FastQCFastQC Report
Sat 14 Jan 2017
SRR2934629.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934629.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936786
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47140.5032099113351395No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC42710.4559205624336828No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG40050.4275256034996253No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC37710.4025465794749281No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC17940.19150585085601193No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTT12850.13717113620399962No Hit
GAACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT9690.10343877897406664No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG453.8380676E-1045.01
CGGTTTA207.032618E-445.01
CGCATAG351.2116107E-745.02
CCGTAGT253.8902508E-545.041
TCTCGAC207.032618E-445.012
TATAGCG207.032618E-445.01
ACCGTTA207.032618E-445.010
TCTTCGA302.1650703E-644.99999621
ATCCGTC302.1650703E-644.99999626
TCCGTCA302.1650703E-644.99999627
CGTTTTT28700.042.334491
CGAGGAT650.041.5384645
CGCGCAA850.039.70588318
CGACCGG403.4573168E-739.3752
TCGCGCG403.4573168E-739.3751
TACGGCT5150.039.320397
CGCGGGT750.039.04
TAGCACG356.2470535E-638.5714261
CGCTCCG356.2470535E-638.57142618
GACCGAT4150.038.4939779
Produced by FastQC (version 0.11.5)