##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934656.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	364065
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07428343839699	33.0	31.0	34.0	30.0	34.0
2	32.26986115116806	33.0	31.0	34.0	30.0	34.0
3	32.153283067584084	33.0	31.0	34.0	30.0	34.0
4	35.90041064095697	37.0	35.0	37.0	35.0	37.0
5	32.14583384835126	37.0	35.0	37.0	0.0	37.0
6	34.02980786398033	37.0	35.0	37.0	19.0	37.0
7	20.666045898397265	32.0	0.0	37.0	0.0	37.0
8	28.07953250106437	35.0	17.0	37.0	17.0	37.0
9	34.9579031216953	37.0	32.0	39.0	32.0	39.0
10	36.316871987145156	37.0	35.0	39.0	32.0	39.0
11	36.708889896035046	38.0	35.0	39.0	33.0	39.0
12	37.06043975663687	39.0	37.0	39.0	34.0	39.0
13	36.88366088473212	39.0	37.0	39.0	33.0	39.0
14	38.26384848859407	40.0	38.0	41.0	34.0	41.0
15	38.43911664125912	40.0	38.0	41.0	34.0	41.0
16	38.501654924258034	40.0	38.0	41.0	34.0	41.0
17	38.471333415736204	40.0	38.0	41.0	34.0	41.0
18	38.395794706989136	40.0	38.0	41.0	34.0	41.0
19	38.338120939942044	40.0	37.0	41.0	34.0	41.0
20	38.3068270775823	40.0	37.0	41.0	34.0	41.0
21	38.209577959979676	40.0	37.0	41.0	34.0	41.0
22	38.190597832804585	40.0	37.0	41.0	34.0	41.0
23	38.10846689464793	40.0	37.0	41.0	34.0	41.0
24	37.9572466455166	40.0	36.0	41.0	33.0	41.0
25	37.78963371925343	40.0	36.0	41.0	33.0	41.0
26	37.66512847980443	40.0	36.0	41.0	33.0	41.0
27	37.640794912996306	40.0	36.0	41.0	33.0	41.0
28	37.56773103704009	40.0	36.0	41.0	33.0	41.0
29	37.51366376883249	40.0	36.0	41.0	33.0	41.0
30	37.326070893933775	40.0	36.0	41.0	32.0	41.0
31	37.195709557359265	39.0	35.0	41.0	32.0	41.0
32	37.07900512271161	39.0	35.0	41.0	32.0	41.0
33	36.94971227665389	39.0	35.0	41.0	31.0	41.0
34	36.764363506516695	39.0	35.0	41.0	31.0	41.0
35	36.57676788485573	39.0	35.0	41.0	30.0	41.0
36	36.381486822408085	39.0	35.0	41.0	30.0	41.0
37	36.334173842583056	39.0	35.0	41.0	30.0	41.0
38	36.17555381593946	39.0	35.0	41.0	29.0	41.0
39	36.08210072377185	39.0	35.0	41.0	29.0	41.0
40	35.99068572919671	39.0	35.0	40.0	28.0	41.0
41	35.85427876890116	39.0	35.0	40.0	28.0	41.0
42	35.73063875956217	39.0	35.0	40.0	27.0	41.0
43	35.66424951588315	38.0	35.0	40.0	27.0	41.0
44	35.468180132668614	38.0	35.0	40.0	27.0	41.0
45	35.35065441610701	38.0	34.0	40.0	26.0	41.0
46	35.23458997706454	38.0	34.0	40.0	26.0	41.0
47	35.13544559350665	38.0	34.0	40.0	26.0	41.0
48	35.01656572315383	38.0	34.0	40.0	25.0	41.0
49	34.89696894785272	38.0	34.0	40.0	25.0	41.0
50	34.70500322744565	37.0	34.0	40.0	24.0	41.0
51	32.86691112850727	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	8.0
10	10.0
11	15.0
12	4.0
13	16.0
14	15.0
15	26.0
16	39.0
17	82.0
18	135.0
19	230.0
20	404.0
21	721.0
22	1114.0
23	1781.0
24	2516.0
25	3527.0
26	4288.0
27	4743.0
28	5099.0
29	6090.0
30	7219.0
31	9264.0
32	12407.0
33	18246.0
34	25380.0
35	32644.0
36	42925.0
37	69493.0
38	71120.0
39	44494.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.364234408690756	17.08074107645613	22.55393954376279	17.00108497109033
2	31.889360416409158	23.791905291637484	26.237347726367545	18.081386565585817
3	32.3101643937209	23.45817367777732	27.077307623638635	17.154354304863144
4	27.86013486602667	26.593602790710452	27.02072432120638	18.5255380220565
5	22.208671528435854	38.09759246288438	22.642934640792166	17.0508013678876
6	28.01642563827888	35.35934517187865	24.20721574444124	12.417013445401233
7	50.92964168486397	42.77368052408224	4.371197451004629	1.9254803400491671
8	88.10487138285745	3.2854023320011536	6.572452721354703	2.037273563786686
9	83.26040679548981	5.297405683051103	8.033455564253636	3.408731957205444
10	44.26709516157829	27.27095436254515	15.495859255902106	12.966091219974455
11	30.25943169488965	26.665293285539672	24.21902682213341	18.85624819743727
12	26.636452281872742	22.56575062145496	30.300633128699545	20.49716396797275
13	23.303256286652108	25.882466043151638	30.351173554173016	20.46310411602324
14	21.189897408429815	26.520264238528835	30.745333937621027	21.544504415420324
15	20.321920536167994	23.799046873497865	32.691140318349746	23.1878922719844
16	24.39509428261437	22.11638031670169	30.462417425459737	23.026107975224207
17	24.128933020202435	23.52574402922555	29.256314119731368	23.089008830840648
18	24.954609753752763	23.519426476041367	29.616689327455266	21.909274442750608
19	24.9002238611237	25.77094749563951	26.48647906280472	22.842349580432067
20	28.023567220139263	25.9363025833299	26.299973905758588	19.74015629077225
21	27.045994533943112	24.59423454602887	27.21821652726848	21.141554392759534
22	26.7350610467911	22.36551165313886	26.690838174501806	24.208589125568235
23	26.03875681540384	24.080040652081358	27.685166110447312	22.19603642206749
24	24.27203933363548	24.25830552236551	30.29486492796616	21.17479021603285
25	22.958537623775975	26.15604356364935	29.066787524205846	21.818631288368838
26	22.977764959553927	25.764080590004536	28.059824481891972	23.198329968549572
27	22.109513411066708	25.232856770082268	28.66054138684026	23.99708843201077
28	21.50962053479461	25.616854133190504	29.48978891132078	23.383736420694106
29	21.983162347383022	25.061733481658493	28.05707771963798	24.898026451320508
30	23.531237553733536	25.86845755565627	28.603958084408003	21.996346806202187
31	25.60119758834274	26.187356653344867	26.661997170834876	21.54944858747751
32	24.00780080480134	26.34227404447008	26.888879733014708	22.76104541771387
33	24.19046049469188	25.347396756073774	26.75593643992144	23.706206309312897
34	23.0263826514496	24.809855382967328	29.009380193097385	23.15438177248568
35	24.544518149231592	27.136912364550287	27.55881504676363	20.759754439454493
36	25.691566066499117	28.41003666927609	24.94499608586379	20.953401178361005
37	24.417892409322512	27.047367915070108	26.3351324626097	22.19960721299768
38	22.961833738480767	27.484377789680416	27.66923488937415	21.88455358246467
39	22.85361130567344	28.24275884800791	26.07473940093115	22.8288904453875
40	24.86369192314559	25.058712043179103	27.070990070454453	23.006605963220853
41	21.97986623267823	25.933555821075906	27.18662876134756	24.8999491848983
42	22.379520140634227	27.206954802027113	27.20777883070331	23.205746226635355
43	22.36386359578647	24.380811118893604	29.01157760290058	24.243747682419347
44	22.61684039937923	25.371293587683518	28.241385466880914	23.770480546056337
45	23.457349649101122	26.238995783719943	26.6518341504951	23.651820416683833
46	23.161248678120668	25.015313199566013	27.138011069451885	24.68542705286144
47	21.731009572466455	26.455165973109196	28.205952233804403	23.607872220619942
48	22.55421421998819	27.136912364550287	28.612747723620778	21.69612569184074
49	23.961655198934256	25.75007210250917	28.61439578097318	21.6738769175834
50	23.999835194264758	26.04946918819442	27.99967038852952	21.951025229011304
51	23.487014681444247	25.479790696716247	26.921566203837227	24.11162841800228
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	211.0
1	668.0
2	1125.0
3	2133.5
4	3142.0
5	2253.0
6	1364.0
7	1261.0
8	1158.0
9	1168.5
10	1179.0
11	1198.5
12	1218.0
13	1270.5
14	1323.0
15	1288.5
16	1254.0
17	1253.5
18	1253.0
19	1289.0
20	1325.0
21	1432.5
22	1540.0
23	1682.0
24	1824.0
25	2172.5
26	3097.0
27	3673.0
28	4390.5
29	5108.0
30	5965.0
31	6822.0
32	7687.0
33	8552.0
34	9618.0
35	10684.0
36	11601.0
37	12518.0
38	13496.5
39	14475.0
40	15377.0
41	16279.0
42	17675.0
43	19071.0
44	20371.5
45	21672.0
46	22085.5
47	22499.0
48	22692.0
49	22885.0
50	22584.0
51	22283.0
52	21994.0
53	21705.0
54	21597.5
55	21490.0
56	21997.0
57	22504.0
58	21914.0
59	21324.0
60	19509.5
61	17695.0
62	16029.0
63	14363.0
64	12818.5
65	11274.0
66	9785.5
67	8297.0
68	7122.0
69	5947.0
70	5060.0
71	4173.0
72	3648.0
73	3123.0
74	2650.5
75	1823.0
76	1468.0
77	1079.5
78	691.0
79	543.0
80	395.0
81	310.0
82	225.0
83	164.5
84	104.0
85	74.0
86	44.0
87	45.5
88	47.0
89	41.0
90	35.0
91	27.0
92	19.0
93	11.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	364065.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.36842977661053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.51883892543773	27.104114871320274
2	9.926235123627453	8.212655213158145
3	5.919902535865718	7.346912170180186
4	4.261312537369658	7.051352338334669
5	3.2482015167004206	6.7186498171950575
6	2.4803807175353185	6.156567331957121
7	2.01465662824858	5.834022687477902
8	1.5588139637411462	5.158854879506043
9	1.2377273047114834	4.608255157879538
>10	3.813049498592649	20.65192673105925
>50	0.013471773012829284	0.3686579824032305
>100	0.0060622978557731775	0.347759003808943
>500	0.0013471773012829283	0.44027181571965546
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	977	0.26835867221512644	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	603	0.16562976391578427	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.021699421806545535	0.0
2	0.0	0.0	0.0	0.0727891997308173	0.0
3	0.0	0.0	0.0	0.09448862153736283	0.0
4	0.0	0.0	0.0	0.12690041613448147	0.0
5	0.0	0.0	0.0	0.22303709502424018	0.0
6	0.0	0.0	0.0	0.32576600332358235	0.0
7	0.0	0.0	0.0	0.4273962067213272	0.0
8	0.0	0.0	0.0	0.6800983340886929	0.0
9	0.0	0.0	0.0	0.8234793237471331	0.0
10	0.0	0.0	0.0	1.0528339719555573	0.0
11	0.0	0.0	0.0	1.3978273110570913	0.0
12	0.0	0.0	0.0	1.646134618818068	0.0
13	0.0	0.0	0.0	1.7675415104445635	0.0
14	0.0	0.0	0.0	1.8235754604260228	0.0
15	0.0	0.0	0.0	1.918338758188785	0.0
16	0.0	0.0	0.0	2.086440608133163	0.0
17	0.0	0.0	0.0	2.2833834617444686	0.0
18	0.0	0.0	0.0	2.493236097949542	0.0
19	0.0	0.0	0.0	2.6300248581983987	0.0
20	0.0	0.0	0.0	2.7841182206474118	0.0
21	0.0	0.0	0.0	2.96403114828396	0.0
22	0.0	0.0	0.0	3.16729155507945	0.0
23	0.0	0.0	0.0	3.4054358425006526	0.0
24	0.0	0.0	0.0	3.5952371142515758	0.0
25	0.0	0.0	0.0	3.7674591075769435	0.0
26	0.0	0.0	0.0	3.9124881545877797	0.0
27	0.0	0.0	0.0	4.0764698611511685	0.0
28	0.0	0.0	0.0	4.235507395657369	0.0
29	0.0	0.0	0.0	4.41404694216692	0.0
30	0.0	0.0	0.0	4.693392663398019	0.0
31	0.0	0.0	0.0	4.901047889799899	0.0
32	0.0	0.0	0.0	5.109252468652576	0.0
33	0.0	0.0	0.0	5.303723236235288	0.0
34	0.0	0.0	0.0	5.52978176973892	0.0
35	0.0	0.0	0.0	5.76875008583632	0.0
36	0.0	0.0	0.0	5.964594234546029	0.0
37	0.0	0.0	0.0	6.177468309230495	0.0
38	0.0	0.0	0.0	6.399956051803936	0.0
39	0.0	0.0	0.0	6.714735006111546	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGCG	35	1.208864E-7	45.0	5
CGCGGAT	20	7.0269307E-4	45.0	31
ATTCGTC	20	7.0269307E-4	45.0	20
CGATTTA	20	7.0269307E-4	45.0	10
CGCTGTT	20	7.0269307E-4	45.0	35
TTATCGT	20	7.0269307E-4	45.0	15
CTCGCAC	20	7.0269307E-4	45.0	40
ACGTATG	20	7.0269307E-4	45.0	1
CGCACGA	20	7.0269307E-4	45.0	39
AGGGCGT	20	7.0269307E-4	45.0	6
TATCGTA	20	7.0269307E-4	45.0	16
CGTCAGT	20	7.0269307E-4	45.0	11
CGTTTTA	275	0.0	41.727272	1
TCGTGAC	50	1.0768417E-9	40.5	18
TACGGGA	95	0.0	40.263157	4
GTCATAC	45	1.9221261E-8	40.000004	39
CGTATGG	45	1.9221261E-8	40.000004	2
ACGGGCC	45	1.9221261E-8	40.000004	5
CGGTAGT	90	0.0	40.000004	12
CGAAGGA	85	0.0	39.705883	4
>>END_MODULE