##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934640.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1582062
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.169580585337364	31.0	31.0	33.0	28.0	34.0
2	31.22945055250679	31.0	31.0	34.0	28.0	34.0
3	30.970553619263974	31.0	31.0	34.0	27.0	34.0
4	34.934149230561125	35.0	35.0	37.0	32.0	37.0
5	35.180001795125605	35.0	35.0	37.0	33.0	37.0
6	35.16081291377961	36.0	35.0	37.0	32.0	37.0
7	31.027383250466794	35.0	35.0	37.0	0.0	37.0
8	33.08006007349901	36.0	35.0	37.0	17.0	37.0
9	36.470937927843536	39.0	35.0	39.0	32.0	39.0
10	36.146986654126074	37.0	35.0	39.0	32.0	39.0
11	36.36364314420042	38.0	35.0	39.0	32.0	39.0
12	36.40022957380937	38.0	35.0	39.0	32.0	39.0
13	36.364575471757746	38.0	35.0	39.0	32.0	39.0
14	37.375966302205605	39.0	36.0	41.0	32.0	41.0
15	37.56751505313951	39.0	36.0	41.0	32.0	41.0
16	37.48431477401012	39.0	36.0	41.0	32.0	41.0
17	37.39920116910715	39.0	36.0	41.0	32.0	41.0
18	37.303348414916734	39.0	36.0	40.0	32.0	41.0
19	37.06280474469395	39.0	35.0	40.0	32.0	41.0
20	37.020164822870406	39.0	35.0	40.0	32.0	41.0
21	37.007389090945864	39.0	35.0	40.0	31.0	41.0
22	37.10537134448587	39.0	35.0	40.0	32.0	41.0
23	37.14440963754897	39.0	35.0	40.0	32.0	41.0
24	37.081955068764685	39.0	35.0	40.0	32.0	41.0
25	36.71995914192996	39.0	35.0	40.0	31.0	41.0
26	36.71787957741226	39.0	35.0	40.0	31.0	41.0
27	36.73013194173174	39.0	35.0	40.0	31.0	41.0
28	36.69983098007537	39.0	35.0	40.0	31.0	41.0
29	36.63339553064292	39.0	35.0	40.0	31.0	41.0
30	36.3759448112653	38.0	35.0	40.0	30.0	41.0
31	36.331087530071514	38.0	35.0	40.0	30.0	41.0
32	36.1612737048232	38.0	35.0	40.0	30.0	41.0
33	35.96716563573362	38.0	35.0	40.0	29.0	41.0
34	35.81401550634552	38.0	35.0	40.0	29.0	41.0
35	35.696583319743475	38.0	35.0	40.0	28.0	41.0
36	35.59807769859841	38.0	35.0	40.0	27.0	41.0
37	35.50216805662483	38.0	34.0	40.0	27.0	41.0
38	35.34838773701663	38.0	34.0	40.0	26.0	41.0
39	35.25053253285902	38.0	34.0	40.0	26.0	41.0
40	35.12516323633334	38.0	34.0	40.0	25.0	41.0
41	34.940762119310115	38.0	34.0	40.0	24.0	41.0
42	34.92800914249884	38.0	34.0	40.0	25.0	41.0
43	34.89270584844336	38.0	34.0	40.0	25.0	41.0
44	34.70107176583471	37.0	34.0	40.0	24.0	41.0
45	34.605636188720794	37.0	34.0	40.0	24.0	41.0
46	34.51298874506814	37.0	33.0	40.0	24.0	41.0
47	34.45015998108797	37.0	33.0	40.0	23.0	41.0
48	34.36245861413775	37.0	33.0	40.0	23.0	41.0
49	34.248136293015065	36.0	33.0	40.0	24.0	41.0
50	34.08976828973833	36.0	33.0	40.0	23.0	41.0
51	32.455313382155694	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	8.0
8	70.0
9	139.0
10	155.0
11	97.0
12	74.0
13	55.0
14	88.0
15	163.0
16	305.0
17	586.0
18	1112.0
19	1921.0
20	2987.0
21	4638.0
22	6820.0
23	9488.0
24	13660.0
25	18654.0
26	23897.0
27	26722.0
28	28953.0
29	32413.0
30	38434.0
31	47638.0
32	61851.0
33	84694.0
34	126209.0
35	136970.0
36	164036.0
37	235587.0
38	292047.0
39	221585.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.726216798077445	20.60197387965832	24.562311717239908	14.109497605024329
2	34.451367898350384	23.41570684334748	24.902753495122187	17.230171763179953
3	31.841356407018186	23.042839029064602	29.21219269535581	15.90361186856141
4	28.37948196720482	25.649437253407264	27.397535621233555	18.57354515815436
5	25.343128145420344	29.09658407824725	26.59345841060591	18.9668293657265
6	25.98172511570343	33.8192814188066	25.682811419527173	14.5161820459628
7	70.52808297019965	16.855218063514577	9.336549389341252	3.2801495769445195
8	83.07809681289355	4.438511259356461	9.282253160748441	3.201138767001546
9	77.7180666750102	5.891678075827622	10.998241535413909	5.39201371374826
10	43.732230468843824	24.33425491542051	17.590271430576045	14.34324318515962
11	32.09722501393751	24.83404569479578	24.59119806935506	18.477531221911658
12	27.560108263772214	21.980870534783087	29.95363013586067	20.50539106558403
13	24.975127397029954	22.519850675890073	32.29942947874356	20.20559244833641
14	21.57987487216051	24.258214911931393	32.66231032664965	21.49959988925845
15	21.23589340999278	23.317607021722285	34.26306933609429	21.18343023219065
16	25.567582054306342	23.491304386300914	29.926703251832105	21.014410307560638
17	26.268123499584718	22.566372240784496	28.60273491177969	22.562769347851095
18	27.339446873763478	21.878346107801086	29.303276357058067	21.478930661377365
19	26.125019120616006	24.64479900282037	27.411504732431474	21.818677144132153
20	27.35000271797186	25.033152935852073	27.442097718041392	20.174746628134674
21	27.097926629929802	24.808888652909936	28.50728985336858	19.585894863791683
22	26.42361677355249	21.488095915330753	29.099112424165423	22.989174886951332
23	24.164539695663002	24.303725138458542	29.44840341276132	22.083331753117136
24	24.443605876381582	23.05036085817117	30.019683172972993	22.486350092474254
25	24.80515934268063	25.27043820027281	27.793474592019784	22.13092786502678
26	23.95487661039833	24.4618731756404	27.237870576500793	24.34537963746048
27	23.820684650791183	23.82150636321459	28.526631699642618	23.83117728635161
28	22.490901115126967	24.633168611596766	29.153282235462324	23.722648037813943
29	23.343459358735625	24.68392515590413	28.531056304999424	23.441559180360823
30	24.674001398175292	24.140141157552613	28.95303723874286	22.23282020552924
31	25.781732953575776	24.802504579466543	27.26764184968731	22.148120617270372
32	25.480859789312937	24.54101040287928	27.4725642863554	22.505565521452382
33	24.841757149846213	24.03963940730515	26.665010600090262	24.453592842758376
34	23.05895723429297	25.348500880496466	28.75051673069703	22.84202515451354
35	23.594334482466554	26.38183585725465	27.551701513594285	22.47212814668452
36	25.070762081384927	27.15443516120101	25.96067663593462	21.814126121479436
37	24.040587537024464	26.493019869006396	26.64276115601032	22.823631437958817
38	24.381029314906748	26.347576770063373	26.83030121449096	22.441092700538917
39	24.57261472685647	24.458649534594727	26.628855253460358	24.339880485088447
40	26.138040102094607	23.826499846402985	27.559349759996763	22.476110291505645
41	22.826602244412673	24.680511888914594	27.41953223072168	25.073353635951058
42	23.502239482397023	24.753454668653944	27.938728065018942	23.805577783930087
43	23.162872251529965	24.242349541294843	28.460578662530295	24.1341995446449
44	23.533717389078305	24.140014740256703	28.198894859999164	24.127373010665828
45	23.31223428664616	24.326543460370072	27.168277855103025	25.19294439788074
46	23.612412155781506	24.798332808701556	27.33755061432485	24.251704421192088
47	22.62850634172365	24.947442009225934	29.239562039920052	23.184489609130363
48	22.857258438670545	25.468660520257743	28.28884076603825	23.38524027503347
49	23.513553830380857	24.521668556605242	28.763853755415404	23.2009238575985
50	22.20292251504682	25.640967294581372	28.540727228136443	23.615382962235362
51	22.62679970822888	24.140520409440338	27.85390205946417	25.37877782286661
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	488.0
1	1312.0
2	2136.0
3	11665.5
4	21195.0
5	14385.5
6	7576.0
7	7137.0
8	6698.0
9	6570.0
10	6442.0
11	6389.0
12	6336.0
13	6116.5
14	5897.0
15	5613.0
16	5329.0
17	5028.5
18	4728.0
19	4745.0
20	4762.0
21	4975.0
22	5188.0
23	5949.5
24	6711.0
25	7337.0
26	9585.0
27	11207.0
28	13075.5
29	14944.0
30	17475.5
31	20007.0
32	24013.0
33	28019.0
34	32742.5
35	37466.0
36	40481.0
37	43496.0
38	47317.5
39	51139.0
40	55457.0
41	59775.0
42	65340.0
43	70905.0
44	77697.0
45	84489.0
46	98587.0
47	112685.0
48	112752.5
49	112820.0
50	115040.0
51	117260.0
52	110651.5
53	104043.0
54	101623.0
55	99203.0
56	98526.0
57	97849.0
58	93512.5
59	89176.0
60	84891.0
61	80606.0
62	75980.0
63	71354.0
64	63762.0
65	56170.0
66	49699.5
67	43229.0
68	36961.5
69	30694.0
70	25964.5
71	21235.0
72	17374.5
73	13514.0
74	11177.5
75	6969.5
76	5098.0
77	4015.5
78	2933.0
79	2081.0
80	1229.0
81	915.0
82	601.0
83	445.5
84	290.0
85	214.5
86	139.0
87	121.5
88	104.0
89	68.5
90	33.0
91	31.5
92	30.0
93	23.0
94	16.0
95	14.0
96	12.0
97	6.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1582062.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.043594351963026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.78752157655916	20.412237289777813
2	8.340412192660756	4.677902725182895
3	3.7659123052331385	3.1682915115907235
4	2.4307272471246577	2.7266531559451077
5	1.688001140085036	2.3668809619097937
6	1.3191162338127183	2.219565633847985
7	0.9725613614443227	1.9091881411916236
8	0.7598765317566917	1.704773537132897
9	0.5747601820695721	1.4506507256057544
>10	6.817102472354211	47.09270591400668
>50	0.5090887316394443	8.518501401348404
>100	0.030694036452087927	1.591428279727851
>500	0.002446625099546656	0.45745599528182807
>1k	0.0015569432451660538	0.981278663454284
>5k	0.0	0.0
>10k+	2.2242046359515052E-4	0.722486063996375
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11583	0.7321457692555665	No Hit
GAATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC	4290	0.2711650997242839	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCG	2779	0.17565683266521792	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGC	2712	0.17142185325227458	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1696	0.10720186693062599	No Hit
GCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC	1660	0.10492635560426836	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.528345918175141E-4	0.0	0.0	0.04860745027691709	0.0
2	2.528345918175141E-4	0.0	0.0	0.24246837355299602	0.0
3	2.528345918175141E-4	0.0	0.0	0.3590883290288244	0.0
4	2.528345918175141E-4	0.0	0.0	0.5751354877368902	0.0
5	2.528345918175141E-4	0.0	0.0	1.194453820393891	0.0
6	2.528345918175141E-4	0.0	0.0	1.7066334947682202	0.0
7	2.528345918175141E-4	0.0	0.0	2.067744500531585	0.0
8	2.528345918175141E-4	0.0	0.0	2.6189239106937654	0.0
9	2.528345918175141E-4	0.0	0.0	2.9076610145493667	0.0
10	2.528345918175141E-4	0.0	0.0	3.5488495393985824	0.0
11	2.528345918175141E-4	0.0	0.0	4.06678120073676	0.0
12	2.528345918175141E-4	0.0	0.0	4.6414742279379695	0.0
13	2.528345918175141E-4	0.0	0.0	4.87332354863463	0.0
14	2.528345918175141E-4	0.0	0.0	5.017565683266522	0.0
15	2.528345918175141E-4	0.0	0.0	5.164905041648178	0.0
16	2.528345918175141E-4	0.0	0.0	5.393151469411439	0.0
17	2.528345918175141E-4	0.0	0.0	5.651169170361213	0.0
18	2.528345918175141E-4	0.0	0.0	5.9742917787039955	0.0
19	2.528345918175141E-4	0.0	0.0	6.177381164581413	0.0
20	3.160432397718926E-4	0.0	0.0	6.385906494182908	0.0
21	3.160432397718926E-4	0.0	0.0	6.633621185516118	0.0
22	3.160432397718926E-4	0.0	0.0	6.898655046388827	0.0
23	3.160432397718926E-4	0.0	0.0	7.16071810080768	0.0
24	3.160432397718926E-4	0.0	0.0	7.390355118825937	0.0
25	3.792518877262712E-4	0.0	0.0	7.594961512254261	0.0
26	3.792518877262712E-4	0.0	0.0	7.791097946856697	0.0
27	3.792518877262712E-4	0.0	0.0	8.00840927852385	0.0
28	3.792518877262712E-4	0.0	0.0	8.221232796186243	0.0
29	3.792518877262712E-4	0.0	0.0	8.450490562316775	0.0
30	3.792518877262712E-4	0.0	0.0	8.776583977113413	0.0
31	4.424605356806497E-4	0.0	0.0	9.011972982095518	0.0
32	4.424605356806497E-4	0.0	0.0	9.257475370750324	0.0
33	4.424605356806497E-4	0.0	0.0	9.507275947466029	0.0
34	4.424605356806497E-4	0.0	0.0	9.756697272294007	0.0
35	4.424605356806497E-4	0.0	0.0	10.043790951302794	0.0
36	4.424605356806497E-4	0.0	0.0	10.29378115396236	0.0
37	4.424605356806497E-4	0.0	0.0	10.552114898151906	0.0
38	4.424605356806497E-4	0.0	0.0	10.866830756316757	0.0
39	4.424605356806497E-4	0.0	0.0	11.386405842501748	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	6330	0.0	42.085304	1
ATTAGCG	60	3.6379788E-12	41.250004	1
TACGAAT	275	0.0	40.909092	12
CGAATAT	275	0.0	40.909092	14
CGTTTTA	400	0.0	37.6875	1
GTTAACG	60	1.5643309E-10	37.500004	1
CCGACTA	30	1.14011615E-4	37.500004	43
CACGACC	685	0.0	37.116787	27
GCTACGA	335	0.0	36.268658	10
CGCACTA	25	0.0021073478	36.0	12
TAATCCG	25	0.0021073478	36.0	24
CGGGATA	270	0.0	35.833332	6
AACACGT	655	0.0	35.38168	41
AGGCGAT	340	0.0	35.07353	7
CGGGTAT	135	0.0	35.0	6
ATTCGAC	45	8.702682E-7	35.0	9
GGGCGAT	2380	0.0	34.884457	7
ATAACGG	220	0.0	34.772728	2
GCGAGAC	735	0.0	34.591835	21
TCAAGCG	735	0.0	34.591835	17
>>END_MODULE