##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934631.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	703256
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.126857076228287	31.0	31.0	33.0	28.0	34.0
2	31.177009510050393	31.0	31.0	34.0	28.0	34.0
3	30.951976236249674	31.0	31.0	34.0	28.0	34.0
4	34.93015203567406	35.0	35.0	37.0	32.0	37.0
5	35.14022347480861	35.0	35.0	37.0	33.0	37.0
6	35.09120149703664	36.0	35.0	37.0	32.0	37.0
7	30.976086944156894	35.0	35.0	37.0	0.0	37.0
8	32.936370254928505	35.0	35.0	37.0	17.0	37.0
9	36.16527978431752	39.0	35.0	39.0	32.0	39.0
10	35.962633806181536	37.0	35.0	39.0	32.0	39.0
11	36.24573555006996	37.0	35.0	39.0	32.0	39.0
12	36.33360113529071	38.0	35.0	39.0	32.0	39.0
13	36.209851035753694	38.0	35.0	39.0	32.0	39.0
14	37.33830354806784	39.0	36.0	40.0	32.0	41.0
15	37.426861626491636	39.0	36.0	41.0	32.0	41.0
16	37.47364259956545	39.0	36.0	40.0	32.0	41.0
17	37.25806391982436	39.0	36.0	40.0	32.0	41.0
18	37.208579805931265	39.0	36.0	40.0	32.0	41.0
19	36.99260866597654	39.0	35.0	40.0	31.0	41.0
20	36.93474922361132	39.0	35.0	40.0	31.0	41.0
21	37.07594389525294	39.0	35.0	40.0	32.0	41.0
22	37.12876249900463	39.0	35.0	40.0	32.0	41.0
23	37.1762004163491	39.0	35.0	40.0	32.0	41.0
24	37.06814588144289	39.0	35.0	40.0	32.0	41.0
25	36.83446284141195	39.0	35.0	40.0	31.0	41.0
26	36.7411070790722	39.0	35.0	40.0	31.0	41.0
27	36.768457005699204	39.0	35.0	40.0	31.0	41.0
28	36.75630495864948	39.0	35.0	40.0	31.0	41.0
29	36.733282048073534	39.0	35.0	40.0	31.0	41.0
30	36.53379565904877	39.0	35.0	40.0	30.0	41.0
31	36.52846616310419	39.0	35.0	40.0	30.0	41.0
32	36.363797820423855	38.0	35.0	40.0	30.0	41.0
33	36.17219618460418	38.0	35.0	40.0	30.0	41.0
34	36.112018951846835	38.0	35.0	40.0	30.0	41.0
35	35.979256486969184	38.0	35.0	40.0	29.0	41.0
36	35.93283669104849	38.0	35.0	40.0	29.0	41.0
37	35.81342924909279	38.0	35.0	40.0	29.0	41.0
38	35.731786148998374	38.0	35.0	40.0	28.0	41.0
39	35.660311465526064	38.0	35.0	40.0	28.0	41.0
40	35.508671095589655	38.0	35.0	40.0	27.0	41.0
41	35.28914790631008	38.0	34.0	40.0	26.0	41.0
42	35.29832948456892	38.0	34.0	40.0	26.0	41.0
43	35.22229174013446	38.0	34.0	40.0	26.0	41.0
44	35.06997736243985	38.0	34.0	40.0	26.0	41.0
45	35.00842509697749	38.0	34.0	40.0	26.0	41.0
46	34.87003452512314	38.0	34.0	40.0	25.0	41.0
47	34.746240344909964	37.0	34.0	40.0	24.0	41.0
48	34.70353043557396	37.0	34.0	40.0	24.0	41.0
49	34.60079828682585	37.0	34.0	40.0	24.0	41.0
50	34.44274488948548	37.0	33.0	40.0	24.0	41.0
51	32.75812364203078	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	26.0
9	43.0
10	45.0
11	42.0
12	28.0
13	32.0
14	39.0
15	51.0
16	116.0
17	232.0
18	445.0
19	834.0
20	1412.0
21	2158.0
22	3025.0
23	4178.0
24	5629.0
25	7313.0
26	9445.0
27	10603.0
28	11906.0
29	13832.0
30	16683.0
31	21144.0
32	27914.0
33	37627.0
34	56572.0
35	60449.0
36	70577.0
37	102467.0
38	133305.0
39	105077.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.52914443673428	19.232256816863273	22.259888291034844	11.978710455367604
2	33.18108910553198	27.798696349551232	22.939583878416965	16.08063066649982
3	31.682203920051872	27.590663997178837	25.384355057048925	15.342777025720364
4	27.67043580147201	24.257311704414892	29.928646182897833	18.14360631121526
5	28.921047243109193	28.541100253677183	23.846934828853218	18.690917674360406
6	25.28638219936979	37.998822619359096	23.939788640267558	12.775006541003561
7	67.27578577360165	17.25587837146075	12.203521903830184	3.2648139511074206
8	77.4291296483784	9.469809002696032	9.328182056036493	3.772879292889076
9	72.27353908107432	7.3610463330565254	10.101158042021682	10.264256543847475
10	41.309565791120164	25.070813473329768	18.674422969729374	14.945197765820698
11	33.41912475684531	25.255810117510553	23.631508298542776	17.693556827101368
12	30.049085965850274	22.264438554381336	27.225647559352495	20.460827920415895
13	25.21372018155551	24.101465184797572	31.608830923589704	19.07598371005722
14	21.52743808797934	28.435875413789574	27.8958160328529	22.140870465378185
15	20.04405223702322	25.630353669218607	34.75704437644328	19.56854971731489
16	22.9701559602762	24.50530105679866	29.761566200643863	22.762976782281275
17	22.897636138191498	24.63626332373986	30.32551446414961	22.140586073919028
18	23.093724049279352	24.91738428111527	29.845461681094793	22.143429988510587
19	24.235271366330327	27.074351303081666	26.589890452409932	22.100486878178074
20	26.32398443809936	25.350939060598133	29.773652837657977	18.551423663644535
21	25.109206320315785	28.295101641507504	27.097529207002857	19.498162831173854
22	23.385225294913944	24.710062907390764	28.479813894229128	23.424897903466164
23	22.780751248478506	28.796057196810267	28.058345751760385	20.364845802950846
24	23.70758301386693	24.25361461544587	28.427343670014903	23.6114587006723
25	21.73774557202498	29.446744855358503	27.362866438395123	21.452643134221393
26	22.39895571456198	26.132020203169258	28.666090299976112	22.80293378229265
27	23.50580727359596	27.937621577348786	26.80688113574573	21.749690013309518
28	20.52382062861888	26.66482760189746	30.678586460691413	22.132765308792244
29	24.18848897129921	25.32335308906003	28.39776695826271	22.090390981378047
30	22.765536305413676	28.31387147781178	28.65741636047186	20.263175856302684
31	24.423822903750555	26.01797922804782	26.813137747847154	22.745060120354466
32	25.243154697578124	27.90704949548955	27.28636513588224	19.563430671050085
33	23.23023194967409	27.477476196434868	25.827436950413507	23.464854903477537
34	24.557913476742467	25.62679877597916	27.50321362348846	22.312074123789916
35	22.415592614922588	26.711467801198996	26.413425552003822	24.459514031874594
36	25.166795590794816	28.140392687726802	26.18747653770462	20.50533518377376
37	22.04076467175538	29.403232962107683	28.379281513417588	20.176720852719352
38	22.43877051884378	28.543517581080003	26.891914182033283	22.125797718042932
39	24.095777355614455	26.057651836600044	27.254939879645534	22.591630928139967
40	23.165675088445745	27.07804839205069	27.217542402766558	22.538734116737004
41	21.951607949310066	27.011216399149102	25.91019486502781	25.12698078651302
42	21.836002821163277	26.161739110651027	28.28187743865676	23.720380629528933
43	25.23391197515556	25.669457494852516	25.785062622999305	23.311567906992618
44	22.720033671948762	26.220750338425837	27.931222769517788	23.127993220107616
45	21.922031237557874	25.631206843596072	26.680469132151025	25.76629278669503
46	23.642172978261115	28.335343032978034	25.572906594469153	22.449577394291694
47	21.17052680673894	26.386266167654455	30.995682937650017	21.44752408795659
48	23.374702810925182	25.91246999670106	27.397704392141698	23.315122800232064
49	21.72196184604184	24.692999419841424	30.218014492588757	23.36702424152798
50	21.735470440351733	25.408101743888427	28.592859499243517	24.263568316516317
51	21.739309725050337	25.104656056969297	26.48423902533359	26.671795192646776
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	249.0
1	595.0
2	941.0
3	3459.5
4	5978.0
5	4323.5
6	2669.0
7	2507.0
8	2345.0
9	2268.5
10	2192.0
11	2246.5
12	2301.0
13	2308.0
14	2315.0
15	2321.0
16	2327.0
17	2282.0
18	2237.0
19	2300.0
20	2363.0
21	2401.5
22	2440.0
23	2742.0
24	3044.0
25	3820.0
26	5221.5
27	5847.0
28	7217.5
29	8588.0
30	9946.5
31	11305.0
32	12466.5
33	13628.0
34	16302.5
35	18977.0
36	19831.5
37	20686.0
38	23234.0
39	25782.0
40	28515.0
41	31248.0
42	33744.0
43	36240.0
44	39877.0
45	43514.0
46	63578.0
47	83642.0
48	69227.0
49	54812.0
50	53717.5
51	52623.0
52	48193.5
53	43764.0
54	41616.0
55	39468.0
56	37903.0
57	36338.0
58	34600.5
59	32863.0
60	32228.0
61	31593.0
62	28864.5
63	26136.0
64	21910.5
65	17685.0
66	14871.5
67	12058.0
68	9979.0
69	7900.0
70	6325.5
71	4751.0
72	3942.0
73	3133.0
74	2530.0
75	1659.5
76	1392.0
77	1047.5
78	703.0
79	491.0
80	279.0
81	253.0
82	227.0
83	147.0
84	67.0
85	45.0
86	23.0
87	27.5
88	32.0
89	22.0
90	12.0
91	12.0
92	12.0
93	7.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	703256.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.985519696313396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.52682126451221	22.04739947234345
2	8.33522223860491	4.998719412176738
3	3.560102154464162	3.2025453982071865
4	2.19184895918025	2.628949205473736
5	1.5696727397029522	2.353372642656454
6	1.143174220830066	2.0567203869010595
7	0.926752747343623	1.9452413931359331
8	0.7171773623492105	1.7203948739577872
9	0.5943321943369456	1.6039223747499276
>10	7.2261116786630675	47.265035114353246
>50	0.17022515154412465	3.1329340161940027
>100	0.03244623054052139	1.6947087599914616
>500	0.0018809409008997907	0.3588513016206232
>1k	0.0028214113513496857	1.4459803921884051
>5k	0.0014107056756748428	3.545225256050031
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGC	9376	1.3332271605219153	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCG	9253	1.3157370857838397	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTC	6514	0.9262629824701104	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2796	0.3975792598996667	No Hit
GCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	2154	0.30628960151068746	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCT	1661	0.23618710682880772	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTC	1281	0.182152729589225	No Hit
GAACTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCT	1230	0.17490074738075467	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATTACCGTCGTA	1133	0.1611077616117033	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGC	716	0.10181214237774011	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8439145915569865E-4	0.0	0.0	0.0819047402368412	0.0
2	2.8439145915569865E-4	0.0	0.0	0.589827886288919	0.0
3	2.8439145915569865E-4	0.0	0.0	0.7023047083849978	0.0
4	2.8439145915569865E-4	0.0	0.0	1.030634647980252	0.0
5	2.8439145915569865E-4	0.0	0.0	2.454440488243257	0.0
6	2.8439145915569865E-4	0.0	0.0	2.7745230755229957	0.0
7	2.8439145915569865E-4	0.0	0.0	3.2551446414961265	0.0
8	2.8439145915569865E-4	0.0	0.0	3.848954008213225	0.0
9	2.8439145915569865E-4	0.0	0.0	3.994562435300943	0.0
10	2.8439145915569865E-4	0.0	0.0	5.872399240106021	0.0
11	2.8439145915569865E-4	0.0	0.0	6.407766161966624	0.0
12	2.8439145915569865E-4	0.0	0.0	8.375186276405747	0.0
13	2.8439145915569865E-4	0.0	0.0	8.613932906366957	0.0
14	2.8439145915569865E-4	0.0	0.0	8.739918322772931	0.0
15	2.8439145915569865E-4	0.0	0.0	9.067679479449874	0.0
16	2.8439145915569865E-4	0.0	0.0	9.419756105884629	0.0
17	2.8439145915569865E-4	0.0	0.0	9.794868440510994	0.0
18	2.8439145915569865E-4	0.0	0.0	10.168985405030316	0.0
19	2.8439145915569865E-4	0.0	0.0	10.461766412231109	0.0
20	2.8439145915569865E-4	0.0	0.0	10.710608938992344	0.0
21	2.8439145915569865E-4	0.0	0.0	11.001257010249468	0.0
22	2.8439145915569865E-4	0.0	0.0	11.3496365477152	0.0
23	2.8439145915569865E-4	0.0	0.0	11.661187391220267	0.0
24	2.8439145915569865E-4	0.0	0.0	11.920268010511109	0.0
25	2.8439145915569865E-4	0.0	0.0	12.158588053283584	0.0
26	2.8439145915569865E-4	0.0	0.0	12.37771167256305	0.0
27	2.8439145915569865E-4	0.0	0.0	12.6309622669412	0.0
28	2.8439145915569865E-4	0.0	0.0	12.86828693960663	0.0
29	2.8439145915569865E-4	0.0	0.0	13.118978010852379	0.0
30	2.8439145915569865E-4	0.0	0.0	13.41175901805317	0.0
31	2.8439145915569865E-4	0.0	0.0	13.674110139124302	0.0
32	2.8439145915569865E-4	0.0	0.0	13.942860068026437	0.0
33	2.8439145915569865E-4	0.0	0.0	14.18587256987498	0.0
34	2.8439145915569865E-4	0.0	0.0	14.45234736710387	0.0
35	2.8439145915569865E-4	0.0	0.0	14.725789755082076	0.0
36	2.8439145915569865E-4	0.0	0.0	15.000369708896903	0.0
37	2.8439145915569865E-4	0.0	0.0	15.269830616446926	0.0
38	2.8439145915569865E-4	0.0	0.0	15.5505249866336	0.0
39	2.8439145915569865E-4	0.0	0.0	15.8323569226569	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	20	7.031416E-4	45.0	1
CGTACTC	20	7.031416E-4	45.0	31
TACGGCT	1020	0.0	41.911766	7
ACGGCTG	1120	0.0	41.38393	8
CGGCTGT	1115	0.0	40.358746	9
CGTTTTT	2060	0.0	40.194176	1
CGTTTTA	140	0.0	40.178574	1
TGATACC	1030	0.0	39.975727	4
GCTACGA	110	0.0	38.863632	10
GTAAGCG	35	6.2448307E-6	38.57143	1
GATACCT	1070	0.0	38.481308	5
ATGATAC	3275	0.0	38.40458	3
TGATACG	2210	0.0	38.076923	4
GAATGAT	3410	0.0	38.071846	1
ATACGGC	2200	0.0	38.045456	6
AATGATA	3330	0.0	37.972973	2
TGAAACG	125	0.0	37.8	4
CGACCAC	785	0.0	37.54777	12
CTAGACG	30	1.1394713E-4	37.500004	44
ACCTGCG	30	1.1394713E-4	37.500004	14
>>END_MODULE