##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934636.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	439619
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.205505221566856	31.0	31.0	33.0	28.0	34.0
2	31.258080292253066	31.0	31.0	34.0	28.0	34.0
3	31.06328661864023	31.0	31.0	34.0	28.0	34.0
4	34.99798234380225	35.0	35.0	37.0	32.0	37.0
5	35.23831772512107	35.0	35.0	37.0	33.0	37.0
6	35.208726192453014	37.0	35.0	37.0	33.0	37.0
7	31.074018638866836	35.0	35.0	37.0	0.0	37.0
8	33.09368339402983	36.0	35.0	37.0	17.0	37.0
9	36.437785900973346	39.0	35.0	39.0	32.0	39.0
10	36.24389073265714	37.0	35.0	39.0	32.0	39.0
11	36.45452084646023	38.0	35.0	39.0	32.0	39.0
12	36.46018939126835	38.0	35.0	39.0	32.0	39.0
13	36.332378718845185	38.0	35.0	39.0	32.0	39.0
14	37.41526640113371	39.0	36.0	41.0	32.0	41.0
15	37.620480461490516	39.0	36.0	41.0	33.0	41.0
16	37.563804112197154	39.0	36.0	41.0	33.0	41.0
17	37.50060848143506	39.0	36.0	41.0	33.0	41.0
18	37.39844274246563	39.0	36.0	40.0	32.0	41.0
19	37.18963238622535	39.0	36.0	40.0	32.0	41.0
20	37.17713065176892	39.0	35.0	40.0	32.0	41.0
21	37.18248983779136	39.0	35.0	40.0	32.0	41.0
22	37.256981613624525	39.0	35.0	40.0	32.0	41.0
23	37.30678610342137	39.0	35.0	40.0	32.0	41.0
24	37.21020474547279	39.0	35.0	41.0	32.0	41.0
25	36.908682290801806	39.0	35.0	40.0	31.0	41.0
26	36.86593846034862	39.0	35.0	40.0	31.0	41.0
27	36.87136133788576	39.0	35.0	40.0	31.0	41.0
28	36.87917492192103	39.0	35.0	40.0	31.0	41.0
29	36.888173623069065	39.0	35.0	40.0	31.0	41.0
30	36.7034045389303	39.0	35.0	40.0	31.0	41.0
31	36.68614641314411	39.0	35.0	40.0	31.0	41.0
32	36.502812662783	39.0	35.0	40.0	31.0	41.0
33	36.35735261669764	39.0	35.0	40.0	30.0	41.0
34	36.23555624301952	39.0	35.0	40.0	30.0	41.0
35	36.164638016100305	39.0	35.0	40.0	30.0	41.0
36	36.0909651311704	38.0	35.0	40.0	30.0	41.0
37	36.017007909121304	38.0	35.0	40.0	30.0	41.0
38	35.93138376639772	38.0	35.0	40.0	29.0	41.0
39	35.886064978993176	38.0	35.0	40.0	29.0	41.0
40	35.75539501249946	38.0	35.0	40.0	29.0	41.0
41	35.630609687024446	38.0	35.0	40.0	28.0	41.0
42	35.60036986572464	38.0	35.0	40.0	28.0	41.0
43	35.530491175313166	38.0	35.0	40.0	28.0	41.0
44	35.34228502407767	38.0	34.0	40.0	27.0	41.0
45	35.2822580461718	38.0	34.0	40.0	27.0	41.0
46	35.173830066489394	38.0	34.0	40.0	27.0	41.0
47	35.136263446302365	38.0	34.0	40.0	27.0	41.0
48	35.065772862410405	37.0	34.0	40.0	26.0	41.0
49	34.96346836692682	37.0	34.0	40.0	26.0	41.0
50	34.805827318655474	37.0	34.0	40.0	26.0	41.0
51	33.20274146476836	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	25.0
9	46.0
10	68.0
11	35.0
12	22.0
13	19.0
14	27.0
15	37.0
16	66.0
17	130.0
18	265.0
19	445.0
20	692.0
21	962.0
22	1489.0
23	2093.0
24	2962.0
25	4022.0
26	5423.0
27	6291.0
28	7065.0
29	7970.0
30	9592.0
31	12319.0
32	15804.0
33	22541.0
34	36040.0
35	39178.0
36	43059.0
37	64482.0
38	86044.0
39	70403.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.39102495570028	21.40876531724061	23.882498254170088	12.317711472889025
2	33.5679304124708	24.04513908634522	25.256187744387752	17.130742756796224
3	33.61774627575242	22.588423157324865	27.56568756127465	16.22814300564807
4	29.406827275436232	26.131718601789277	26.0336791630935	18.427774959680995
5	25.98682040585143	30.666326978588277	23.692106119162272	19.65474649639802
6	26.42606438757197	36.477495285690566	23.48738339334742	13.609056933390049
7	70.65072255748728	17.475814284641928	8.510096242428103	3.36336691544269
8	81.65638882759845	6.198094258892358	8.576972332860954	3.5685445806482434
9	74.97583134486908	8.460280379146488	10.237046169524065	6.326842106460368
10	43.056373814598544	28.07885919398388	15.863964023392985	13.000802968024585
11	35.684990867091734	25.30918818340427	21.89918998041486	17.106630969089142
12	32.99993858318226	22.633917096394832	26.54889802306088	17.817246297362033
13	25.62810069628474	25.394716788855803	28.589756129739612	20.38742638511984
14	20.07101603888822	29.273530033961226	28.869771324715266	21.78568260243529
15	20.665849292228042	25.934047436530268	34.03833774245426	19.361765528787426
16	23.026074851177952	24.17479681269463	32.791121402851104	20.008006933276317
17	23.300858243160555	23.437567530065806	28.8918358851642	24.36973834160944
18	23.878176329958443	25.23526053241557	28.707812901626177	22.178750235999807
19	27.24108830601043	25.985683057374683	26.205646252777974	20.567582383836914
20	30.017583407450545	24.40749831103751	26.857346929955256	18.71757135155669
21	26.34872469115302	26.500446977951363	26.870540172285544	20.28028815861007
22	24.74233370259247	24.832639171646356	26.26911029778058	24.15591682798059
23	25.285986274478585	26.935823974850955	27.051606049784017	20.72658370088645
24	24.000327556361302	23.83882407266292	29.36611019996861	22.794738171007168
25	22.9569240637916	26.66013070408695	27.301822714668837	23.081122517452613
26	22.851605594844628	28.071807633428037	27.40987081996001	21.666715951767326
27	23.941185435570347	27.45195271359973	26.8000245667271	21.806837284102826
28	20.830537351661324	28.295182874261577	29.355646593982517	21.518633180094582
29	22.727634610878965	26.495215174958314	29.032867096281095	21.744283117881622
30	23.96370493540998	26.217019737545467	27.638022924395898	22.181252402648656
31	25.307823365232167	26.60076111360064	24.69365518778761	23.397760333379587
32	25.65721681728952	28.504000054592726	25.655624529422067	20.183158598695687
33	25.72432037741772	27.85616636223639	24.221883039632043	22.197630220713847
34	23.21214506197412	28.047240906330256	26.651031916272956	22.089582115422672
35	24.46709537121917	26.873952217715797	26.0273100116237	22.631642399441336
36	24.282389978595102	28.239452798900867	25.14836710879193	22.3297901137121
37	24.042636919696374	30.70090237228145	25.356274410341683	19.90018629768049
38	23.246947925362644	29.356556472763916	24.4941642649658	22.90233133690764
39	25.126302548343	27.36778892632029	25.532108484847104	21.973800040489603
40	23.58883487747345	26.778642415364214	27.796341832359385	21.836180874802956
41	21.13307204647661	28.10820278468401	27.13122044315646	23.627504725682922
42	23.287210061439563	27.666911575705328	25.673139695964004	23.372738666891106
43	23.913206663042317	27.656447969719235	25.06602307907529	23.36432228816316
44	22.46786421878945	27.931686301092533	26.051649269026132	23.548800211091876
45	22.533602960745554	27.80316592321988	24.83127435347426	24.83195676256031
46	22.52655140018971	29.671602000823437	24.963661716167866	22.838184882818986
47	23.201681455988027	27.699212272445006	26.874862096497193	22.224244175069778
48	23.08635432044566	25.982043542249084	27.55181190985831	23.379790227446946
49	21.936722480147584	26.024352905584152	28.34704596480134	23.691878649466926
50	20.90901439655702	27.906664634604056	27.81658663524552	23.367734333593408
51	21.381696423493977	26.85006789970406	26.123074753365984	25.645160923435977
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	120.0
1	427.5
2	735.0
3	2425.5
4	4116.0
5	2982.0
6	1848.0
7	1769.0
8	1690.0
9	1592.5
10	1495.0
11	1519.5
12	1544.0
13	1529.0
14	1514.0
15	1483.5
16	1453.0
17	1381.0
18	1309.0
19	1293.0
20	1277.0
21	1292.0
22	1307.0
23	1595.5
24	1884.0
25	2171.0
26	2852.0
27	3246.0
28	4282.0
29	5318.0
30	6261.0
31	7204.0
32	7657.0
33	8110.0
34	9521.5
35	10933.0
36	12281.5
37	13630.0
38	13979.0
39	14328.0
40	17661.5
41	20995.0
42	22757.0
43	24519.0
44	26751.5
45	28984.0
46	32757.5
47	36531.0
48	34155.5
49	31780.0
50	31508.5
51	31237.0
52	29260.5
53	27284.0
54	25785.0
55	24286.0
56	24396.0
57	24506.0
58	24210.0
59	23914.0
60	23303.0
61	22692.0
62	21277.0
63	19862.0
64	16217.0
65	12572.0
66	10452.5
67	8333.0
68	6974.5
69	5616.0
70	4942.5
71	4269.0
72	3437.5
73	2606.0
74	2174.5
75	1445.0
76	1147.0
77	867.5
78	588.0
79	490.5
80	393.0
81	270.5
82	148.0
83	100.5
84	53.0
85	33.5
86	14.0
87	13.5
88	13.0
89	9.0
90	5.0
91	4.5
92	4.0
93	4.5
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	439619.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.70834339456999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.10296855663897	20.53270499324375
2	8.207107629810952	4.548107129659945
3	3.222180508627068	2.6784385203698697
4	1.987287000142521	2.202577224940553
5	1.4790762797389172	2.049137673288449
6	1.0776695499861382	1.7916262774132572
7	0.8225479074766199	1.5954007915193018
8	0.7402720369727058	1.6409369444670034
9	0.5535553085299615	1.380429051897178
>10	7.169061737705156	48.06376804313944
>50	0.5831648201276212	9.680304768987016
>100	0.05017355998277136	2.346063738480338
>500	0.0024675521303002307	0.49478379407818274
>1k	0.0024675521303002307	0.995721048515698
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1835	0.41740689096695094	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGC	1381	0.31413564927812493	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCG	1153	0.2622725587383621	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTC	981	0.22314777113819012	No Hit
GCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	647	0.14717289289134455	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	543	0.12351604457496151	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08097921154454198	0.0
2	0.0	0.0	0.0	0.37509752763188126	0.0
3	0.0	0.0	0.0	0.47108973906951246	0.0
4	0.0	0.0	0.0	0.6660312679843228	0.0
5	0.0	0.0	0.0	1.3259208541942	0.0
6	0.0	0.0	0.0	1.6157172460698923	0.0
7	0.0	0.0	0.0	1.8916379865292445	0.0
8	0.0	0.0	0.0	2.3731913315848496	0.0
9	0.0	0.0	0.0	2.5353772243692836	0.0
10	0.0	0.0	0.0	3.214601734683897	0.0
11	0.0	0.0	0.0	3.7691728519467995	0.0
12	0.0	0.0	0.0	4.556217997857235	0.0
13	0.0	0.0	0.0	4.765945056969785	0.0
14	0.0	0.0	0.0	4.862392207798116	0.0
15	0.0	0.0	0.0	5.056423857931527	0.0
16	0.0	0.0	0.0	5.377156128374797	0.0
17	0.0	0.0	0.0	5.734965959160091	0.0
18	0.0	0.0	0.0	6.152827789517741	0.0
19	0.0	0.0	0.0	6.410550954349107	0.0
20	0.0	0.0	0.0	6.668274119180472	0.0
21	0.0	0.0	0.0	6.977177965465551	0.0
22	0.0	0.0	0.0	7.336807553813643	0.0
23	0.0	0.0	0.0	7.657084884866213	0.0
24	0.0	0.0	0.0	7.928456231418569	0.0
25	0.0	0.0	0.0	8.167071941840549	0.0
26	0.0	0.0	0.0	8.381348394860094	0.0
27	0.0	0.0	0.0	8.595624847879641	0.0
28	0.0	0.0	0.0	8.831055982566722	0.0
29	0.0	0.0	0.0	9.065349768777054	0.0
30	0.0	0.0	0.0	9.346274842534104	0.0
31	0.0	0.0	0.0	9.628109795072552	0.0
32	0.0	0.0	0.0	9.902665717359804	0.0
33	0.0	0.0	0.0	10.151972503463226	0.0
34	0.0	0.0	0.0	10.414927471287637	0.0
35	0.0	0.0	0.0	10.70381398438193	0.0
36	0.0	0.0	0.0	10.990653270218075	0.0
37	0.0	0.0	0.0	11.280904601484467	0.0
38	0.0	0.0	0.0	11.562739554022915	0.0
39	0.0	0.0	0.0	11.839570173263667	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	20	7.028529E-4	45.0	1
CGGGTAT	40	6.7993824E-9	45.0	6
CGACGCA	25	3.8868624E-5	45.0	24
TCCGATG	25	3.8868624E-5	45.0	44
CGGCCTA	20	7.028529E-4	45.0	45
GCGCGAT	20	7.028529E-4	45.0	40
CGGTTGA	25	3.8868624E-5	45.0	30
ATTCGAA	20	7.028529E-4	45.0	11
TTATCGA	20	7.028529E-4	45.0	13
TTATACG	20	7.028529E-4	45.0	1
ATATCCG	20	7.028529E-4	45.0	40
TCATGCC	20	7.028529E-4	45.0	17
GATATAC	20	7.028529E-4	45.0	26
ATCCACG	20	7.028529E-4	45.0	43
GTCCGAT	25	3.8868624E-5	45.0	43
ACCGTTT	20	7.028529E-4	45.0	23
ATCGGAT	20	7.028529E-4	45.0	39
GCTAAGC	25	3.8868624E-5	45.0	38
CGTTTTA	45	3.8380676E-10	45.0	1
CGATACT	20	7.028529E-4	45.0	10
>>END_MODULE