FastQCFastQC Report
Sat 14 Jan 2017
SRR2934637.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934637.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974980
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGC57640.5911916141869576No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCG52700.5405239081827319No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC46730.47929188291041863RNA PCR Primer, Index 4 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40100.41129048801001045No Hit
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC14110.1447209173521508RNA PCR Primer, Index 48 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCT10350.10615602371330694TruSeq Adapter, Index 4 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTA207.03276E-445.034
ACCGTTC207.03276E-445.023
TAGTACG253.8903694E-545.01
TTGCGAA403.4574987E-739.3751
TACGAAT1100.038.86363612
TACGGCT7300.038.8356177
CGTGAAT356.2473155E-638.57142625
CGTTTTT21650.038.036951
AACCGAC301.13979506E-437.49999627
CGAATAT1150.037.17391614
TGGGCGA4250.036.529416
GTTACGA250.002106952536.043
TGCGAAA250.002106952536.032
CCGACCA250.002106952536.018
CTCGACC250.002106952536.024
ATACGAT250.002106952536.011
TGCGTAA250.002106952536.01
CTTACGG504.87762E-836.02
TGATACC8650.035.6358384
TACGGGA2400.035.6254
Produced by FastQC (version 0.11.5)