##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934649.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	588833
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.159231904461876	31.0	31.0	33.0	28.0	34.0
2	31.23167519483453	31.0	31.0	34.0	28.0	34.0
3	31.002844609592195	31.0	31.0	34.0	28.0	34.0
4	34.96718933891273	35.0	35.0	37.0	32.0	37.0
5	35.15818916399047	35.0	35.0	37.0	33.0	37.0
6	35.107229044567816	36.0	35.0	37.0	32.0	37.0
7	30.685511851407785	35.0	35.0	37.0	0.0	37.0
8	32.861656870453935	35.0	35.0	37.0	17.0	37.0
9	36.32929879948984	39.0	35.0	39.0	32.0	39.0
10	36.046062296100935	37.0	35.0	39.0	32.0	39.0
11	36.23246149587404	37.0	35.0	39.0	32.0	39.0
12	36.19025428262343	37.0	35.0	39.0	32.0	39.0
13	36.05954149988197	38.0	35.0	39.0	31.0	39.0
14	36.93842057085795	39.0	36.0	40.0	31.0	41.0
15	37.18858318063016	39.0	36.0	41.0	32.0	41.0
16	37.16943174040857	39.0	35.0	40.0	32.0	41.0
17	37.113156022165875	39.0	35.0	40.0	32.0	41.0
18	37.04706088143837	39.0	35.0	40.0	32.0	41.0
19	36.864652626466246	39.0	35.0	40.0	31.0	41.0
20	36.834044627254244	39.0	35.0	40.0	31.0	41.0
21	36.82198178430897	39.0	35.0	40.0	31.0	41.0
22	36.958733630757784	39.0	35.0	40.0	31.0	41.0
23	37.02441609081013	39.0	35.0	40.0	32.0	41.0
24	36.96738464046682	39.0	35.0	40.0	32.0	41.0
25	36.60979089147517	38.0	35.0	40.0	31.0	41.0
26	36.59040848593744	38.0	35.0	40.0	31.0	41.0
27	36.59111496808093	38.0	35.0	40.0	31.0	41.0
28	36.551467054326096	38.0	35.0	40.0	31.0	41.0
29	36.49798160089533	38.0	35.0	40.0	31.0	41.0
30	36.28440831271345	38.0	35.0	40.0	30.0	41.0
31	36.252487547403085	38.0	35.0	40.0	30.0	41.0
32	36.10246029009923	38.0	35.0	40.0	30.0	41.0
33	35.95275061010507	38.0	35.0	40.0	29.0	41.0
34	35.85569253081943	38.0	35.0	40.0	29.0	41.0
35	35.775571341959434	38.0	35.0	40.0	29.0	41.0
36	35.68615210085033	38.0	34.0	40.0	29.0	41.0
37	35.597471609097994	38.0	34.0	40.0	28.0	41.0
38	35.42760001562412	38.0	34.0	40.0	27.0	41.0
39	35.324400976168114	38.0	34.0	40.0	27.0	41.0
40	35.19205445346983	38.0	34.0	40.0	26.0	41.0
41	34.98697763202809	38.0	34.0	40.0	25.0	41.0
42	35.016469864970205	38.0	34.0	40.0	26.0	41.0
43	34.99221171367773	37.0	34.0	40.0	26.0	41.0
44	34.82558042772739	37.0	34.0	40.0	25.0	41.0
45	34.73166415605104	37.0	34.0	40.0	25.0	41.0
46	34.66996414942777	37.0	34.0	40.0	25.0	41.0
47	34.58551745571325	37.0	33.0	40.0	25.0	41.0
48	34.496485421163555	37.0	33.0	40.0	24.0	41.0
49	34.37561074192513	36.0	33.0	40.0	24.0	41.0
50	34.223433469251894	36.0	33.0	40.0	24.0	41.0
51	32.528433358864056	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	6.0
8	25.0
9	56.0
10	61.0
11	46.0
12	36.0
13	29.0
14	44.0
15	71.0
16	135.0
17	261.0
18	412.0
19	719.0
20	1282.0
21	1908.0
22	2665.0
23	3648.0
24	4770.0
25	6397.0
26	7822.0
27	9110.0
28	10339.0
29	12328.0
30	15112.0
31	18890.0
32	24904.0
33	34132.0
34	51141.0
35	52445.0
36	60322.0
37	85889.0
38	104778.0
39	79047.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.46155701192019	21.0366946146021	25.75942584739646	12.742322526081248
2	33.790565406490465	23.369953789954028	25.53491397391111	17.3045668296444
3	32.424813147360965	23.614505301163486	27.147934983263504	16.812746568212038
4	29.156993578824558	25.06381265995622	26.888948139795154	18.89024562142407
5	26.38405116561062	29.71708447046956	24.877172305220665	19.021692058699156
6	26.47490884512247	34.64004225306666	24.807882710377985	14.077166191432886
7	71.07957604278293	17.633522577708792	8.153585142137075	3.1333162373712073
8	84.26990335120485	4.958112062333463	7.506882256938724	3.2651023295229717
9	78.7496624679663	6.37803927429339	9.111072239497446	5.761226018242863
10	46.79102563884837	23.904400738409702	15.698508745263936	13.606064877477994
11	35.32206924543971	24.401145995553918	22.606069972301146	17.67071478670523
12	30.976015270883256	22.082661807337566	27.544482051787178	19.396840869992
13	27.38008909147415	23.131176411648124	29.913065334313803	19.57566916256392
14	22.28781335285217	25.32313915830125	31.1787892322611	21.210258256585483
15	21.52715625652774	23.71877934830419	34.21360555539516	20.540458839772906
16	26.883004179453255	23.152065186563934	28.948275657104816	21.016654976877994
17	27.01988509475522	23.097720406295164	26.65152938099597	23.23086511795365
18	28.01388509135867	22.292228866249005	28.394808035555073	21.299078006837252
19	27.35325635621645	25.148386724249487	25.611166493725722	21.88719042580834
20	27.629905253272153	26.155123778728434	26.45690713665844	19.758063831340973
21	26.86941798438606	25.301231418755403	28.48702433457364	19.342326262284892
22	26.068172130298407	22.460697685082188	28.85470073857953	22.61642944603988
23	24.615977705053897	24.060302326805733	29.05221004936884	22.271509918771535
24	24.904854177670067	24.070831627982805	28.74414307622025	22.28017111812687
25	24.854245601044777	25.80714735756997	26.238848705830005	23.099758335555244
26	23.25735819833467	25.412977873183056	26.49834503161338	24.831318896868893
27	23.63471476632594	24.340177945189893	28.256908155623073	23.7681991328611
28	21.73995003676764	25.10932641343131	28.41943301411436	24.73129053568669
29	23.99305066122313	24.497438153092645	28.002336825551556	23.50717436013267
30	25.386145137925354	24.37872877369305	27.916234314313225	22.31889177406837
31	26.518724324214165	23.878417140343696	26.886400728220057	22.71645780722208
32	27.64620868735278	24.070322145667784	26.16870997379563	22.114759193183804
33	26.794354256639828	23.826789599088364	27.018356647810158	22.360499496461646
34	23.567972583058356	24.217053052393464	29.533501009624118	22.681473354924062
35	23.22373236554337	26.715044842935093	27.449039031440154	22.61218376008138
36	25.857416279318585	26.72098880327699	25.09998590432262	22.321609013081808
37	24.095456606542093	27.099873818213315	26.727781900810587	22.076887674434005
38	23.937007606570962	26.899647268410572	26.187560819451356	22.975784305567114
39	24.37923825600807	24.926422262339237	27.190391842848484	23.503947638804213
40	25.8647188591672	23.71504314466071	27.406921826731857	23.01331616944023
41	22.12291091022412	25.124950537758583	27.23284870243346	25.51928984958384
42	22.8633585413861	24.002900652646847	27.780372363641305	25.353368442325753
43	22.68554921344422	23.806749961364257	28.5289717118436	24.978729113347928
44	23.416146853182482	23.968255855225507	27.888382614425485	24.727214677166533
45	22.214278072050988	24.442583890508853	27.182749608123185	26.16038842931697
46	23.509382116831087	25.110515205499688	27.0546997196149	24.32540295805432
47	21.804484463336806	25.528120876377514	29.405111466239152	23.262283194046528
48	22.358971049516583	25.438112334057365	28.06873935394246	24.13417726248359
49	23.20929703328448	24.350367591490286	28.914819651751856	23.52551572347338
50	21.628373409778323	25.275927130442753	28.42248990800448	24.67320955177444
51	22.133440211401194	24.874285238768888	27.38365546767929	25.608619082150625
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	183.0
1	502.5
2	822.0
3	2469.0
4	4116.0
5	2907.0
6	1698.0
7	1561.5
8	1425.0
9	1396.0
10	1367.0
11	1373.5
12	1380.0
13	1354.5
14	1329.0
15	1458.0
16	1587.0
17	1531.0
18	1475.0
19	1478.0
20	1481.0
21	1625.0
22	1769.0
23	2033.0
24	2297.0
25	2744.5
26	4004.0
27	4816.0
28	5181.0
29	5546.0
30	6623.5
31	7701.0
32	9169.5
33	10638.0
34	12673.5
35	14709.0
36	15820.0
37	16931.0
38	18246.5
39	19562.0
40	21853.5
41	24145.0
42	26768.0
43	29391.0
44	30982.5
45	32574.0
46	39092.0
47	45610.0
48	43631.0
49	41652.0
50	42485.0
51	43318.0
52	40992.0
53	38666.0
54	37906.0
55	37146.0
56	36688.0
57	36230.0
58	35186.5
59	34143.0
60	31911.5
61	29680.0
62	27581.5
63	25483.0
64	22909.0
65	20335.0
66	17691.5
67	15048.0
68	13021.0
69	10994.0
70	9336.0
71	7678.0
72	6475.0
73	5272.0
74	4292.5
75	2614.5
76	1916.0
77	1533.5
78	1151.0
79	820.0
80	489.0
81	366.5
82	244.0
83	195.5
84	147.0
85	114.5
86	82.0
87	63.5
88	45.0
89	41.0
90	37.0
91	25.5
92	14.0
93	8.0
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	588833.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.005903195559014
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.41012895714654	23.175515777803763
2	8.384892870391793	5.367321390297854
3	3.922933888461673	3.766711268300466
4	2.371954285663228	3.0366615700491444
5	1.6632634633198438	2.6617124697862575
6	1.1989736307751335	2.302454037637011
7	0.9358742655121874	2.0967450801638576
8	0.7605664345332203	1.9474092541969377
9	0.6112428980500291	1.7607042921565959
>10	7.652836607833792	49.78732694499566
>50	0.06681477521506536	1.325344531352256
>100	0.016309118359572883	0.9515337246433399
>500	0.0015783017767328595	0.3429966726417419
>1k	0.0026305029612214326	1.4775629859751032
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTC	2350	0.3990944800987716	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1982	0.33659798278968744	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCG	1825	0.30993507497032263	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGC	1559	0.2647609763719085	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTT	1059	0.1798472572019571	No Hit
GCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	783	0.13297488422014392	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	682	0.11582231294781373	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05026892174861124	0.0
2	0.0	0.0	0.0	0.3077273182719039	0.0
3	0.0	0.0	0.0	0.4254177330414566	0.0
4	0.0	0.0	0.0	0.6349847919528967	0.0
5	0.0	0.0	0.0	1.3953022334006415	0.0
6	0.0	0.0	0.0	1.8692906138073104	0.0
7	0.0	0.0	0.0	2.160035188245224	0.0
8	0.0	0.0	0.0	2.6031149748740305	0.0
9	0.0	0.0	0.0	2.790774294239623	0.0
10	0.0	0.0	0.0	3.5065969468423135	0.0
11	0.0	0.0	0.0	4.03611889958613	0.0
12	0.0	0.0	0.0	4.724769162054437	0.0
13	0.0	0.0	0.0	4.947582761156389	0.0
14	0.0	0.0	0.0	5.076481786856375	0.0
15	0.0	0.0	0.0	5.2286471716089284	0.0
16	0.0	0.0	0.0	5.476595231585186	0.0
17	0.0	0.0	0.0	5.756301022531006	0.0
18	0.0	0.0	0.0	6.097314518717531	0.0
19	0.0	0.0	0.0	6.308070369697351	0.0
20	0.0	0.0	0.0	6.513391742650293	0.0
21	0.0	0.0	0.0	6.765925143461729	0.0
22	0.0	0.0	0.0	7.041724903325731	0.0
23	0.0	0.0	0.0	7.28848417123361	0.0
24	0.0	0.0	0.0	7.501787433788527	0.0
25	0.0	0.0	0.0	7.698787262262814	0.0
26	0.0	0.0	0.0	7.886616409066748	0.0
27	0.0	0.0	0.0	8.136942053179764	0.0
28	0.0	0.0	0.0	8.332922917024012	0.0
29	0.0	0.0	0.0	8.579172702616871	0.0
30	0.0	0.0	0.0	8.900486215955967	0.0
31	0.0	0.0	0.0	9.137904974755152	0.0
32	0.0	0.0	0.0	9.358680644597024	0.0
33	0.0	0.0	0.0	9.592872682067751	0.0
34	0.0	0.0	0.0	9.84048108716733	0.0
35	0.0	0.0	0.0	10.113054125702874	0.0
36	0.0	0.0	0.0	10.357605636912334	0.0
37	0.0	0.0	0.0	10.619309719394124	0.0
38	0.0	0.0	0.0	10.971701653949422	0.0
39	0.0	0.0	0.0	11.56983389178256	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAT	20	7.0304796E-4	45.000004	22
CTACGCG	20	7.0304796E-4	45.000004	1
GTCTACG	25	3.88848E-5	45.0	1
CTACGCC	25	3.88848E-5	45.0	24
CGCTACA	25	3.88848E-5	45.0	30
ACTACGC	25	3.88848E-5	45.0	23
ACGGTTC	30	2.1636915E-6	44.999996	11
TATGCGG	70	0.0	41.785713	2
CGGGTAT	60	3.6379788E-12	41.249996	6
TACGAAT	100	0.0	40.5	12
ATATGCG	40	3.4543882E-7	39.375004	1
TTGTACG	35	6.243099E-6	38.571426	1
TACGGGT	35	6.243099E-6	38.571426	4
CGTTTTT	1275	0.0	37.588238	1
GACCGAT	240	0.0	37.499996	9
GTTACGG	60	1.546141E-10	37.499996	2
TACGCCG	30	1.139246E-4	37.499996	25
CATCGAA	30	1.139246E-4	37.499996	15
ACGTATG	30	1.139246E-4	37.499996	1
TACGCGG	55	2.744855E-9	36.81818	2
>>END_MODULE