FastQCFastQC Report
Sat 14 Jan 2017
SRR2934627.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934627.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898962
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC55510.6174899495195569No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCG52440.5833394515007309No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGC48940.5444056589711245No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36850.4099172156331413No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT18350.2041243122623648No Hit
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC18340.20401307285513737TruSeq Adapter, Index 20 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCT10070.11201808307803889No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCCG207.032465E-445.01
GTAATCG207.032465E-445.01
TATAGCG750.045.01
CTCACGA501.0804797E-940.524
TACGGCT6000.040.1257
TCGAATG451.927583E-840.0000041
CGTTTTT20800.039.699521
GCTATCG301.1397239E-437.50000420
ACGATAC301.1397239E-437.50000445
ATACGCG301.1397239E-437.5000041
CGGGATA1100.036.818186
ATAGCGG2150.036.6279072
CGAGATA2350.036.3829819
GCTACGA1300.036.34615710
TACGAAT1250.036.00000412
TCACGAC504.8768925E-836.025
TATCGAC250.002106865236.015
CTATGCG458.697116E-735.0000041
ACTACGG458.697116E-735.0000042
GTACGAG458.697116E-735.0000041
Produced by FastQC (version 0.11.5)