##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934663.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	382703
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.924777177079875	31.0	31.0	34.0	30.0	34.0
2	32.11416424747128	33.0	31.0	34.0	30.0	34.0
3	31.847942137898055	33.0	31.0	34.0	30.0	34.0
4	35.70255785818245	37.0	35.0	37.0	35.0	37.0
5	32.064483424483214	37.0	35.0	37.0	0.0	37.0
6	33.92880641123796	37.0	35.0	37.0	19.0	37.0
7	20.809894356720484	32.0	0.0	37.0	0.0	37.0
8	28.12462666872222	35.0	17.0	37.0	17.0	37.0
9	34.919875203486775	37.0	32.0	39.0	32.0	39.0
10	36.279752183808334	37.0	35.0	39.0	32.0	39.0
11	36.60692233925524	38.0	35.0	39.0	33.0	39.0
12	36.894787864218465	39.0	37.0	39.0	33.0	39.0
13	36.64884780103631	39.0	35.0	39.0	33.0	39.0
14	37.93224510913162	40.0	37.0	41.0	33.0	41.0
15	38.12466325061471	40.0	37.0	41.0	33.0	41.0
16	38.23119756051037	40.0	37.0	41.0	33.0	41.0
17	38.18923029085217	40.0	37.0	41.0	33.0	41.0
18	38.084553818496325	40.0	37.0	41.0	33.0	41.0
19	38.000483403579274	40.0	37.0	41.0	33.0	41.0
20	37.93919566870393	40.0	36.0	41.0	33.0	41.0
21	37.83219102019059	40.0	36.0	41.0	33.0	41.0
22	37.78164790973679	40.0	36.0	41.0	33.0	41.0
23	37.688055750804146	39.0	35.0	41.0	33.0	41.0
24	37.52155326715495	39.0	35.0	41.0	33.0	41.0
25	37.3616616540764	39.0	35.0	41.0	33.0	41.0
26	37.22064891051285	39.0	35.0	41.0	32.0	41.0
27	37.1777017687345	39.0	35.0	41.0	32.0	41.0
28	37.04557319906037	39.0	35.0	41.0	32.0	41.0
29	36.98672861200461	39.0	35.0	41.0	32.0	41.0
30	36.770725601837455	39.0	35.0	41.0	31.0	41.0
31	36.60434854181963	39.0	35.0	40.0	31.0	41.0
32	36.4518438580309	39.0	35.0	40.0	30.0	41.0
33	36.237202216862684	39.0	35.0	40.0	30.0	41.0
34	35.9937418833926	39.0	35.0	40.0	29.0	41.0
35	35.733608045925955	38.0	35.0	40.0	27.0	41.0
36	35.50950214657319	38.0	35.0	40.0	26.0	41.0
37	35.42417749534234	38.0	34.0	40.0	26.0	41.0
38	35.20210450401486	38.0	34.0	40.0	25.0	41.0
39	35.084726275989475	38.0	34.0	40.0	24.0	41.0
40	34.95057786325166	38.0	34.0	40.0	24.0	41.0
41	34.78539494072427	38.0	34.0	40.0	23.0	41.0
42	34.667593407942974	38.0	34.0	40.0	23.0	41.0
43	34.59454720762576	38.0	33.0	40.0	23.0	41.0
44	34.41818851694395	37.0	33.0	40.0	23.0	41.0
45	34.275984248882295	37.0	33.0	40.0	23.0	41.0
46	34.15539204030279	37.0	33.0	40.0	23.0	41.0
47	34.06262819993572	37.0	33.0	40.0	22.0	41.0
48	33.93045782238446	36.0	33.0	40.0	22.0	41.0
49	33.806682989158695	36.0	33.0	40.0	21.0	41.0
50	33.600094590322	36.0	33.0	40.0	20.0	41.0
51	31.79395249057363	35.0	29.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	13.0
10	18.0
11	7.0
12	6.0
13	10.0
14	20.0
15	26.0
16	58.0
17	109.0
18	183.0
19	326.0
20	634.0
21	1001.0
22	1622.0
23	2513.0
24	3796.0
25	5067.0
26	6334.0
27	6876.0
28	7159.0
29	7913.0
30	9345.0
31	11599.0
32	15255.0
33	21724.0
34	29870.0
35	36038.0
36	45612.0
37	68976.0
38	63038.0
39	37546.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44988150079827	16.844916292791016	20.981544435240902	18.723657771169812
2	34.571456194490246	22.853230834354576	25.148744587839655	17.426568383315523
3	32.47792674737329	22.708471059803557	28.631079453257485	16.18252273956567
4	27.36769766633656	25.879598539859895	28.681771504273552	18.07093228953
5	22.27105614536599	36.69712544714831	25.100142930679926	15.931675476805774
6	27.52865799327416	33.51580729704236	26.54852457388628	12.407010135797211
7	50.22981267458056	42.03050407234853	6.026605487806471	1.713077765264448
8	86.40329446071759	2.9166220280478594	8.859089163136948	1.820994348097611
9	81.74511305111274	4.76270110242146	10.393699552917015	3.0984862935487834
10	45.875260972608004	24.925072445212084	16.96824953031463	12.231417051865284
11	34.11418253841752	26.189238129829135	23.688343179959396	16.00823615179395
12	29.07920763620876	22.919078240829048	28.86468096670264	19.13703315625955
13	25.485820597173266	23.915412212603506	31.489693051792123	19.109074138431108
14	23.195794127561058	22.897651703801642	33.209041998625565	20.697512170011734
15	22.985709544999647	22.786076931719897	33.2187100702111	21.00950345306935
16	27.65852371159881	22.526084195838546	30.203316932451536	19.612075160111104
17	27.004753033030838	22.70716456364335	29.277011154864212	21.0110712484616
18	27.533100080218865	21.673203502454907	29.706325793108494	21.087370624217737
19	26.177740963619307	24.647050062319867	27.427796489706118	21.747412484354708
20	28.02382003799291	25.660891082641108	27.450268223661688	18.865020655704292
21	27.936023496026944	24.492360916951263	28.567845039103428	19.003770547918357
22	27.392521093380505	21.553528454179872	29.28981481723425	21.764135635205367
23	26.14481726038207	23.829183466029793	29.588218540225707	20.437780733362423
24	25.100665529144013	23.284113267991106	30.99008892012866	20.62513228273622
25	25.930290590875956	23.756542279522243	29.385450336161462	20.927716793440343
26	24.199967076296762	25.28174589694881	28.509836609590206	22.008450417164223
27	24.451598236752783	23.618053686540215	29.27727245409626	22.653075622610743
28	22.33794874876863	26.49887771979838	29.106121457109037	22.057052074323956
29	24.289070114422934	24.751308455904447	28.656425478765517	22.3031959509071
30	25.703482857463882	23.5318249399665	29.231545088488986	21.53314711408063
31	26.084979736244556	24.33218448770979	28.491545663347296	21.091290112698356
32	26.967125943616853	23.18743255213573	28.240175802123318	21.6052657021241
33	25.732225772988453	23.849303506896995	28.068763505904055	22.349707214210497
34	23.933441859614373	25.072445212083522	29.95064057506735	21.043472353234755
35	24.09361828885585	26.53519831305216	29.038444956010274	20.33273844208172
36	26.179831357475642	27.422831804297328	26.110064462520544	20.28727237570649
37	25.090474859094392	27.205169544006708	26.326158927418913	21.378196669479987
38	25.08368107906131	26.493913034389593	27.989328539363424	20.433077347185677
39	25.6859758089171	26.0355941813887	26.03193599214012	22.246494017554085
40	27.841955772491982	25.0144367825703	26.531801423035617	20.6118060219021
41	23.504388520602138	25.76488817699365	26.97548751904218	23.755235783362032
42	24.038222851663036	25.716809118298002	27.52081901631291	22.72414901372605
43	24.31310964377076	24.680235064789144	27.955620938430066	23.051034353010035
44	24.294557398295808	25.317282592506462	27.723848519609202	22.66431148958853
45	23.5117048990993	24.61935234372346	27.197591866277506	24.67135089089973
46	24.449769142128492	25.09413304834297	27.07269083336164	23.3834069761669
47	22.9810061588229	25.716809118298002	29.355400924476683	21.946783798402418
48	24.359882206306196	25.60079225927155	28.57751311068897	21.46181242373329
49	24.85399905409678	26.180615255171762	27.684392335570923	21.28099335516053
50	23.839635435311454	25.69642777819876	28.385980773602505	22.077956012887277
51	24.212509439434758	25.051541273520193	27.04081232705257	23.695136959992475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	244.0
1	603.5
2	963.0
3	4398.5
4	7834.0
5	5260.5
6	2687.0
7	2343.0
8	1999.0
9	1948.5
10	1898.0
11	1936.5
12	1975.0
13	1891.0
14	1807.0
15	1783.5
16	1760.0
17	1733.5
18	1707.0
19	1637.5
20	1568.0
21	1580.0
22	1592.0
23	1733.5
24	1875.0
25	2154.0
26	2836.5
27	3240.0
28	3791.0
29	4342.0
30	4918.0
31	5494.0
32	6357.5
33	7221.0
34	8250.5
35	9280.0
36	9984.5
37	10689.0
38	11532.5
39	12376.0
40	13682.5
41	14989.0
42	15853.0
43	16717.0
44	17762.5
45	18808.0
46	19862.0
47	20916.0
48	22182.5
49	23449.0
50	24126.5
51	24804.0
52	24774.5
53	24745.0
54	24567.5
55	24390.0
56	24087.5
57	23785.0
58	23115.5
59	22446.0
60	21211.0
61	19976.0
62	18355.0
63	16734.0
64	14931.5
65	13129.0
66	11680.5
67	10232.0
68	8983.5
69	7735.0
70	6621.0
71	5507.0
72	4833.0
73	4159.0
74	3614.0
75	2509.5
76	1950.0
77	1484.5
78	1019.0
79	810.5
80	602.0
81	446.5
82	291.0
83	206.5
84	122.0
85	99.5
86	77.0
87	49.0
88	21.0
89	21.5
90	22.0
91	17.5
92	13.0
93	12.0
94	11.0
95	6.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	382703.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.531816019784685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.27290886398916	35.18226978714616
2	12.0022783636635	12.369984009090993
3	6.844731364784881	10.581643121848325
4	4.438372343394552	9.148695481084346
5	2.898275536861257	7.467670087008848
6	2.015504414097969	6.231756159261627
7	1.2461253906501184	4.495057305799495
8	0.8066648332315179	3.32551230205735
9	0.5040663291019634	2.337790799974555
>10	0.948697356363999	6.370987311786277
>50	0.013851316675932791	0.49003982772998855
>100	0.006925658337964415	0.6292276107321522
>500	5.327429490741857E-4	0.1584050311485392
>1k	0.0010654858981483713	1.21096116533138
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2787	0.7282409596998194	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1624	0.42434995283548865	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	577	0.1507696568879784	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012542363137994738	0.0
2	0.0	0.0	0.0	0.04729516099952182	0.0
3	0.0	0.0	0.0	0.0838770534853398	0.0
4	0.0	0.0	0.0	0.11261996900991109	0.0
5	0.0	0.0	0.0	0.1842159585892977	0.0
6	0.0	0.0	0.0	0.30232321147208147	0.0
7	0.0	0.0	0.0	0.414681881249951	0.0
8	0.0	0.0	0.0	0.6916590672140015	0.0
9	0.0	0.0	0.0	0.8502677010632266	0.0
10	0.0	0.0	0.0	1.0893564983812511	0.0
11	0.0	0.0	0.0	1.3360229734284812	0.0
12	0.0	0.0	0.0	1.4909734180291243	0.0
13	0.0	0.0	0.0	1.5936640162214564	0.0
14	0.0	0.0	0.0	1.681721857419461	0.0
15	0.0	0.0	0.0	1.753056547766806	0.0
16	0.0	0.0	0.0	1.8541793505668887	0.0
17	0.0	0.0	0.0	1.9487696725659323	0.0
18	0.0	0.0	0.0	2.0849065724595834	0.0
19	0.0	0.0	0.0	2.1716579174973805	0.0
20	0.0	0.0	0.0	2.262851349479884	0.0
21	0.0	0.0	0.0	2.3765165154179613	0.0
22	0.0	0.0	0.0	2.4967141621570774	0.0
23	0.0	0.0	0.0	2.620308698912734	0.0
24	0.0	0.0	0.0	2.730054376370188	0.0
25	0.0	0.0	0.0	2.8411065499878494	0.0
26	0.0	0.0	0.0	2.9414454550918077	0.0
27	0.0	0.0	0.0	3.0634721964552147	0.0
28	0.0	0.0	0.0	3.1745243700728762	2.61299232041557E-4
29	0.0	0.0	0.0	3.3022996945411975	2.61299232041557E-4
30	0.0	0.0	0.0	3.5800607782013727	2.61299232041557E-4
31	0.0	0.0	0.0	3.764015437558629	2.61299232041557E-4
32	0.0	0.0	0.0	3.9837680917055787	2.61299232041557E-4
33	0.0	0.0	0.0	4.152306096372382	2.61299232041557E-4
34	0.0	0.0	0.0	4.308040438669151	2.61299232041557E-4
35	0.0	0.0	0.0	4.501140571147862	2.61299232041557E-4
36	0.0	0.0	0.0	4.657397511908712	2.61299232041557E-4
37	0.0	0.0	0.0	4.829071107360016	2.61299232041557E-4
38	0.0	0.0	0.0	5.02609072831935	2.61299232041557E-4
39	0.0	0.0	0.0	5.418562174845768	2.61299232041557E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGGC	30	2.1616925E-6	45.000004	4
ACAACGG	25	3.885913E-5	45.0	2
AGGTACG	20	7.027384E-4	45.0	7
GCGATAC	25	3.885913E-5	45.0	9
CGTTTTA	555	0.0	44.18919	1
CGTTATT	255	0.0	44.11765	1
TAGGAGT	60	3.6379788E-12	41.250004	6
ATCAACG	55	6.002665E-11	40.909092	15
CGTTTTT	1045	0.0	40.909092	1
GTCAAGC	550	0.0	40.090908	16
ATTCGGG	45	1.9224899E-8	40.0	3
CGACCAA	530	0.0	39.905663	29
ACGACCA	535	0.0	39.53271	28
ACCGATA	40	3.4501682E-7	39.375	10
ACACGAC	550	0.0	39.272728	26
ACAACGA	145	0.0	38.793106	13
CACAACG	145	0.0	38.793106	12
CGAATAT	35	6.237371E-6	38.57143	14
CACGACC	555	0.0	38.51351	27
AACACGT	490	0.0	38.112244	41
>>END_MODULE