##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934635.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1209874
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17166911595753	31.0	31.0	33.0	28.0	34.0
2	31.22165200673789	31.0	31.0	34.0	28.0	34.0
3	30.990689939613546	31.0	31.0	34.0	28.0	34.0
4	34.96045290666631	35.0	35.0	37.0	32.0	37.0
5	35.19375075421077	35.0	35.0	37.0	33.0	37.0
6	35.16067210304544	36.0	35.0	37.0	32.0	37.0
7	31.012347566771417	35.0	35.0	37.0	0.0	37.0
8	33.05742085539486	36.0	35.0	37.0	17.0	37.0
9	36.432171449258355	39.0	35.0	39.0	32.0	39.0
10	36.183546385822	37.0	35.0	39.0	32.0	39.0
11	36.38339446917613	38.0	35.0	39.0	32.0	39.0
12	36.42034377133486	38.0	35.0	39.0	32.0	39.0
13	36.36796476327287	38.0	35.0	39.0	32.0	39.0
14	37.377372354476584	39.0	36.0	41.0	32.0	41.0
15	37.49255790272375	39.0	36.0	41.0	32.0	41.0
16	37.46711310433979	39.0	36.0	41.0	32.0	41.0
17	37.36655304601967	39.0	36.0	40.0	32.0	41.0
18	37.277530552768305	39.0	36.0	40.0	32.0	41.0
19	37.0699353817009	39.0	35.0	40.0	32.0	41.0
20	37.05983928904993	39.0	35.0	40.0	32.0	41.0
21	37.02567044171542	39.0	35.0	40.0	31.0	41.0
22	37.1521530341176	39.0	35.0	40.0	32.0	41.0
23	37.207652201799526	39.0	35.0	40.0	32.0	41.0
24	37.15220014646153	39.0	35.0	40.0	32.0	41.0
25	36.853203722040476	39.0	35.0	40.0	31.0	41.0
26	36.84293653719313	39.0	35.0	40.0	31.0	41.0
27	36.84543597101847	39.0	35.0	40.0	31.0	41.0
28	36.83223211673282	39.0	35.0	40.0	31.0	41.0
29	36.80777667757138	39.0	35.0	40.0	31.0	41.0
30	36.5608674952929	39.0	35.0	40.0	31.0	41.0
31	36.525709288735854	39.0	35.0	40.0	30.0	41.0
32	36.3559808707353	38.0	35.0	40.0	30.0	41.0
33	36.17459999966939	38.0	35.0	40.0	30.0	41.0
34	35.98589935811498	38.0	35.0	40.0	29.0	41.0
35	35.930393578174254	38.0	35.0	40.0	29.0	41.0
36	35.87388190836401	38.0	35.0	40.0	29.0	41.0
37	35.80591615325232	38.0	35.0	40.0	29.0	41.0
38	35.601949459199886	38.0	35.0	40.0	28.0	41.0
39	35.50106953286045	38.0	34.0	40.0	27.0	41.0
40	35.34435238710808	38.0	34.0	40.0	26.0	41.0
41	35.198967826401756	38.0	34.0	40.0	26.0	41.0
42	35.180636165418875	38.0	34.0	40.0	26.0	41.0
43	35.106739214166105	38.0	34.0	40.0	26.0	41.0
44	34.90114755751426	38.0	34.0	40.0	25.0	41.0
45	34.77621223367061	37.0	34.0	40.0	25.0	41.0
46	34.6175965431111	37.0	34.0	40.0	24.0	41.0
47	34.52316935482538	37.0	33.0	40.0	24.0	41.0
48	34.46930754772811	37.0	33.0	40.0	24.0	41.0
49	34.37098821860789	36.0	33.0	40.0	24.0	41.0
50	34.192643200862236	36.0	33.0	40.0	24.0	41.0
51	32.53732702744253	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	48.0
9	82.0
10	99.0
11	69.0
12	53.0
13	50.0
14	59.0
15	102.0
16	210.0
17	357.0
18	676.0
19	1222.0
20	2019.0
21	3208.0
22	4497.0
23	6575.0
24	9382.0
25	12804.0
26	16370.0
27	18869.0
28	20670.0
29	24098.0
30	29163.0
31	36998.0
32	47797.0
33	65492.0
34	97133.0
35	107013.0
36	127543.0
37	180491.0
38	225773.0
39	170942.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.40749532595956	20.244339493203427	23.842730730638067	12.505434450198946
2	33.42951414775423	24.606281315244395	24.15102729705738	17.813177239943993
3	32.681667677791246	24.529496459961948	26.908504521958488	15.880331340288329
4	27.829757478877966	25.124103832299895	27.722556233128408	19.32358245569373
5	26.314641028735224	29.114106096998533	25.064097583715327	19.50715529055092
6	25.83128491066012	35.00182663649273	24.78216739924984	14.384721053597316
7	70.84332748699451	16.820181275074926	9.135579407442428	3.200911830488133
8	83.04327558076295	5.28046722220661	8.406825834756347	3.269431362274088
9	77.40177902822938	5.940535956636807	10.070139535191268	6.58754547994254
10	42.58831911422181	26.857424822750136	16.396831405584383	14.157424657443668
11	32.35956802113278	24.612893574041593	24.124743568338523	18.902794836487104
12	27.50840170133419	23.04678007792547	28.071518191150485	21.37330002958986
13	25.941296366398486	24.313854169938356	30.47482630422672	19.270023159436438
14	22.365634768579206	25.748962288635013	30.930824201528424	20.954578741257354
15	20.65520872421426	24.170946726683937	34.32398745654506	20.849857092556746
16	25.43454938282829	25.236346925382314	29.057405977812568	20.271697713976828
17	25.70375096910918	24.063580174464448	28.404114808649496	21.828554047776876
18	27.299619629812693	22.705752830460032	29.577129519272255	20.41749802045502
19	26.516232268814772	24.548506704003888	26.946938276217193	21.98832275096415
20	28.371053514663508	25.109226250006202	27.421367844916084	19.09835239041421
21	26.07345888910746	26.415230015687584	28.71307260094853	18.798238494256427
22	27.068190571910794	21.395533749795433	28.589588667910874	22.9466870103829
23	24.264179575724416	25.61332833005751	28.87548620765468	21.247005886563393
24	23.86661751554294	23.479965682376843	29.69548895174208	22.957927850338134
25	24.488831068359186	26.480856684249765	27.135966224582063	21.894346022808985
26	23.93232683734009	25.098316022990826	27.027029260898246	23.942327878770847
27	23.70271615060742	25.26618474320466	27.784628812587098	23.246470293600822
28	21.140052600518732	26.281827694454137	28.88309030527146	23.695029399755676
29	23.87273385493035	25.232875489513784	27.833394221216423	23.060996434339444
30	24.25409588105869	24.417749286289318	28.5590896242088	22.76906520844319
31	25.202624405516605	25.580267036071525	26.555905821597953	22.661202736813916
32	26.36621664735336	24.977311687002118	26.90957901401303	21.74689265163149
33	25.143362036046728	25.375948239238134	25.886497271616715	23.594192453098422
34	22.902632836146573	26.359769695026092	29.65631131836869	21.08128615045864
35	21.745735506341983	28.01862012077291	27.715613361391352	22.52003101149376
36	24.456348347017954	28.781344173029588	24.991776003120986	21.770531476831472
37	23.457484002466373	27.60618047829774	24.99896683456294	23.93736868467295
38	22.828906150557827	29.383886255924168	25.392396232996163	22.394811360521842
39	24.273519391275457	25.843600242669893	25.997417912939696	23.885462453114954
40	25.065585341944697	25.051286332295756	27.463107728573387	22.420020597186152
41	21.712095639711244	25.969646425991467	26.22248267174929	26.095775262548
42	23.794709201123425	24.890856403228767	27.05694973195556	24.257484663692253
43	23.347141933788144	24.161441604662965	26.89056876997109	25.600847691577798
44	23.451367663078965	25.027234240920954	27.27589815137775	24.24549994462233
45	23.582455693733397	24.605124169954887	26.51061184883715	25.301808287474564
46	23.547328068873288	26.360926840315603	26.416552467447023	23.675192623364087
47	21.167576127762064	26.82965333580191	28.721172617975093	23.281597918460932
48	23.099512841833118	26.716914323309698	26.798410412985152	23.38516242187203
49	24.190039623960843	25.168405966241114	28.165164306365785	22.47639010343226
50	22.146438389452126	26.987438361350023	27.295321661594514	23.570801587603338
51	22.88494504386407	24.53792708992837	26.417709612736534	26.159418253471024
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	413.0
1	963.5
2	1514.0
3	6784.0
4	12054.0
5	8146.5
6	4239.0
7	3945.0
8	3651.0
9	3701.5
10	3752.0
11	3654.5
12	3557.0
13	3646.5
14	3736.0
15	3736.5
16	3737.0
17	3580.5
18	3424.0
19	3530.0
20	3636.0
21	3689.5
22	3743.0
23	4379.0
24	5015.0
25	5883.0
26	7735.0
27	8719.0
28	10754.5
29	12790.0
30	15075.5
31	17361.0
32	19563.0
33	21765.0
34	24236.5
35	26708.0
36	29777.5
37	32847.0
38	37610.5
39	42374.0
40	44231.0
41	46088.0
42	51006.0
43	55924.0
44	62919.0
45	69914.0
46	85056.5
47	100199.0
48	95968.5
49	91738.0
50	93274.5
51	94811.0
52	91728.0
53	88645.0
54	83517.5
55	78390.0
56	75610.5
57	72831.0
58	68515.5
59	64200.0
60	59760.5
61	55321.0
62	52005.0
63	48689.0
64	44701.5
65	40714.0
66	35033.5
67	29353.0
68	24008.0
69	18663.0
70	15647.0
71	12631.0
72	10392.0
73	8153.0
74	6874.0
75	4316.5
76	3038.0
77	2352.5
78	1667.0
79	1274.5
80	882.0
81	580.0
82	278.0
83	214.0
84	150.0
85	124.5
86	99.0
87	69.5
88	40.0
89	36.0
90	32.0
91	25.5
92	19.0
93	17.0
94	15.0
95	11.0
96	7.0
97	4.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1209874.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.885197537898005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.51700501504351	21.2355321225417
2	8.409131826054693	4.857988678356296
3	3.6378253616921596	3.1523791254255986
4	2.3279423443362104	2.6897229789195527
5	1.62263265396623	2.343503237062912
6	1.2120374960491767	2.1005965498031838
7	0.9523375990503266	1.9255921769935334
8	0.7309327511404181	1.6890509522888175
9	0.543272746873406	1.4123286549355312
>10	6.560585731385221	46.33081743698201
>50	0.4503697422313304	7.782918467057982
>100	0.03055186673543078	1.7027283659133734
>500	0.0036775395128335994	0.724055861731129
>1k	8.486629645000614E-4	0.6337633747417488
>5k	8.486629645000614E-4	1.419022017246675
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6824	0.5640256754009095	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	5458	0.4511213564387696	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG	5084	0.4202090465618734	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	4765	0.39384266460805006	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	1608	0.13290640182366098	RNA PCR Primer, Index 40 (96% over 27bp)
GAACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCT	1383	0.11430942395654424	RNA PCR Primer, Index 40 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.26532349649633E-5	0.0	0.0	0.05744399830064949	0.0
2	8.26532349649633E-5	0.0	0.0	0.3078833002444883	0.0
3	8.26532349649633E-5	0.0	0.0	0.40739779514230406	0.0
4	8.26532349649633E-5	0.0	0.0	0.6747810102539603	0.0
5	8.26532349649633E-5	0.0	0.0	1.5458634535497084	0.0
6	8.26532349649633E-5	0.0	0.0	1.9986378746877773	0.0
7	8.26532349649633E-5	0.0	0.0	2.405539750420292	0.0
8	1.653064699299266E-4	0.0	0.0	3.0843707691875353	0.0
9	1.653064699299266E-4	0.0	0.0	3.4101898214194204	0.0
10	1.653064699299266E-4	0.0	0.0	4.384836768126267	0.0
11	1.653064699299266E-4	0.0	0.0	4.991428859534134	0.0
12	1.653064699299266E-4	0.0	0.0	5.970787040633984	0.0
13	1.653064699299266E-4	0.0	0.0	6.205439574699514	0.0
14	1.653064699299266E-4	0.0	0.0	6.330163306261643	0.0
15	1.653064699299266E-4	0.0	0.0	6.535473941914613	0.0
16	1.653064699299266E-4	0.0	0.0	6.834595999252815	0.0
17	1.653064699299266E-4	0.0	0.0	7.185541634914049	0.0
18	1.653064699299266E-4	0.0	0.0	7.578227154232589	0.0
19	1.653064699299266E-4	0.0	0.0	7.840485868776418	0.0
20	1.653064699299266E-4	0.0	0.0	8.091669049834941	0.0
21	1.653064699299266E-4	0.0	0.0	8.387484977774545	0.0
22	1.653064699299266E-4	0.0	0.0	8.733388766102916	0.0
23	1.653064699299266E-4	0.0	0.0	9.035155726959998	0.0
24	1.653064699299266E-4	0.0	0.0	9.291876674761173	0.0
25	1.653064699299266E-4	0.0	0.0	9.519586337089647	0.0
26	1.653064699299266E-4	0.0	0.0	9.744733749134207	0.0
27	1.653064699299266E-4	0.0	0.0	9.995421010782941	0.0
28	1.653064699299266E-4	0.0	0.0	10.230569464258261	0.0
29	1.653064699299266E-4	0.0	0.0	10.478281209448257	0.0
30	1.653064699299266E-4	0.0	0.0	10.869478970537427	0.0
31	1.653064699299266E-4	0.0	0.0	11.14595404149523	0.0
32	1.653064699299266E-4	0.0	0.0	11.421685233338348	0.0
33	1.653064699299266E-4	0.0	0.0	11.694606215192657	0.0
34	1.653064699299266E-4	0.0	0.0	11.961080244719698	0.0
35	1.653064699299266E-4	0.0	0.0	12.292271757224306	0.0
36	2.4795970489488987E-4	0.0	0.0	12.56709376348281	0.0
37	3.306129398598532E-4	0.0	0.0	12.863157651127308	0.0
38	3.306129398598532E-4	0.0	0.0	13.210383891215118	0.0
39	3.306129398598532E-4	0.0	0.0	13.778459575129311	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	25	3.8909275E-5	45.000004	2
TACGTAC	25	3.8909275E-5	45.000004	31
TAACGCG	40	6.8157533E-9	45.0	1
TAACGAA	20	7.033435E-4	45.0	1
ATCCGTT	20	7.033435E-4	45.0	12
AAACGAC	20	7.033435E-4	45.0	34
CGTAGTA	20	7.033435E-4	45.0	37
CGCTATC	20	7.033435E-4	45.0	30
CCGCGAT	20	7.033435E-4	45.0	44
CGTTTTT	3520	0.0	41.292614	1
TATAGCG	45	1.9284926E-8	40.0	1
CACGACC	805	0.0	39.130436	27
TCGAACG	35	6.2485615E-6	38.57143	1
TACGGCT	790	0.0	38.449368	7
AACACGT	775	0.0	37.741936	41
CGAACGC	30	1.1399575E-4	37.499996	21
CTATACG	30	1.1399575E-4	37.499996	1
AGACACG	835	0.0	37.45509	24
ACGACCA	820	0.0	37.317074	28
CTCACGA	55	2.750312E-9	36.81818	24
>>END_MODULE