FastQCFastQC Report
Sat 14 Jan 2017
SRR2934640.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934640.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582062
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115830.7321457692555665No Hit
GAATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC42900.2711650997242839No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCG27790.17565683266521792No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGC27120.17142185325227458No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT16960.10720186693062599No Hit
GCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC16600.10492635560426836No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT63300.042.0853041
ATTAGCG603.6379788E-1241.2500041
TACGAAT2750.040.90909212
CGAATAT2750.040.90909214
CGTTTTA4000.037.68751
GTTAACG601.5643309E-1037.5000041
CCGACTA301.14011615E-437.50000443
CACGACC6850.037.11678727
GCTACGA3350.036.26865810
CGCACTA250.002107347836.012
TAATCCG250.002107347836.024
CGGGATA2700.035.8333326
AACACGT6550.035.3816841
AGGCGAT3400.035.073537
CGGGTAT1350.035.06
ATTCGAC458.702682E-735.09
GGGCGAT23800.034.8844577
ATAACGG2200.034.7727282
GCGAGAC7350.034.59183521
TCAAGCG7350.034.59183517
Produced by FastQC (version 0.11.5)