FastQCFastQC Report
Sat 14 Jan 2017
SRR2934663.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934663.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382703
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27870.7282409596998194No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16240.42434995283548865No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5770.1507696568879784No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGGC302.1616925E-645.0000044
ACAACGG253.885913E-545.02
AGGTACG207.027384E-445.07
GCGATAC253.885913E-545.09
CGTTTTA5550.044.189191
CGTTATT2550.044.117651
TAGGAGT603.6379788E-1241.2500046
ATCAACG556.002665E-1140.90909215
CGTTTTT10450.040.9090921
GTCAAGC5500.040.09090816
ATTCGGG451.9224899E-840.03
CGACCAA5300.039.90566329
ACGACCA5350.039.5327128
ACCGATA403.4501682E-739.37510
ACACGAC5500.039.27272826
ACAACGA1450.038.79310613
CACAACG1450.038.79310612
CGAATAT356.237371E-638.5714314
CACGACC5550.038.5135127
AACACGT4900.038.11224441
Produced by FastQC (version 0.11.5)