##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934637.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	974980
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.164688506430902	31.0	31.0	33.0	28.0	34.0
2	31.21058790949558	31.0	31.0	34.0	28.0	34.0
3	30.99813534636608	31.0	31.0	34.0	28.0	34.0
4	34.96079201624649	35.0	35.0	37.0	32.0	37.0
5	35.174303062626926	35.0	35.0	37.0	33.0	37.0
6	35.12125376930809	36.0	35.0	37.0	32.0	37.0
7	30.912990010051487	35.0	35.0	37.0	0.0	37.0
8	32.98608176577981	35.0	35.0	37.0	17.0	37.0
9	36.34637325893865	39.0	35.0	39.0	32.0	39.0
10	36.09493117807545	37.0	35.0	39.0	32.0	39.0
11	36.28056370387085	37.0	35.0	39.0	32.0	39.0
12	36.2921126587212	38.0	35.0	39.0	32.0	39.0
13	36.183956593981414	38.0	35.0	39.0	32.0	39.0
14	37.125656936552545	39.0	36.0	40.0	31.0	41.0
15	37.31680957558104	39.0	36.0	41.0	32.0	41.0
16	37.32584770969661	39.0	36.0	40.0	32.0	41.0
17	37.24155059590966	39.0	36.0	40.0	32.0	41.0
18	37.17980061129459	39.0	36.0	40.0	32.0	41.0
19	36.98010830991405	39.0	35.0	40.0	31.0	41.0
20	36.934209932511436	39.0	35.0	40.0	31.0	41.0
21	36.951797985599704	39.0	35.0	40.0	31.0	41.0
22	37.08188988492071	39.0	35.0	40.0	32.0	41.0
23	37.144105520113236	39.0	35.0	40.0	32.0	41.0
24	37.05529139059263	39.0	35.0	40.0	32.0	41.0
25	36.76423413813617	39.0	35.0	40.0	31.0	41.0
26	36.725848735358674	39.0	35.0	40.0	31.0	41.0
27	36.757340663398224	39.0	35.0	40.0	31.0	41.0
28	36.743990645961965	39.0	35.0	40.0	31.0	41.0
29	36.68961927424152	39.0	35.0	40.0	31.0	41.0
30	36.43631459106854	38.0	35.0	40.0	30.0	41.0
31	36.414824919485525	38.0	35.0	40.0	30.0	41.0
32	36.27042708568381	38.0	35.0	40.0	30.0	41.0
33	36.1199983589407	38.0	35.0	40.0	30.0	41.0
34	36.02100658475046	38.0	35.0	40.0	29.0	41.0
35	35.90969353217502	38.0	35.0	40.0	29.0	41.0
36	35.839876715419805	38.0	35.0	40.0	29.0	41.0
37	35.708210424829225	38.0	35.0	40.0	28.0	41.0
38	35.556492440870585	38.0	34.0	40.0	28.0	41.0
39	35.45495189644916	38.0	34.0	40.0	27.0	41.0
40	35.300795913762336	38.0	34.0	40.0	26.0	41.0
41	35.11755010359187	38.0	34.0	40.0	26.0	41.0
42	35.148454327268254	38.0	34.0	40.0	26.0	41.0
43	35.11595417341894	38.0	34.0	40.0	26.0	41.0
44	34.9343801924142	38.0	34.0	40.0	26.0	41.0
45	34.818163449506656	37.0	34.0	40.0	25.0	41.0
46	34.72748876900039	37.0	34.0	40.0	25.0	41.0
47	34.628781103202115	37.0	33.0	40.0	24.0	41.0
48	34.557353997005066	37.0	33.0	40.0	24.0	41.0
49	34.4823165603397	37.0	33.0	40.0	24.0	41.0
50	34.32992061375618	36.0	33.0	40.0	24.0	41.0
51	32.62066811626905	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	7.0
8	55.0
9	93.0
10	84.0
11	71.0
12	49.0
13	42.0
14	69.0
15	121.0
16	207.0
17	359.0
18	756.0
19	1161.0
20	1811.0
21	2772.0
22	4006.0
23	5496.0
24	7633.0
25	10387.0
26	12901.0
27	15012.0
28	17010.0
29	20030.0
30	24185.0
31	30677.0
32	39548.0
33	54274.0
34	79286.0
35	84752.0
36	99930.0
37	143436.0
38	180331.0
39	138422.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49555888325914	20.603704691378287	23.580688834642764	12.32004759071981
2	32.816057765287496	24.599273831258078	25.579088801821577	17.005579601632853
3	32.8317503948799	24.32049888202835	26.96957886315617	15.87817185993559
4	28.5519702968266	25.72155326263103	27.05378571868141	18.67269072186096
5	26.802908777615954	29.489733122730723	24.86953578534944	18.837822314303885
6	25.982686824345112	35.793452173377915	24.637633592483947	13.586227409793022
7	70.28780077540053	17.27696978399557	9.155162157172454	3.280067283431455
8	82.62497692260354	5.941250076924655	8.076883628382122	3.3568893720896837
9	77.17542923957414	5.964532605797042	9.652300560011486	7.207737594617325
10	45.14420808631972	24.131982194506556	16.46208127346202	14.261728445711707
11	33.91433670434265	24.50286159716097	23.653613407454515	17.929188291041868
12	29.50881043713717	22.612771544031673	27.922829186239717	19.955588832591438
13	26.025251800036926	22.69646556852448	31.780651910808427	19.497630720630166
14	22.358202219532707	25.38339247984574	30.965865966481367	21.292539334140187
15	21.061047406100638	24.222548154833945	34.41516749061519	20.301236948450224
16	26.684034544298346	23.72058914029006	28.60971507107838	20.98566124433322
17	27.042298303554947	23.109397115838274	28.17329586247923	21.67500871812755
18	27.205276005661656	22.783339145418367	28.588073601509773	21.423311247410204
19	26.585776118484482	25.358468891669574	26.39438757718107	21.661367412664877
20	26.972143018318324	25.908121192229583	27.720363494635787	19.399372294816303
21	26.182690926993374	25.938378223143037	28.435455086258184	19.44347576360541
22	25.54411372540975	21.79470348109705	29.68337812057683	22.97780467291637
23	23.696588647972266	26.04514964409526	28.91638802847238	21.34187367946009
24	23.692485999712815	24.767379843689103	28.651869781944246	22.88826437465384
25	25.024410757143734	26.1645367084453	26.473158423762538	22.337894110648424
26	23.48355863710025	24.82758620689655	27.27307226814909	24.41578288785411
27	23.323452788775153	25.00933352479025	28.365607499641015	23.301606186793574
28	21.853576483620177	25.67406510902788	28.88602843135244	23.586329975999508
29	24.723891772138913	25.634577119530654	27.27943137295124	22.362099735379186
30	25.034769943998853	25.686270487599742	27.942214199265624	21.336745369135777
31	26.831217050606167	24.267472153274937	26.624341012123327	22.276969783995572
32	26.952758005292416	25.452111838191556	25.948839976204642	21.646290180311393
33	25.463394120905043	25.830786272538926	25.437034605838065	23.268785000717966
34	24.721430183183244	26.009456604238036	27.732568873207658	21.536544339371062
35	23.64735686885885	27.578719563478227	27.05040103386736	21.723522533795563
36	26.500338468481406	26.708958132474514	25.12933598637921	21.661367412664877
37	24.329114443373197	28.081191409054547	25.722784057108868	21.866910090463392
38	25.020820939916717	27.38661305872941	25.20913249502554	22.383433506328334
39	23.80069334755585	25.796221460953046	25.52842109581735	24.874664095673758
40	25.02892367022913	25.375187183326837	27.819750148721	21.77613899772303
41	22.905290364930565	25.61149972307124	26.797472768672176	24.685737143326016
42	22.922418921413772	24.428398531251926	29.315165439291064	23.33401710804324
43	23.41391618289606	24.248805103694433	27.335637654105728	25.00164105930378
44	24.114238240784427	24.23013805411393	27.88128987261277	23.77433383248887
45	22.995343494225523	24.094135264313113	27.052554924203577	25.85796631725779
46	23.972594309626864	25.651397977394407	26.857884264292604	23.518123448686126
47	21.68629100084104	25.442573180988326	30.20769656813473	22.6634392500359
48	22.869392192660364	26.144228599561014	27.703132371946094	23.28324683583253
49	23.16139818252682	24.739994666557262	28.776385156618595	23.32222199429732
50	22.500256415516215	25.712732568873207	28.01247205070873	23.774538964901843
51	22.254097519949127	26.11181767831135	26.504030851914912	25.13005394982461
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	281.0
1	788.0
2	1295.0
3	4520.5
4	7746.0
5	5359.0
6	2972.0
7	2813.0
8	2654.0
9	2623.5
10	2593.0
11	2637.0
12	2681.0
13	2736.5
14	2792.0
15	2783.5
16	2775.0
17	2706.5
18	2638.0
19	2647.5
20	2657.0
21	2769.5
22	2882.0
23	3330.0
24	3778.0
25	4687.5
26	6547.0
27	7497.0
28	8690.5
29	9884.0
30	12041.5
31	14199.0
32	15844.5
33	17490.0
34	20753.5
35	24017.0
36	25006.0
37	25995.0
38	29074.0
39	32153.0
40	35014.5
41	37876.0
42	42613.5
43	47351.0
44	52434.0
45	57517.0
46	72008.5
47	86500.0
48	80013.5
49	73527.0
50	73663.5
51	73800.0
52	70540.5
53	67281.0
54	64391.5
55	61502.0
56	59177.5
57	56853.0
58	55017.0
59	53181.0
60	50789.5
61	48398.0
62	43768.5
63	39139.0
64	35121.5
65	31104.0
66	26727.0
67	22350.0
68	19375.5
69	16401.0
70	13398.0
71	10395.0
72	8904.0
73	7413.0
74	5935.0
75	3510.5
76	2564.0
77	2007.5
78	1451.0
79	1112.0
80	773.0
81	536.0
82	299.0
83	211.5
84	124.0
85	94.5
86	65.0
87	49.5
88	34.0
89	29.0
90	24.0
91	18.0
92	12.0
93	8.5
94	5.0
95	4.5
96	4.0
97	3.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	974980.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.386737918974703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.4252167420645	21.57727601042963
2	8.45524628906944	4.969442134745341
3	3.678575506528239	3.243040029765149
4	2.404085226528622	2.8259288994750213
5	1.6181439572612144	2.3775986193603953
6	1.1821187471346406	2.0843168286691443
7	0.8933304857198991	1.8376448201216737
8	0.6765968833320088	1.5906380229818278
9	0.536267823126226	1.4183245775330537
>10	6.710411307663897	47.18982926349517
>50	0.39022660429796224	6.830396783910478
>100	0.025672782132257255	1.3956121504078465
>500	0.0020538225705798202	0.43053337204442255
>1k	0.0013692150470532133	1.1194873592248549
>5k	6.846075235266067E-4	1.1099311278360184
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGC	5764	0.5911916141869576	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCG	5270	0.5405239081827319	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC	4673	0.47929188291041863	RNA PCR Primer, Index 4 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4010	0.41129048801001045	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	1411	0.1447209173521508	RNA PCR Primer, Index 48 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCT	1035	0.10615602371330694	TruSeq Adapter, Index 4 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04738558739666455	0.0
2	0.0	0.0	0.0	0.30328827257995034	0.0
3	0.0	0.0	0.0	0.38636689983384276	0.0
4	0.0	0.0	0.0	0.6108843258323248	0.0
5	1.025662064862869E-4	0.0	0.0	1.4369525528728795	0.0
6	1.025662064862869E-4	0.0	0.0	1.766805472932778	0.0
7	1.025662064862869E-4	0.0	0.0	2.1387105376520545	0.0
8	1.025662064862869E-4	0.0	0.0	2.7301072842519845	0.0
9	1.025662064862869E-4	0.0	0.0	3.004471886602802	0.0
10	1.025662064862869E-4	0.0	0.0	4.067775749246138	0.0
11	1.025662064862869E-4	0.0	0.0	4.61824857945804	0.0
12	1.025662064862869E-4	0.0	0.0	5.699809226855936	0.0
13	1.025662064862869E-4	0.0	0.0	5.90935198670742	0.0
14	1.025662064862869E-4	0.0	0.0	6.028431352437999	0.0
15	1.025662064862869E-4	0.0	0.0	6.2242302406203205	0.0
16	1.025662064862869E-4	0.0	0.0	6.479414962358202	0.0
17	1.025662064862869E-4	0.0	0.0	6.754292395741451	0.0
18	1.025662064862869E-4	0.0	0.0	7.081478594432706	0.0
19	1.025662064862869E-4	0.0	0.0	7.30650885146362	0.0
20	1.025662064862869E-4	0.0	0.0	7.533487866417772	0.0
21	1.025662064862869E-4	0.0	0.0	7.786005866787011	0.0
22	1.025662064862869E-4	0.0	0.0	8.070421957373485	0.0
23	1.025662064862869E-4	0.0	0.0	8.319247574309216	0.0
24	2.051324129725738E-4	0.0	0.0	8.542944470655808	0.0
25	2.051324129725738E-4	0.0	0.0	8.742846007097581	0.0
26	2.051324129725738E-4	0.0	0.0	8.94397833801719	0.0
27	2.051324129725738E-4	0.0	0.0	9.19229112392049	0.0
28	2.051324129725738E-4	0.0	0.0	9.408705819606556	0.0
29	2.051324129725738E-4	0.0	0.0	9.650556934501221	0.0
30	3.076986194588607E-4	0.0	0.0	9.979897023528688	0.0
31	3.076986194588607E-4	0.0	0.0	10.226876448747667	0.0
32	3.076986194588607E-4	0.0	0.0	10.486266384951486	0.0
33	3.076986194588607E-4	0.0	0.0	10.72647644054237	0.0
34	3.076986194588607E-4	0.0	0.0	11.000943609099673	0.0
35	3.076986194588607E-4	0.0	0.0	11.296436849986666	0.0
36	3.076986194588607E-4	0.0	0.0	11.550595909659686	0.0
37	3.076986194588607E-4	0.0	0.0	11.827627233379147	0.0
38	3.076986194588607E-4	0.0	0.0	12.187942316765472	0.0
39	3.076986194588607E-4	0.0	0.0	12.845289134136085	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCGTA	20	7.03276E-4	45.0	34
ACCGTTC	20	7.03276E-4	45.0	23
TAGTACG	25	3.8903694E-5	45.0	1
TTGCGAA	40	3.4574987E-7	39.375	1
TACGAAT	110	0.0	38.863636	12
TACGGCT	730	0.0	38.835617	7
CGTGAAT	35	6.2473155E-6	38.571426	25
CGTTTTT	2165	0.0	38.03695	1
AACCGAC	30	1.13979506E-4	37.499996	27
CGAATAT	115	0.0	37.173916	14
TGGGCGA	425	0.0	36.52941	6
GTTACGA	25	0.0021069525	36.0	43
TGCGAAA	25	0.0021069525	36.0	32
CCGACCA	25	0.0021069525	36.0	18
CTCGACC	25	0.0021069525	36.0	24
ATACGAT	25	0.0021069525	36.0	11
TGCGTAA	25	0.0021069525	36.0	1
CTTACGG	50	4.87762E-8	36.0	2
TGATACC	865	0.0	35.635838	4
TACGGGA	240	0.0	35.625	4
>>END_MODULE