##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934657.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	132562
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.811152517312653	31.0	31.0	34.0	30.0	34.0
2	31.968776874217347	33.0	31.0	34.0	30.0	34.0
3	31.691766871350765	33.0	31.0	34.0	30.0	34.0
4	35.60942804121845	37.0	35.0	37.0	33.0	37.0
5	32.00365112173926	37.0	35.0	37.0	0.0	37.0
6	33.8843484558169	37.0	35.0	37.0	19.0	37.0
7	20.87608817006382	32.0	0.0	37.0	0.0	37.0
8	28.157194369427135	35.0	17.0	37.0	17.0	37.0
9	34.818507566270874	37.0	32.0	39.0	32.0	39.0
10	36.130542689458515	37.0	35.0	39.0	32.0	39.0
11	36.50852431315158	38.0	35.0	39.0	32.0	39.0
12	36.938051628671865	39.0	37.0	39.0	33.0	39.0
13	36.75745688809764	39.0	35.0	39.0	33.0	39.0
14	38.04664986949503	40.0	37.0	41.0	33.0	41.0
15	38.21402060922436	40.0	37.0	41.0	33.0	41.0
16	38.3272355577013	40.0	38.0	41.0	34.0	41.0
17	38.26926268463059	40.0	37.0	41.0	34.0	41.0
18	38.11151762948658	40.0	37.0	41.0	33.0	41.0
19	38.02899020835534	40.0	37.0	41.0	34.0	41.0
20	37.96311160060952	40.0	36.0	41.0	34.0	41.0
21	37.857478010289526	40.0	36.0	41.0	33.0	41.0
22	37.80074229417178	39.0	36.0	41.0	33.0	41.0
23	37.703414251444606	39.0	35.0	41.0	33.0	41.0
24	37.549026116081535	39.0	35.0	41.0	33.0	41.0
25	37.36649266003832	39.0	35.0	41.0	33.0	41.0
26	37.203338815045036	39.0	35.0	41.0	33.0	41.0
27	37.1855282811062	39.0	35.0	41.0	33.0	41.0
28	37.05846320966793	39.0	35.0	41.0	32.0	41.0
29	36.952429806430196	39.0	35.0	41.0	32.0	41.0
30	36.722047042138776	39.0	35.0	41.0	31.0	41.0
31	36.52704394924639	39.0	35.0	40.0	30.0	41.0
32	36.29603506283852	39.0	35.0	40.0	30.0	41.0
33	36.03546265143857	39.0	35.0	40.0	29.0	41.0
34	35.74588494440337	39.0	35.0	40.0	27.0	41.0
35	35.514612030597	39.0	35.0	40.0	25.0	41.0
36	35.28310526395196	39.0	35.0	40.0	24.0	41.0
37	35.143630904784175	38.0	34.0	40.0	24.0	41.0
38	34.94877114105098	38.0	34.0	40.0	23.0	41.0
39	34.83777402272144	38.0	34.0	40.0	22.0	41.0
40	34.73803201520798	38.0	34.0	40.0	22.0	41.0
41	34.60233701965873	38.0	34.0	40.0	21.0	41.0
42	34.449631116006096	38.0	34.0	40.0	20.0	41.0
43	34.34772408382493	38.0	33.0	40.0	20.0	41.0
44	34.202297792730946	38.0	33.0	40.0	18.0	41.0
45	34.139293311808814	38.0	33.0	40.0	20.0	41.0
46	34.02533908661607	38.0	33.0	40.0	19.0	41.0
47	33.98648934083674	38.0	33.0	40.0	19.0	41.0
48	33.79978425189722	38.0	33.0	40.0	18.0	41.0
49	33.704425099198865	37.0	33.0	40.0	18.0	41.0
50	33.46607625111269	37.0	33.0	40.0	17.0	41.0
51	31.62809100647244	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	4.0
10	3.0
11	5.0
12	1.0
13	0.0
14	2.0
15	4.0
16	7.0
17	30.0
18	63.0
19	113.0
20	234.0
21	412.0
22	699.0
23	1084.0
24	1606.0
25	2173.0
26	2592.0
27	2743.0
28	2792.0
29	2825.0
30	3146.0
31	3882.0
32	4895.0
33	6990.0
34	9456.0
35	11664.0
36	14381.0
37	23266.0
38	23078.0
39	14407.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.45833647651665	16.91057769194792	20.51266577148806	21.118420060047374
2	33.79852446402438	22.48985380425763	25.13993452120517	18.571687210512817
3	31.12430409921395	22.443083236523286	30.613599674114756	15.819012990148007
4	28.63490291335375	23.920127940133675	29.77172945489658	17.673239691616
5	22.07872542659284	34.472925876193784	27.502602555785217	15.945746141428163
6	27.889591285587123	32.997389900574824	28.341455319020536	10.771563494817519
7	47.352936739035314	41.87172794616858	8.92940661728097	1.845928697515125
8	78.77974080053107	3.8057663583832473	13.893876073082783	3.520616768002897
9	73.30758437561292	6.157118933027564	16.35083960712723	4.184457084232284
10	36.72771985938655	26.664504156545615	23.81979752870355	12.787978455364282
11	25.560115266818546	25.696655150042996	32.08310073776799	16.660128845370465
12	25.820370845340296	21.285134503100437	34.50687225600097	18.387622395558303
13	22.622621867503508	24.034791267482387	35.30725245545481	18.0353344095593
14	19.58781551274121	23.44336989484166	36.406360797211875	20.56245379520526
15	18.648632338075767	26.15983464341214	36.958555242075406	18.232977776436687
16	23.295514551681475	25.044884657745055	33.9833436429746	17.676257147598857
17	22.836861242286627	25.247808572592444	32.6141729907515	19.301157194369427
18	21.57631900544651	23.723993301247717	34.86142333398712	19.838264359318657
19	20.50285903954376	25.803020473438842	33.89810051145879	19.796019975558607
20	21.12370060801738	27.2189616933963	34.93610536956292	16.7212323290234
21	22.20168675789442	25.157284893106624	34.96175374541724	17.67927460358172
22	21.434498574252046	22.532852552013395	35.37967139904347	20.652977474691088
23	20.751044794134067	23.587453418023266	35.126959460478865	20.5345423273638
24	20.174710701407644	23.727765121226295	36.74733332327514	19.350190854090915
25	20.345951328434996	25.152004345136614	35.346479383232	19.15556494319639
26	18.638071242135755	27.04621233837751	34.31450943709359	20.001206982393143
27	19.452784357508186	24.89778367858059	34.295650337200705	21.353781626710518
28	17.36244172538133	26.687135076417075	35.08697816870596	20.86344502949563
29	18.661456526002926	24.494953304868666	34.80710912629562	22.036481042832786
30	20.300689488692083	25.471854679319865	33.39041354234245	20.8370422896456
31	20.204885261236253	25.167845989046633	33.26066293507943	21.36660581463768
32	21.41035892638916	25.690620238077276	31.79568805540049	21.10333278013307
33	21.25420557927611	24.7544545193947	31.83717807516483	22.154161826164362
34	18.554336838611366	24.89250313061058	34.44652313634375	22.106636894434303
35	19.346419034112337	25.591798554638583	33.23350583123369	21.828276580015388
36	21.993482295077023	26.159080279416425	30.21755857636427	21.62987884914229
37	20.398002444139347	25.9395603566633	32.02803216608078	21.63440503311658
38	20.31049622063638	27.805856882062734	30.689790437681992	21.193856459618896
39	20.86872557746564	27.014529050557474	30.782577209154958	21.334168162821925
40	22.744074470813658	25.64309530634722	31.372489853804257	20.240340369034868
41	20.24637528100059	24.970956986164964	31.782109503477617	23.00055822935683
42	20.820446281739866	25.114286145350857	31.931473574629234	22.133793998280048
43	20.416107180036512	25.177652720990935	31.271405078378418	23.13483502059414
44	19.995172070427422	27.125420557927608	30.14287654078846	22.736530830856506
45	20.106817941793274	24.500988216834386	31.042832787676712	24.349361053695628
46	20.706537318386868	25.39868137173549	31.220862690665502	22.67391861921214
47	20.098519937840408	25.1512499811409	32.96268915677192	21.787540924246766
48	20.79479790588555	24.173594242693987	32.312427392465416	22.719180458955055
49	21.3379399828005	25.587272370664294	31.428312789487183	21.646474857048023
50	21.266275403207555	25.07128739759509	31.120532279235373	22.54190491996198
51	20.475701935698012	24.053650367375266	31.091112083402482	24.379535613524236
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	241.0
1	634.0
2	1027.0
3	2023.5
4	3020.0
5	2116.0
6	1212.0
7	1149.5
8	1087.0
9	1059.0
10	1031.0
11	1039.0
12	1047.0
13	1057.5
14	1068.0
15	1035.0
16	1002.0
17	949.5
18	897.0
19	899.0
20	901.0
21	856.0
22	811.0
23	886.0
24	961.0
25	1119.0
26	1467.0
27	1657.0
28	1863.0
29	2069.0
30	2411.5
31	2754.0
32	3216.5
33	3679.0
34	3971.5
35	4264.0
36	4488.0
37	4712.0
38	5177.5
39	5643.0
40	6171.0
41	6699.0
42	7004.0
43	7309.0
44	7560.5
45	7812.0
46	8118.5
47	8425.0
48	8923.0
49	9421.0
50	9768.0
51	10115.0
52	9846.0
53	9577.0
54	8458.5
55	7340.0
56	6714.0
57	6088.0
58	5703.5
59	5319.0
60	4784.5
61	4250.0
62	3689.0
63	3128.0
64	2667.0
65	2206.0
66	1846.5
67	1487.0
68	1295.5
69	1104.0
70	948.0
71	792.0
72	602.5
73	413.0
74	359.0
75	247.5
76	190.0
77	135.5
78	81.0
79	75.0
80	69.0
81	56.0
82	43.0
83	28.5
84	14.0
85	10.0
86	6.0
87	4.5
88	3.0
89	3.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	132562.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.911452754182946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.78851907831827	36.09103664700291
2	8.75566797248527	8.565048807350523
3	4.523581850149603	6.637648798298155
4	2.9334649433973903	5.739201279401337
5	2.284154353928252	5.586065388271148
6	1.7736512538943212	5.205111570434966
7	1.347974952959684	4.61519892578567
8	1.0657330577747617	4.170124168313695
9	0.8328449366112465	3.6662090191759327
>10	2.655849964526975	17.034293387245214
>50	0.026219192448872573	0.8999562468882486
>100	0.00925383262901385	0.6027368325764548
>500	0.00308461087633795	1.1873689292557446
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	992	0.7483290837494908	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	582	0.43903984550625363	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	215	0.16218825907877069	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0784538555543821	0.0
2	0.0	0.0	0.0	0.34776180202471296	0.0
3	0.0	0.0	0.0	0.44356602948054497	0.0
4	0.0	0.0	0.0	0.5672817247778398	0.0
5	0.0	0.0	0.0	0.909008614836831	0.0
6	0.0	0.0	0.0	1.125511081607097	0.0
7	0.0	0.0	0.0	1.342013548377363	0.0
8	0.0	0.0	0.0	1.941732924970957	0.0
9	0.0	0.0	0.0	2.1310782878954755	0.0
10	0.0	0.0	0.0	2.687798916733302	0.0
11	0.0	0.0	0.0	3.4881791161871427	0.0
12	0.0	0.0	0.0	4.024531917140659	0.0
13	0.0	0.0	0.0	4.268945851752387	0.0
14	0.0	0.0	0.0	4.360223895233928	0.0
15	7.543639957152125E-4	0.0	0.0	4.569937086042757	0.0
16	7.543639957152125E-4	0.0	0.0	5.009731295544726	0.0
17	7.543639957152125E-4	0.0	0.0	5.542312276519667	0.0
18	7.543639957152125E-4	0.0	0.0	6.068103981533169	0.0
19	7.543639957152125E-4	0.0	0.0	6.396252319669287	0.0
20	7.543639957152125E-4	0.0	0.0	6.740996665711139	0.0
21	7.543639957152125E-4	0.0	0.0	7.164949231303089	0.0
22	7.543639957152125E-4	0.0	0.0	7.628128724672229	0.0
23	7.543639957152125E-4	0.0	0.0	8.14185060575429	0.0
24	7.543639957152125E-4	0.0	0.0	8.517523875620464	0.0
25	7.543639957152125E-4	0.0	0.0	8.915073701362381	0.0
26	7.543639957152125E-4	0.0	0.0	9.220591119627043	0.0
27	7.543639957152125E-4	0.0	0.0	9.582685837570345	0.0
28	7.543639957152125E-4	0.0	0.0	9.92365836363362	0.0
29	7.543639957152125E-4	0.0	0.0	10.294805449525505	0.0
30	7.543639957152125E-4	0.0	0.0	10.7904225947104	0.0
31	7.543639957152125E-4	0.0	0.0	11.139693124726543	0.0
32	7.543639957152125E-4	0.0	0.0	11.50103347867413	0.0
33	7.543639957152125E-4	0.0	0.0	11.861619468626001	0.0
34	7.543639957152125E-4	0.0	0.0	12.177697982830676	0.0
35	7.543639957152125E-4	0.0	0.0	12.544318884748268	0.0
36	7.543639957152125E-4	0.0	0.0	12.873221586880101	0.0
37	7.543639957152125E-4	0.0	0.0	13.208159200977656	0.0
38	7.543639957152125E-4	0.0	0.0	13.537061903109489	0.0
39	7.543639957152125E-4	0.0	0.0	13.983645388572894	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCCC	20	7.010697E-4	45.000004	16
GCACGGG	20	7.010697E-4	45.000004	3
CGTCTAC	20	7.010697E-4	45.000004	35
TGACGTT	20	7.010697E-4	45.000004	28
CCGTCTA	20	7.010697E-4	45.000004	34
CATTAAG	20	7.010697E-4	45.000004	22
CGGGGAT	20	7.010697E-4	45.000004	5
TAATTGT	20	7.010697E-4	45.000004	24
GTGTACG	20	7.010697E-4	45.000004	1
TCATCCA	20	7.010697E-4	45.000004	41
GGACCCC	45	3.8016879E-10	45.000004	8
AGGATGC	20	7.010697E-4	45.000004	7
CGTTTTA	140	0.0	45.000004	1
TATCGGG	20	7.010697E-4	45.000004	3
GCACTAG	20	7.010697E-4	45.000004	2
GCGTAAA	20	7.010697E-4	45.000004	1
ACTTATA	25	3.872095E-5	45.0	7
TACGAAT	25	3.872095E-5	45.0	12
ACAACGA	30	2.1509459E-6	44.999996	13
CAACGAG	30	2.1509459E-6	44.999996	14
>>END_MODULE