FastQCFastQC Report
Sat 14 Jan 2017
SRR2934665.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934665.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476496
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28500.5981162486148887No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17590.3691531513381015No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6160.12927705584097243No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTA6450.042.9069751
CGTTATT2650.042.4528271
AGGCGAT3750.042.07
ACACGAC4650.041.12903226
AACACGT4500.041.00000441
CACGACC4700.040.6914927
CGACCAA4750.040.26315729
TAGGCGA950.040.2631576
TAATCGT1750.039.85714321
GCGAGAC4800.039.84375421
GACACGA4800.039.84375425
TACGGGA850.039.7058834
CACGTGA4650.039.6774243
TCAAGCG4850.039.4329917
ACGACCA4850.039.4329928
CGCATAG403.4525328E-739.3752
AAACACG4900.039.03061340
ACACGTG4750.038.84210642
ATACGAA2500.038.69999719
TCGTTGA1750.038.5714324
Produced by FastQC (version 0.11.5)