SRR2934635.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934635.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1209874
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

N content graph

Sequence length distribution

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6824	0.5640256754009095	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	5458	0.4511213564387696	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG	5084	0.4202090465618734	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	4765	0.39384266460805006	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	1608	0.13290640182366098	RNA PCR Primer, Index 40 (96% over 27bp)
GAACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCT	1383	0.11430942395654424	RNA PCR Primer, Index 40 (96% over 25bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	25	3.8909275E-5	45.000004	2
TACGTAC	25	3.8909275E-5	45.000004	31
TAACGCG	40	6.8157533E-9	45.0	1
TAACGAA	20	7.033435E-4	45.0	1
ATCCGTT	20	7.033435E-4	45.0	12
AAACGAC	20	7.033435E-4	45.0	34
CGTAGTA	20	7.033435E-4	45.0	37
CGCTATC	20	7.033435E-4	45.0	30
CCGCGAT	20	7.033435E-4	45.0	44
CGTTTTT	3520	0.0	41.292614	1
TATAGCG	45	1.9284926E-8	40.0	1
CACGACC	805	0.0	39.130436	27
TCGAACG	35	6.2485615E-6	38.57143	1
TACGGCT	790	0.0	38.449368	7
AACACGT	775	0.0	37.741936	41
CGAACGC	30	1.1399575E-4	37.499996	21
CTATACG	30	1.1399575E-4	37.499996	1
AGACACG	835	0.0	37.45509	24
ACGACCA	820	0.0	37.317074	28
CTCACGA	55	2.750312E-9	36.81818	24