##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934660.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	75094
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.561442991450715	31.0	31.0	34.0	30.0	34.0
2	31.722893972887313	31.0	31.0	34.0	30.0	34.0
3	31.060590726289718	31.0	31.0	34.0	27.0	34.0
4	35.217740431991906	37.0	35.0	37.0	33.0	37.0
5	31.701134577995578	35.0	35.0	37.0	0.0	37.0
6	33.68839055051003	37.0	35.0	37.0	17.0	37.0
7	21.34028018217168	32.0	0.0	37.0	0.0	37.0
8	28.354848589767492	35.0	17.0	37.0	17.0	37.0
9	34.68916291581218	37.0	32.0	39.0	29.0	39.0
10	36.09006045755986	37.0	35.0	39.0	32.0	39.0
11	36.52314432577836	38.0	35.0	39.0	32.0	39.0
12	36.98822808746371	39.0	37.0	39.0	33.0	39.0
13	36.808626521426476	39.0	37.0	39.0	33.0	39.0
14	38.184502090712975	40.0	37.0	41.0	33.0	41.0
15	38.31917330279383	40.0	38.0	41.0	34.0	41.0
16	38.33350201081311	40.0	38.0	41.0	34.0	41.0
17	38.26101952219884	40.0	37.0	41.0	34.0	41.0
18	38.09526726502783	40.0	37.0	41.0	34.0	41.0
19	37.945188696833306	40.0	37.0	41.0	34.0	41.0
20	37.77267158494686	39.0	35.0	41.0	34.0	41.0
21	37.63676192505393	39.0	35.0	41.0	33.0	41.0
22	37.49123764881349	39.0	35.0	40.0	33.0	41.0
23	37.39964577729246	39.0	35.0	40.0	33.0	41.0
24	37.153753961701334	39.0	35.0	40.0	33.0	41.0
25	36.996950488720806	39.0	35.0	40.0	33.0	41.0
26	36.816137108157776	38.0	35.0	40.0	32.0	41.0
27	36.75726422883319	38.0	35.0	40.0	32.0	41.0
28	36.6329134151863	38.0	35.0	40.0	32.0	41.0
29	36.532878791914136	38.0	35.0	40.0	31.0	41.0
30	36.22399925426799	38.0	35.0	40.0	31.0	41.0
31	35.94586784563347	38.0	35.0	40.0	30.0	41.0
32	35.60140623751565	38.0	35.0	40.0	27.0	41.0
33	35.21018989533118	38.0	34.0	40.0	25.0	41.0
34	34.77541481343383	38.0	34.0	40.0	22.0	41.0
35	34.37445068847045	38.0	33.0	40.0	20.0	41.0
36	34.065437984392894	38.0	33.0	40.0	18.0	41.0
37	33.864463206115005	38.0	33.0	40.0	15.0	41.0
38	33.66255626281727	38.0	33.0	40.0	15.0	41.0
39	33.53186672703545	38.0	33.0	40.0	14.0	41.0
40	33.46158148453938	38.0	33.0	40.0	13.0	41.0
41	33.30325991424082	38.0	33.0	40.0	12.0	41.0
42	33.2052094707966	38.0	33.0	40.0	12.0	41.0
43	33.04656830106267	38.0	32.0	40.0	10.0	41.0
44	32.92073934002717	37.0	32.0	40.0	10.0	41.0
45	32.830199483314246	37.0	31.0	40.0	10.0	41.0
46	32.681159613284684	37.0	31.0	40.0	10.0	41.0
47	32.58075212400458	37.0	31.0	40.0	10.0	41.0
48	32.43043385623352	37.0	31.0	40.0	10.0	41.0
49	32.29437771326604	36.0	31.0	40.0	10.0	41.0
50	32.033478040855464	36.0	30.0	40.0	10.0	41.0
51	30.322768796441792	35.0	26.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	3.0
11	1.0
12	1.0
13	2.0
14	3.0
15	8.0
16	6.0
17	16.0
18	44.0
19	83.0
20	193.0
21	318.0
22	469.0
23	858.0
24	1397.0
25	1845.0
26	2072.0
27	2046.0
28	2040.0
29	1888.0
30	1935.0
31	2372.0
32	2917.0
33	4047.0
34	5459.0
35	6532.0
36	7607.0
37	11878.0
38	11624.0
39	7426.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.834234426185844	14.55109595973047	18.777798492556	24.836871121527686
2	42.41217673848776	19.64737528963699	22.445202013476443	15.495245958398806
3	31.27946307294857	19.988281354036275	35.010786480943885	13.72146909207127
4	27.90902069406344	21.562308573254853	35.472873997922605	15.055796734759102
5	21.99243614669614	33.576584014701574	30.44717287666125	13.983806961941033
6	27.34306336058806	28.37243987535622	34.32231603057501	9.962180733480704
7	41.25629211388393	40.98729592244387	15.066450049271578	2.6899619144006177
8	67.82832183663142	5.720829893200522	22.39726209817029	4.053586171997763
9	60.03808559938211	8.940794204596905	25.444110048738917	5.577010147282073
10	32.740298825472074	24.739659626601327	31.063733454070896	11.4563080938557
11	24.234958851572696	24.268250459424188	36.908408128478975	14.588382560524144
12	23.12168748501878	21.21740751591339	40.01651263749434	15.644392361573495
13	22.04570271925853	23.269502223879403	40.085759181825445	14.59903587503662
14	17.812341864862706	23.314778810557435	41.7862945108797	17.086584813700163
15	18.71920526273737	24.059179162116813	42.42948837457054	14.792127200575278
16	22.069672676911605	22.752816470024236	39.8194263190135	15.358084534050656
17	19.676671904546303	23.633046581617705	38.87660798465923	17.813673529176764
18	19.73926012730711	22.904626201827043	41.004607558526644	16.3515061123392
19	18.327695954403815	25.0579273976616	38.443817082589824	18.170559565344767
20	19.471595600181107	24.547899965376725	39.82741630489786	16.153088129544305
21	20.52094707966016	24.475990092417504	39.9019895064852	15.101073321437134
22	19.243881002476897	21.591605188164166	40.646389858044586	18.51812395131435
23	18.560737209364262	22.37861879777346	41.213678855834026	17.84696513702826
24	19.387700748395346	21.791354835273125	41.753002903028204	17.067941513303325
25	17.274349481982583	23.831464564412602	41.27360374996671	17.620582203638108
26	17.274349481982583	24.502623378698697	41.01659253735318	17.206434601965537
27	17.283671132181	26.18584707167017	39.93128612139452	16.59919567475431
28	16.934775081897353	25.458758356193574	40.26287053559538	17.343596026313687
29	19.239886009534715	23.330758782326154	40.42133858896849	17.00801661917064
30	18.749833541960744	24.452020134764428	39.24814232828189	17.55000399499294
31	19.852451594002186	23.766213013023677	38.45846539004448	17.92287000292966
32	18.238474445361813	24.81156949956055	38.381228859829015	18.56872719524862
33	18.26910272458519	25.112525634538045	37.42908887527632	19.189282765600446
34	17.442139185554105	24.884811036833835	38.6955016379471	18.977548139664954
35	17.902895038218766	25.120515620422402	36.1933043918289	20.78328494952992
36	19.045463019682	26.29903853836525	34.79772019069433	19.857778251258424
37	18.998854768689906	26.92358910165925	35.809785069379714	18.267771060271127
38	17.9281966601859	27.741230990491918	35.285109329640186	19.045463019682
39	18.3050576610648	26.409566676432206	35.960263136868456	19.325112525634538
40	20.714038405198817	25.297626974192344	34.36359762431086	19.624736996297973
41	18.697898633712416	26.401576690547845	33.90151010733214	20.999014568407596
42	18.900311609449492	27.152635363677526	34.17316962740033	19.773883399472663
43	18.84571337257304	26.093962234000053	34.79505686206621	20.265267531360696
44	19.265187631501853	25.19375715769569	34.13987801954883	21.40117719125363
45	18.013423176285723	24.89945934428849	35.59405545050204	21.49306202892375
46	18.700561962340533	25.995419074759635	35.0201081311423	20.28391083175753
47	18.278424374783604	25.707779582922736	34.448824140410686	21.564971901882974
48	18.191866194369723	25.78102112019602	35.5061656057741	20.52094707966016
49	19.44229898527179	24.699709697179532	35.2944309798386	20.563560337710072
50	17.637893839720885	26.29770687405119	35.7804884544704	20.28391083175753
51	18.460862385809783	25.000665832157033	34.65789543771806	21.880576344315124
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	190.0
1	528.5
2	867.0
3	2333.5
4	3800.0
5	2583.0
6	1366.0
7	1207.0
8	1048.0
9	991.5
10	935.0
11	932.0
12	929.0
13	906.5
14	884.0
15	880.0
16	876.0
17	832.5
18	789.0
19	733.5
20	678.0
21	735.5
22	793.0
23	761.5
24	730.0
25	847.5
26	950.0
27	935.0
28	1123.5
29	1312.0
30	1505.5
31	1699.0
32	1871.5
33	2044.0
34	2266.5
35	2489.0
36	2597.0
37	2705.0
38	2876.0
39	3047.0
40	3333.5
41	3620.0
42	3762.0
43	3904.0
44	4175.0
45	4446.0
46	4813.5
47	5181.0
48	5288.5
49	5396.0
50	5308.5
51	5221.0
52	4705.0
53	4189.0
54	3780.5
55	3372.0
56	3095.5
57	2819.0
58	2522.0
59	2225.0
60	2063.5
61	1902.0
62	1673.0
63	1444.0
64	1134.0
65	824.0
66	675.5
67	527.0
68	434.0
69	341.0
70	283.5
71	226.0
72	190.5
73	155.0
74	122.5
75	78.5
76	67.0
77	48.5
78	30.0
79	21.0
80	12.0
81	10.5
82	9.0
83	7.5
84	6.0
85	4.0
86	2.0
87	1.0
88	0.0
89	1.0
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	75094.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.77526833035928
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3348521618543	42.66252962953099
2	7.738596404338567	8.322901962873198
3	3.55356346887227	5.73281487202706
4	2.2856718339854387	4.9165046475084555
5	1.639344262295082	4.407808879537646
6	1.196077460254569	3.859163182145045
7	0.8766282006834729	3.299864170239966
8	0.695854588678124	2.9935813780062324
9	0.5497498885642118	2.660665299491304
>10	2.0801347134862067	16.13178150051935
>50	0.03962161359021346	1.5114389964577728
>100	0.004952701698776683	0.6405305350627214
>500	0.0024763508493883413	1.0147282073135004
>1k	0.0024763508493883413	1.8456867392867604
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1386	1.8456867392867604	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	762	1.0147282073135004	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	313	0.4168109303006898	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	168	0.2237196047620316	No Hit
GGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA	97	0.12917143846379206	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	91	0.12118145257943379	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	87	0.11585479532319493	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82	0.10919647375289636	No Hit
GGAGGGGGGGGGCATCATCTGTCCTTATAGCTCATTAGGAAGAGAAACAGT	78	0.10386981649665751	No Hit
GGGGCTAGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA	76	0.1012064878685381	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0013316643140597118	0.0	0.0	0.078568194529523	0.0
2	0.0013316643140597118	0.0	0.0	0.3675393506804805	0.0
3	0.0013316643140597118	0.0	0.0	0.48872080325991424	0.0
4	0.0013316643140597118	0.0	0.0	0.6152289130955869	0.0
5	0.0013316643140597118	0.0	0.0	0.9681199563214105	0.0
6	0.0013316643140597118	0.0	0.0	1.2224678403068154	0.0
7	0.0013316643140597118	0.0	0.0	1.4967906890031162	0.0
8	0.0013316643140597118	0.0	0.0	2.157296188776733	0.0
9	0.0013316643140597118	0.0	0.0	2.3397341998029137	0.0
10	0.0013316643140597118	0.0	0.0	2.847098303459664	0.0
11	0.0013316643140597118	0.0	0.0	3.654086877779849	0.0
12	0.0013316643140597118	0.0	0.0	4.217380882627108	0.0
13	0.0013316643140597118	0.0	0.0	4.4477588089594375	0.0
14	0.0013316643140597118	0.0	0.0	4.536980318001438	0.0
15	0.0013316643140597118	0.0	0.0	4.772684901590007	0.0
16	0.0013316643140597118	0.0	0.0	5.282712333874877	0.0
17	0.0013316643140597118	0.0	0.0	5.888619596772045	0.0
18	0.0013316643140597118	0.0	0.0	6.505180174181692	0.0
19	0.0013316643140597118	0.0	0.0	6.906011132713665	0.0
20	0.0026633286281194236	0.0	0.0	7.3028470983034595	0.0
21	0.0026633286281194236	0.0	0.0	7.7556129650837615	0.0
22	0.0026633286281194236	0.0	0.0	8.384158521319947	0.0
23	0.0026633286281194236	0.0	0.0	8.932804218712548	0.0
24	0.0026633286281194236	0.0	0.0	9.358936799211655	0.0
25	0.0026633286281194236	0.0	0.0	9.803712680107598	0.0
26	0.0026633286281194236	0.0	0.0	10.16459370921778	0.0
27	0.0026633286281194236	0.0	0.0	10.546781367352917	0.0
28	0.0026633286281194236	0.0	0.0	10.983567262364502	0.0
29	0.0026633286281194236	0.0	0.0	11.381734892268357	0.0
30	0.0026633286281194236	0.0	0.0	11.897088981809466	0.0
31	0.0026633286281194236	0.0	0.0	12.39113644232562	0.0
32	0.0026633286281194236	0.0	0.0	12.895837217354249	0.0
33	0.0026633286281194236	0.0	0.0	13.367246384531388	0.0
34	0.0026633286281194236	0.0	0.0	13.761419021493062	0.0
35	0.0026633286281194236	0.0	0.0	14.219511545529603	0.0
36	0.0026633286281194236	0.0	0.0	14.65230244759901	0.0
37	0.0026633286281194236	0.0	0.0	15.142354915172984	0.0
38	0.0026633286281194236	0.0	0.0	15.604442432151703	0.0
39	0.0026633286281194236	0.0	0.0	16.055876634617945	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	25	3.855944E-5	45.0	18
CAGTTAT	20	6.9911755E-4	45.0	22
TATGCTT	20	6.9911755E-4	45.0	37
ATTGGGA	20	6.9911755E-4	45.0	4
TTACAGC	20	6.9911755E-4	45.0	16
GGCGATT	20	6.9911755E-4	45.0	8
AGGCGAT	35	1.1917473E-7	45.0	7
TAGTCTA	20	6.9911755E-4	45.0	25
GCTACGA	25	3.855944E-5	45.0	10
AGGTGAG	20	6.9911755E-4	45.0	7
GTTTAGG	25	3.855944E-5	45.0	3
TAACTAG	20	6.9911755E-4	45.0	30
CTAACTA	20	6.9911755E-4	45.0	29
TCATGTT	20	6.9911755E-4	45.0	16
TTCTAGT	20	6.9911755E-4	45.0	22
CTAGCCA	20	6.9911755E-4	45.0	33
ATGCATT	20	6.9911755E-4	45.0	28
CTACGAA	25	3.855944E-5	45.0	11
CGGCTAC	20	6.9911755E-4	45.0	25
GCCAAGC	20	6.9911755E-4	45.0	19
>>END_MODULE