##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934650.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	371948
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.203689763085162	31.0	31.0	33.0	28.0	34.0
2	31.276183767623433	31.0	31.0	34.0	28.0	34.0
3	31.107423618355256	31.0	31.0	34.0	28.0	34.0
4	35.08696645767688	35.0	35.0	37.0	32.0	37.0
5	35.26774979298182	35.0	35.0	37.0	33.0	37.0
6	35.21832621764332	37.0	35.0	37.0	32.0	37.0
7	30.85925989654468	35.0	35.0	37.0	0.0	37.0
8	32.949576284857024	35.0	35.0	37.0	17.0	37.0
9	36.4388086506716	39.0	35.0	39.0	32.0	39.0
10	36.179538537645044	37.0	35.0	39.0	32.0	39.0
11	36.376692440878834	38.0	35.0	39.0	32.0	39.0
12	36.3810693967974	38.0	35.0	39.0	32.0	39.0
13	36.31765730693538	38.0	35.0	39.0	32.0	39.0
14	37.43457956488541	39.0	36.0	41.0	32.0	41.0
15	37.643587275640684	39.0	36.0	41.0	33.0	41.0
16	37.565291384817236	39.0	36.0	41.0	33.0	41.0
17	37.50655736823427	39.0	36.0	41.0	33.0	41.0
18	37.42359953541893	39.0	36.0	40.0	32.0	41.0
19	37.249671997160895	39.0	36.0	40.0	32.0	41.0
20	37.245313861077356	39.0	35.0	40.0	32.0	41.0
21	37.22790820222182	39.0	35.0	40.0	32.0	41.0
22	37.298439566821166	39.0	35.0	40.0	32.0	41.0
23	37.345473023110756	39.0	35.0	41.0	32.0	41.0
24	37.29104068310624	39.0	35.0	41.0	32.0	41.0
25	36.923032251820146	39.0	35.0	40.0	31.0	41.0
26	36.894547087227245	39.0	35.0	40.0	31.0	41.0
27	36.889110843451235	39.0	35.0	40.0	31.0	41.0
28	36.89848580984439	39.0	35.0	40.0	31.0	41.0
29	36.894055082968585	39.0	35.0	40.0	31.0	41.0
30	36.690876143977114	39.0	35.0	40.0	31.0	41.0
31	36.686117414262206	39.0	35.0	40.0	31.0	41.0
32	36.55109585210836	39.0	35.0	40.0	31.0	41.0
33	36.435383440696015	39.0	35.0	40.0	30.0	41.0
34	36.390694398141676	39.0	35.0	40.0	30.0	41.0
35	36.33617602460559	39.0	35.0	40.0	30.0	41.0
36	36.2664861754869	38.0	35.0	40.0	30.0	41.0
37	36.18898878337832	38.0	35.0	40.0	30.0	41.0
38	36.07758880273587	38.0	35.0	40.0	30.0	41.0
39	36.030023013969696	38.0	35.0	40.0	30.0	41.0
40	35.93809080839257	38.0	35.0	40.0	29.0	41.0
41	35.76120049039113	38.0	35.0	40.0	29.0	41.0
42	35.74943809349694	38.0	35.0	40.0	29.0	41.0
43	35.676282706184736	38.0	35.0	40.0	29.0	41.0
44	35.50036833105703	38.0	34.0	40.0	28.0	41.0
45	35.434531171023906	38.0	34.0	40.0	28.0	41.0
46	35.34092400012905	38.0	34.0	40.0	28.0	41.0
47	35.278321163173345	37.0	34.0	40.0	28.0	41.0
48	35.190158301698084	37.0	34.0	40.0	27.0	41.0
49	35.05609386258294	37.0	34.0	40.0	27.0	41.0
50	34.90669125791777	37.0	34.0	40.0	27.0	41.0
51	33.30326819878047	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	17.0
9	34.0
10	38.0
11	35.0
12	21.0
13	23.0
14	27.0
15	42.0
16	61.0
17	95.0
18	206.0
19	335.0
20	561.0
21	818.0
22	1184.0
23	1637.0
24	2282.0
25	2987.0
26	3962.0
27	4656.0
28	5442.0
29	6407.0
30	8270.0
31	10340.0
32	13810.0
33	19595.0
34	31545.0
35	33507.0
36	38160.0
37	55426.0
38	71926.0
39	58494.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.858410315420436	20.99271941239098	24.691085850710316	11.457784421478271
2	32.32333552001892	23.751976082678226	26.68652607353716	17.23816232376569
3	34.13891189090948	22.991654747437813	26.968017034639253	15.901416327013454
4	30.806725671330398	26.16629206233129	24.404755503457473	18.622226762880835
5	25.911955434630645	30.922602084162303	23.152698764343402	20.01274371686365
6	27.45625732629292	36.530106358953404	22.32543258735092	13.688203727402756
7	72.59348080914536	17.78178670136686	6.4226719864067014	3.202060503081076
8	85.21325561637649	5.431942099433254	6.204630754836698	3.1501715293535653
9	79.32668007355866	7.773398432038888	7.806198715949542	5.093722778452902
10	45.71553012786734	26.645929000828072	14.360071837998861	13.278469033305731
11	35.03150978093712	25.64552034155312	21.251088861883918	18.07188101562584
12	32.37576220331874	23.099734371471282	25.447912073730738	19.076591351479237
13	26.121931022616064	24.63973458655511	27.04114553647284	22.197188854355986
14	21.488487638056935	28.691107359093206	26.65480120877112	23.16560379407874
15	20.928194263714282	25.746878595932763	32.028132964823044	21.29679417552991
16	23.993676535429685	23.85924914235323	30.663694925097058	21.48337939712003
17	24.517943368427844	24.4211556454128	27.16992697904008	23.890974007119276
18	25.104046802241175	25.14034219837182	26.925537978427094	22.83007302095992
19	27.486906771914356	26.574951337283707	24.25634766150107	21.68179422930087
20	29.361900050544698	24.13697613644918	25.248690677191437	21.252433135814684
21	27.8108767892286	25.714884876380566	24.532461526880102	21.941776807510728
22	25.94959510469205	24.732489487777862	24.956714379429382	24.3612010281007
23	25.912761998989104	26.2872767161001	25.45302031466764	22.34694097024315
24	24.981986729327755	24.30339724907783	27.786411003688688	22.92820501790573
25	24.986019551120048	25.763816447460396	26.10311118758536	23.147052813834193
26	24.767709464763893	28.22464430511792	24.81852301934679	22.189123210771395
27	24.646455956208932	27.788024132405603	25.305956746642007	22.259563164743458
28	22.46308623786121	27.464322969877507	27.573478013055585	22.499112779205696
29	23.459462075343865	26.920160882704035	27.030122490240572	22.59025455171153
30	24.40986374439438	26.46337660103025	25.79796100530182	23.328798649273555
31	26.05875014787013	27.336079236882576	23.211309107724738	23.393861507522555
32	26.71798208351705	27.915192446255926	23.653844085732416	21.712981384494608
33	26.836815898996637	27.156484239732436	23.98776173013432	22.01893813113661
34	24.12649079978922	27.713551356641254	25.082538419348943	23.07741942422059
35	24.884930151526557	26.6343682450235	24.71393850753331	23.766763095916634
36	25.619172572510134	28.766924408788324	23.126888704872727	22.487014313828816
37	24.934668286964843	29.857937130996802	24.62333444459978	20.584060137438566
38	24.12460881628615	28.453977437706346	23.995827373718907	23.4255863722886
39	24.794057233806875	27.90820222181595	24.639465731768954	22.658274812608216
40	25.679933754180688	26.315506468646156	26.151236194306733	21.85332358286642
41	22.126211190811617	27.227999612849107	26.416327013453493	24.22946218288578
42	23.729392280641378	26.98898770795918	24.918805854581823	24.36281415681762
43	24.02190628797574	26.137255745426778	25.49630593523826	24.344532031359222
44	23.225289556604686	26.661791433211096	26.138868874143697	23.97405013604052
45	22.963693849677913	27.98025530450493	24.351253401013047	24.704797444804115
46	22.972297202834806	28.832793831395787	25.24170045275146	22.953208513017948
47	24.010614386957315	26.637594502457336	26.23995827373719	23.111832836848162
48	23.631797993267874	25.54658178024885	26.991676255820707	23.829943970662566
49	22.57815608633465	26.71717551915859	27.061040790648157	23.643627603858604
50	21.24382978265779	27.65520986804607	26.83654704421048	24.264413305085657
51	22.423833439082884	27.537989181283407	25.243044726682225	24.795132652951487
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	151.0
1	374.0
2	597.0
3	1186.0
4	1775.0
5	1366.5
6	958.0
7	894.5
8	831.0
9	826.0
10	821.0
11	834.5
12	848.0
13	840.0
14	832.0
15	804.5
16	777.0
17	768.0
18	759.0
19	830.5
20	902.0
21	922.0
22	942.0
23	1053.0
24	1164.0
25	1648.5
26	2530.5
27	2928.0
28	3471.0
29	4014.0
30	5040.0
31	6066.0
32	6738.5
33	7411.0
34	8518.5
35	9626.0
36	9771.0
37	9916.0
38	10826.0
39	11736.0
40	13748.5
41	15761.0
42	18066.5
43	20372.0
44	22154.5
45	23937.0
46	25824.0
47	27711.0
48	27499.5
49	27288.0
50	26305.0
51	25322.0
52	23322.5
53	21323.0
54	21084.5
55	20846.0
56	20763.0
57	20680.0
58	21021.5
59	21363.0
60	21204.0
61	21045.0
62	19347.5
63	17650.0
64	15213.0
65	12776.0
66	11046.0
67	9316.0
68	8294.5
69	7273.0
70	6348.0
71	5423.0
72	4466.5
73	3510.0
74	2891.5
75	1803.0
76	1333.0
77	1046.5
78	760.0
79	602.0
80	444.0
81	311.0
82	178.0
83	134.0
84	90.0
85	60.5
86	31.0
87	30.5
88	30.0
89	20.0
90	10.0
91	10.0
92	10.0
93	6.5
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	371948.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.38826174931136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.39641244108891	19.006471083997102
2	8.232310661225748	4.509373583825946
3	3.9719121352039477	3.263513076126957
4	2.545629389822578	2.7888145618080213
5	1.8829059719683578	2.578476080480545
6	1.3412159935772467	2.20401448166738
7	1.1023552993392605	2.11341168353309
8	0.8295148472740219	1.8175175809664756
9	0.7465633610713025	1.8402365470919835
>10	9.812261148196425	56.10983329591348
>50	0.10793687146100207	1.8561124533114097
>100	0.02898304881823204	1.5626819047271547
>500	0.00199883095298152	0.34954366655046015
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	673	0.1809392710809038	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	604	0.16238829083635348	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGC	495	0.13308311914568702	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTC	409	0.10996160753653737	Illumina PCR Primer Index 9 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCG	396	0.10646649531654963	No Hit
GCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGC	373	0.10028283523503284	Illumina PCR Primer Index 9 (95% over 24bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.688547861529031E-4	0.0	0.0	0.06291201995977933	0.0
2	2.688547861529031E-4	0.0	0.0	0.28901889511437084	0.0
3	2.688547861529031E-4	0.0	0.0	0.3761278458279114	0.0
4	2.688547861529031E-4	0.0	0.0	0.5186208824889501	0.0
5	2.688547861529031E-4	0.0	0.0	0.9245916095798338	0.0
6	2.688547861529031E-4	0.0	0.0	1.1644100788282232	0.0
7	2.688547861529031E-4	0.0	0.0	1.3587920892167722	0.0
8	2.688547861529031E-4	0.0	0.0	1.7179820835170507	0.0
9	2.688547861529031E-4	0.0	0.0	1.8330519319904932	0.0
10	2.688547861529031E-4	0.0	0.0	2.2411734973706	0.0
11	2.688547861529031E-4	0.0	0.0	2.6783313796552206	0.0
12	2.688547861529031E-4	0.0	0.0	3.162538849516599	0.0
13	2.688547861529031E-4	0.0	0.0	3.3590716981943713	0.0
14	2.688547861529031E-4	0.0	0.0	3.4504823254863584	0.0
15	2.688547861529031E-4	0.0	0.0	3.592437652575091	0.0
16	2.688547861529031E-4	0.0	0.0	3.854033359501866	0.0
17	2.688547861529031E-4	0.0	0.0	4.1395571423962485	0.0
18	2.688547861529031E-4	0.0	0.0	4.473474786798155	0.0
19	2.688547861529031E-4	0.0	0.0	4.658984589243658	0.0
20	2.688547861529031E-4	0.0	0.0	4.859012550141418	0.0
21	2.688547861529031E-4	0.0	0.0	5.112811468269758	0.0
22	2.688547861529031E-4	0.0	0.0	5.369836643831934	0.0
23	2.688547861529031E-4	0.0	0.0	5.618527321023369	0.0
24	2.688547861529031E-4	0.0	0.0	5.823932377644187	0.0
25	2.688547861529031E-4	0.0	0.0	6.010517599234301	0.0
26	2.688547861529031E-4	0.0	0.0	6.184197791089077	0.0
27	2.688547861529031E-4	0.0	0.0	6.363523933453063	0.0
28	2.688547861529031E-4	0.0	0.0	6.538010689666297	0.0
29	2.688547861529031E-4	0.0	0.0	6.73642552184714	0.0
30	2.688547861529031E-4	0.0	0.0	6.95124049598331	0.0
31	2.688547861529031E-4	0.0	0.0	7.158527536107198	0.0
32	2.688547861529031E-4	0.0	0.0	7.367158850161851	0.0
33	2.688547861529031E-4	0.0	0.0	7.5615408605504	0.0
34	2.688547861529031E-4	0.0	0.0	7.776086979900416	0.0
35	2.688547861529031E-4	0.0	0.0	8.011603772570359	0.0
36	2.688547861529031E-4	0.0	0.0	8.240668050372634	0.0
37	2.688547861529031E-4	0.0	0.0	8.461935539376473	0.0
38	2.688547861529031E-4	0.0	0.0	8.68965554324798	0.0
39	2.688547861529031E-4	0.0	0.0	8.925710045490229	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGC	20	7.027126E-4	45.0	12
ACAAACG	20	7.027126E-4	45.0	33
GGCACGT	20	7.027126E-4	45.0	34
ACTTCGA	20	7.027126E-4	45.0	10
GACTTCG	20	7.027126E-4	45.0	9
GTTGTCC	25	3.8857004E-5	45.0	9
CTACGAA	80	0.0	45.0	11
GTACTTG	20	7.027126E-4	45.0	38
CTCGCTA	20	7.027126E-4	45.0	14
GCGATAT	20	7.027126E-4	45.0	9
ACGTCCA	20	7.027126E-4	45.0	37
ACCACGC	20	7.027126E-4	45.0	25
TATAGCG	20	7.027126E-4	45.0	1
CGATCAC	25	3.8857004E-5	45.0	12
ACGACGA	25	3.8857004E-5	45.0	33
TGCAACG	25	3.8857004E-5	45.0	1
ACACGAC	30	2.161527E-6	44.999996	26
ACCTATG	65	0.0	44.999996	34
CGTTTAA	30	2.161527E-6	44.999996	15
TACGAAT	85	0.0	42.35294	12
>>END_MODULE