##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934644.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	663497
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.126553699564578	31.0	31.0	33.0	28.0	34.0
2	31.16739035745452	31.0	31.0	34.0	28.0	34.0
3	30.962234946050998	31.0	31.0	34.0	28.0	34.0
4	34.95280762384758	35.0	35.0	37.0	32.0	37.0
5	35.169260750237	35.0	35.0	37.0	33.0	37.0
6	35.13738117881467	36.0	35.0	37.0	32.0	37.0
7	31.123198748449504	35.0	35.0	37.0	0.0	37.0
8	33.080416339486085	35.0	35.0	37.0	17.0	37.0
9	36.36546208950455	39.0	35.0	39.0	32.0	39.0
10	36.04612228842029	37.0	35.0	39.0	32.0	39.0
11	36.28187165880178	37.0	35.0	39.0	32.0	39.0
12	36.384800534139565	38.0	35.0	39.0	32.0	39.0
13	36.325706069507476	38.0	35.0	39.0	32.0	39.0
14	37.44209393561689	39.0	36.0	40.0	32.0	41.0
15	37.57990314952441	39.0	36.0	41.0	32.0	41.0
16	37.54655861292515	39.0	36.0	40.0	33.0	41.0
17	37.40876145634419	39.0	36.0	40.0	32.0	41.0
18	37.34425475925287	39.0	36.0	40.0	32.0	41.0
19	37.15743402004832	39.0	36.0	40.0	32.0	41.0
20	37.12660192887082	39.0	35.0	40.0	32.0	41.0
21	37.18133314845433	39.0	35.0	40.0	32.0	41.0
22	37.25396799081232	39.0	35.0	40.0	32.0	41.0
23	37.31169997754323	39.0	35.0	40.0	32.0	41.0
24	37.215122298970456	39.0	35.0	41.0	32.0	41.0
25	36.92020611999753	39.0	35.0	40.0	31.0	41.0
26	36.879747760728385	39.0	35.0	40.0	31.0	41.0
27	36.93890552632491	39.0	35.0	40.0	31.0	41.0
28	36.94453328349638	39.0	35.0	40.0	31.0	41.0
29	36.94132452746584	39.0	35.0	40.0	31.0	41.0
30	36.727955062343916	39.0	35.0	40.0	31.0	41.0
31	36.75428675638322	39.0	35.0	40.0	31.0	41.0
32	36.61164255452549	39.0	35.0	40.0	30.0	41.0
33	36.472898897809635	39.0	35.0	40.0	30.0	41.0
34	36.418821788191956	39.0	35.0	40.0	30.0	41.0
35	36.365466611002006	39.0	35.0	40.0	30.0	41.0
36	36.31987183061867	39.0	35.0	40.0	30.0	41.0
37	36.23033110925897	39.0	35.0	40.0	30.0	41.0
38	36.1786262786418	39.0	35.0	40.0	30.0	41.0
39	36.07518798125689	38.0	35.0	40.0	30.0	41.0
40	35.95346173381341	38.0	35.0	40.0	29.0	41.0
41	35.792978717311456	38.0	35.0	40.0	28.0	41.0
42	35.85185012140221	38.0	35.0	40.0	29.0	41.0
43	35.845972174704634	38.0	35.0	40.0	29.0	41.0
44	35.690278931178284	38.0	35.0	40.0	28.0	41.0
45	35.62052277553629	38.0	35.0	40.0	28.0	41.0
46	35.493197407071925	38.0	34.0	40.0	28.0	41.0
47	35.43299216123057	38.0	34.0	40.0	28.0	41.0
48	35.394924166951775	38.0	34.0	40.0	27.0	41.0
49	35.28869007697096	38.0	34.0	40.0	27.0	41.0
50	35.13797801647935	38.0	34.0	40.0	27.0	41.0
51	33.45386188633859	36.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	23.0
9	57.0
10	80.0
11	51.0
12	33.0
13	25.0
14	25.0
15	42.0
16	101.0
17	183.0
18	315.0
19	625.0
20	1022.0
21	1608.0
22	2194.0
23	3053.0
24	4317.0
25	5750.0
26	7285.0
27	8400.0
28	9803.0
29	11748.0
30	14924.0
31	18727.0
32	24281.0
33	33872.0
34	51172.0
35	56755.0
36	66931.0
37	100182.0
38	133538.0
39	106365.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.935692248796904	20.71765207679914	24.111638786610943	11.235016887793012
2	31.712426732901584	26.49281006545621	24.802222165284846	16.992541036357363
3	32.74106740497697	25.59499138654734	26.705772595806764	14.958168612668935
4	29.07805159631468	24.91510888519466	27.57691443970357	18.429925078787093
5	26.85226911350014	30.140603499337598	23.941781198709265	19.065346188453
6	25.825437040408623	37.41900867675362	23.548561636299787	13.206992646537966
7	69.82443025364094	17.63203149373697	9.182859907429876	3.360678345192216
8	80.96751002642063	7.8687620290672005	7.674337638301304	3.4893903062108795
9	75.32920269420961	8.22731677761919	9.042994919344022	7.400485608827169
10	41.29212340070867	28.38490603574696	16.377617381841965	13.945353181702405
11	32.3421206124519	26.719638521349758	23.228590332737	17.709650533461343
12	29.73502517720502	23.4050794502462	27.566213562382348	19.293681810166436
13	25.200867524645933	25.827245639392494	29.580842113830208	19.391044722131372
14	21.437474472378927	29.845801864966987	26.567716206704777	22.14900745594931
15	20.707855498969852	27.62544517910405	32.44430645504049	19.222392866885606
16	23.2781760882114	26.029507292421822	29.239469055624966	21.452847563741813
17	23.148107677954837	26.183991788960615	28.56742381653572	22.10047671654883
18	23.267023061144208	26.97495241123924	28.493723407943065	21.264301119673487
19	25.463415810470885	27.894474277954533	25.36228498395622	21.279824927618364
20	26.67864361104873	27.3021581107375	27.89854362566824	18.12065465254553
21	25.314809260629662	28.43208032590954	26.907129949344156	19.345980464116643
22	24.340275841488356	25.846085212141123	26.742698158394084	23.070940787976436
23	23.47742340960095	28.534115451916136	27.161238106577724	20.82722303190519
24	23.04441466954636	26.4337291653165	28.201031805720294	22.320824359416847
25	22.109218278304198	30.230279865621096	25.923553535283506	21.736948320791203
26	22.043355131974977	28.777974881574448	26.91330932920571	22.26536065724487
27	23.328063276849782	29.093273971095574	26.27291457233416	21.30574817972048
28	20.845459738325868	28.582194041570645	28.734116356215626	21.838229863887854
29	22.29625755655263	26.85498201197594	27.135164137893614	23.713596293577815
30	22.644714294111353	28.734267072797614	27.039609824912546	21.581408808178484
31	23.695811736903106	27.766214466681838	25.075169895267045	23.462803901148007
32	24.09249778069833	28.98460731548146	25.770124054818634	21.152770849001577
33	23.77629439168527	28.880763590490986	24.65482134809954	22.688120669724203
34	22.22391359719788	29.17797669017343	26.189116152748245	22.408993559880454
35	22.29128390934699	28.466594423185033	25.713454619990745	23.52866704747723
36	22.293092508330858	30.06825953998285	23.95986718854795	23.67878076313834
37	22.059481806247806	30.997427267945447	26.683617258254372	20.259473667552378
38	22.662348134204073	28.429216710851748	25.20569045526958	23.702744699674604
39	23.31058015333905	28.124919931815818	26.805094823337562	21.75940509150757
40	24.508626263570143	27.98430136082002	27.070205291056325	20.43686708455351
41	19.981100140618572	30.003602126309538	26.37977262896441	23.63552510410748
42	21.24289936503104	28.895684532107907	26.85483129539395	23.006584807467103
43	22.62949191933046	27.98098559601626	25.710591004932954	23.67893147972033
44	22.620448924411114	26.877137349528336	26.66899774980143	23.833415976259122
45	21.285100007987978	27.421374927090852	26.022122179904354	25.271402885016812
46	22.42828528237505	29.806766270231815	25.587304840865897	22.177643606527234
47	21.34342732521775	27.84714927120997	28.75536739427609	22.0540560092962
48	22.243808186020434	26.480451305733112	27.217153958495665	24.05858654975079
49	21.072740343965382	26.554754580653718	29.012489883149435	23.360015192231465
50	20.94719343116849	26.555508163563662	28.178273601839948	24.319024803427897
51	20.75171402432867	26.8197143317905	26.31195016706933	26.116621476811503
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	128.0
1	477.5
2	827.0
3	2511.0
4	4195.0
5	3025.5
6	1856.0
7	1777.5
8	1699.0
9	1636.0
10	1573.0
11	1679.5
12	1786.0
13	1672.0
14	1558.0
15	1630.5
16	1703.0
17	1667.0
18	1631.0
19	1697.0
20	1763.0
21	1983.5
22	2204.0
23	2434.0
24	2664.0
25	3370.5
26	4865.0
27	5653.0
28	6527.5
29	7402.0
30	9166.5
31	10931.0
32	13199.0
33	15467.0
34	17808.5
35	20150.0
36	21530.5
37	22911.0
38	25171.5
39	27432.0
40	30718.5
41	34005.0
42	36610.0
43	39215.0
44	43232.0
45	47249.0
46	58467.0
47	69685.0
48	62910.0
49	56135.0
50	53594.0
51	51053.0
52	46165.0
53	41277.0
54	38346.5
55	35416.0
56	33931.0
57	32446.0
58	31471.5
59	30497.0
60	29015.5
61	27534.0
62	24456.0
63	21378.0
64	17605.0
65	13832.0
66	11282.5
67	8733.0
68	7373.0
69	6013.0
70	5046.5
71	4080.0
72	3438.0
73	2796.0
74	2443.5
75	1668.5
76	1246.0
77	957.0
78	668.0
79	461.5
80	255.0
81	203.5
82	152.0
83	103.5
84	55.0
85	45.5
86	36.0
87	29.5
88	23.0
89	15.0
90	7.0
91	6.5
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	663497.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.040435769463176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.18305217179993	23.026742661214918
2	8.55133580237535	5.308743794334857
3	3.656703675994137	3.405170267479677
4	2.199803213629975	2.7313140143255974
5	1.492985717015041	2.317146362686565
6	1.086273990902893	2.02310508255958
7	0.8556007410013167	1.8590753891749527
8	0.6746590609561757	1.6753368998317208
9	0.5126707025352504	1.43221698116379
>10	6.482242794940387	46.08451195504756
>50	0.2685735353844957	5.033955803259316
>100	0.030804133090156418	1.814339623489544
>500	0.0028878874772021645	0.6216623166452563
>1k	0.0019252583181347761	1.8847080327997856
>5k	4.8131457953369403E-4	0.7819708159868473
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGC	5234	0.7888505901307769	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCG	4941	0.7446906316079802	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTC	3879	0.584629621535591	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2093	0.3154498061031173	No Hit
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	1702	0.25651962254539207	Illumina PCR Primer Index 5 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCT	936	0.14107072074176671	No Hit
GAACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCT	720	0.10851593903212825	Illumina PCR Primer Index 5 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	698	0.10520017422836878	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTC	688	0.10369300840847812	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0143316397813406E-4	0.0	0.0	0.06932962771497082	0.0
2	3.0143316397813406E-4	0.0	0.0	0.5029412340975167	0.0
3	3.0143316397813406E-4	0.0	0.0	0.6221580504508687	0.0
4	3.0143316397813406E-4	0.0	0.0	0.9082181230661178	0.0
5	3.0143316397813406E-4	0.0	0.0	2.019451482071509	0.0
6	3.0143316397813406E-4	0.0	0.0	2.3972979531181	0.0
7	3.0143316397813406E-4	0.0	0.0	2.820811548507378	0.0
8	3.0143316397813406E-4	0.0	0.0	3.452012593877591	0.0
9	3.0143316397813406E-4	0.0	0.0	3.6301595937886684	0.0
10	3.0143316397813406E-4	0.0	0.0	4.924061450164809	0.0
11	3.0143316397813406E-4	0.0	0.0	5.589324443064551	0.0
12	3.0143316397813406E-4	0.0	0.0	7.0018402494660865	0.0
13	3.0143316397813406E-4	0.0	0.0	7.255948406699654	0.0
14	3.0143316397813406E-4	0.0	0.0	7.384961800882295	0.0
15	3.0143316397813406E-4	0.0	0.0	7.65384018315079	0.0
16	3.0143316397813406E-4	0.0	0.0	8.045251146576398	0.0
17	3.0143316397813406E-4	0.0	0.0	8.508101769864822	0.0
18	3.0143316397813406E-4	0.0	0.0	9.001698575879017	0.0
19	3.0143316397813406E-4	0.0	0.0	9.338550136624582	0.0
20	3.0143316397813406E-4	0.0	0.0	9.651889910579852	0.0
21	3.0143316397813406E-4	0.0	0.0	10.03817651021783	0.0
22	3.0143316397813406E-4	0.0	0.0	10.465458020156836	0.0
23	3.0143316397813406E-4	0.0	0.0	10.90102894210524	0.0
24	3.0143316397813406E-4	0.0	0.0	11.249334963081973	0.0
25	3.0143316397813406E-4	0.0	0.0	11.578047828400129	0.0
26	3.0143316397813406E-4	0.0	0.0	11.878425976304339	0.0
27	3.0143316397813406E-4	0.0	0.0	12.18679210305397	0.0
28	3.0143316397813406E-4	0.0	0.0	12.498021844861393	0.0
29	3.0143316397813406E-4	0.0	0.0	12.825528977523636	0.0
30	3.0143316397813406E-4	0.0	0.0	13.212117010325594	0.0
31	3.0143316397813406E-4	0.0	0.0	13.569013876475704	0.0
32	3.0143316397813406E-4	0.0	0.0	13.92169067833012	0.0
33	3.0143316397813406E-4	0.0	0.0	14.259295821985631	0.0
34	3.0143316397813406E-4	0.0	0.0	14.598860281207	0.0
35	3.0143316397813406E-4	0.0	0.0	14.944453403707929	0.0
36	3.0143316397813406E-4	0.0	0.0	15.308584665793516	0.0
37	3.0143316397813406E-4	0.0	0.0	15.673921660535013	0.0
38	3.0143316397813406E-4	0.0	0.0	16.03639504021872	0.0
39	3.0143316397813406E-4	0.0	0.0	16.379275264243848	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGTA	25	3.8890168E-5	45.0	43
GGTCGAT	20	7.0311275E-4	45.0	1
CCGTGAC	25	3.8890168E-5	45.0	31
TGACCGA	20	7.0311275E-4	45.0	26
CTCGCGG	25	3.8890168E-5	45.0	2
GCGATAT	55	1.8189894E-12	45.0	9
TTTGCGC	20	7.0311275E-4	45.0	13
AATAGCG	25	3.8890168E-5	45.0	1
TAGTCCG	20	7.0311275E-4	45.0	1
ATTTCGG	20	7.0311275E-4	45.0	2
TACGGCT	610	0.0	42.786884	7
TACGGGT	90	0.0	42.500004	4
ACGGCTG	695	0.0	41.43885	8
TATACGG	60	3.6379788E-12	41.249996	2
GCGTATG	55	6.002665E-11	40.909092	1
CGTTTTT	1320	0.0	40.56818	1
TGCGGGT	95	0.0	40.263157	4
TCGATCA	45	1.9261279E-8	40.000004	17
TTGTCGC	40	3.4552795E-7	39.375	38
CAAACGG	35	6.244296E-6	38.571426	2
>>END_MODULE