##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934629.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	936786
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18244828594791	31.0	31.0	33.0	28.0	34.0
2	31.217346330965665	31.0	31.0	34.0	28.0	34.0
3	30.978959975917657	31.0	31.0	34.0	28.0	34.0
4	34.94041115046553	35.0	35.0	37.0	32.0	37.0
5	35.19227443621062	35.0	35.0	37.0	33.0	37.0
6	35.17543494458713	36.0	35.0	37.0	32.0	37.0
7	31.111722421129265	35.0	35.0	37.0	0.0	37.0
8	33.15935443100132	37.0	35.0	37.0	17.0	37.0
9	36.5689282290726	39.0	35.0	39.0	32.0	39.0
10	36.156687866812696	37.0	35.0	39.0	32.0	39.0
11	36.36256519632018	38.0	35.0	39.0	32.0	39.0
12	36.586337754834084	38.0	35.0	39.0	32.0	39.0
13	36.623533016078376	39.0	35.0	39.0	32.0	39.0
14	37.72309470893032	39.0	37.0	41.0	33.0	41.0
15	37.87524792215084	40.0	37.0	41.0	33.0	41.0
16	37.813115268588554	39.0	37.0	41.0	33.0	41.0
17	37.681991404653786	39.0	36.0	41.0	33.0	41.0
18	37.59288247262448	39.0	36.0	40.0	33.0	41.0
19	37.40610235421964	39.0	36.0	40.0	32.0	41.0
20	37.383903047227435	39.0	36.0	40.0	32.0	41.0
21	37.42729823033222	39.0	36.0	40.0	32.0	41.0
22	37.53070711987583	39.0	36.0	41.0	33.0	41.0
23	37.58910039219203	39.0	36.0	41.0	33.0	41.0
24	37.52969621663859	39.0	36.0	41.0	33.0	41.0
25	37.220682204900584	39.0	35.0	40.0	32.0	41.0
26	37.23101434052174	39.0	35.0	40.0	32.0	41.0
27	37.27404764802207	39.0	36.0	40.0	32.0	41.0
28	37.27267059926173	39.0	36.0	41.0	32.0	41.0
29	37.26424818475084	39.0	36.0	41.0	32.0	41.0
30	37.0350901913564	39.0	35.0	41.0	31.0	41.0
31	37.03465572713512	39.0	35.0	40.0	31.0	41.0
32	36.87903960990023	39.0	35.0	40.0	31.0	41.0
33	36.7379027867624	39.0	35.0	40.0	31.0	41.0
34	36.687197502951584	39.0	35.0	41.0	30.0	41.0
35	36.62634475750065	39.0	35.0	41.0	30.0	41.0
36	36.58187141994009	39.0	35.0	41.0	30.0	41.0
37	36.492677089538056	39.0	35.0	40.0	30.0	41.0
38	36.36556481416247	39.0	35.0	40.0	30.0	41.0
39	36.320814999370185	39.0	35.0	40.0	30.0	41.0
40	36.26237689290831	39.0	35.0	40.0	30.0	41.0
41	36.149315852286435	39.0	35.0	40.0	29.0	41.0
42	36.17019468694024	39.0	35.0	40.0	29.0	41.0
43	36.12907643794848	39.0	35.0	40.0	29.0	41.0
44	35.970071072795704	39.0	35.0	40.0	29.0	41.0
45	35.89894383562521	39.0	35.0	40.0	28.0	41.0
46	35.81206166616495	38.0	35.0	40.0	28.0	41.0
47	35.752476019069455	38.0	35.0	40.0	28.0	41.0
48	35.69506162560072	38.0	35.0	40.0	28.0	41.0
49	35.6040130830307	38.0	35.0	40.0	28.0	41.0
50	35.46439635092754	38.0	34.0	40.0	27.0	41.0
51	33.938101124483076	37.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	34.0
9	57.0
10	88.0
11	44.0
12	31.0
13	22.0
14	26.0
15	36.0
16	91.0
17	195.0
18	358.0
19	750.0
20	1310.0
21	1949.0
22	2835.0
23	4097.0
24	5863.0
25	8256.0
26	10530.0
27	11986.0
28	13283.0
29	15060.0
30	18735.0
31	23776.0
32	31177.0
33	42061.0
34	60744.0
35	72311.0
36	91421.0
37	143601.0
38	201168.0
39	174880.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.62806233227226	21.326642370829624	24.735638662405286	13.30965663449283
2	31.721545795944863	24.978703780799457	26.27419709517435	17.025553328081333
3	30.936521254587497	24.568578095744385	28.42687657586685	16.068024073801272
4	27.667898538193352	25.44198995288145	28.50715104623681	18.382960462688384
5	25.880617344836494	29.510688673827318	25.88467376754136	18.724020213794827
6	25.842935312867617	35.25885314255337	24.967495244378117	13.9307163002009
7	72.490835687126	16.13292683707912	8.517633696489915	2.8586037793049854
8	85.53031321988159	4.4846955441264065	7.261850625436332	2.723140610555666
9	79.28139404303651	7.01419534450771	8.786852066533871	4.917558545921907
10	34.96529623628022	39.703411451494794	15.07847042974596	10.25282188247903
11	23.525436972798484	25.003149064994567	33.76352763598089	17.707886326226056
12	22.771049097659443	23.452207868179073	34.01801478672824	19.758728247433247
13	21.768472201762197	25.182058655872314	35.445555334943094	17.6039138074224
14	19.110661346348046	29.206563718928336	31.866082541797162	19.816692392926452
15	18.09292623929051	28.28885145593551	35.42687444090753	18.191347863866454
16	23.094922426253166	27.89484471373398	30.821660443260253	18.1885724167526
17	23.077095515944944	26.35276359808964	30.369476059633683	20.200664826331735
18	23.125452344505575	25.460564098951092	32.10925440815725	19.304729148386077
19	22.47172780122675	27.11771952185451	30.234333134782116	20.176219542136625
20	23.326565512294163	27.63939683129338	31.284519623478573	17.749518032933885
21	22.926901127899008	27.270689357014298	31.425533686455605	18.376875828631086
22	22.351849835501383	24.080739891501366	31.791892705484496	21.77551756751275
23	19.830996620359397	27.42643463928795	31.97912863770381	20.763440102648843
24	20.18326490788718	26.735988795733494	32.264679446533144	20.816066849846177
25	19.99389401634952	29.447493878004156	30.060867690166166	20.497744415480163
26	20.47468685484198	28.48110454255294	29.37981566761245	21.664392934992623
27	21.2076183888316	27.684764716808317	30.68150036401056	20.426116530349514
28	19.31903337581902	27.36163862397602	31.977420670249128	21.341907329955827
29	20.670782868232447	26.631162293202504	30.553189308977718	22.144865529587335
30	21.75363423449966	26.313266850700163	31.3468604355744	20.58623847922578
31	22.7067868221771	27.02388805981302	29.086152013373383	21.18317310463649
32	21.893687565783434	29.232716970578128	28.982392990501566	19.891202473136875
33	21.319383509147233	28.576216980185443	28.664390800033306	21.440008710634018
34	20.732056200669096	27.60587796999528	29.932129643269644	21.729936186065974
35	20.773154167547336	29.22310965364555	28.76750933510962	21.23622684369749
36	21.877888866827643	29.639746964621587	28.180075278665566	20.302288889885205
37	21.109623756119326	29.544421031057254	28.756621042586033	20.589334170237386
38	21.148693511645135	30.081897039451917	27.81563772302319	20.953771725879765
39	21.06030619586544	28.81287722062456	28.3747835684991	21.7520330150109
40	21.76996667328504	28.03532503688142	29.651916232736188	20.542792057097355
41	19.810180767005484	28.650086572600358	28.70143234420668	22.838300316187475
42	21.546116188756024	28.135561376877966	29.16343754069766	21.154884893668353
43	22.05295553093236	27.540227970956018	28.958908437999714	21.447908060111914
44	21.521670904560914	27.597871872551465	29.19215274352947	21.688304479358145
45	21.057210504853828	26.955569361625813	28.53170307839784	23.455517055122517
46	21.86390488329245	27.67238195276189	28.37318234901034	22.09053081493532
47	20.089540193811604	27.822256096910074	30.418900367853492	21.66930334142483
48	20.982913920575243	27.75681959380264	29.186495101335847	22.07377138428627
49	21.464560742795047	26.63692668336205	30.247783378487725	21.650729195355183
50	20.212620598514494	27.047159116383035	29.924123545825836	22.816096739276635
51	20.306131816658233	26.946602532488743	28.57493600459443	24.172329646258593
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	187.0
1	484.0
2	781.0
3	4819.5
4	8858.0
5	6138.5
6	3419.0
7	3208.0
8	2997.0
9	2948.5
10	2900.0
11	2908.5
12	2917.0
13	2885.0
14	2853.0
15	2783.0
16	2713.0
17	2727.0
18	2741.0
19	2851.0
20	2961.0
21	3536.5
22	4112.0
23	4952.5
24	5793.0
25	7011.5
26	9752.0
27	11274.0
28	13764.0
29	16254.0
30	19129.0
31	22004.0
32	25553.0
33	29102.0
34	32838.5
35	36575.0
36	39801.5
37	43028.0
38	46474.5
39	49921.0
40	52327.5
41	54734.0
42	58974.0
43	63214.0
44	66476.0
45	69738.0
46	81286.0
47	92834.0
48	86170.5
49	79507.0
50	77317.5
51	75128.0
52	67393.5
53	59659.0
54	54254.5
55	48850.0
56	43921.0
57	38992.0
58	34600.5
59	30209.0
60	25940.0
61	21671.0
62	19267.0
63	16863.0
64	13829.0
65	10795.0
66	8415.5
67	6036.0
68	4850.0
69	3664.0
70	3009.5
71	2355.0
72	1784.5
73	1214.0
74	1012.5
75	619.5
76	428.0
77	322.5
78	217.0
79	173.5
80	130.0
81	86.5
82	43.0
83	32.0
84	21.0
85	21.0
86	21.0
87	16.5
88	12.0
89	10.5
90	9.0
91	7.0
92	5.0
93	4.5
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	936786.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.541919119031103
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.28220123440266	22.79919345081059
2	9.145073109849639	5.769063127370071
3	4.10720945962609	3.8864780574133673
4	2.4085537716169005	3.0388163303271027
5	1.6704809278301445	2.634508715775123
6	1.1982034583345529	2.267618194255909
7	0.8719937712984925	1.925304990461717
8	0.6733488977693295	1.6990973177863147
9	0.5355625786921299	1.5203404386258201
>10	6.938435497870302	48.052824498934335
>50	0.14376236755015034	2.7245897858639214
>100	0.021199936976558562	1.1702017205233748
>500	0.001987494091543342	0.4390346097880361
>1k	0.001987494091543342	2.072928762064407
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4714	0.5032099113351395	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC	4271	0.4559205624336828	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG	4005	0.4275256034996253	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC	3771	0.4025465794749281	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	1794	0.19150585085601193	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTT	1285	0.13717113620399962	No Hit
GAACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	969	0.10343877897406664	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07781926715386438	0.0
2	0.0	0.0	0.0	0.4261378799426977	0.0
3	0.0	0.0	0.0	0.5590390975099969	0.0
4	0.0	0.0	0.0	0.8407469795663044	0.0
5	0.0	0.0	0.0	1.775432169140017	0.0
6	0.0	0.0	0.0	2.2593206986440872	0.0
7	0.0	0.0	0.0	2.6485237823793266	0.0
8	0.0	0.0	0.0	3.269049708257809	0.0
9	0.0	0.0	0.0	3.477101493831035	0.0
10	0.0	0.0	0.0	4.393212537335101	0.0
11	0.0	0.0	0.0	5.038183747408693	0.0
12	0.0	0.0	0.0	5.9870664164494345	0.0
13	0.0	0.0	0.0	6.243368282617375	0.0
14	0.0	0.0	0.0	6.369757874263706	0.0
15	0.0	0.0	0.0	6.577382667973262	0.0
16	0.0	0.0	0.0	6.902323476226161	0.0
17	0.0	0.0	0.0	7.362407209330626	0.0
18	0.0	0.0	0.0	7.847576714425706	0.0
19	0.0	0.0	0.0	8.176787441315305	0.0
20	0.0	0.0	0.0	8.486356542476083	0.0
21	0.0	0.0	0.0	8.837450602378771	0.0
22	0.0	0.0	0.0	9.24127815744471	0.0
23	0.0	0.0	0.0	9.646173192169822	0.0
24	0.0	0.0	0.0	9.949550911307385	0.0
25	0.0	0.0	0.0	10.234567980307135	0.0
26	1.0674796591750944E-4	0.0	0.0	10.511578951863072	0.0
27	1.0674796591750944E-4	0.0	0.0	10.803641386613378	0.0
28	1.0674796591750944E-4	0.0	0.0	11.092287886454324	0.0
29	1.0674796591750944E-4	0.0	0.0	11.414026255729697	0.0
30	1.0674796591750944E-4	0.0	0.0	11.802909095567184	0.0
31	1.0674796591750944E-4	0.0	0.0	12.138204456514082	0.0
32	1.0674796591750944E-4	0.0	0.0	12.441048435822056	0.0
33	1.0674796591750944E-4	0.0	0.0	12.757556154767471	0.0
34	1.0674796591750944E-4	0.0	0.0	13.073316637951464	0.0
35	1.0674796591750944E-4	0.0	0.0	13.415764112614834	0.0
36	1.0674796591750944E-4	0.0	0.0	13.733232563253507	0.0
37	1.0674796591750944E-4	0.0	0.0	14.059561095063334	0.0
38	1.0674796591750944E-4	0.0	0.0	14.409801171238682	0.0
39	1.0674796591750944E-4	0.0	0.0	14.850136530648408	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	45	3.8380676E-10	45.0	1
CGGTTTA	20	7.032618E-4	45.0	1
CGCATAG	35	1.2116107E-7	45.0	2
CCGTAGT	25	3.8902508E-5	45.0	41
TCTCGAC	20	7.032618E-4	45.0	12
TATAGCG	20	7.032618E-4	45.0	1
ACCGTTA	20	7.032618E-4	45.0	10
TCTTCGA	30	2.1650703E-6	44.999996	21
ATCCGTC	30	2.1650703E-6	44.999996	26
TCCGTCA	30	2.1650703E-6	44.999996	27
CGTTTTT	2870	0.0	42.33449	1
CGAGGAT	65	0.0	41.538464	5
CGCGCAA	85	0.0	39.705883	18
CGACCGG	40	3.4573168E-7	39.375	2
TCGCGCG	40	3.4573168E-7	39.375	1
TACGGCT	515	0.0	39.32039	7
CGCGGGT	75	0.0	39.0	4
TAGCACG	35	6.2470535E-6	38.571426	1
CGCTCCG	35	6.2470535E-6	38.571426	18
GACCGAT	415	0.0	38.493977	9
>>END_MODULE