	0.0	0.0	0.0	10.285555161763961	0.0
36	0.0	0.0	0.0	10.612120905270618	0.0
37	0.0	0.0	0.0	10.95672371019189	0.0
38	0.0	0.0	0.0	11.309237506961672	0.0
39	0.0	0.0	0.0	11.701622702647967	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	20	7.025516E-4	45.0	38
CGAACCA	20	7.025516E-4	45.0	15
TAACGCC	20	7.025516E-4	45.0	12
CTAACGG	20	7.025516E-4	45.0	2
ACTACTC	20	7.025516E-4	45.0	33
ACTAACG	20	7.025516E-4	45.0	1
CGTAAAG	20	7.025516E-4	45.0	2
CGATGCG	20	7.025516E-4	45.0	10
ATAACGC	20	7.025516E-4	45.0	11
TCGAACC	20	7.025516E-4	45.0	14
CGCCAGT	20	7.025516E-4	45.0	26
CGGAAAG	25	3.8843667E-5	44.999996	2
AGGCGAT	230	0.0	44.999996	7
CGTTAAG	25	3.8843667E-5	44.999996	2
GCGATCA	25	3.8843667E-5	44.999996	9
TAGGCGA	60	0.0	44.999996	6
GCTACGA	30	2.1604883E-6	44.999996	10
AATACGG	25	3.8843667E-5	44.999996	2
CGTTTTA	195	0.0	42.692307	1
GAGGCGA	65	0.0	41.53846	6
>>END_MODULE