##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934647.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	462877
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95413252332693	31.0	30.0	33.0	28.0	34.0
2	31.023276161917746	31.0	31.0	34.0	28.0	34.0
3	30.65474197248945	31.0	30.0	34.0	27.0	34.0
4	34.62824681286821	35.0	35.0	37.0	32.0	37.0
5	34.982626054005706	35.0	35.0	37.0	32.0	37.0
6	35.01019277259402	35.0	35.0	37.0	32.0	37.0
7	31.09033501340529	35.0	35.0	37.0	0.0	37.0
8	33.128431527165965	35.0	35.0	37.0	17.0	37.0
9	36.38281876178769	39.0	35.0	39.0	32.0	39.0
10	35.779667168599865	37.0	35.0	39.0	31.0	39.0
11	36.17311294361137	37.0	35.0	39.0	32.0	39.0
12	36.35427770228376	38.0	35.0	39.0	32.0	39.0
13	36.34537036837	38.0	35.0	39.0	32.0	39.0
14	37.40158184571711	39.0	36.0	40.0	32.0	41.0
15	37.59060830414992	39.0	36.0	41.0	32.0	41.0
16	37.521691507679144	39.0	36.0	40.0	32.0	41.0
17	37.43147963713903	39.0	36.0	40.0	32.0	41.0
18	37.28214406851064	39.0	36.0	40.0	32.0	41.0
19	36.96889670474014	38.0	36.0	40.0	32.0	41.0
20	36.87155767082832	38.0	35.0	40.0	32.0	41.0
21	36.8770969393597	38.0	35.0	40.0	32.0	41.0
22	36.95848141082836	39.0	35.0	40.0	32.0	41.0
23	37.030407646091724	39.0	35.0	40.0	32.0	41.0
24	36.96020325053956	39.0	35.0	40.0	32.0	41.0
25	36.65443303512596	38.0	35.0	40.0	31.0	41.0
26	36.67592470569935	38.0	35.0	40.0	31.0	41.0
27	36.738988975472964	38.0	35.0	40.0	31.0	41.0
28	36.71924506942449	38.0	35.0	40.0	31.0	41.0
29	36.664980113507475	38.0	35.0	40.0	31.0	41.0
30	36.41762714500828	38.0	35.0	40.0	30.0	41.0
31	36.32879577079872	38.0	35.0	40.0	30.0	41.0
32	36.053461286691714	38.0	35.0	40.0	30.0	41.0
33	35.74118394303454	38.0	35.0	40.0	28.0	41.0
34	35.526813818789876	38.0	35.0	40.0	26.0	41.0
35	35.33846572631606	38.0	35.0	40.0	25.0	41.0
36	35.20485787801079	38.0	35.0	40.0	24.0	41.0
37	35.13108449976992	38.0	35.0	40.0	24.0	41.0
38	34.998574135245434	38.0	34.0	40.0	23.0	41.0
39	34.99816149862706	38.0	34.0	40.0	23.0	41.0
40	34.92151694726677	38.0	34.0	40.0	23.0	41.0
41	34.79249563058869	38.0	34.0	40.0	23.0	41.0
42	34.80250260868438	38.0	34.0	40.0	23.0	41.0
43	34.75777150301268	38.0	34.0	40.0	23.0	41.0
44	34.59391587830028	38.0	34.0	40.0	22.0	41.0
45	34.51783519163838	38.0	34.0	40.0	22.0	41.0
46	34.39628238171264	38.0	34.0	40.0	21.0	41.0
47	34.36028577786323	38.0	33.0	40.0	21.0	41.0
48	34.27027914543172	38.0	33.0	40.0	20.0	41.0
49	34.149975047366794	38.0	33.0	40.0	20.0	41.0
50	33.991062420470236	38.0	33.0	40.0	20.0	41.0
51	32.29753692665654	36.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	17.0
10	29.0
11	16.0
12	11.0
13	10.0
14	13.0
15	18.0
16	60.0
17	112.0
18	260.0
19	562.0
20	927.0
21	1429.0
22	2259.0
23	3258.0
24	5088.0
25	7502.0
26	9244.0
27	9394.0
28	9199.0
29	9200.0
30	10795.0
31	13213.0
32	17162.0
33	23065.0
34	33938.0
35	39158.0
36	46668.0
37	67984.0
38	88893.0
39	63388.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.47184241169901	21.775115203390964	26.913197242463983	15.839845142446048
2	37.014800908232644	23.432142880290012	24.144643177345166	15.40841303413218
3	28.26431211747397	22.418914744089683	33.11182020277525	16.204952935661094
4	25.629270843010133	23.90289428941166	32.662240724857796	17.80559414272042
5	23.85839002586	27.429749155823252	31.61768677207984	17.094174046236905
6	22.712945339690673	33.12845529157854	30.762383959453594	13.396215409277195
7	66.18172862337079	16.43136297547729	14.037206428489643	3.349701972662284
8	78.38972340384163	4.419964699045319	13.351279065496882	3.8390328316161746
9	73.73146645869205	5.3781026060918995	14.979789447304576	5.910641487911476
10	36.22927905253447	22.547890692343756	26.004964601827268	15.217865653294504
11	26.838015282677684	26.24131248690257	29.191988368400246	17.7286838620195
12	22.847538330917285	22.88253682943849	35.630631895730396	18.639292943913826
13	20.708309118837185	26.419977661452176	36.953661555877694	15.91805166383294
14	18.88881927596316	28.89817381291358	34.86390552997881	17.349101381144454
15	17.876671340334475	27.148032846738985	38.86885716939921	16.10643864352733
16	20.146388781468943	25.46291995497724	34.98337571320243	19.407315550351388
17	20.354651451681548	25.70358864233911	32.681684335147345	21.260075570831994
18	21.918133759076387	25.016581078774706	34.30544183443982	18.75984332770909
19	20.614763749332976	28.047623882802558	32.43561464492727	18.901997722937196
20	21.008388837639373	26.790702497639764	34.51780926682466	17.6830993978962
21	20.10987800214744	27.47706194086118	34.88140477923941	17.531655277751973
22	18.80283531046045	24.918930947098257	34.25445636745831	22.023777374982988
23	17.771891884885186	26.96439874955982	33.726886408268285	21.53682295728671
24	19.263216794094326	25.795189650814365	34.36809346759506	20.573500087496246
25	17.5554196903281	28.405170272016107	32.922785102737876	21.116624934917915
26	17.249290848324716	29.424663571531962	33.54174003028882	19.784305549854498
27	18.801971150003133	28.42547804276298	32.732885842243185	20.0396649649907
28	17.680938996752918	26.63839421703822	35.169602291753534	20.51106449445533
29	18.152770606446207	26.49429546078116	34.08961776022572	21.26331617254692
30	18.639725024142486	27.788591785722772	32.45203369361623	21.119649496518512
31	19.9119852574226	27.7257241124532	30.225956355576105	22.136334274548098
32	18.888387195734506	29.1103252051841	30.023526768450363	21.977760830631034
33	19.168807264132802	28.129071005904375	30.12765810355667	22.574463626406153
34	18.49411398708512	28.742192850368458	29.87532325002106	22.88836991252536
35	16.9109720292864	30.16848968516474	29.27646005310266	23.6440782324462
36	19.193867917394904	28.93576479280673	30.24021500312178	21.63015228667659
37	18.44766536250451	28.56460787639049	31.05317395333966	21.934552807765346
38	18.80067490931716	29.664252058322187	28.55726251250332	22.97781051985733
39	19.514903527286947	27.12102783244793	30.453014515735283	22.91105412452984
40	19.13488896618324	28.703089589675013	29.831251066698066	22.330770377443685
41	17.765410681455332	28.09860934978407	29.978590424670053	24.157389544090545
42	19.353305521769283	27.612735132659434	30.902810033767068	22.131149311804215
43	19.956273480859927	26.56688493919551	30.705349369270884	22.771492210673678
44	19.332133590565096	27.30271756859814	28.817158770040418	24.547990070796345
45	19.130784204011	27.755753688344853	27.909790289861018	25.203671817783125
46	20.81352065451513	28.511462008265692	28.229745699181425	22.44527163803775
47	17.49600865888778	27.928801819921922	31.126843632325652	23.448345888864644
48	18.803915511032088	26.71249597625287	30.674023552693264	23.809564960021774
49	19.514255406943963	24.26994644365566	32.57366427798314	23.642133871417244
50	18.85706137915688	25.197406654467603	31.138725838613713	24.806806127761803
51	18.211965597772195	25.712446287026573	28.453563257625675	27.622024857575557
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	110.0
1	256.5
2	403.0
3	4227.0
4	8051.0
5	5999.5
6	3948.0
7	3789.5
8	3631.0
9	3610.0
10	3589.0
11	3517.5
12	3446.0
13	3380.0
14	3314.0
15	3163.5
16	3013.0
17	2918.5
18	2824.0
19	2751.5
20	2679.0
21	2656.5
22	2634.0
23	2873.0
24	3112.0
25	3374.5
26	3876.0
27	4115.0
28	5049.0
29	5983.0
30	7009.0
31	8035.0
32	9237.5
33	10440.0
34	12320.5
35	14201.0
36	15925.0
37	17649.0
38	18948.5
39	20248.0
40	23006.5
41	25765.0
42	28370.5
43	30976.0
44	34580.0
45	38184.0
46	45156.0
47	52128.0
48	50652.0
49	49176.0
50	46806.5
51	44437.0
52	38292.0
53	32147.0
54	26959.0
55	21771.0
56	18109.5
57	14448.0
58	12619.5
59	10791.0
60	9000.0
61	7209.0
62	6076.5
63	4944.0
64	3892.0
65	2840.0
66	2074.5
67	1309.0
68	1015.5
69	722.0
70	567.0
71	412.0
72	347.0
73	282.0
74	205.5
75	88.5
76	48.0
77	49.5
78	51.0
79	33.5
80	16.0
81	13.5
82	11.0
83	10.0
84	9.0
85	6.5
86	4.0
87	3.0
88	2.0
89	2.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	462877.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.278446245893754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.94423783509724	36.071382208287055
2	9.630536905251825	8.91372568977585
3	3.5264095673079967	4.8959026680500575
4	1.909525278700655	3.534794518620942
5	1.2039683692530143	2.7858892729115996
6	0.8091459828319582	2.246761132294179
7	0.6378743026579597	2.0663882139035343
8	0.5460348346063537	2.02157149933725
9	0.4231256191801212	1.7623436600238975
>10	3.261084970616458	28.80564635368696
>50	0.08404381571958529	2.5759909355013946
>100	0.01985650591165903	1.6906142600515293
>500	0.002308896036239422	0.6725272502063157
>1k	0.0018471168289915375	1.9564623373494818
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4130	0.8922456721764097	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTC	1753	0.3787183204177352	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCG	1693	0.3657559135580295	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGC	1579	0.3411273405245886	No Hit
GCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	989	0.2136636730708158	No Hit
TCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	561	0.12119850413824838	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTT	555	0.11990226345227782	No Hit
CCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	531	0.11471730070839554	No Hit
CTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGCT	511	0.11039649842182696	No Hit
GAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCT	480	0.10369925487764568	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.160401143284285E-4	0.0	0.0	0.1272476273394444	0.0
2	2.160401143284285E-4	0.0	0.0	0.7263268643721766	0.0
3	2.160401143284285E-4	0.0	0.0	1.0248943023740649	0.0
4	2.160401143284285E-4	0.0	0.0	1.5948081239724592	0.0
5	2.160401143284285E-4	0.0	0.0	3.071010225178611	0.0
6	2.160401143284285E-4	0.0	0.0	3.907517547858286	0.0
7	2.160401143284285E-4	0.0	0.0	4.610296039768664	0.0
8	2.160401143284285E-4	0.0	0.0	5.773023935084266	0.0
9	2.160401143284285E-4	0.0	0.0	6.113935235494527	0.0
10	2.160401143284285E-4	0.0	0.0	7.528565904117077	0.0
11	2.160401143284285E-4	0.0	0.0	9.150163002266261	0.0
12	2.160401143284285E-4	0.0	0.0	10.635222748159878	0.0
13	2.160401143284285E-4	0.0	0.0	11.19735912564245	0.0
14	2.160401143284285E-4	0.0	0.0	11.427225807287897	0.0
15	2.160401143284285E-4	0.0	0.0	11.81869049445101	0.0
16	2.160401143284285E-4	0.0	0.0	12.619551198266494	0.0
17	2.160401143284285E-4	0.0	0.0	13.698671569336994	0.0
18	2.160401143284285E-4	0.0	0.0	14.890996960315592	0.0
19	2.160401143284285E-4	0.0	0.0	15.591615051082686	0.0
20	2.160401143284285E-4	0.0	0.0	16.257018603214245	0.0
21	2.160401143284285E-4	0.0	0.0	17.081211639377198	0.0
22	2.160401143284285E-4	0.0	0.0	17.993549042186153	0.0
23	2.160401143284285E-4	0.0	0.0	18.910423287396004	0.0
24	2.160401143284285E-4	0.0	0.0	19.59807897130339	0.0
25	2.160401143284285E-4	0.0	0.0	20.26521084434958	0.0
26	2.160401143284285E-4	0.0	0.0	20.86169760001037	0.0
27	2.160401143284285E-4	0.0	0.0	21.482596888590272	0.0
28	2.160401143284285E-4	0.0	0.0	22.098095174311965	0.0
29	2.160401143284285E-4	0.0	0.0	22.723531305292767	0.0
30	2.160401143284285E-4	0.0	0.0	23.389150897538656	0.0
31	2.160401143284285E-4	0.0	0.0	24.09192938944903	0.0
32	2.160401143284285E-4	0.0	0.0	24.752796099179697	0.0
33	2.160401143284285E-4	0.0	0.0	25.382769072561395	0.0
34	2.160401143284285E-4	0.0	0.0	25.982064349708455	0.0
35	4.32080228656857E-4	0.0	0.0	26.604475919088657	0.0
36	4.32080228656857E-4	0.0	0.0	27.21586944263811	0.0
37	4.32080228656857E-4	0.0	0.0	27.863773745509064	0.0
38	4.32080228656857E-4	0.0	0.0	28.518375291924205	0.0
39	4.32080228656857E-4	0.0	0.0	29.183346763827107	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	35	1.209828E-7	45.0	1
GTCGATC	20	7.028916E-4	45.0	33
GCGCGAC	20	7.028916E-4	45.0	9
TTTCGCG	20	7.028916E-4	45.0	1
GGCGTCC	20	7.028916E-4	45.0	8
GCTATCG	25	3.8871833E-5	45.0	1
GCGTTAG	20	7.028916E-4	45.0	1
GCACCGG	25	3.8871833E-5	45.0	2
TACGCGG	25	3.8871833E-5	45.0	2
GAGACCG	20	7.028916E-4	45.0	10
ACGGCAA	20	7.028916E-4	45.0	5
AGACCGT	20	7.028916E-4	45.0	20
AGCGCCC	25	3.8871833E-5	45.0	13
CCGACGG	20	7.028916E-4	45.0	2
CCGACGA	25	3.8871833E-5	45.0	12
CGTAAGT	20	7.028916E-4	45.0	13
GACGACA	20	7.028916E-4	45.0	14
CGGTACT	20	7.028916E-4	45.0	31
ACCCCGC	20	7.028916E-4	45.0	12
TGTAACG	20	7.028916E-4	45.0	1
>>END_MODULE