FastQCFastQC Report
Sat 14 Jan 2017
SRR2934643.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934643.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1475316
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113100.7666154234075954No Hit
GAATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC29570.20043163634096017No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCG21660.14681600416453153No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGC20830.14119009080088604No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC253.891345E-545.00000427
TAACGCG207.0339383E-445.01
TCGATAC207.0339383E-445.039
CCGGTAT207.0339383E-445.045
CGTTTTT70700.043.2178231
TCGATTG356.2494946E-638.5714261
TAGTCCG356.2494946E-638.5714261
CACGACC4150.037.95180527
ACGACCA4200.037.49999628
CCCGTAC250.002107301536.00000443
TTGCGTA250.002107301536.0000041
TACGGCT3250.036.07
CGCGAGG2200.035.7954562
CGTGCGG1900.035.5263142
TCAAGCG4250.035.4705917
ACGGGCT1400.035.3571435
TCGTGCG702.1827873E-1135.3571431
GCTACGA2600.034.61538310
TATACGG653.6743586E-1034.6153832
CGTTTTA4250.034.4117621
Produced by FastQC (version 0.11.5)